标签：lonza CC-5012

Product Overview

Trypsin is a clear, colorless-yellow, highly active bovine enzyme preparation, used to remove cells from the culture vessel surface into suspension.  It is generally used at a working concentration of 0.025%, prepared in a balanced salt solution, without calcium or magnesium at a pH of 7.6-7.8.  After treatment, trypsin activity can be halted by adding a serum media mixture or a trypsin inhibitor (from soybean, for example) in serum-free systems.

Trypsin/EDTA is also a component of Lonza’s ReagentPack^TM Subculture Reagents kit (CC-5034) which includes Trypsin Neutralizing reagent and HEPES buffer.

胰蛋白酶是一种透明、无色黄色、高活性的牛酶制剂，用于将细胞从培养容器表面去除成悬浮液。 它通常在 0.025% 的工作浓度下使用，在平衡盐溶液中制备，不含钙或镁，pH 为 7.6-7.8。 处理后，可以通过在无血清系统中添加血清培养基混合物或胰蛋白酶抑制剂（例如来自大豆）来停止胰蛋白酶活性。

胰蛋白酶/EDTA 也是 Lonza 的 ReagentPackTM Subculture Reagents 试剂盒 (CC-5034) 的一个组成部分，其中包括胰蛋白酶中和试剂和 HEPES 缓冲液。