Lipophilic Peroxide Detection in Mitochondria (cell line used: HepG2 cell)
A: MitoPeDPP stained Mitochondria with t-BHP treatmentB: MitoRed stained MitochondriaC: Merged Image (A/B)
Selectivity of MitoPeDPP as a Peroxides Probe Even though MitoPeDPP reacts with various peroxides (H2O2, t-BHP, ONOO-) in homogeneous systems (data is not shown), the MitoPeDPP is specifically-oxidized by t-BHP in mitochondria (A) but not with ROS and RNS (B).
A: MitoPeDPP stained cells with t-BHP treatment (t-BHP) and without (control).B: MitoPeDPP stained cells with ROS or RNS exposure.*As ROS generators, PMA (O2–・), NOC7 (NO), and SIN-1 (ONOO–) were used in the experiments.*Abbreviationst-BHP: tert-butylhydroperoxidePMA: Phorbol Myristate AcetateNOC7: 1-Hydroxy-2-oxo-3-(N-methyl-3-aminopropyl)-3-methyl-1-triazineSIN-1: 3-(Morpholinyl)sydnonimine, hydrochloride