Reaction of NO release and peroxynitrite production
1. M. Feelisch, et al., On the Mechanism of NO Release from Sydnonimines. J Cardiovasc Pharmacol. 1989;14:S13-S22.2. M. Feelisch, The Biochemical Pathways of Nitric Oxide Formation from Nitrovasodilators: Appropriate Choice of Exogenous NO Donors and Aspects of Preparation and Handling of Aqueous NO Solutions. J Cardiovasc Pharmacol. 1991;17:S25-S33.3. N. Hogg, et al., Production of Hydroxyl Radicals from the Simultaneous Generation of Superoxide and Nitric Oxide. Biochem J. 1992;281:419-424.4. M. E. Murphy, et al., Nitric Oxide Hyperpolarizes Rabbit Mesenteric Arteries via ATP-sensitive Potassium Channels. J Physiol. 1995;486:47-58.5. H. Kankaanranta, et al., 3-Morpholino-sydnonimine-induced Suppression of Human Neutrophil Degranulation in Not Mediated by Cyclic GMP, Nitric Oxide or Peroxynitrite: Inhibition of the Increase in Intracellular Free Calcium Concentration by N-Morpholinoiminoacetoni. Mol Pharmacol. 1997;51:882-888.6. S. Yamamoto, et al., Subarachnoid Hemorrhage Impairs Cerebral Blood Flow Response to Nitric Oxide but Not to Cyclic GMP in Large Cerebral Arteries. Brain Res. 1997;757:1-9.7. S. Pfeiffer, et al., Interference of carboxy-PTIO with Nitric-oxide and Peroxynitrite-mediated Reactions. Free Radic Biol Med. 1997;22:787-794.8. M. B. Herrero, et al., Tyrosine Nitration in Human Spermatozoa: A Physiological Function of Peroxynitrite, the Reaction Product of Nitric Oxide and Superoxide. Mol Hum Reprod. 2001;7:913-921.9. P. D. Lu, et al., Cytoprotection by Pre-emptive Conditional Phosphorylation of Translation Initiation Factor 2. EMBO J. 2004;23:169-179.
细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐，称为WST。WST-8是高度稳定的WST，用于Cell Counting Kit-8（CCK-8）。由于WST-8甲maz是水溶性的，因此不会形成晶体。因此，不需要诸如MTT测定的增溶过程。此外，CCK-8的检测灵敏度高于其他四唑盐，例如MTT，XTT，MTS或WST-1。