Odyssey® EMSA Kit,Odyssey® EMSA 套件
10X Orange Loading Dye,10X 橙色负载染料
IRDye® 700 Dye-Labeled Oligonucleotides,IRDye® 700 染料标记寡核苷酸
Mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method.
A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey® Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol.
通过用 IRDye® 红外染料末端标记的寡核苷酸替换现有的 DNA 寡核苷酸，迁移率变化分析方案可以轻松转换为红外荧光分析。 结合和电泳条件与任何其他 EMSA 检测方法相同。
用 IRDye 700 红外染料末端标记的 DNA 寡核苷酸是蛋白质结合的良好底物。 使用 Odyssey® 红外成像系统，IRDye 红外染料标记的 DNA 检测在 9.1 fmol 至 0.18 fmol 的 50 倍稀释范围内是线性的。
Perform NIR Fluorescent EMSA and Save Time
Infrared fluorescent assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. If you are not satisfied that the electrophoresis has progressed far enough, you can place the gel back into the electrophoresis unit and run longer.
Other IRDye 700 oligonucleotides as well as IRDye 800 oligonucleotides are available through Integrated DNA Technologies, TriLink BioTechnologies, or Metabion International AG.
其他 IRDye 700 寡核苷酸和 IRDye 800 寡核苷酸可通过 Integrated DNA Technologies、TriLink BioTechnologies 或 Metabion International AG 获得。
Near-Infrared Fluorescence Detection for Gel Shift Assays Has Advantages over Use of Radioisotopes
| Infrared | Radioisotope |
|---|---|
| Easy access and disposal | Short half-life of the label |
| Dye is stable for a long time | Regulatory procedures Disposal limitations |
| Non-hazardous | Hazardous |
| Gel (glass plates) can be easily imaged on the Odyssey | Lengthy incubations with autoradiographic film |
| Detection of the probe is rapid | Time-consuming and inconvenient |
| Gel can be replaced back and run longer | Not possible |