Purpose and Application
- General buffer for electrophoresis of DNA
Features
- Available for electrophoresis of DNA
- Sterilized, DNase free
- Stable for 1 year at room temperature
琼脂糖凝胶电泳所用缓冲液的类型可由DNA片段的大小和迁移后实验的含量来决定。
如果你想分离大于或等于15,000 bp的大DNA,使用TAE缓冲液。如果你不想提取DNA,或者不关心迁移后1000bp或更小的DNA的恢复率,可以使用TBE缓冲液。
上图显示了EzDNA Ladder应用于使用EzRunTAE或EzRunTBE缓冲液的1.5%琼脂糖凝胶,并使用dive – mini在150V下电泳20分钟的结果。可以看到,TAE缓冲液适用于聚合物分离,TBE缓冲液适用于低分子分离。
Specifications
WSE-7050 EzRun TAE | |
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Major components | Tris, Acetic acid, EDTA, 50x concentrated solution |
Package | 200 mL/bottle |
Preparation | 50x dilute with distilled water |
Applicable amount | For 25L, 220 mL per 1 run (About 110 runs with ATTO Submerge Mini chamber) |
Storage | For 1 year at room temperature (unopened) |
Ordering Information
Code No. | Description | Unit |
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2332391 | WSE-7050 EzRun TAE | 1 pk |