标签：Near-Infrared Fluorescent Click Chemistry Reagents

Use Near-Infrared Click Chemistry Reagents for Biomolecule Labeling

Biomolecule labeling continues to be a cornerstone feature of many in vitro and in vivo biological experiments. Click Chemistry has become convenient and reliable method for labeling a wide variety of molecules for applications ranging from biomarker isolation to assay development.

生物分子标记仍然是许多体外和体内生物实验的基石特征。 Click Chemistry 已成为一种方便且可靠的方法，可用于标记从生物标志物分离到分析开发等各种应用的各种分子。

Click Chemistry reagents from LI-COR offer several benefits:

• Versatile alternatives to affinity-based detection
• Chemoselectivity and easy-to-perform, high yielding reactions
• Flexibility with a choice for copper-catalyzed and copper-free reactions

Click Chemistry Reactions

Click Chemistry utilizes pairs of reagents that exclusively react with each other and are effectively inert to naturally occurring functional groups such as amines.

Click Chemistry reactions can be categorized into two separate groups, copper-catalyzed or copper-free. Copper-catalyzed Click Chemistry is used for initiating reactions between azides and alkynes. Although they initiate and accelerate Click Reactions, copper catalysts are cytotoxic and inappropriate for use in living systems.

To address this limitation, copper-free methods have been developed to allow Click Chemistry with live cells and whole organisms.

Click Chemistry 使用成对的试剂，它们相互之间发生专门的反应，并且对天然存在的官能团（如胺）有效地呈惰性。

Click Chemistry 反应可分为两个独立的组，铜催化的或无铜的。 铜催化点击化学用于引发叠氮化物和炔烃之间的反应。 虽然它们会引发和加速点击反应，但铜催化剂具有细胞毒性，不适合在生命系统中使用。

为了解决这一限制，已开发出无铜方法以允许使用活细胞和整个生物体进行 Click Chemistry。