Culture system containing SABMTM Basal Medium (CC-3119) and SAGMTM SingleQuotsTM supplements (CC-4124) required for growth of Small Airway Epithelial cells
Product Overview
SAGMTM BulletKitTM is small airway epithelial cell growth medium that is serum-free and has been optimized to grow human small airway epithelial cells (SAEC) from normal and diseased tissue.
Published research shows SAGMTMTM BulletKitTM Small Airway Epithelial Cell Growth Medium is also suitable to support co-culture experiments with primary mammary epithelial cells (HMEC) as well as established breast cancer cell lines such as MCF-7 and MDA-MB-231. Refer to the relevant published data in the ‘Data’ tab.
SAGMTM BulletKitTM 是无血清的小气道上皮细胞生长培养基，经过优化，可从正常和患病组织中培养人小气道上皮细胞 (SAEC)。
已发表的研究表明 SAGMTMTM BulletKitTM 小气道上皮细胞生长培养基也适用于支持与原代乳腺上皮细胞 (HMEC) 以及已建立的乳腺癌细胞系（如 MCF-7 和 MDA-MB-231）的共培养实验。 请参阅“数据”选项卡中的相关已发布数据。
Content & Storage
Content
1 x SABMTM Basal Medium (CC-3119), 500 mL
1 x SAGMTM SingleQuotsTM Supplement Pack (CC-4124) containing:
1 x Orange Cap Vial with BPE, 2.0 mL
1 x Lilac Cap Vial with Insulin, 0.5 mL
1 x Natural Cap Vial with Hydrocortisone, 0.5 mL
1 x Red Cap Vial with GA-1000, 0.5 mL
1 x Amber Vial with Retinoic Acid, 0.5 mL
1 x Bottle with Fatty Acid Free BSA, 5 mL
1 x Natural Cap Vial with Transferrin, 0.5 mL
1 x Amber Vial with Triiodothyronine, 0.5 mL
1 x Amber Vial with Epinephrine, 0.5 mL
1 x Green Cap Vial with hEGF, 0.5 mL
SABM™ 小气道上皮细胞生长基础培养基 CC-3119
Co-culture Experiments with Lonza’s Small Airway Epithelial Cells and SAGM™ BulletKit™ Media
数据由 Furukawa 等人提供。 Lonza 的 SAEC 细胞与原代正常乳腺上皮细胞、乳腺癌细胞系 MCF-7 和 MDA-MB-231 在 Lonza 的 SAGM™ BulletKit™ 生长培养基中共培养。 乳腺癌细胞系在存在小气道上皮细胞的情况下表现出形态和增殖率的差异。 上图显示了 MCF-7 细胞系的荧光（红色）覆盖的相差成像。 MCF-7 细胞系在 SAGM™ 培养基中以紧密簇的形式存活。 当与 Lonza 的原代 SAEC 共培养时，MCF-7 细胞在保持聚集的同时表现出增强的生长。
