标签：lonza CC-4540

Product Overview

InEpC’s are truly primary cells representing both villi (enterocytes, goblet, and enteroendocrine cells) and crypts structures.  In combination with human intestinal myofibroblasts (InMyoFib), InEpC are able to form very tight cell monolayer, representing a unique in vitro system to model human intestinal homeostasis.

Human intestinal epithelial cells (InEpC) are of pivotal importance in the digestion and absorption of nutrients and the protection of the organism from bacteria and toxins found in the intestine. The intestinal epithelium is a highly organized cellular system maintained in a dynamic steady state by cells in proliferation and differentiation, which die and exfoliate into the lumen. Intestinal cells are often difficult to isolate due to contamination issues and usually have to be cultured and used for assays immediately after isolation from tissue.

Cryopreserved InEpCs are sold at P0, express cytokeratins 8/18 at ≥ 90%, contain 0.8M viable cells / vial and have a seeding efficiency ≥ 50% coverage at certain cell density (see instructions).  Their life span is, post plating, that the cells can be used on days 5-7, and they maintain their properties for approximately 5 days after that (using Clonetics^TM reagents & protocol).  All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

InEpC 是真正的原代细胞，代表绒毛（肠细胞、杯状细胞和肠内分泌细胞）和隐窝结构。与人类肠道肌成纤维细胞 (InMyoFib) 结合，InEpC 能够形成非常紧密的细胞单层，代表了一种独特的体外系统来模拟人类肠道稳态。

人类肠道上皮细胞 (InEpC) 在营养物质的消化和吸收以及保护生物体免受肠道中发现的细菌和毒素的侵害方面至关重要。肠上皮是一种高度组织化的细胞系统，由增殖和分化中的细胞维持在动态稳定状态，这些细胞死亡并脱落进入管腔。由于污染问题，肠细胞通常难以分离，并且通常必须在从组织中分离后立即进行培养和用于检测。

冷冻保存的 InEpC 以 P0 销售，以 ≥ 90% 表达细胞角蛋白 8/18，包含 0.8M 活细胞/小瓶，并且在一定细胞密度下具有 ≥ 50% 的接种效率（见说明）。它们的寿命是，电镀后，细胞可以在第 5-7 天使用，并且在此之后它们保持其特性大约 5 天（使用 CloneticsTM 试剂和协议）。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。
Clonetics^TM Human Intestinal Myofibroblasts

H-InMyoFib share phenotypic characteristics of both fibroblasts and smooth muscle cells but do not express smooth muscle markers such as smoothelin, caldesmon, or (in the normal colon and small intestine) desmin. They play a fundamental role in growth and development of intestine, immunology, inflammation, fibrosis, water and electrolyte transport, contraction and wound healing. They are a useful tool in general gastrointestinal research.

Cryopreserved InMyoFib are guaranteed through 15 population doublings and stain ≥70% postitive for alpha smooth muscle actin and negative (≤10%) for desmin after thaw. They are cryopreserved at passage 2 with ≥500,000 cells per vial.  All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

H-InMyoFib 具有成纤维细胞和平滑肌细胞的表型特征，但不表达平滑肌标志物，如平滑素、caldesmon 或（在正常结肠和小肠中）结蛋白。 它们在肠道的生长和发育、免疫学、炎症、纤维化、水和电解质运输、收缩和伤口愈合中发挥着重要作用。 它们是一般胃肠道研究的有用工具。

冷冻保存的 InMyoFib 通过 15 次群体倍增和 α 平滑肌肌动蛋白染色≥70% 阳性和解冻后结蛋白阴性 (≤10%) 得到保证。 它们在第 2 代冷冻保存，每瓶≥500,000 个细胞。 所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

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