pFC334载体,哺乳系列质粒

    pFC334载体,哺乳系列质粒,pCDNA3.1(-),pCDNA3.1(+),pCDNA3.1-3×Myc,pCDNA4-Myc-HisB,pECMV-3×FLAG-MCS-IRES-Puro,pCAGGS-Myc,pEGFP-N1-Myc-6×His-FLAG,4xCSL-Luciferase,pHes1(2.5k)-Luc,pHes1(467)-luc,pMRX-IP-GFP-LC3-RFP-LC3ΔG,pFN10A (ACT),AAV pEF1a-DIO-FLPo-WPRE-hGHpA,pAct-dCas9-VPR,pFC332等不同型号的哺乳系列质粒由上海生物科技专业供应,本司为回馈新老客户,特在开学之际推出了质粒载体优惠活动专题,欢迎来电咨询!

    产品简介:   

    名称:pFC334载体,哺乳系列质粒

    系列属性:哺乳系列质粒

    供应商:上海

    产品规格:5μg

    复制子:pUC ori,F1 ori,SV40 ori

    终止子:cPPT/CTS , 3

    质粒分类:RNA文库质粒;RNA人源质粒;shRNA人源质粒

    质粒大小:7084bp

    原核抗性:Amp(100μg/ml)

    筛选标记:Puro

    克隆菌株:Stbl3

    培养条件:37℃,LB,有氧

    表达宿主:293T等哺乳细胞

    诱导方式:无需诱导

    5'测序引物:U6-F(ATGGACTATCATATGCTTACCGTA)

    3'测序引物:pLKO-R(CTGCACTGTACCCCCCAATC)

    pFC334载体,哺乳系列质粒 订购说明

    1、绝大部分产品备有现货,一般情况下都能订货当日发货。

    2、部分非常用产品,需提前1-2日预订,海外期货则需要提前3-6周预订。

    3、部分产品价格会因货期、批次等因素发生变化,若有变动以订货当日价格为准。

    4、每日订单截止时间为16点整,部分城市可到17点整,因超过截止时间造成当日不能发货的将于次日安排发货。

    5、请办理完货款后,将收据、底单等连同收货人的地址、姓名、电话等以传真、EMail、电话等形式通知我们。

    了解更多关于pFC334载体,哺乳系列质粒 及其他系列的质粒载体详情请来电咨询我司销售人员,我们将竭诚为您服务!

    生物,专业供应科研实验所需的培养基,抗体,动物血清血浆,标准品对照品,化学试剂,酶联免疫试剂盒,白介素试剂盒,金标检测试剂盒,微生物,蛋白质,ELISA种属涵盖广,凭借多年行业经验,完善的售后服务,高质量的产品,赢得客户一致好评,欢迎来电咨询与订购!

原创作者:上海生物科技有限公司

抗c-Myc磁珠


基本信息

产品名称 抗c-Myc磁珠
英文名称 Anti-c-Myc Magnetic Beads
规格或纯度 10mg/mL suspension in PBS with 0.05% Tween™-20 Detergent and 0.05% NaN3
运输条件 冰袋运输

一般描述

抗c-Myc磁珠是亲和颗粒,可使用手动或自动磁选机对细菌或哺乳动物细胞或体外系统中表达的重组c-Myc标记蛋白进行免疫沉淀。|抗c-Myc磁珠的特点:| •特异性-高度特异性的抗c-Myc单克隆抗体(克隆9E10)可实现高产量和高纯度的免疫沉淀。|•方便快捷-产品说明提供了易于遵循的优化方案,可在大约一小时内进行免疫沉淀。 •低的非特异性结合-稳定的预先封闭的珠子和特异性抗体可最大程度地减少c-Myc-tag IP或co-IP实验的脱靶结合。|•多功能-珠子与手动和自动工作流程兼容。磁珠表面涂有抗c-My

Anti-c-Myc Magnetic Beads are affinity particles for immunoprecipitation of recombinant c-Myc-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.|Features of Anti-c-Myc Magnetic Beads:|• Specific—highly specific anti-c-Myc monoclonal antibody (clone 9E10) enables high yield and high purity immunoprecipitation.|• Convenient and fast—product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately one hour.|• Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding for c-Myc-tag IP or co-IP experiments.|• Versatile—beads are compatible with manual and automated workflows.|The blocked magnetic bead surface is coated with anti-c-Myc antibody, a highly specific mouse IgG1 monoclonal antibody (clone 9E10) that recognizes the c-Myc-epitope tag (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc). Anti-c-Myc Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms.|Product Details:|Anti-c-Myc Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant c-Myc tagged proteins and the co-immunoprecipitation (Co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing c-Myc tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine (pH 2.0), 50 mM NaOH, or SDS-PAGE sample buffer.
•Do not centrifuge, dry or freeze Anti-c-Myc Magnetic Beads, as this can cause the beads to aggregate and lose binding activity. To ensure good dispersal of beads for optimal antibody binding, it is important to include 0.025% to 0.1% non-ionic (e.g.,sc-2
Anti-c-Myc Magnetic Beadsis for immunoprecipitation of recombinant c-Myc-tagged proteins

相关属性

储存温度 2-8°C储存
密度 2.0 g/mL
品牌 金畔生物