标签：lonza PT-3002

Product Overview

Stem cells possess the unique ability to differentiate into multiple lineages of cells including osteoblasts. Lonza’s hMSC Osteogenic Differentiation BulletKit^TM Medium is a serum containing medium designed to induce osteogenic differentiation of human bone marrow derived mesenchymal stem cells (hMSC) into mature, functionally active osteoblasts in as few as 14 days. Differentiation can then be validated by a variety of methods including the use of Lonza’s OsteoImage^TM Bone Mineralization Reagent which provides a quantitative value related to the amount of osteogenic differentiation.

干细胞具有分化成包括成骨细胞在内的多种细胞谱系的独特能力。 Lonza 的 hMSC 成骨分化 BulletKitTM 培养基是一种含有血清的培养基，旨在在短短 14 天内诱导人骨髓间充质干细胞 (hMSC) 成骨分化成成熟、功能活跃的成骨细胞。 然后可以通过多种方法验证分化，包括使用 Lonza 的 OsteoImageTM 骨矿化试剂，该试剂提供与成骨分化量相关的定量值。

Partial List of Cells Differentiated with hMSC Osteogenic Differentiation BulletKit^TM Medium*:

• Human bone marrow derived stem cells
• Human umbilical cord blood derived stem cells
• Human umbilical cord matrix derived stem cells
• C57BL/6 mice bone marrow derived stem cells
• Human adipose derived stem cells
• Human periodontal ligament derived stem cells
• Human hamstring tendon derived stem cells

The hMSC Osteogenic Differentiation BulletKit^TM Medium provides a differentiation medium guaranteed to induce osteogenic differentiation of bone marrow derived hMSCs into mature, functionally active osteoblasts; Each bottle of the hMSC Osteogenic Differentiation BulletKit^TM Medium contains enough differentiation medium to fully differentiate up to 750,000 bone marrow derived hMSCs [approximately 25 wells in a 6-well plate format] into mature, functionally active osteoblasts.

The hMSC Osteogenic Differentiation Medium is offered as a BulletKit^TM Medium (catalog no. PT-3002) which includes both the basal media and the necessary supplements for osteogenic differentiation of bone marrow derived hMSCs.

Lonza guarantees the performance of cells only if appropriate media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

*List of cells generated from customer data. Lonza only routinely uses this medium with Lonza’s human bone marrow derived mesenchymal stem cells and can therefore only guarantee the successful use of this media with these cells.

hMSC 成骨分化 BulletKitTM 培养基提供了一种分化培养基，可保证诱导骨髓来源的 hMSC 成骨分化为成熟的、功能活跃的成骨细胞；每瓶 hMSC 成骨分化 BulletKitTM 培养基都含有足够的分化培养基，可以将多达 750,000 个骨髓来源的 hMSCs [大约 25 个孔，以 6 孔板形式] 完全分化成成熟的、功能活跃的成骨细胞。

hMSC 成骨分化培养基以 BulletKitTM 培养基（目录号 PT-3002）的形式提供，其中包括基础培养基和骨髓衍生 hMSC 成骨分化所需的补充剂。

只有在专门使用适当的培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

*从客户数据生成的单元格列表。 Lonza 仅常规地将这种培养基与 Lonza 的人骨髓来源的间充质干细胞一起使用，因此只能保证这种培养基与这些细胞一起成功使用。