CST Phospho-NF-kappaB p65 (Ser536) (93H1) Rabbit mAb 3033S
货号： 3033S
品牌： CST
规格：100ul
使用与人 NF-κB p65 的 Ser536 周围残基相对应的合成磷酸肽，对动物进行免疫接种来产生单克隆抗体。
Flow cytometric analysis of HeLa cells, untreated (blue) or TNF-α-treated (green), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb compared to a nonspecific negative control antibody (red).流式细胞仪研究未经处理的HeLa细胞(蓝色)和经TNF-α处理的HeLa细胞(绿色)。所用抗体为Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb 并与非特异性的阴性对照抗体比对(红色)。
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or TNF-α treated (#2169, 20 ng/ml for 5 minutes), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (upper) or NF-κB p65 Antibody #3034 (lower).Western免疫印迹。用Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (上图) 或NF-κB p65 Antibody #3034 (下图)研究未经处理的和经TNF-α (#2169, 20 ng/ml, 5 min) 处理的HeLa和 NIH/3T3 细胞的细胞提取液。
Confocal immunofluorescent analysis of HeLa cells, untreated (left) and TNF-α treated (#8902 at 20 ng/ml for 20 min, right), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® phalloidin 555 (red).共聚焦免疫荧光分析未经处理(左图)和经TNF-α(#8902 20 ng/ml,20 min, 右图)处理的HeLa细胞。所用的抗体为Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (绿色), 肌动蛋白用Alexa Fluor® phalloidin 555 (红色)标记。
Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24h) and treated with 1 μg/ml LPS for the indicated times, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (upper) and NF-κB p65 (C22B4) Rabbit mAb #4764 (lower).Western免疫印迹。用Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (上图) 和 NF-κB p65 (C22B4) Rabbit mAb #4764 (下图) 研究经 TPA 分化24小时(#9905, 80 nM) 并用1 μg/ml LPS 处理一定时间的THP-1细胞的细胞提取液。