lonza 2W-501,原代细胞和干细胞,NK-人类自然杀伤细胞

Catalog #: 2W-501

Crypreserved ampule of Peripheral Blood Human Natural Killer Cells (CD56 negative immunomagnetic selection) containing ≥ 5 million cells

Product Overview

自然杀伤 (NK) 细胞是免疫系统的淋巴细胞,作为先天性和适应性免疫反应的一部分,对宿主防御和免疫调节至关重要。 它们对免疫治疗研究人员特别感兴趣,因为它们有望专门针对和破坏癌细胞和肿瘤,同时保持健康细胞完好无损。

Cells You Can Count On

自然杀伤细胞的传统特征是存在 CD56 细胞表面标志物且不存在 CD3。 此外,CD16 对 NK 细胞功能很重要,因为 CD16 诱导细胞因子表达和细胞毒效应活性。

使用 CD56 标记的阳性 (2W-502) 或阴性 (2W-501) 免疫磁性选择分离 Lonza NK 细胞。 基于 CD56+ 细胞的存在,纯度≥90%。 阳性和阴性选择的 NK 细胞都保证表达 CD56 和 CD16。 然而,NK 细胞的负免疫磁性分离导致 CD16+ 细胞的富集显着增加(通常 > 总细胞的 80%),而 CD16 的表达随阳性选择的 NK 细胞而变化。

Lonza NK 细胞保证表达 CD56 和 CD16,每瓶含有 ≥500 万个活细胞。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

Choices in Cell Type and Tissue Source

CD56和CD16的表达可以极大地影响细胞功能。 此外,选择技术也会影响目标细胞的功能。 这就是为什么 Lonza 提供阳性和阴性选择的 NK 细胞,并表征每批细胞中 CD56 和 CD16 的表达水平。

如果外周血衍生的自然杀伤细胞不是您想要的,我们还提供组织来源选择。 脐带血 MNC 或骨髓 MNC 可以去除谱系标记表达细胞,以分离 NK 细胞。 此外,脐带血 CD34+ 细胞或骨髓 CD34+ 细胞可分化为自然杀伤细胞。

Recommended Media and Reagents

  • LGM-3TM Lymphocyte Growth Medium is recommended for optimal performance of NK cells. Additional cytokines may be necessary depending on application.

Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate ofAnalysis is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule of Human Peripheral Blood NK containing ≥ 5 million cells

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