PT-5006 | BETHYL抗体金畔生物官网

Catalog #: PT-2501

Cryopreserved ampule of Human Mesenchymal Stem Cells (hMSCs) containing ≥750,000 cells

含有≥750,000 个细胞的人间充质干细胞 (hMSCs) 冻存安瓿

Product Overview

Poietics^TM Normal Human Bone Marrow Derived Mesenchymal Stem Cells (hMSC) are isolated from normal (non-diabetic) adult human bone marrow withdrawn from bilateral punctures of the posterior iliac crests of normal volunteers. Normal Human Bone Marrow Derived Mesenchymal Stem Cells (hMSC) have been reported to differentiate down many different lineages including chondrogenic, osteogenic, adipogenic and neural. Normal Human Bone Marrow Derived Mesenchymal Stem Cells (hMSC) have been cryopreserved after the second passage and are suggested to be used by no later than the fifth passage. Cryopreserved Poietics^TM Normal Human Bone Marrow Derived Mesenchymal Stem Cells (hMSC) are guaranteed to have at least 0.75 million viable cells per vial, differentiate down the adipogenic, chondrogenic, and osteogenic liniages when cultured in the recommended differentiation medium, to express CD29, CD44, CD105, and CD166 and to not express CD14, CD34, and CD45. All lots of this product manufactured after January 1, 2015 are also guaranteed to express CD90 and CD73 and to not express HLA-DR or CD19.

All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Lonza guarantees the performance of Clonetics^TM/Poietics^TM cells only if appropriate Clonetics^TM/Poietics^TM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.

PoieticsTM 正常人骨髓来源的间充质干细胞 (hMSC) 是从正常志愿者的髂后嵴双侧穿刺提取的正常（非糖尿病）成人骨髓中分离出来的。据报道，正常人骨髓来源的间充质干细胞 (hMSC) 可分化出许多不同的谱系，包括软骨形成、成骨、脂肪形成和神经。正常人骨髓来源的间充质干细胞（hMSC）在第二代后被冷冻保存，建议不迟于第五代使用。冷冻保存的 PoieticsTM 正常人骨髓来源的间充质干细胞 (hMSC) 保证每瓶至少有 75 万个活细胞，在推荐的分化培养基中培养时，可分化成脂肪、软骨和成骨细胞，以表达 CD29、CD44、 CD105 和 CD166 并且不表达 CD14、CD34 和 CD45。 2015 年 1 月 1 日之后生产的所有批次产品也保证表达 CD90 和 CD73，不表达 HLA-DR 或 CD19。

所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

Benefits

Ready to use
Each lot tested and verified to differentiate down each of the three lineages (adipogenic, chondrogenic, osteogenic)
Multiple donor availability

Applications

Regenerative medicine
Gene therapy
Cell differentiation and gene regulation
Cell-based screening assays

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Catalog #: PT-5006

Cryopreserved ampule of Human Adipose-Derived Stem Cells (hADSC) containing ≥1 million cells

Product Overview

PoieticsTM 正常人脂肪衍生干细胞 (hADSC) 是从在择期手术吸脂过程中收集的正常（非糖尿病）成人吸脂中分离出来的。

据报道，正常人 ADSC 可分化出许多不同的谱系，包括软骨形成、成骨、脂肪形成和神经。正常人 ADSC 在初次传代时已被冷冻保存。

相关的额外捐赠者信息，包括体重指数 (BMI) 可通过联系科学支持获得。冷冻保存的 PoieticsTM 正常人 ADSC 保证经过 5 次传代，表达 CD13、CD29、CD44、CD73、CD90、CD105 和 CD166，不表达 CD14、CD31 和 CD45。

所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

来自糖尿病捐赠者的人类 ADSC 作为单独的产品提供。这些细胞表现出与骨髓来源的间充质干细胞非常相似的表型和功能特征。

Recommended Media and Reagents

Growth Medium:

ADSC Human Adipose Derived Stem Cell Growth BulletKit^TM Medium

Differentiation Medium*:

Adipogenic: PGM^TM -2 Preadipocyte Growth Medium-2 BulletKit^TM Medium (provides both a plating medium and a differentiation medium appropriate to induce differentiation of human adipose derived mesenchymal stem cells into mature, functionally active adipocytes; Each bottle of the PGM-2^TM BulletKit^TM Medium contains enough differentiation medium to fully differentiate up to 20 million Adipose Derived Stem Cells (ADSC) [10 complete 96-well plates] into mature, functionally active adipocytes).

Chondrogenic: hMSC Chondrogenic Differentiation BulletKit^TM Medium, with the addition of TGF-β3 (provides a differentiation medium appropriate to induce chondrogenic differentiation of human adipose derived mesenchymal stem cells into mature, functionally active chondrocytes; Each bottle of the hMSC Chondrogenic Differentiation BulletKit^TM Medium, with the addition of TGF-β3 (sold separately), contains enough differentiation medium to fully differentiate approximately 12.5 million human adipose derived mesenchymal stem cells [approximately 25 micropellets] into mature, functionally active chondrocytes).

Osteogenic: hMSC Osteogenic Differentiation BulletKit^TM Medium (provides a differentiation medium appropriate to induce osteogenic differentiation of human adipose derived mesenchymal stem cells into mature, functionally active osteoblasts; Each bottle of the hMSC Osteogenic Differentiation BulletKit^TM Medium contains enough differentiation medium to fully differentiate approximately 200,000 human adipose derived mesenchymal stem cells [approximately 20 wells in a 24-well plate format] into mature, functionally active osteoblasts).

Lonza guarantees the performance of Clonetics^TM/Poietics^TM cells only if appropriate Clonetics^TM/Poietics^TM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

*Lonza does not routinely test differentiation of Lonza’s human adipose derived mesenchymal stem cells and cannot guarantee differentiation for all lots.

Benefits

All cells test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
Certificate of Analysis provided for every cell lot