Tissue-Tek® O.C.T. Compound 冰冻切片OCT包埋剂
(美国樱花Sakura)
产品关联词:
Sakura;Tissue-Tek® O.C.T. Compound; OCT冰冻切片包埋剂;恒冷切片机;多聚甲醛;切片石蜡;包埋盒;
基本介绍(美国Sakura OCT Compound 冰冻切片包埋剂):
OCT包埋剂(optimal cutting temperature compound)是一种聚乙二醇和聚乙烯醇的水溶性混合物,广泛用于免疫组化实验,用途是在冰冻切片时支撑组织,以增加组织的连续性,减少皱折及碎裂。又因OCT混合物为水溶性,故在漂片时可溶于水,因此在以后的染色中不会增加背景染色。
O.C.T. Compound is a water-soluble blend of glycols and resins that provides a convenient specimen matrix for cryostat sectioning at temperatures of -10oC and below. O.C.T. leaves no residue on slides during staining procedure, eliminating undesirable background staining. Dissected tissue, mounted in O.C.T. Compound can be frozen between -20 and -80℃. Developed and optimized for chromogenic IHC experiments. O.C.T. Compound will not dull microtome knives.
利用OCT混合物预先浸润组织,然后再进行恒冷箱切片,能使切片质量得到改善。
包埋流程(樱花Sakura,产地:USA)美国Sakura OCT Compound 冰冻切片包埋剂
1. 将放在蔗糖溶液中的组织块取出,放入20g/L 蔗糖与OCT包埋剂1:1体积比例混合液中,室温浸泡2h;
2. 移入OCT包埋剂中室温浸泡4h,更换新的OCT包埋剂,室温浸泡6h.
3. 将标本置于托物台,用恒冷箱切片机切片(一般-22℃),片厚10μm,贴于预处理的载玻片上,-20℃冰箱保存。
适用性:
与众多注明品牌Leica,Sakura,Thermo,Microm,Anglia,Bio-med,Leitz,Yamato等恒冷切片机兼容。
运输与保存:室温运输与保存。
订购信息
品名 | 产地 | 货号 | 规格 | ||
Tissue-Tek® O.C.T. Compound 冰冻切片包埋剂 | USA | 4583 | 118ML | ||
Tissue-Tek® O.C.T. Compound 冰冻切片包埋剂 | USA | 4583 | 118ML*12 |
相关产品(石蜡切片)
品名 | 产地 | 货号 | 规格 | ||
Paraffin (50-52℃) 切片石蜡 | China | MM1506-500G | 500g | ||
Paraffin (52-54℃) 切片石蜡 | China | MM1507-500G | 500g | ||
Paraffin (54-56℃) 切片石蜡 | China | MM1508-500G | 500g | ||
Paraffin (56-58℃) 切片石蜡 | China | MM1509-500G | 500g | ||
Paraffin (58-60℃) 切片石蜡 | China | MM1560-500G | 500g | ||
Paraffin (60-62℃) 切片石蜡 | China | MM1561-500G | 500g | ||
Paraffin (62-64℃) 切片石蜡 | China | MM1562-500G | 500g | ||
Slide Box,50-place 50片切片盒 | China | MM1563-1EA | 1 EA | ||
Slide Box,100-place 100片切片盒 | China | MM1564-1EA | 1 EA |
注1:切片石蜡熔点的选择应根据所在地区和季节的不同而考虑,一般情况,夏秋季节选用60-62℃的石蜡为好,其它季节可选用58-60℃的石蜡,这两种熔点适合切3-5μm厚切片。当然,也需要把切片的厚度作为考虑因素之一。总的来说,石蜡熔点越高,硬度越硬,则切片越难切,柔软度不适合,但可用来切较薄的切片;反而言之,熔点越低,硬度降低,切片支撑力降低,很难切出薄切片。