lonza,CC-4461,R-CB-503,CC-3256,PNGM™ 原代神经元生长培养基 BulletKit™

Catalog #: CC-4461

含有 PNBMTM 基础培养基 (CC-3256) 和 PNGMTM SinglequotsTM 生长补充剂 (CC-4462) 的培养系统,是啮齿动物原代神经元生长所需的

Product Overview

CloneticsTM PNGMTM Primary Neuron Growth Medium Kit (PNGMTM) was specifically developed for culturing embryonic primary rat and mouse neurons. The media kit is recommended for use with all of our primary rat and mouse neurons.

CloneticsTM PNGMTM 原代神经元生长培养基试剂盒 (PNGMTM) 专为培养胚胎原代大鼠和小鼠神经元而开发。 建议将媒体套件与我们所有的主要大鼠和小鼠神经元一起使用。

Benefits

  • Serum-free medium
  • Allows for long-term maintenance and growth of neuronal cells
  • Promotes development of high quality neuronal networks without the need of an astrocyte feeder layer

Content & Storage

Content

1 x PNBMTM Basal Medium (CC-3256), 200 mL

1 x PNGMTM SinglequotsTM Growth Supplement Pack (CC-4462)

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ViaLightPlus Assay in PNGM™ Media of Rat Cortical Neurons

Comparison of survival of Clonetics™ Primary Rat Cortical Neurons cultured in PNGM™ Medium vs. Company A’s medium. Cryopreserved cells were thawed and plated in laminin-coated, 96 well plates at ~1000 cells per square mm (40% of the recommended seeding density) using either PNGM™ Medium or Company A’s medium. Cells were cultured without feeding for six days, at which time cell viability was assessed using the ViaLight™ Plus Cell Proliferation and Cytotoxicity BioAssay Kit. Results represent the average of 8 experiments.

PNGM™ Primary Neuron Growth Medium BulletKit™
在 PNGM™ 培养基与 A 公司培养基中培养的 Clonetics™ 原代大鼠皮质神经元的存活率比较。 使用 PNGM™ 培养基或 A 公司的培养基,将冷冻保存的细胞解冻并接种在层粘连蛋白涂层的 96 孔板中,每平方毫米约 1000 个细胞(推荐接种密度的 40%)。 细胞在不喂食的情况下培养六天,此时使用 ViaLight™ Plus 细胞增殖和细胞毒性生物测定试剂盒评估细胞活力。 结果代表 8 次实验的平均值。