| 产品货号 | 产品名称 |
| 00193121 | Lonza LATITUDE (24X14,2%GEL,TBE ,EB,2X25W)5PK |
| 50001 | Lonza SeaKem LE 琼脂糖 |
| 50002 | Lonza SeaKem LE 琼脂糖 |
| 50004 | Lonza SeaKem LE 琼脂糖 |
| 50005 | Lonza SeaKem LE AGAROSE 1KG |
| 50010 | Lonza SeaKem ME 琼脂糖 |
| 50011 | Lonza SeaKem ME 琼脂糖 |
| 50014 | Lonza SeaKem ME 琼脂糖 |
| 50015 | Lonza SeaKem ME AGAROSE 1KG |
| 50025 | Lonza SeaKem HE AGAROSE 1KG |
| 50040 | Lonza SeaKem HGT 琼脂糖 |
| 50031 | Lonza SeaKem HEEO 琼脂糖 |
| 50045 | Lonza SeaKem HGT AGAROSE 1KG |
| 50070 | Lonza SeaKem GTG 琼脂糖 |
| 50071 | Lonza SeaKem GTG 琼脂糖 |
| 50074 | Lonza SeaKem GTG AGAROSE 500G |
| 50075 | Lonza SeaKem GTG AGAROSE 1KG |
| 50080 | Lonza NuSieve GTG 琼脂糖 |
| 50041 | Lonza SeaKem HGT 琼脂糖 |
| 50081 | Lonza NuSieve GTG 琼脂糖 |
| 50115 | Lonza SeaPlaque GTG AGAROSE 1 KG |
| 50111 | Lonza SeaPlaque GTG琼脂糖 |
| 50155 | Lonza SeaKem GOLD AGAROSE 1KG |
| 50180 | Lonza MetaPhor 琼脂糖 |
| 50184 | Lonza MetaPhor 琼脂糖 500G |
| 50202 | Lonza IsoGel AGAROSE 25G |
| 50302 | Lonza SeaPrep 琼脂糖 |
| 50320 | Lonza DNA分子量Marker，20bp-1000bp |
| 50321 | Lonza DNA LADDER – 100bp W/TYII LOADING BUFFER |
| 50322 | Lonza DNA LADDER-100bp EXT.RA NGE W/TYII L.B. |
| 50323 | Lonza DNA LADDER – 500bp W/TYII LOADING BUFFER |
| 50326 | Lonza SimplyLoad 20bp Extnd Range DNA Ladder |
| 50327 | Lonza SimplyLoad 100 bp DNA Ladder |
| 50328 | Lonza SimplyLoad 100bp Extended Rng DNA Ladder |
| 50329 | Lonza SimplyLoad 500 bp DNA Ladder |
| 50330 | Lonza 20 bp DNA Ladder |
| 50331 | Lonza SimplyLoad 20 bp DNA Ladder |
| 50334 | Lonza DNA QuantLadder |
| 50401 | Lonza LAMBDA DNA LADDERS (48.5K B-1MB) 5 PLUGS |
| 50461 | Lonza DNA MARKER 50-1000 bp |
| 50512 | Lonza SYBR GREEN I-2x500uL |
| 50530 | Lonza SYBR GREEN II Filter |
| 50536 | Lonza GelStar FILTER |
| 50543 | Lonza SYPRO RED 1x500uL |
| 50552 | Lonza ProSieve COLOR PROTEIN M ARKERS 100uL |
| 50564 | Lonza SYPRO RUBY Protein Gel Stain – 200mL |
| 50565 | Lonza SYPRO RUBY Protein Blot Stain – 200mL |
| 50571 | Lonza FORMALDEHYDE SAMPLE BUFFE R- 5 X 1ML |
| 50575 | Lonza RNA标准MARKER |
| 50611 | Lonza LONG RANGER 50%胶溶液 |
| 50615 | Lonza LONG RANGER 50%胶溶液 |
| 50617 | Lonza ProSieve 50 GEL SOLUTION 125ML |
| 50618 | Lonza ProSieve 50 GEL SOLUTION 250ML |
| 50620 | Lonza MDE 胶溶液 |
| 50631 | Lonza DNA MARKER 50-2500 bp |
| 50880 | Lonza AccuGENE Tris-甘氨酸-SDS缓冲液，10X |
| 50882 | Lonza AccuGENE Tris-甘氨酸-SDS缓冲液，10X |
| 51200 | Lonza AccuGENE 分子生物学级水 |
| 51201 | Lonza AccuGENE 0.5 M EDTA溶液 |
| 51202 | Lonza 5M氯化钠 |
| 51203 | Lonza AccuGENE 醋酸钠溶液，3M，pH5.2 |
| 51205 | Lonza AccuGENE SSC 缓冲液 (20X) |
| 51206 | Lonza 10% SDS- 100 mL |
| 51213 | Lonza 10% SDS- 500 mL |
| 51214 | Lonza AccuGENE SSPE 缓冲液(20X) |
| 51237 | Lonza AccuGENE Tris-HCl缓冲液，1M |
| 51238 | Lonza ACCUGENE 1M TRIS SOL(PH8. 0)-1L |
| 51244 | Lonza AccuGENE 分子生物学级水 |
| 53734 | Lonza GelBond 胶片，85mmx100mm |
| 53740 | Lonza GelBond 胶片，102mmx16.5m |
| 53745 | Lonza GelBond膜片 |
| 53746 | Lonza GelBond Film – 100mm x 1 50mm x 100shts |
| 53748 | Lonza GelBond Film – 110mm x 2 05mm x 100shts |
| 53749 | Lonza GelBond Film – 160mm x 1 80mm x 100shts |
| 53750 | Lonza GelBond 胶片，127mmx16.5m |
| 54801 | Lonza Reliant GELS (1%SKG,TAE, 8W) 20PK |
| 54803 | Lonza Reliant GELS (1%SKG,TAE, EB,8W) 20PK |
| 54813 | Lonza Reliant GELS (2%SKG,TBE, EB,2x12W)20 PK |
| 54820 | Lonza Reliant GELS (1%SKG,TBE, EB,12W) 20 PK |
| 54821 | Lonza Reliant GELS (1%SKG,TAE, EB,12W) 20 PK |
| 54823 | Lonza Reliant Gels (NUS 3:1+,TBE,EB,12W) 20 PK |
| 54825 | Lonza Reliant GELS (2%SKG,TBE, EB,12W) 20 PK |
| 54903 | Lonza Reliant GELS (1%SKG,TBE, EB,8W) 20PK |
| 54905 | Lonza Reliant GELS (1%SKG,TBE, EB,24W) 20PK |
| 54948 | Lonza Reliant GELS (1.25%SKG,M OPS,20W) 20 PK |
| 56007 | Lonza IsoGel AGAROSE IEF Blott Paper, 250 sht. |
| 56010 | Lonza IsoGel IEF ACCESS KIT 100 WICKS/100 BLOT |
| 56014 | Lonza Precast IsoGel 等电聚焦板配件包 |
| 56015 | Lonza Precast IsoGel 等电聚焦电泳板，pH3-10 |
| 56018 | Lonza Precast IsoGel 等电聚焦电泳板，pH6-10.5 |
| 56024 | Lonza IsoGel AGAROSE IEF PLATE S PH 7-11 6PK |
| 56989 | Lonza Truband Midi HT Gel Anchor 10X15 |
| 56991 | Lonza Truband Gel Anchor 24×14 |
| 56992 | Lonza Truband BR Gel Anchor 24×20 |
| 57230 | Lonza LATITUDE (10X15,1%GEL,TBE ,EB,40W) 8PK |
| 57231 | Lonza LATITUDE (10X15,2%GEL,TBE ,EB,40W) 8PK |
| 57235 | Lonza LATITUDE (24X14,4%GEL,TBE ,EB,200W)5PK |
| 57239 | Lonza LATITUDE (10X15,2%GEL,TBE ,EB,15W) 8PK |
| 57279 | Lonza LATIT 24X14,2.5%NUS H RES,TBE,100W)5PK |
标签：lonza
Lonza内毒素检测试剂盒,Lonza代理,E50-640,N183-06,N194-03,N288-25
| 产品货号 | 产品名称 |
| E50-640 | Lonza ENDOTOXIN FOR QCL-1000 1 VIAL |
| E50-643 | Lonza Endotoxin, E. coli O55:B5 for K-QCL |
| E50-643L | Lonza 内毒素标准品, E. Coli O55:B5 |
| E700 | Lonza 美国药典内毒素标准品 |
| F245U-06 | Lonza PYROGENT BULK KIT 0.06 EU/ML SENSITIVITY |
| F245U-125 | Lonza PYROGENT BULK KIT 0.125 EU/ML SENSITIVE |
| K50-643L | Lonza Kinentic-QCL动态显色法内毒素检测试剂盒 |
| K50-643U | Lonza Kinetic-QCL Bulk Kit 100 Vials/Kit |
| LNOCODE | Lonza WinKQCL解锁码，有效期1年 |
| N183-06 | Lonza PYROGENT凝胶法鲎试剂检测试剂盒,80test，0.06EU/ml |
| N183-125 | Lonza PYROGENT凝胶法鲎试剂检测试剂盒,80test，0.125EU/ml |
| N184-25 | Lonza PYROGENT凝胶法鲎试剂检测试剂盒,250test，0.25EU/ml |
| N186 | Lonza 内毒素标准品Strain 055:B5，5*10ng/管 |
| N188 | Lonza PYROSPERSE分散剂 |
| N190 | Lonza Beta-G-封闭液试剂盒，5x5ml瓶 |
| N194-03 | Lonza PYROGENT凝胶法鲎试剂检测试剂盒,250test，0.03EU/ml |
| N194-06 | Lonza PYROGENT凝胶法鲎试剂检测试剂盒,250test，0.06EU/ml |
| N194-125 | Lonza PYROGENT凝胶法鲎试剂检测试剂盒,250test，0.125EU/ml |
| N201 | Lonza 无热源检测管，10x75mm，带螺旋盖 |
| N205 | Lonza 无热源检测管，10x75mm，无盖 |
| N207 | Lonza 无热源稀释管，13x100mm，无盖 |
| N283-06 | Lonza PYROGENT Plus凝胶法鲎试剂检测试剂盒,0.06EU/ml |
| N283-125 | Lonza PYROGENT Plus凝胶法鲎试剂检测试剂盒,0.125EU/ml |
| N284-25 | Lonza PYROGENT Plus凝胶法鲎试剂检测试剂盒,0.25EU/ml |
| N288-25 | Lonza PYROGENT Plus 4000 Tests 0.25 EU/ml Sens |
| N294-03 | Lonza PYROGENT Plus凝胶法鲎试剂检测试剂盒(含对照),200test，0.03EU/ml |
| N294-06 | Lonza PYROGENT Plus凝胶法鲎试剂检测试剂盒(含对照),200test，0.06EU/ml |
| N294-125 | Lonza PYROGENT Plus凝胶法鲎试剂检测试剂盒(含对照),200test，0.125EU/ml |
| N383 | Lonza PYROGENT-5000动态浊度法鲎试剂检测试剂盒,0.01-100EU/ml |
| N384 | Lonza PYROGENT-5000动态浊度法检测试剂盒,0.01-100EU/ml |
| N494-03 | Lonza PYROGENT Plus 4000 Tests 0.03 EU/ml Sens |
| N494-06 | Lonza PYROGENT Plus 4000 Tests 0.06 EU/ml Sens |
| N494-125 | Lonza PYROGENT Plus 4000 Tests 0.125 EU/ml Sen |
| N588 | Lonza Pyrogent-5000 Bulk Kit 2250 Test Kit |
| N688 | Lonza Pyrogent-5000 Bulk Kit 4500 Test Kit |
| S50-640 | Lonza Chromogenic Substrate Vial |
| S50-641 | Lonza 10mM氯化镁溶液，LAL用 |
| S50-642 | Lonza Tris缓冲液，50mM |
| T50-300L | Lonza Pyrogent-5000 Bulk Kit 25 Vials/Kit |
| T50-300U | Lonza Pyrogent-5000 Bulk Kit 100 Vials/Kit |
| T50-600 | Lonza Pyrogent-5000 Lysate Vial 10.4 ml |
| T50-600L | Lonza Pyrogent-5000 Bulk Kit 25 Vials/Kit |
| T50-600U | Lonza Pyrogent-5000 Bulk Kit 100 Vials/Kit |
| W50-100 | Lonza 内毒素检测用水,<0.005EU/ml |
| W50-1000 | Lonza 内毒素检测用水,<0.005EU/ml |
| W50-500 | Lonza 内毒素检测用水,<0.005EU/ml |
| W50-640 | Lonza 内毒素检测用水,<0.005EU/ml |
Lonza内毒素检测试剂盒产品列表,Lonza代理,00190035,50-647U,E194U-125,25-342
| 产品货号 | 产品名称 |
| 00190035 | Lonza 加样槽，无内毒素 |
| 00192568 | Lonza Endotoxin Challenge Vials 25 vials/kit |
| 00193783 | Lonza 内毒素 |
| 05105 | Lonza Lamp for BioTek ELx808 reader |
| 25-339S | Lonza IQ/OQ/PQ Validation on site, labour Only |
| 25-340 | Lonza 无热源96孔酶标板 |
| 25-342 | Lonza Stepped中性密度检测板 |
| 25-361 | Lonza USB to Serial Port Converter |
| 25-415 | Lonza Eppendorf BioPur 无热原吸头，200ul |
| 25-416 | Lonza EPPENDORF BIOPUR TIPS 300 UL |
| 25-417 | Lonza Eppendorf BioPur 无热原吸头，1000ul |
| 25-443 | Lonza All-in-One PC w/ BT kybd/mouse (US Eng) |
| 25-444 | Lonza Color Laser Printer (US-Letter format) |
| 25-501 | Lonza WinKQCL 5 内毒素检测和分析软件 |
| 25-502 | Lonza WinKQCL 5 Workgroup License |
| 25-503 | Lonza WinKQCL 5 Reader License |
| 25-504 | Lonza WinKQCL 3Q认证手册 |
| 25-505 | Lonza WinKQCL 5 Validation Package |
| 25-611 | Lonza WinKQCL6 Software Package |
| 25-612 | Lonza WinKQCL 6 Workgroup License |
| 25-613 | Lonza WinKQCL 6 Reader License |
| 25-614 | Lonza WinKQCL 6 Qualification Manual |
| 50-647U | Lonza QCL-1000终点显色法鲎试剂检测试剂盒,0.1-1EU/ml |
| 50-648U | Lonza QCL-1000终点显色法鲎试剂检测试剂盒,0.1-1EU/ml |
| 50-650H | Lonza Kinetic-QCL动态显色法鲎试剂检测试剂盒 |
| 50-650NV | Lonza Kinetic-QCL动态显色法鲎试剂检测试剂盒（不含水) |
| 50-650U | Lonza Kinetic-QCL动态显色法鲎试剂检测试剂盒,0.005-50EU/ml |
| 50-658NV | Lonza PyroGene重组因子C荧光法内毒素检测试剂盒(不含水), |
| 50-658U | Lonza PyroGene重组因子C荧光法内毒素检测试剂盒,0.005-50.0EU/ml |
| 7260522 | Lonza 通用测试板 |
| 7360 | Lonza Endotoxin, E. coli O55:B5 Vial Pyrogent |
| 7360L | Lonza Endotoxin, E. coli O55:B5 25 Vials/Kit |
| 7460 | Lonza Pyrogent-5000用内毒素标准品 |
| 7460L | Lonza Endotoxin, E. coli O55:B5 25 Vials/Kit |
| 80-507L | Lonza SAMPLE VIAL, PYROGEN-FREE 25/KIT |
| 80-507U | Lonza 内毒素检测样品管 |
| 85-011-SND | Lonza SND板再认证服务 |
| 85-012-SND | Lonza SND Plate Recert. – Add Wavelengths |
| B50-300L | Lonza PYROGENT-5000 RECONSTITUTION BUFFER |
| B50-300U | Lonza PYROGENT-5000 RECONSTITUTION BUFFER |
| B50-600L | Lonza PYROGENT-5000 RECONSTITUTION BUFFER |
| B50-600U | Lonza PYROGENT-5000 RECONSTITUTION BUFFER |
| E194L-03 | Lonza PYROGENT 25 VIALS/KIT 0.03 EU/ML |
| E194L-06 | Lonza PYROGENT 25 VIALS/KIT 0.06 EU/ML |
| E194L-125 | Lonza PYROGENT 25 VIALS/KIT 0.125 EU/ML |
| E194U-03 | Lonza PYROGENT 100 VIALS/KIT 0.03 EU/ML |
| E194U-06 | Lonza PYROGENT 100 VIALS/KIT 0.06 EU/ML |
| E194U-125 | Lonza PYROGENT 100 VIALS/KIT 0.125 EU/ML |
| E209L-25 | Lonza Pyrogent Bulk Kit 0.25 EU/ml Sensitivity |
| E209U-25 | Lonza Pyrogent Bulk Kit 0.25 EU/ml Sensitivity |
Lonza常规培养基产品列表,IMDM培养基,PBS缓冲液,谷氨酰胺,胰蛋白酶
| 产品货号 | 产品名称 |
| 12-722F | Lonza IMDM培养基，含HEPES和L-谷氨酰胺 |
| 12-722Q | Lonza IMDM培养基，含HEPES和L-谷氨酰胺 |
| 12-726F | Lonza IMDM培养基，含HEPES，不含谷氨酰胺 |
| 12-726Q | Lonza IMDM培养基，含HEPES，不含谷氨酰胺 |
| 12-733F | Lonza DMEM w/4.5g/L gluc w/o L-Gln, Na Pyruvat |
| 12-736E | Lonza MEM Eagle EBSS, Maint., w/FBS+, 100 ml |
| 12-736F | Lonza MEM Eagle EBSS, Maint., w/FBS+, 500 ml |
| 12-739F | Lonza Glasgow-MEM w/ L-Glutamine 500ml |
| 12-741F | Lonza DMEM 高糖培养基，4.5 g/L葡萄糖，含谷氨酰胺，不含丙酮酸钠，含酚红，不含HEPES |
| 12-743F | Lonza UltraMEM 低蛋白低血清培养基，含硒，含L-谷氨酰胺 |
| 12-747F | Lonza UltraSaline A HEPES缓冲盐，不含酚红 |
| 12-915F | Lonza IMDM培养基，含HEPES和L-谷氨酰胺，用于杂交细胞 |
| 12-917F | Lonza DMEM高糖培养基，4.5 g/L葡萄糖，不含谷氨酰胺，含丙酮酸钠，不含酚红，不含HEPES |
| 12-918F | Lonza RPMI 1640培养基，不含谷氨酰胺，不含酚红 |
| 12-923E | Lonza NCTC109 w/EBSS,Gln hybridoma, 100 ml |
| 13-114E | Lonza MEM 非必需氨基酸溶液，10mM，100x |
| 13-115E | Lonza 丙酮酸钠溶液，100mM |
| 13-607C | Lonza MEM Eagle 混合维生素 ，100x |
| 17-160E | Lonza 胰蛋白酶 HBSS溶液(10X)，25g/L |
| 17-161E | Lonza 胰蛋白酶-Versene(EDTA)溶液，0.05% |
| 17-161F | Lonza 胰蛋白酶-Versene(EDTA)溶液，0.5g/L |
| 17-512F | Lonza DPBS缓冲液(1×)，不含钙、镁离子，不含酚红 |
| 17-512Q | Lonza DPBS缓冲液(1×)，不含钙、镁离子，不含酚红 |
| 17-513F | Lonza DPBS缓冲液(1×)，含钙、镁离子，不含酚红 |
| 17-513Q | Lonza DPBS缓冲液(1×)，含钙、镁离子，不含酚红 |
| 17-515F | Lonza DPBS缓冲液(10X)，不含钙、镁离子 |
| 17-515Q | Lonza DPBS缓冲液(10X)，不含钙、镁离子，不含酚红 |
| 17-516F | Lonza PBS缓冲液(1×)，不含钙、镁离子，不含酚红 |
| 17-516Q | Lonza PBS缓冲液(1×)，不含钙、镁离子，不含酚红 |
| 17-517Q | Lonza PBS缓冲液(10X)， 不含钙、镁离子，不含酚红 |
| 17-518L | Lonza Gentamicin 50 mg/ml 10 x 10 ml screw cap |
| 17-518Z | Lonza 硫酸庆大霉素，50mg/ml |
| 17-519L | Lonza 硫酸庆大霉素，10mg/ml |
| 17-519Z | Lonza 硫酸庆大霉素，10mg/ml |
| 17-528Z | Lonza 硫酸庆大霉素，50mg/ml |
| 17-602E | Lonza 青霉素-链霉素，10K/10K |
| 17-602F | Lonza 青霉素-链霉素，10K/10K |
| 17-603E | Lonza 青霉素-链霉素，5K/5K |
| 17-605C | Lonza L-谷氨酰胺，200mM |
| 17-605E | Lonza L-谷氨酰胺，200mM |
| 17-605F | Lonza L-谷氨酰胺，200mM |
| 17-613E | Lonza 碳酸氢钠溶液，7.5% |
| 17-711E | Lonza Versene (EDTA) 溶液，0.02% |
| 17-718R | Lonza 青霉素-链霉素-L-谷氨酰胺，25K/25K/200mM |
| 17-724F | Lonza 细胞培养用水 |
| 17-724Q | Lonza 细胞培养用水 |
| 17-737E | Lonza HEPES 缓冲液，1M |
| 17-737F | Lonza HEPES Buffer,1M in normal saline,500ml |
| 17-745E | Lonza 青霉素-链霉素-两性霉素B，10K/10K/25ug |
| 17-745H | Lonza 青霉素-链霉素-两性霉素B，10K/10K/25ug |
| 17-829E | Lonza 淋巴细胞分离液 |
| 17-829F | Lonza 淋巴细胞分离液 |
| 17-836E | Lonza 两性霉素B，250 ug/ml |
| 17-836R | Lonza 两性霉素B，250 ug/ml |
| 17-838Z | Lonza ITS添加剂，500X |
| 17-839Z | Lonza ITES添加剂，500X |
| 17-905C | Lonza L-Glutamine 200mM Hybridoma test, 50 ml |
| 17-942E | Lonza 台盼蓝溶液，0.4% |
| BE02-019F | Lonza William’s Med E w/o PR, L-Gln 500ML |
| BE12-702F | Lonza RPMI 1640培养基 |
发光检测试剂盒产品列表,Lonza代理
| 产品货号 | 产品名称 |
| LT07-117 | Lonza ToxiLight 细胞毒性检测试剂盒(LT27-061*5+LT27-066*2) |
| LT07-121 | Lonza ViaLight Plus 细胞增殖及毒性检测试剂盒 |
| LT07-122 | Lonza VIALIGHT MDA PLUS-1000 TE ST |
| LT07-217 | Lonza ToxiLight 细胞毒性检测试剂盒(LT27-060*5+LT27-066*1) |
| LT07-221 | Lonza ViaLight Plus 细胞增殖及毒性检测试剂盒 |
| LT07-321 | Lonza VIALIGHT PLUS 100 PLATE |
| LT07-517 | Lonza TOXILIGHT 100% 裂解对照试剂盒 |
| LT07-600 | Lonza PDELight HTS cAMP磷酸二酯酶检测试剂盒(LT27-250+LT27-253+LT27-256) |
| LT07-610 | Lonza PPiLight 无机焦磷酸盐检测试剂盒(LT27-263+LT27-260+LT27-266) |
| LT17-217 | Lonza ToxiLight 细胞毒性检测试剂盒含96孔白板(LT27-060*5+LT27-066*1+LT27-100*5) |
| LT17-221 | Lonza VIALIGHT PLUS 5 PLATE KIT W/PLATES |
| LT27-008 | Lonza ATP标准液 |
| LT27-076 | Lonza CELL LYSIS REAGENT-50ML |
| LT27-077 | Lonza VIALIGHT PLUS CELL LYSIS REAGENT 100ML |
| LT27-102 | Lonza 底透白壁组织培养板 |
Lonza UltraCULTURE无血清培养基产品列表,Lonza代理
| 产品货号 | 产品名称 |
| 12-725F | Lonza UltraCULTURE 通用无血清培养基，不含L-谷氨酰胺 |
| BEBP12-725F | Lonza UltraCULTURE 通用无血清培养基，GMP级别 |
LONZA 12-725F UItraCULTUREW Serum-free Medium产品介绍：
LONZA 12-725F UItraCULTUREM无血清培养基是一个完全、通用的培养基，设计用于各种粘附型和非粘附型哺乳动物细胞的培养。
UltraCULTUREM无血清培养基可用于细胞生成过程中促进细胞融合，促进单核细胞、巨噬细胞、上皮细胞和成纤纤细胞系的增殖生长，并且有助于疫苗生产中病毒颗粒的产生。培养基中加入了重组人胰岛素，牛转铁蛋白和牛血清蛋白纯化混合物。
UltraCULTUREM培养基总蛋白浓度接近3mg/ml。UltraCULTURE“培养基可以作为防冷冻培养基（Cat.No.12-132A）补充物便于冻存细胞在无血清环境中培养。LONZA 12-725F UItraCULTUREM无血清培养基不含L-glutamine（L-谷氨酸盐）；用之前添加5ml 200mM/L L-谷氨酸盐溶液（Cat.Nos.17-605or 17-905）。
LONZA UItraCULTUREM Serum-free Medium产品性能及应用：
用于粘附和非粘附性哺乳动物细胞培养
疫苗产品中病毒颗粒的生成
UItraCULTURETM培养基中可能会出现混浊，但不影响产品性能
2b核转试剂盒产品列表-3,Lonza代理
| 产品货号 | 产品名称 |
| VPA-1010 | Lonza 小鼠B细胞核转染试剂盒 |
| VPA-1011 | Lonza 小鼠树突状细胞转染试剂盒 |
| VPB-1001 | Lonza HCAEC Nucleofector Kit |
| VPB-1002 | Lonza 人脐静脉内皮细胞核转染试剂盒 |
| VPB-1003 | Lonza HMVEC-L Nucleofector Kit |
| VPB-1492 | Lonza HUVEC OLD Nucleofector Kit |
| VPC-1001 | Lonza Human AoSMC Nucleofector Kit |
| VPD-1001 | Lonza 人真皮成纤维核转试剂盒 |
| VPD-1002 | Lonza Human Keratinocyte Nucleofector Kit |
| VPD-1003 | Lonza NHEM-Neo Nucleofector Kit |
| VPD-1004 | Lonza 鼠胚胎成纤维细胞转染试剂盒 |
| VPD-1005 | Lonza 小鼠胚胎成纤维细胞2核转染试剂盒 |
| VPD-1006 | Lonza MEF鼠胚胎成纤维细胞starter核转染试剂盒 |
| VPE-1001 | Lonza 人间质干细胞核转染试剂盒 |
| VPE-1002 | Lonza Rat Cardiomyocyte Neo Nucleofector Kit |
| VPF-1001 | Lonza Human Chondrocyte Nucleofector Kit |
| VPG-1001 | Lonza 小鼠神经元核转染试剂盒 |
| VPG-1002 | Lonza Chicken Neuron Nucleofector Kit |
| VPG-1003 | Lonza 大鼠神经元核转染试剂盒 |
| VPG-1004 | Lonza 小鼠神经干细胞核转染试剂盒 |
| VPG-1005 | Lonza Rat Neural Stem Cell Nucleofector Kit |
| VPH-1001 | Lonza 小鼠ES细胞核转试剂盒 |
| VPH-5002 | Lonza 人干细胞核转染启动试剂盒 |
| VPH-5012 | Lonza 人ES细胞转染试剂盒 |
| VPH-5022 | Lonza 人ES细胞转染试剂盒2 |
| VPI-1001 | Lonza 原代内皮细胞转染试剂盒 |
| VPI-1002 | Lonza 原代成纤维细胞转染试剂盒 |
| VPI-1003 | Lonza 基础原代神经细胞核转染试剂盒 |
| VPI-1004 | Lonza 基础原代平滑肌细胞核转染试剂盒 |
| VPI-1005 | Lonza 原代上皮细胞核转染试剂盒 |
| VPI-1006 | Lonza 基础神经胶质细胞核转染试剂盒 |
| VPK-1002 | Lonza 人乳腺上皮细胞核转试剂盒 |
| VPL-1004 | Lonza 鼠肝细胞核转染试剂盒 |
| VSPI-1003 | Lonza 神经元核转染试剂盒 |
| VVCA-1001 | Lonza 细胞系核转染试剂盒R（VCA-1001*4) |
| VVCA-1002 | Lonza 细胞系核转染试剂盒T（VCA-1002*4) |
| VVCA-1003 | Lonza 细胞系核转染试剂盒V（VCA-1003*4） |
| VVCA-1004 | Lonza 100 RCT Cell Line Kit C |
| VVCA-1005 | Lonza 100 RCT Cell Line Kit L |
| VVMI-1011 | Lonza 100 RCT Basic Parasite Kit 1 |
| VVMI-1021 | Lonza 100 RCT Basic Parasite Kit 2 |
| VVPA-1001 | Lonza 100 RCT Human B Cell Kit |
| VVPA-1002 | Lonza 人T细胞核转染试剂盒（VPA-1002*4） |
| VVPA-1003 | Lonza 人CD34造血祖细胞核转染试剂盒（VPA-1003*4） |
| VVPA-1004 | Lonza 100 RCT Human Dendritic Cell Kit |
| VVPA-1005 | Lonza 100 RCT Human NK Cell Kit |
| VVPA-1006 | Lonza 小鼠T细胞核转试剂盒（VPA-1006*4） |
| VVPA-1007 | Lonza 100 RCT Human Monocyte Kit |
| VVPA-1008 | Lonza 人巨噬细胞核转染试剂盒（VPA-1008*4） |
| VVPA-1009 | Lonza 小鼠巨噬细胞核转染试剂盒（VPA-1009*4） |
| VVPA-1010 | Lonza 100 RCT Mouse B Cell Kit |
| VVPA-1011 | Lonza 100 RCT Mouse Dendritic Cell Kit |
| VVPB-1001 | Lonza 100 RCT HCAEC Kit |
| VVPB-1002 | Lonza 100 RCT HUVEC Kit |
| VVPB-1003 | Lonza 100 RCT HMVEC-L Kit |
| VVPB-1492 | Lonza 100 RCT HUVEC OLD Kit |
| VVPC-1001 | Lonza 100 RCT Human AoSMC Kit |
| VVPD-1001 | Lonza 100 RCT NHDF Kit |
| VVPD-1002 | Lonza 100 RCT Human Keratinocyte Kit |
| VVPD-1003 | Lonza 100 RCT NHEM-Neo Kit |
| VVPD-1004 | Lonza 100 RCT MEF 1 Kit |
| VVPD-1005 | Lonza 100 RCT MEF 2 Kit |
| VVPE-1001 | Lonza 人间质干细胞核转染试剂盒（VPE-1001*4） |
| VVPE-1002 | Lonza 100 RCT Rat Cardiomyocyte Neo Kit |
| VVPF-1001 | Lonza 100 RCT Human Chondrocyte Kit |
| VVPG-1001 | Lonza 小鼠神经元核转染试剂盒（VPG-1001*4） |
| VVPG-1002 | Lonza 100 RCT Chicken Neuron Kit |
| VVPG-1003 | Lonza 100 RCT Rat Neuron Kit |
| VVPG-1004 | Lonza 小鼠神经干细胞核转染试剂盒（VPG-1004*4) |
| VVPG-1005 | Lonza 100 RCT Rat Neural Stem Cell Kit |
| VVPH-1001 | Lonza 鼠胚胎干细胞核转染试剂盒（VPH-1001*4） |
| VVPH-5012 | Lonza 100 RCT Human Stem Cell Kit 1 |
| VVPH-5022 | Lonza 人干细胞转染试剂盒2（VPH-5022*4） |
| VVPI-1001 | Lonza 100 RCT Basic Endothelial Cells Kit |
| VVPI-1002 | Lonza 基础成纤维细胞转染试剂盒(VPI-1002*4） |
| VVPI-1003 | Lonza 100 RCT Basic Primary Neurons Kit |
| VVPI-1004 | Lonza 100 RCT Basic SMC Kit |
| VVPI-1005 | Lonza 原代上皮细胞核转染试剂盒(VPI-1005) |
| VVPI-1006 | Lonza 100 RCT Basic Glial Cells Kit |
| VVPK-1002 | Lonza 100 RCT HMEC Kit |
| VVPL-1004 | Lonza 100 RCT Mouse/Rat Hepatocyte Kit |
| VVSI-1003 | Lonza 100 RCT Basic Neuron SCN Kit |
| VZB-1001 | Lonza 小鼠T细胞核转培养基 |
384孔核转试剂盒产品列表,lonza代理,V5SC-1002,V5SP-3010,V5SP-9001
384孔核转试剂盒产品列表 |
||
|---|---|---|
| 产品货号 | 产品名称 | 产品规格 |
| V5SC-1002 | Lonza SE Cell Line 384-well Kit | (2 PT) |
| V5SC-1010 | Lonza SE Cell Line 384-well Kit | (10 PT) |
| V5SC-2002 | Lonza SF Cell Line 384-well Kit | (2 PT) |
| V5SC-2010 | Lonza SF Cell Line 384-well Kit | (10 PT) |
| V5SC-3002 | Lonza SG Cell Line 384-well Kit | (2 PT) |
| V5SC-3010 | Lonza SG Cell Line 384-well Kit | (10 PT) |
| V5SC-9001 | Lonza Cell Line Optimization 384-well Kit | |
| V5SP-1002 | Lonza P1 Primary Cell 384-well Kit | (2 PT) |
| V5SP-1010 | Lonza P1 Primary Cell 384-well Kit | (10 PT) |
| V5SP-2002 | Lonza P2 Primary Cell 384-well Kit | (2 PT) |
| V5SP-2010 | Lonza P2 Primary Cell 384-well Kit | (10 PT) |
| V5SP-3002 | Lonza P3 Primary Cell 384-well Kit | (2 PT) |
| V5SP-3010 | Lonza P3 Primary Cell 384-well Kit | (10 PT) |
| V5SP-4002 | Lonza P4 Primary Cell 384-well Kit | (2 PT) |
| V5SP-4010 | Lonza P4 Primary Cell 384-well Kit | (10 PT) |
| V5SP-5002 | Lonza P5 Primary Cell 384-well Kit | (2 PT) |
| V5SP-5010 | Lonza P5 Primary Cell 384-well Kit | (10 PT) |
| V5SP-9001 | Lonza Primary Cell Optimiz. 384-well Kit | (1PT) |
Lonza FlashGel闪胶系统,快速进行DNA和RNA电泳分析、回收产物、不需要紫外下操作的便捷电泳系统
Lonza FlashGel闪胶系统，包含浸泡在电泳缓冲液中的预制胶、内含发光染料的上样缓冲液loading dye、特制高压电泳槽、特制非紫外光凝胶图像采集系统等，是一种快速进行DNA和RNA电泳分析、回收产物、不需要紫外下操作的便捷电泳系统。
| LONZA货号 | 英文名称 | 中文名称 |
| 50462 | FlashGel Loading Dye 5 x 1mL, 5X conc | FlashGel 上样染料(50463*5)，5X |
| 50472 | FlashGel DNA Marker 100 – 4000bp (150uL) | |
| 50473 | FlashGel DNA Marker 100bp -4kb) 500ul) | 闪胶DNA Marker, 100 bp – 4 kb 1.2% |
| 50475 | FlashGel DNA QuantLadd 100-1500bp 250uL | 闪胶QuantLadder, 100 bp (3 ng) – 1.5 kb (30 ng) |
| 50577 | FlashGel RNA Marker 0.5 kb – 9 kb | |
| 57022 | FlashGel Recovery 2.2%, 8+1 well double | |
| 57023 | FlashGel DNA Cass 1.2%,12+1well,sin tier | FlashGel DNA预制胶，1.2%琼脂糖，12+1单排孔 |
| 57024 | FlashGel RNA Kit | |
| 57025 | FlashGel Dock System | FlashGel 闪胶槽 |
| 57026 | FlashGel DNA Starter Kit | FlashGel DNA电泳启动盒(50463+50472+57023+57025) |
| 57027 | FlashGel RNA Cass 1.2%,12+1well,sin tier | FlashGel RNA预制胶，1.2%，12+1单排孔 |
| 57028 | FlashGel RNA Cass 1.2%,16+1well,dbl tier | FlashGel RNA预制胶，1.2%，16+1双排孔 |
| 57029 | FlashGel DNA Cass 1.2%,16+1well,dbl tier | FlashGel DNA预制胶，1.2%，16+1双排孔 |
| 57031 | FlashGel DNA Cass 2.2%,12+1well,sin tier | FlashGel DNA预制胶，2.2%，12+1单排孔 |
| 57032 | FlashGel DNA Cass 2.2%,16+1well,dbl tier | FlashGel DNA预制胶，2.2%，16+1双排孔 |
| 57033 | FlashGel DNA Marker 50bp -1.5 kb (500uL) | FlashGel DNA 标尺，50bp-1.5kb |
| 57034 | FlashGel DNA Marker 100bp -3kb, 500ul | |
| 57040 | FlashGel Camera | FlashGel 成像相机 |
| 57050 | FlashGel Recovery Kit | |
| 57051 | FlashGel DNA Cass 1.2%,8+1well,dbl tier | FlashGel 回收预制胶，1.2%，8+1双排孔 |
| 57060 | FlashGel Recovery Buffer | FlashGel 回收缓冲液 |
| 57061 | FlashGel Visualization Glasses | |
| 57062 | FlashGel Device Pack | FlashGel 闪胶系统(57025+57040+57068) |
| 57063 | FlashGel DNA Kit | FlashGel DNA电泳试剂盒(57023+50473+50462） |
| 57064 | FlashGel Recovery Kit | FlashGel 回收试剂盒(57051+50475+57060+50463+57061+57020) |
| 57067 | FlashGel System | FlashGel 闪胶系统(50472+57023+57040+50463+57025) |
| 57068 | FlashGel power supply | FlashGel 闪胶系统电源 |
| 57069 | FlashGel Power Supply Pack |
LONZA人原代细胞培养基,LONZA代理,CC-4519,PT-7009,CC-4175,PT-3927
Lonza人原代细胞培养基，基于不同组织来源人原代细胞体外培养需求，结合基础培养基和细胞特异性生长因子——SingleQuots专利组分，解决了原代细胞体外培养的困难，促进增殖，并保持细胞正常功能、减少变异，提高原代培养效率，通过全球实验室多年验证，获得了研究者的广泛认可。
| LONZA货号 | 英文名称 | 中文名称 |
| CC-4519 | 5-Bromo-2’deoxyuridine | 5-Bromo-2’deoxyuridine |
| CC-3187 | ABM Basal Medium 500 ml | ABM人星形细胞基础培养基 |
| PT-3102B | ADIPOGENIC INDUCTION MEDIUM | |
| PT-3102A | ADIPOGENIC MAINTENANCE MEDIUM | |
| 00193339 | AdipoLyze Lipolysis Detection Kit | |
| PT-7009 | AdipoRed Assay Reagent 5 vials | AdipoRed成脂检测试剂 |
| PT-3273 | ADSC Basal Medium, 500 ml | ADSC脂肪来源干细胞培养基基础培养基 |
| PT-4503 | ADSC-GM SQ Kit | ADSC脂肪来源干细胞培养基补充因子套装 |
| 00192627B | Adult Keratinocyte Cell Culture Kit | |
| CC-3186 | AGM BulletKit (CC-3187 & CC-4123) | AGM人星形细胞培养基(CC-3187+CC-4123) |
| CC-4123 | AGM SingleQuotKit Suppl & Growth Factors | AGM SingleQuotKit Suppl & Growth Factors |
| CC-4398 | Ascorbic Acid 0.5 ml | 抗坏血酸 |
| 00193514 | B-ALI BK: (00193515, 00193516, 00193517) | B-ALI支气管细胞气液两相培养基(00193515+00193516+00193517) |
| 00193517 | B-ALI-Differentiation Basal Medium | |
| 00193516 | B-ALI-Growth Basal Medium | |
| 00193515 | B-ALI-Medium SingleQuot Kit | |
| CC-4092 | BBE – BOVINE BRAIN EXTRACT 3 MG/ML, 2 ML | BBE – BOVINE BRAIN EXTRACT 3 MG/ML, 2 ML |
| CC-3171 | BEBM Basal Medium 500 ml | BEBM支气管细胞基础培养基 |
| CC-3170 | BEGM BulletKit (CC-3171 & CC-4175) | BEGM支气管细胞培养基(CC-3171+CC-4175) |
| CC-4175 | BEGM SingleQuot Kit Suppl&Growth Factors | BEGM SingleQuot添加剂及生长因子 |
| CC-4098 | Bovine Brain Ext. (BBE) 5 ml | 牛脑提取物 |
| CC-4009 | Bovine Pit. Extract (BPE) 2 ml | 牛垂体提取物 |
| PT-4505 | Bulletkit ADSC: Send PT-4503 & PT-3273 | ADSC人脂肪干细胞培养基(PT-4503+PT-3273) |
| CC-4455 | Bulletkit KGM-CD EA CC-3255 & CC-4456 | KGM-CD角化细胞培养基(CC-3255+CC-4456) |
| CC-4461 | Bulletkit: PNGM EA CC-3256 & CC-4462 | PNGM鼠神经细胞培养基(CC-4462+CC-3256) |
| CC-4202 | Calcium Chloride 300 mM 2 ml | 氯化钙，300 mM |
| CC-3235 | Calcium Chloride Solution 500 ml | |
| CC-3217 | CBM Basal Medium 500 ml | CBM软骨细胞基础培养基 |
| CC-3226 | CDM Basal Medium 250 ml | CDM Basal Medium 250 ml |
| CC-3225 | CDM BulletKit (CC-3226 & CC-4408) | CDM软骨细胞分化培养基(CC-3226+CC-4408) |
| CC-3216 | CGM BulletKit (CC-3217 & CC-4409) | CGM软骨细胞生长培养基(CC-3217+CC-4409) |
| CC-4408 | Chondrocyte Diff. Medium SingleQuots | Chondrocyte Diff. Medium SingleQuots |
| CC-4409 | Chondrocyte Growth Med. SingleQuots | CGM SingleQuots 添加剂及生长因子 |
| CC-3233 | Chondrocyte ReagentPack | ReagentPack软骨细胞传代试剂 |
| CC-3251 | Corneal Mod Basal Medium 250 ml | Corneal Mod Basal Medium 250 ml |
| CC-4443 | Corneal Model Differentiation Kit | Corneal Model Differentiation Kit |
| MCST250 | Cryo human Stellate cell growth media | 人星状细胞生长培养基 |
| MCAT50 | Cryo NR Animal Hpctye Thawing Medium | 非啮齿类动物肝脏细胞融化培养基 |
| PT-3925 | DIFFERENTIATION BASAL MED – CHONDROGENIC | DIFFERENTIATION BASAL MED – CHONDROGENIC |
| PT-3924 | DIFFERENTIATION BASAL MEDIUM- OSTEOGENIC | DIFFERENTIATION BASAL MEDIUM- OSTEOGENIC |
| PT-3927 | DPSC Basal Medium 500 ml | DPSC人牙髓干细胞基础培养基l |
| PT-3005 | DPSC BulletKit (PT-3927&PT-4516) | 牙髓间充质干细胞培养基(PT-3927+PT-4516) |
| PT-4516 | DPSC SingleQuot Kit | DPSC牙髓干细胞培养基补充因子套装 |
| PT-4515 | EasyDiff Multipack-Erythroid (3xPT-4514) | |
| PT-4511 | EasyDiff Multipack-Myeloid (3 x PT-4510) | |
| PT-4513 | EasyDiff Multipk-MK (3xPT-4512) | |
| PT-4514 | EasyDiff™ Supplement – Erythroid | |
| PT-4512 | EasyDiff™ Supplement – Megakaryocyte | |
| PT-4510 | EasyDiff™ Supplement – Myeloid | |
| CC-3121 | EBM Basal Medium 500 ml | EBM内皮细胞基础培养基 |
| CC-3129 | EBM Basal Medium Phenol Red Free, 500 ml | EBM内皮细胞基础培养基，无酚红 |
| CC-3156 | EBM-2 Basal Medium 500 ml | EBM-2内皮细胞基础培养基 |
| CC-5036 | EBM-Plus Basal Medium 475 ml | |
| CC-3124 | EGM BulletKit (CC-3121 & CC-4133) | EGM内皮细胞培养基套装(CC-3121+CC-4133) |
| CC-3024 | EGM Complete Medium 500 ml | EGM内皮细胞完全培养基套装(CC-3024A+CC-4092) |
| CC-4133 | EGM SingleQuot Kit Suppl&Growth Factors | EGM SingleQuot 添加剂及生长因子 |
| CC-3162 | EGM-2 BulletKit (CC-3156 & CC-4176) | EGM-2血管内皮细胞培养基(CC-3156+CC-4176) |
| CC-3202 | EGM-2 MV BulletKit (CC-3156 & CC-4147) | EGM-2 MV微血管内皮细胞培养基(CC-3156+CC-4147) |
| CC-4147 | EGM-2 MV SingleQuot Suppl&Growth Factors | EGM-2 MV SingleQuot添加剂及生长因子 |
| CC-4176 | EGM-2 SingleQuot Kit Suppl&Growth Factor | EGM-2 SingleQuot 添加剂及生长因子 |
| CC-3125 | EGM-MV BulletKit (CC-3121 & CC-4143) | EGM-MV微血管细胞培养基套装(CC-3121+CC-4143) |
| CC-4143 | EGM-MV SingleQuot Suppl&Growth Factors | EGM-MV SingleQuot Suppl&Growth Factors |
| CC-5035 | EGM-Plus BK (CC-4542+CC-5036) | EGM-Plus 内皮细胞培养基(CC-4542+CC-5036) |
| CC-4542 | EGM-Plus SingleQuot Kit | |
| 00190860 | Endothelial Basal Medium -2 (EBM-2), 1L | EBM-2内皮细胞基础培养基 |
| CC-4510 | Endothelin 3 (ET3) Lyophilized | 内皮素-3（ET-3）生长因子 |
| CC-3131 | FBM Fibroblast Basal Medium, 500 ml | FBM 成纤维细胞基础培养基 |
| CC-3130 | FGM BulletKit (CC-3131 & CC-4134) | FGM成纤维细胞培养基套装(CC-3131+CC-4134) |
| CC-4134 | FGM SingleQuot Kit Suppl&Growth Factors | FGM SingleQuot Kit Suppl&Growth Factors |
| CC-3132 | FGM-2 BulletKit (CC-3131 & CC-4126) | FGM-2成纤维细胞培养基(CC-3131+CC-4126) |
| CC-4126 | FGM-2 SingleQuotKit Suppl&Growth Factors | FGM-2 成纤维培养基补充因子套装 |
| CC-4526 | FGM-3 BulletKit (CC-3131 & CC-4525) | FGM-3心肌成纤维细胞生长培养基(CC-3131+CC-4525) |
| CC-4525 | FGM-3 SingleQuots Kit | FGM-3 SingleQuots Kit |
| 00199041 | FGM-CD BulletKit (00199019, 00199020) | FGM-CD真皮成纤维细胞培养基(00199019+00199020) |
| CC-4083 | GA-1000 5.0 ML | GA-1000（庆大霉素，两性霉素） |
| CC-3199 | HBM Basal Medium 500 ml | HBM人肝细胞基础培养基 |
| CC-3198 | HCM BulletKit (CC-3199 & CC-4182) | HCM人肝细胞培养基(CC-3199+CC-4182) |
| CC-4182 | HCM SingleQuot Kit | HCM SingleQuot 添加剂和生长因子 |
| CC-4107 | hEGF Epidermal Growth Factor, 0.5 ml | hEGF人表皮生长因子 |
| MH100 | HepaRG Base Medium w/ Supplement | HepaRG Base Medium w/ Supplement |
| ADD721 | HepaRG Differentiation Medium Suppl | |
| ADD711 | HepaRG Growth medium Supplement. | |
| MHIND | HepaRG Induction (serum-free) Medium | HepaRG Induction (serum-free) Medium |
| MHMET | HepaRG Maintenance/Metabolism Supplement | HepaRG Maintenance/Metabolism Supplement |
| MHPIT | HepaRG Pre-Induction and Tox Medium | HepaRG预诱导和毒性培养基补充物 |
| MM250 | Hepatocyte Maintenance Media w/ Splmnt | 肝脏细胞维持培养基 |
| MP250 | Hepatocyte Plating Media w/ Supplement | Hepatocyte Plating Media w/ Supplement |
| MP100 | Hepatocyte Plating Medium w/ Supplement | 肝实质细胞铺板培养基(MP100-1+MP100-2) |
| CC-5022 | HEPES Buffered Saline Solution, 100 ml | HEPES缓冲盐溶液 |
| CC-5024 | HEPES Buffered Saline Solution, 500 ml | HEPES缓冲盐溶液 |
| CC-4068 | hFGF Fibroblastic Growth Factor, 1 ml | hFGF Fibroblastic Growth Factor, 1 ml |
| CC-3197 | HMM Maintenance Medium 500 ml | HBM 人肝细胞维持培养基基础培养基 |
| CC-4192 | HMM SingleQuot Kit Hepat. Maint. Medium | HMM SingleQuot 添加剂和生长因子 |
| PT-4135 | HMSC Adipogenic Induction SQ Kit | |
| PT-4122 | HMSC Adipogenic Maintenance SQ Kit | hMSC Adipogenic Maintenance SQ Kit |
| PT-3004 | hMSC Adipogenic PT3102B,3102A,4122,4135 | hMSC人间质干细胞脂肪分化培养基(PT3102B+3102A+4122+4135) |
| PT-3003 | HMSC Chondro BulletKit PT-3925 & PT-4121 | hMSC人间质干细胞软骨分化培养基(PT-3925+PT-4121) |
| PT-3002 | HMSC Osteogenic BulletKit PT-3924/4120 | hMSC人间质干细胞成骨分化培养基(PT-3924+PT-4120) |
| PT-4121 | HMSC SQ KIT – CHONDRO | HMSC SQ KIT – CHONDRO |
| PT-4120 | HMSC SQ KIT – OSTEO | hMSC 成骨分化SingleQuots添加剂及生长因子 |
| PT-3926 | HPGM Hematopoietic Growth Medium, 500 ml | HPGM造血细胞无血清培养基 |
| CC-4205 | Human Transferrin 0.5 ml | Human Transferrin 0.5 ml |
| CC-3255 | KBM – CD Basal Media 500 ml | KBM – CD Basal Media 500 ml |
| CC-3101 | KBM Basal Medium 500 ml | KBM角化细胞基础培养基 |
| CC-3104 | KBM Basal Medium w/o Ca++, 500 ml | KBM角化细胞基础培养基，不含Ca离子 |
| CC-3103 | KBM-2 Basal Medium 500 ml | KBM-2角质形成细胞基础培养基 |
| CC-3158 | KBM-2 Basal Medium w/o Ca++, 500 ml | KBM-2 Basal Medium w/o Ca++, 500 ml |
| 00192151 | KBM-Gold Basal Medium | KBM-Gold角质细胞基础培养基 |
| 00195130 | KBM-Gold™ Basal Medium – PR&Calcium Free | |
| 00192152 | Keratinocyte Growth Medium -Gold single | KGM Gold SingleQuots添加剂及生长因子 |
| CC-3111 | KGM BulletKit (CC-3101 & CC-4131) | KGM角化细胞培养基套装(CC-3101+CC-4131) |
| CC-3112 | KGM BulletKit w/o Ca++ CC-3104 & CC-4131 | KGM角质细胞培养基套装(CC-4131+ CC-3104) |
| CC-3004 | KGM Complete Medium suppl w/o Ca++,500ml | |
| 00192060 | KGM Gold Bulletkit 00192152 & 00192151 | KGM-Gold 角质细胞培养基套装(00192151+00192152) |
| CC-4131 | KGM SingleQuot Kit Suppl&Growth Factors | KGM SingleQuot添加剂及生长因子 |
| CC-3107 | KGM-2 BulletKit (CC-3103 & CC-4152) | KGM-2角化细胞培养基套装(CC-3103+CC-4152) |
| CC-3108 | KGM-2 BulletKit w/o Ca++ CC-3158&CC-4152 | KGM-2角化细胞培养基套装(CC-3158+CC-4152)，不含Ca离子 |
| CC-4152 | KGM-2 SingleQuot Kit Suppl&Growth Factor | KGM-2 SingleQuot添加剂及生长因子 |
| CC-4456 | KGM-CD Singlequots Suppl&Growth Factors | KGM-CD Singlequots Suppl&Growth Factors |
| 00195769 | KGM-Gold CaFree BK (00195130, 00192152) | KGM-Gold 角质细胞培养基套装(00195130+00192152)，不含钙离子，不含酚红 |
| CC-3211 | LGM-3 Growth Medium 500 ml | LGM-3 淋巴细胞培养基 |
| CC-3250 | MBM-4 Basal Medium 500 ml | MBM-4 Basal Medium 500 ml |
| MCHT50 | MCHT50 – Cryo HH thawing media | 人肝细胞复苏培养基 |
| CC-3151 | MEBM Basal Medium 500 ml | MEBM 乳腺上皮细胞基础培养基 |
| CC-3152 | MEBM Basal Medium w/o NaCHO3, 500 ml | |
| CC-3153 | MEBM Basal Medium w/o Phenol Red, 500 ml | MEBM 乳腺上皮细胞基础培养基，无酚红 |
| CC-3150 | MEGM BulletKit (CC-3151 & CC-4136) | MEGM 乳腺上皮细胞培养基(CC-3151+CC-4136) |
| CC-3051 | MEGM Complete (CC-3051A & CC-4009) | MEGM 乳腺上皮细胞完全培养基套装(CC-3051A+CC-4009) |
| CC-4136 | MEGM SingleQuot Kit Suppl&Growth Factors | MEGM SingleQuot添加剂及生长因子 |
| CC-3249 | MGM-4 BulletKit (CC-3250 & CC-4435) | MGM-4 BulletKit (CC-3250 & CC-4435) |
| CC-4435 | MGM-4 SingleQuot Kit Suppl&Growth Factor | MGM-4 SingleQuot Kit Suppl&Growth Factor |
| CC-3147 | MsBM Basal Medium 500 ml | MsBM Basal Medium 500 ml |
| PT-3238 | MSCBM hMSC Basal Medium, 440 ml | MSCGM人间充质干细胞基础培养基440 ml |
| PT-3001 | MSCGM BulletKit (PT-3238 & PT-4105) | MSCGM间质干细胞培养基(PT-3238+PT-4105) |
| PT-4105 | MSCGM hMSC SingleQuot Kit | MSCGM人间充质干细胞培养基补充液及细胞因子 |
| CC-3146 | MsGM BulletKit (CC-3147 & CC-4146) | MsGM BulletKit (CC-3147 & CC-4146) |
| CC-4146 | MsGM SingleQuot Kit Suppl&Growth Factors | MsGM SingleQuot Kit Suppl&Growth Factors |
| CC-3236 | NaCl Solution 500 ml | |
| CC-4241 | Neural Prog. Maint. Med SingleQuot Kit | 神经前体细胞维持培养基因子套装 |
| CC-4242 | Neural Progenitor Suppl. SingleQuot Kit | 神经前体细胞培养基补充因子套装 |
| CC-3229 | NPDM BulletKit (CC-3210 & CC-4242) | NPDM BulletKit (CC-3210 & CC-4242) |
| CC-3209 | NPMM BulletKit (CC-3210,CC-4241&CC-4242) | NPMM神经祖细胞培养基(CC-3210+CC-4241+CC-4242) |
| CC-4323 | NSF-1 Neural Survival Factor-1, 4 ml | NSF-1因子 |
| CC-3208 | OBM Basal Medium Medium, 500 ml | OBM Basal Medium Medium, 500 ml |
| PT-8001 | OCP BulletKit (PT-8201 & PT-9501) | OCP人破骨前体细胞培养基(PT-8201+PT-9501) |
| PT-8201 | OCP Osteoclast Precur. Basal, 100 ml | OCP人破骨前体细胞基础培养基 |
| PT-9501 | OCP Osteoclast Precur. SingleQuot Kit | OCP人破骨前体细胞SingleQuot添加剂及生长因子 |
| CC-3207 | OGM BulletKit (CC-3208 & CC-4193) | OGM成骨细胞培养基(CC-3208+CC-4193) |
| CC-4194 | OGM Diff. SingleQuot Suppl&Growth Factor | OGM Diff SingleQuot 添加剂及生长因子 |
| CC-4193 | OGM SingleQuot Kit Suppl&Growth Factors | OGM SingleQuot Kit Suppl&Growth Factors |
| PA-1000 | OsteoAssay Bone Plate | OsteoAssay 人骨吸收检测板 |
| PA-1500 | OsteoLyse Assay Kit | OsteoLyse Assay Kit |
| CC-3210 | PBM Neural Progenitor Basal Med,200 ml | NPBM 神经祖细胞基础培养基 |
| PT-8200 | PBM Pre-adipocyte Basal Medium, 500 ml | |
| PT-8202 | PGM-2 Basal Medium | PGM-2 Basal Medium |
| PT-8002 | PGM-2 Bulletkit (PT-9502 & PT-8202) | 人脂肪前体细胞培养基(PT-9502+PT-8202) |
| PT-9502 | PGM-2 Singlequot Kit | PGM-2 Singlequot Kit |
| CC-4462 | PNGM Singlequot Kit | PNGM Singlequot Kit |
| CC-4512 | PNGM-A Bulletkit CC-3256/CC-4511/CC-4462 | PNGM-A Bulletkit CC-3256/CC-4511/CC-4462 |
| CC-4511 | PNGM-A SingleQuot Kit | PNGM-A SingleQuot Kit |
| MCHT50P | Pooled Human Thawing medium | 人肝脏细胞-混合供体-复苏培养基 |
| CC-3165 | PrEBM Basal Medium 500 ml | PrEBM Basal Medium 500 ml |
| CC-3166 | PrEGM BulletKit (CC-3165 & CC-4177) | PrEGM前列腺上皮细胞培养基(CC-3165+CC-4177) |
| CC-4177 | PrEGM SingleQuot Kit Suppl&Growth Factor | PrEGM SingleQuot添加剂及生长因子 |
| CC-3256 | Primary Neuron Basal Medium | PNBM神经元原代细胞基础培养基 |
| CC-3275 | Rat Cardiac Myocyte Basal Medium | Rat Cardiac Myocyte Basal Medium |
| CC-4516 | Rat Cardiac Myocyte Growth Medium SQ Kit | Rat Cardiac Myocyte Growth Medium SQ Kit |
| CC-4515 | Rat CMG Bullet Kit (CC-3275 & CC-4516) | Rat CMG Bullet Kit (CC-3275 & CC-4516) |
| MCRT50 | Rat Hpctye Thawing Medium | 鼠和猴肝细胞复苏培养基 |
| 00192855 | Rat MSC Adipogenic BulletKit | |
| 00192854 | Rat MSC Osteogenic BK (00192829&PT-3924) | |
| 00192853 | Rat MSCGM BulletKit | |
| 00192820 | Rat MSCGM SingleQuots Kit | |
| CC-5034 | ReagentPack Subculture Reagents | ReagentPack 传代试剂套装（CC-5002+CC-5012+CC-5022) |
| CC-3191 | REBM Basal Medium 500 ml | REBM人肾脏细胞基础培养基 |
| CC-3190 | REGM BulletKit (CC-3191 & CC-4127) | REGM人肾脏细胞培养基(CC-3191+CC-4127) |
| CC-4127 | REGM SingleQuot Kit Suppl&Growth Factors | REGM SingleQuot添加剂及生长因子 |
| 00192829 | rMSC Osteogenic SQ Kit | |
| 00195406 | RtEBM-Ret Pig Epi Cell Basal Medium | RtEGM视网膜色素上皮细胞基础培养基 |
| 00195409 | RtEGM Bulletkit (00195406 & 00195407) | RtEGM视网膜色素上皮细胞培养基套装(00195406 & 00195407) |
| 00195407 | RtEGM™ – SingleQuots® Kit | |
| CC-3119 | SABM Basal Medium 500 ml | SABM小气管上皮细胞基础培养基 |
| CC-3118 | SAGM BulletKit (CC-3119 & CC-4124) | SAGM小气管上皮细胞培养基套装(CC-3119+CC-4124) |
| CC-4124 | SAGM SingleQuot Kit Suppl&Growth Factors | SAGM SingleQuot添加剂及生长因子 |
| CC-4539 | S-ALI BulletKit: CC-3281/CC-3282/CC-4538 | S-ALI小气道上皮细胞培养基（CC-3281+CC-3282+CC-4538) |
| CC-3281 | S-ALI Small Airway Growth Basal Media | |
| CC-3204 | SCBM Basal Medium 500 ml | SCBM 基质细胞基础培养基 |
| CC-3205 | SCGM BulletKit (CC-3204 & CC-4181) | SCGM BulletKit (CC-3204 & CC-4181) |
| CC-4181 | SCGM SingleQuot Kit Suppl&Growth Factors | SCGM SingleQuot Kit Suppl&Growth Factors |
| 00191053 | SeGM Bulletkit (00191051 & 00191052) | |
| 00191051 | Sertoli Cell Basal Medium (SeBM) | |
| 00191052 | Sertoli Cell Growth Medium single quot | |
| CC-3161 | SkBM Basal Medium 500 ml | SkBM Basal Medium 500 ml |
| CC-3246 | SkBM-2 Basal Medium 500 ml | SkBM-2 Basal Medium 500 ml |
| CC-3160 | SkGM BulletKit (CC-3161 & CC-4139) | SkGM骨骼肌细胞培养基(CC-3161+CC-4139) |
| CC-4139 | SkGM SingleQuot Kit Suppl&Growth Factors | SkGM SingleQuot Kit Suppl&Growth Factors |
| CC-3245 | SkGM-2 BulletKit (CC-3244 & CC-3246) | SkGM-2骨骼肌细胞培养基(CC-3244+CC-3246) |
| CC-3244 | SkGM-2 SingleQuots Suppl&Growth Factors | SkGM-2 SingleQuots添加剂及生长因子 |
| CC-3181 | SmBM Basal Medium 500 ml | SmBM平滑肌细胞基础培养基 |
| CC-3182 | SmGM-2 BulletKit (CC-3181 & CC-4149) | SmGM-2平滑肌细胞培养基CC-3181+CC-4149) |
| CC-4149 | SmGM-2 SingleQuot Suppl&Growth Factors | SmGM-2 SingleQuot添加剂及生长因子 |
| CC-3237 | Sodium Citrate Solution 100 ml | |
| PT-4124 | TGF Beta 3 for HMSC Chondro diff | 人间质干细胞软骨分化TGF-β3因子 |
| MHTAP | Thaw and plating medium | HepaRG复苏和贴壁培养基补充物 |
| CC-5002 | Trypsin Neutralizing Soln 100 ml | 胰酶中和溶液 |
| CC-3232 | Trypsin/EDTA for MSC 100 ml | 胰酶/EDTA，人间充质干细胞专用 |
| CC-5012 | Trypsin/EDTA Solution 100 ml | 胰酶/EDTA溶液 |
lonza,CC-2703,原代细胞和干细胞,人外周血单个核细胞(hPBMC) CC-2703
Catalog #: CC-2703
Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 100 million cells
Product Overview
Mononuclear cell (MNC) rich cells from leukapheresis are depleted of RBCs and platelets. MNC pools contain, on average, 50% T cells, 20% monocytes, 10% B cells, 10% NK cells and may contain up to 5% granulocytes. Cells are cryopreserved in Lonza cryopreservation media. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols.
来自白细胞分离术的富含单核细胞 (MNC) 的细胞耗尽了红细胞和血小板。 MNC 池平均含有 50% 的 T 细胞、20% 的单核细胞、10% 的 B 细胞、10% 的 NK 细胞，并且可能含有高达 5% 的粒细胞。 细胞在 Lonza 冷冻保存培养基中冷冻保存。 使用 AO/PI 确定计数和活力。 按照 IRB 协议从健康供体收集细胞。
Benefits
- Test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis (CoA) is provided for each cell lot purchased
Applications
- ELISPOT
- Proliferation assays
- Immunogenicity assays
- Cell therapy process development
- Humanized mice
- Biomarker discovery
Content & Storage
Content
1 x Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 100 million cells
Instructions, SDS, CoA
lonza,原代细胞和干细胞,人外周血单个核细胞hPBMC CC-2705
Catalog #: CC-2705
Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 25 million cells
Product Overview
Mononuclear cell (MNC) rich cells from leukapheresis are depleted of RBCs and platelets. MNC pools contain, on average, 50% T cells, 20% monocytes, 10% B cells, 10% NK cells and may contain up to 5% granulocytes. Cells are cryopreserved in Lonza cryopreservation media. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols.
来自白细胞分离术的富含单核细胞 (MNC) 的细胞耗尽了红细胞和血小板。 MNC 池平均含有 50% 的 T 细胞、20% 的单核细胞、10% 的 B 细胞、10% 的 NK 细胞，并且可能含有高达 5% 的粒细胞。 细胞在 Lonza 冷冻保存培养基中冷冻保存。 使用 AO/PI 确定计数和活力。 按照 IRB 协议从健康供体收集细胞。
Benefits
- Test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis (CoA) is provided for each cell lot purchased
Applications
- ELISPOT
- Proliferation assays
- Immunogenicity assays
- Cell therapy process development
- Humanized mice
- Biomarker discovery
Content & Storage
Content
1 x Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 25 million cells
Instructions, SDS, CoA
lonza,原代细胞和干细胞,D-HBE-COPD 患病人支气管/气管上皮细胞B-ALITM 的 COPD
Catalog #: 00195275S
Product Overview
Chronic bronchitis is marked by long-term cough and excess mucous. Emphysema leads to the destruction of the lungs over time. Most people with COPD have a combination of both conditions. Usually COPD is associated with bronchial epithelium changes.
Cryopreserved diseased human bronchial epithelial cells from donors diagnosed with COPD are screened for air liquid interface differentiation. These cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Our catalog number 00195275S are suitable for air-liquid interface studies and are guaranteed to promote full differentiation of the airway epithelium expressed by the formation of a polarized epithelium with good barrier function (transepithelial resistance), secretory phenotype (mucin secretion) and ciliogenesis. Differentiation usually will occur by day 22 in culture.
Benefits
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis provided for every cell lot
Content & Storage
Content
1 x Cryopreserved ampule containing ≥ 500,000 cells
Instructions, SDS, CoA
Related Products
NHBE – Human Bronchial/Tracheal Epithelial Cells for B-ALITM Culture CC-2540S
NHBE – 用于 B-ALITM 培养的人支气管/气管上皮细胞 CC-2540S
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540
NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞
D-SAEC-COPD – Diseased Small Airway Epithelial Cells – COPD CC-2934
D-SAEC-COPD – 患病小气道上皮细胞 – COPD CC-2934
D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma 00194911
D-HBE-As – 患病的人支气管/气管上皮细胞 – 哮喘 00194911
D-HBE-CF – Diseased Human Bronchial/Tracheal Epithelial Cells – Cystic Fibrosis 00196979
D-HBE-CF – 患病的人支气管/气管上皮细胞 – 囊性纤维化 00196979
lonza ,lonza CC-2704,原代细胞和干细胞,人外周血单个核细胞(hPBMC)
Catalog #: CC-2704
Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 10 million cells
Product Overview
来自白细胞分离术的富含单核细胞 (MNC) 的细胞耗尽了红细胞和血小板。 MNC 池平均含有 50% 的 T 细胞、20% 的单核细胞、10% 的 B 细胞、10% 的 NK 细胞，并且可能含有高达 5% 的粒细胞。 细胞在 Lonza 冷冻保存培养基中冷冻保存。 使用 AO/PI 确定计数和活力。 按照 IRB 协议从健康供体收集细胞。
Benefits
- Test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis (CoA) is provided for each cell lot purchased
Applications
- ELISPOT
- Proliferation assays
- Immunogenicity assays
- Cell therapy process development
- Humanized mice
- Biomarker discovery
Content & Storage
Content
1 x Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 10 million cells
lonza,lonza CC-3162,lonza CC-3156,EGM™-2 内皮细胞生长培养基-2 BulletKit™
货号: CC-3162
Culture system containing EBMTM-2 Basal Medium (CC-3156) and EGMTM-2 SingleQuotsTM Supplements (CC-4176) required for growth of Endothelial Cells. Available for immediate shipment.
Request Information or Quote or do you need a different format or volume for this media product? Contact Lonza CellBio Services to discuss custom format and formulation options.
包含内皮细胞生长所需的 EBMTM-2 基础培养基 (CC-3156) 和 EGMTM-2 SingleQuotsTM 补充剂 (CC-4176) 的培养系统。 可立即发货。
请求信息或报价，或者您是否需要此媒体产品的不同格式或音量？ 联系 Lonza CellBio Services 讨论定制形式和配方选项。
Product Overview
Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. EGMTM – 2 Medium is suitable to grow most types of large vessel endothelial cells. Each type of endothelial cell growth medium is guaranteed to perform when used to grow Lonza’s Endothelial Primary Cells.
从多种内皮细胞生长培养基中进行选择，以轻松培养原代内皮细胞。 每种内皮细胞生长培养基都有其特殊的配方，并针对某些细胞类型进行了优化。 EGMTM – 2 培养基适合培养大多数类型的大血管内皮细胞。 每种类型的内皮细胞生长培养基在用于培养 Lonza 的内皮原代细胞时都能保证发挥作用。
The following Endothelial Cell Growth Medium BulletKitsTM are available:
- EGMTM BulletKitTM (CC-3124) – Optimized for HUVECs cat. no. CC-2517 and CC-2519, contains 2% FBS and BBE. Does not contain exogenous VEGF.
- EGMTM-Plus BulletKitTM (CC-5035) – Enhanced version of EGMTM Medium. Optimized for HUVECs cat. no. CC-2935. Contains 2% FBS and BBE. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGMTM Medium.
- EGMTM-2 BulletKitTM (CC-3162) – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Contains 2% FBS and VEGF for rapid proliferation. Does not contain BBE.
- EGMTM-2 MV BulletKitTM (CC-3202) – Coronary and Iliac artery, human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. Does not contain BBE.
Lonza guarantees the performance of endothelial cells only if appropriate EGM Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.
只有在专门使用适当的 EGM 培养基和试剂并遵循推荐的储存和使用协议时，龙沙才能保证内皮细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
Content & Storage
Content
1 x EBMTM-2 Basal Medium (CC-3156), 500 mL
1 x EGMTM-2 SingleQuotsTM Supplement Pack (CC-4176) containing:
1 x Bottle FBS, 10.00 mL
1 x Natural Cap Vial with Hydrocortisone, 0.20 mL
1 x Gray Cap Vial with hFGF-B, 2.00 mL
1 x White Cap Vial with VEGF, 0.50 mL
1 x Yellow Cap Vial with R3-IGF-1, 0.50 mL
1 x Blue Cap Vial with Ascorbic Acid, 0.50 mL
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Orange Cap Vial with Heparin, 0.50 mL
Instructions, SDS, CoA
Related Products
HUVEC-XL – Human Umbilical Vein Endothelial Cells, Large Volume, Pooled 00191027
HUVEC-XL – 人脐静脉内皮细胞，大容量，混合 00191027
D-HAEC – Diseased Human Aortic Endothelial Cells – Diabetes Type I CC-2919
D-HAEC – 患病的人主动脉内皮细胞 – I 型糖尿病 CC-2919
D-HAEC – Diseased Human Aortic Endothelial Cells – Diabetes Type II CC-2920
D-HAEC – 患病的人主动脉内皮细胞 – II 型糖尿病 CC-2920
D-HPAEC – Diseased Human Pulmonary Artery Endothelial Cells – Diabetes Type I CC-2923
D-HPAEC – 患病的人肺动脉内皮细胞 – I 型糖尿病 CC-2923
EBM™-2 Endothelial Cell Growth Basal Medium-2, 500 mL CC-3156
EBM™-2 内皮细胞生长基础培养基-2，500 mL CC-3156
D-HPAEC – Diseased Human Pulmonary Artery Endothelial Cells – Diabetes Type II CC-2924
D-HPAEC – 患病的人肺动脉内皮细胞 – II 型糖尿病 CC-2924
EGMTM-2 Endothelial SingleQuotsTM Kit CC-4176
EGMTM-2 内皮 SingleQuotsTM 套件 CC-4176
lonza,lonza CC-3202,CC-4147,EGMTM -2 MV 微血管内皮细胞生长培养基-2 BulletKitTM
货号: CC-3202
Culture system containing EBMTM-2 Basal Medium (CC-3156) and EGMTM-2 MV Microvascular Endothelial Cell Growth Medium SingleQuotsTM supplements (CC-4147) required for growth of Microvascular Endothelial Cells
包含微血管内皮细胞生长所需的 EBMTM-2 基础培养基 (CC-3156) 和 EGMTM-2 MV 微血管内皮细胞生长培养基 SingleQuotsTM 补充剂 (CC-4147) 的培养系统
Product Overview
Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used to grow CloneticsTM Primary Cells.
从多种内皮细胞生长培养基中进行选择，以轻松培养原代内皮细胞。 每种内皮细胞生长培养基都有其特殊的配方，并针对某些细胞类型进行了优化。 每种类型的内皮细胞生长培养基在用于培养 CloneticsTM 原代细胞时都能保证发挥作用。
The following Endothelial Cell Growth Medium BulletKitsTM are available:
- EGMTM-2 BulletKitTM – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Contains 2% FBS and VEGF for rapid proliferation. Does not contain BBE.
- EGMTM BulletKitTM – Optimized for HUVECs cat. no. CC-2517 and CC-2519, contains 2% FBS and BBE. Does not contain exogenous VEGF.
- EGMTM-Plus BulletKitTM – Enhanced version of EGMTMTM Medium. Optimized for HUVECs cat. no. CC-2935. Contains 2% FBS and BBE. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGMTMTM Medium.
- EGMTM-2MV BulletKitTM – Coronary and Iliac artery, human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. Does not contain BBE.
- EGMTM-2 BulletKitTM – 针对某些 HUVEC、主动脉和肺动脉内皮细胞进行了优化。 含有 2% FBS 和 VEGF 用于快速增殖。 不含 BBE。
EGMTM BulletKitTM – 针对 HUVEC 猫进行了优化。 不。 CC-2517 和 CC-2519，含有 2% FBS 和 BBE。 不含外源性VEGF。
EGMTM-Plus BulletKitTM – EGMTMTM Medium 的增强版。 针对 HUVEC 猫进行了优化。 不。 CC-2935。 含有 2% FBS 和 BBE。 不含外源性VEGF。 比在 EGMTMTM 培养基中分离的 HUVEC 增殖更快。
EGMTM-2MV BulletKitTM – 冠状动脉和髂动脉，人类微血管内皮细胞，含有 5% 的 FBS 和 VEGF，用于快速增殖。 不含 BBE。
Lonza guarantees the performance of CloneticsTM/PoieticsTM Cells only if appropriate CloneticsTM/PoieticsTM Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.
只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
Content & Storage
Content
1 x EBMTM-2 Basal Medium (CC-3156), 500 mL
1 x EGMTM-2 MV SingleQuotsTM Supplement Pack (CC-4147) containing:
1 x Bottle FBS, 25.00 mL
1 x Natural Cap Vial with Hydrocortisone, 0.20 mL
1 x Gray Cap Vial with hFGF-B, 2.00 mL
1 x White Cap Vial with VEGF, 0.50 mL
1 x Yellow Cap Vial with R3-IGF-1, 0.50 mL
1 x Blue Cap Vial with Ascorbic Acid, 0.50 mL
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 m
Safety Data Sheets (SDS)
View SDS
SDS – German
SDS – Italian
SDS – Dutch
SDS – French
SDS – Spanish
SDS – English
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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EGMTM-2 MV Microvascular Endothelial SingleQuotsTM Kit CC-4147
EGMTM-2 MV 微血管内皮 SingleQuotsTM 套件 CC-4147
EBM™-2 Endothelial Cell Growth Basal Medium-2, 500 mL CC-3156
EBM™-2 内皮细胞生长基础培养基-2，500 mL CC-3156
D-HCAEC – Diseased Human Coronary Artery Endothelial Cells – Diabetes Type I CC-2921
D-HCAEC – 患病的人冠状动脉内皮细胞 – I 型糖尿病 CC-2921
D-HCAEC – Diseased Human Coronary Artery Endothelial Cells – Diabetes Type II CC-2922
D-HCAEC – 患病的人类冠状动脉内皮细胞 – II 型糖尿病 CC-2922
D-HMVEC-C – Diseased Human Cardiac Microvascular Endothelial Cells – Diabetes Type I CC-2927
D-HMVEC-C – 患病的人类心脏微血管内皮细胞 – I 型糖尿病 CC-2927
D-HMVEC-C – Diseased Human Cardiac Microvascular Endothelial Cells – Diabetes Type II CC-2928
D-HMVEC-C – 患病的人类心脏微血管内皮细胞 – II 型糖尿病 CC-2928
D-HMVEC-dAD – Diseased Human Dermal Microvascular Endothelial Cells, Adult – Diabetes Type I CC-2929
D-HMVEC-dAD – 患病的人类皮肤微血管内皮细胞，成人 – I 型糖尿病 CC-2929
D-HMVEC-dAD – Diseased Human Dermal Microvascular Endothelial Cells, Adult – Diabetes Type II CC-2930
D-HMVEC-dAD – 患病的人类皮肤微血管内皮细胞，成人 – II 型糖尿病 CC-2930
HMVEC-Bd – Human Bladder Microvascular Endothelial Cells CC-7016
HMVEC-Bd – 人膀胱微血管内皮细胞 CC-7016
lonza,lonza CC-5034,BE02-034E,CC-5002,ReagentPack™ 继代培养试剂
Catalog #: CC-5034
ReagentPackTM Subculture Reagents for primary cell subculturing
用于原代细胞继代培养的 ReagentPackTM Subculture Reagents
Product Overview
ReagentPackTM Subculture Kits have been designed specifically for the passaging of primary cell types. Each kit contains 100 mL each of: Trypsin/EDTA, Trypsin Neutralizing Solution, HEPES Buffered Saline
The ReagentPackTM Subculture Reagents kit is recommended for use with Lonza’s primary cells to ensure effective trypsinization and a successful cell passaging process.
ReagentPackTM Subculture Kits 专为原代细胞类型的传代而设计。 每个试剂盒均包含 100 mL：胰蛋白酶/EDTA、胰蛋白酶中和溶液、HEPES 缓冲盐水
建议将 ReagentPackTM Subculture Reagents 试剂盒用于 Lonza 的原代细胞，以确保有效的胰蛋白酶消化和成功的细胞传代过程。
Content & Storage
Content
1 x Bottle Trypsin/EDTA 100 mL
1 x Bottle Trypsin Neutralizing Solution 100 mL
1 x Bottle HEPES Buffered Saline Solution 100 mL
Instructions, SDS, CoA
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TrypZeanTM recombinant Trypsin EDTA (1x) BE02-034E
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Trypsin Neutralizing Solution, 100 mL CC-5002
胰蛋白酶中和溶液，100 mL CC-5002
Trypsin/EDTA Solution, 100 mL CC-5012
胰蛋白酶/EDTA 溶液，100 mL CC-5012
HEPES Buffered Saline Solution – 100 ml CC-5022
HEPES 缓冲盐溶液 – 100 ml CC-5022
HEPES Buffered Saline Solution, 500 mL CC-5024
HEPES 缓冲盐溶液，500 mL CC-5024
lonza,lonza CC-3170,CC-3171,CC-4175,BEGM™ 支气管上皮细胞生长培养基 BulletKit™
货号: CC-3170
Culture system containing BEBMTM Bronchial Epithelial Cell Growth Basal Medium (CC-3171) and BEGMTM Bronchial Epithelial Cell Growth Medium SingleQuotsTM Supplements and Growth Factors (CC-4175).
Request Information or Quote or do you need a different format or volume for this media product? Contact Lonza CellBio Services to discuss custom format and formulation options.
包含 BEBMTM 支气管上皮细胞生长基础培养基 (CC-3171) 和 BEGMTM 支气管上皮细胞生长培养基 SingleQuotsTM 添加剂和生长因子 (CC-4175) 的培养系统。
请求信息或报价，或者您是否需要此媒体产品的不同格式或音量？ 联系 Lonza CellBio Services 讨论定制形式和配方选项。
Product Overview
BEGMTM Bronchial Epithelial Cell Growth Medium is specially designed to support the growth of human primary bronchial epithelial cells. BEGMTM BulletKitTM is serum-free and has been optimized to grow cells from normal and diseased tissues.
Published research shows BEGMTM BulletKitTM has expanded applications. Our customers have used BEGMTM BulletKitTM to culture well-established cell lines such as BEAS-2B, home-grown bronchial epithelial cells and supporting co-culture models with breast cancer cell lines such as MCF-7 and MDA-MB-231. Refer to the published literature in the ‘Data’ tab.
BEGMTM is an optimized and readily available media kit providing researchers the confidence and the convenience of a system that works to support their cells. This is especially important for researchers who are utilizing cell lines as a stepping stone to primary cell culture. Working with the same BEGMTM Media between cell lines and primary NHBE can reduce variability within the experiments and simplify the shift for researchers. In addition, if primary cells are utilized as a side-by-side control with normal cell lines or home-grown bronchial epithelial cells, the BEGMTMBulletKitTM Media may also offer cost-benefit since customers can use the same media to support multiple cell types.
BEGMTM 支气管上皮细胞生长培养基专为支持人原代支气管上皮细胞的生长而设计。 BEGMTM BulletKitTM 不含血清，经过优化，可从正常和患病组织中培养细胞。
已发表的研究表明 BEGMTM BulletKitTM 已经扩展了应用程序。我们的客户已使用 BEGMTM BulletKitTM 培养成熟的细胞系，如 BEAS-2B、本土支气管上皮细胞，并支持与乳腺癌细胞系如 MCF-7 和 MDA-MB-231 的共培养模型。请参阅“数据”选项卡中已发表的文献。
BEGMTM 是一种经过优化且随时可用的培养基套件，可为研究人员提供支持其细胞的系统的信心和便利性。这对于利用细胞系作为原代细胞培养垫脚石的研究人员来说尤其重要。在细胞系和原代 NHBE 之间使用相同的 BEGMTM 培养基可以减少实验中的变异性并简化研究人员的转变。此外，如果将原代细胞用作与正常细胞系或本土支气管上皮细胞并排的对照，BEGMTMBulletKitTM 培养基还可以提供成本效益，因为客户可以使用相同的培养基来支持多种细胞类型。
Here are publications out of many where our customers have used BEGMTM Growth Media with normal bronchial epithelial cells:
- El Najjar, et al. (2015). PLoSONE 10(2) demonstrates culturing of BEAS-2B bronchial epithelial cell line in BEGMTM Media to examine the effects of cathepsin and furin proteolytic enzymes on viral fusion protein activation in cells
- Hagiwara, et al. (2015). Nature demonstrates BEGMTM Media can ideally support co-culture of Lonza’s HUVEC and NHBE in a 3D environment.
- Rider, et al. (2015). PLoS ONE 10(1) used BEGMTM Media to culture primary human bronchial epithelial cells obtained from non-transplanted normal lungs
- El Najjar 等人。 （2015 年）。 PLoSONE 10(2) 展示了在 BEGMTM 培养基中培养 BEAS-2B 支气管上皮细胞系以检查组织蛋白酶和弗林蛋白酶对细胞中病毒融合蛋白活化的影响
萩原等人。 （2015 年）。 Nature 证明 BEGMTM Media 可以理想地支持 Lonza 的 HUVEC 和 NHBE 在 3D 环境中的共培养。
骑士，等人。 （2015 年）。 PLoS ONE 10(1) 使用 BEGMTM 培养基培养从非移植的正常肺获得的原代人支气管上皮细胞
Purchase our Bronchial Epithelial Cell Culture Kit (catalog#. CC-2540B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of bronchial epithelial cells (catalog #CC-2540) and BEGMTM BulletKitTM Growth Media (catalog#. CC-3170).
购买我们的支气管上皮细胞培养试剂盒（catalog#. CC-2540B）并获得细胞和培养基的折扣价。 该试剂盒由冷冻保存的支气管上皮细胞安瓿（产品目录号#CC-2540）和 BEGMTM BulletKitTM 生长培养基（目录号#CC-3170）组成。
Content & Storage
Content
1 x BEBMTM Basal Medium (CC-3171), 500 mL
1 x BEGMTM SingleQuotsTM Supplement Pack (CC-4175) containing:
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Amber Vial with Retinoic Acid, 0.50 mL
1 x Natural Cap Vial with Transferrin, 0.50 mL
1 x Amber Vial with Triiodothyronine, 0.50 mL
1 x Amber Vial with Epinephrine, 0.50 mL
1 x Green Cap Vial with hEGF, 0.50 mL
Related Products
BEBM™ Bronchial Epithelial Cell Growth Basal Medium CC-3171
BEBM™ 支气管上皮细胞生长基础培养基 CC-3171
BEGMTM Bronchial Epithelial SingleQuotsTM Kit CC-4175
BEGMTM支气管上皮SingleQuotsTM试剂盒CC-4175
Bronchial Epithelial Cells in RAFT™ 3D Cell Culture System
Bronchial Epithelial Cells Grown in BEGM™ BulletKit™ and Cultured in RAFT™ 3D Cell Culture System
Co-culture Experiments with Lonza’s Bronchial Epithelial Cells and BEGM™ BulletKit™ Media
Lonza支气管上皮细胞和 BEGM™ BulletKit™ 培养基的共培养实验
Furukawa, et al. demonstrates BEGM™ BulletKit™ is suitable for culturing Lonza’s NHBEs as well as to support co-cultures with lung epithelial cell line, BEAS-2B, and breast cancer cell lines, MCF-7 and MDA-MB-231. The breast cancer cell line showed differences in morphology and proliferation rates when cultured with primary NHBE cells vs. BEAS-2B cell line. The paper highlights the importance of using primary NHBE cells as a side-by-side control when conducting experiments with normal lung epithelial cell line such as BEAS-2B because the non-transformed primary cells can validate or offset the data generated using cell lines.
古川等人。 演示 BEGM™ BulletKit™ 适用于培养 Lonza 的 NHBE，并支持与肺上皮细胞系 BEAS-2B 和乳腺癌细胞系 MCF-7 和 MDA-MB-231 的共培养。 当与原代 NHBE 细胞与 BEAS-2B 细胞系一起培养时，乳腺癌细胞系在形态和增殖率方面表现出差异。 本文强调了在对正常肺上皮细胞系（如 BEAS-2B）进行实验时使用原代 NHBE 细胞作为并排对照的重要性，因为未转化的原代细胞可以验证或抵消使用细胞系生成的数据。
lonza,CC-3150,CC-3151,CC-4136,CC-3153,MEGM™ 乳腺上皮细胞生长培养基 BulletKit™
货号: CC-3150
Culture system containing MEBMTM Basal Medium (CC-3151) and MEGMTM SingleQuotsTM Supplements (CC-4136) required for growth of Mammary Epithelial Cells.
包含乳腺上皮细胞生长所需的 MEBMTM 基础培养基 (CC-3151) 和 MEGMTM SingleQuotsTM 补充剂 (CC-4136) 的培养系统。
Product Overview
MEGMTMBulletkitTM, is also sold as separate basal medium and supplements.
MEGMTMTM Mammary Epithelial Cell Growth Medium has been optimized for the growth of Mammary Epithelial cells in a serum-free environment. This media system has been referenced in over 130 unique publications primarily focusing on breast cancer research.
MEGMTMBulletkitTM 也作为单独的基础培养基和补充剂出售。
MEGMTMTM 乳腺上皮细胞生长培养基针对乳腺上皮细胞在无血清环境中的生长进行了优化。 该媒体系统已被 130 多种主要关注乳腺癌研究的独特出版物引用。
Did you know our customers have used MEGMTMTM BulletKitTM not only for Lonza’s primary HMECs but also to culture well-established normal breast cell lines such as MCF-10A and MCF-12A?
MEGMTMTM BulletKitTM is optimized and readily available media kit giving researchers the confidence and the convenience of a system that works to support their cells. This is especially important for researchers who are utilizing normal breast cell lines as a stepping stone to primary HMEC cell culture. Working with the same media between cell lines and primary cells can reduce variability within the experiments and simplifies the shift for researchers. In addition, if primary HMECs are utilized as a side-by-side control with normal breast cell lines, the MEGMTMTM BulletKitTM media may also offer cost-benefits since customers can use the same media to support both cell types.
Purchase our Mammary Epithelial Cell Culture Kit (catalog no. CC-2551B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of mammary epithelial cells (catalog no. CC-2551) and MEGMTMTM BulletKitTM Growth Media (catalog no. CC-3150)
MEGMTMTM BulletKitTM 是经过优化且随时可用的培养基套件，为研究人员提供了支持其细胞的系统的信心和便利性。 这对于使用正常乳腺细胞系作为初级 HMEC 细胞培养的垫脚石的研究人员来说尤其重要。 在细胞系和原代细胞之间使用相同的培养基可以减少实验中的变异性并简化研究人员的转变。 此外，如果将原代 HMEC 用作与正常乳腺细胞系的并排对照，MEGMTMTM BulletKitTM 培养基还可以提供成本效益，因为客户可以使用相同的培养基来支持两种细胞类型。
购买我们的乳腺上皮细胞培养试剂盒（目录号 CC-2551B）并获得细胞和培养基的折扣价。 该试剂盒包括一个冷冻保存的乳腺上皮细胞安瓿（货号 CC-2551）和 MEGMTMTM BulletKitTM 生长培养基（货号 CC-3150）
Content & Storage
Content
1 x MEBMTM Basal Medium (CC-3151), 500 mL
1 x MEGMTM SingleQuotsTM Supplement Pack (CC-4136) containing:
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
Safety Data Sheets (SDS)
View SDS
SDS – German
SDS – Italian
SDS – Dutch
SDS – French
SDS – Spanish
SDS – English
Related Products
MEBM™ Mammary Epithelial Cell Growth Basal Medium CC-3151
MEBM™ 乳腺上皮细胞生长基础培养基 CC-3151
MEGMTM Mammary Epithelial Cell Growth Medium SingleQuotsTM Kit CC-4136
MEGMTM 乳腺上皮细胞生长培养基 SingleQuotsTM 试剂盒 CC-4136
MEBM™ Mammary Epithelial Cell Growth Basal Medium, Phenol Red Free CC-3153
MEBM™ 乳腺上皮细胞生长基础培养基，无酚红 CC-3153
Lonza’s HMEC cells cultured in MEGM™ BulletKit™ Medium vs. other commercially available systems
Graph showing the propagation potential of Lonza’s HMEC cells cultured in MEGM™ BulletKit™ Medium vs. other commercially available systems
A Three-Dimensional RAFT™ Co-Culture as Advanced Model for Breast Cancer Drug Discovery
Presented at AACR 2017, we demonstrate here the feasibility of co-culturing human mammary fibroblasts and human mammary epithelial cells in RAFT™ 3D Culture System as a 3D breast cancer model.
Selected publications out of many where our customers have used MEGM™ Growth Media with various normal breast cells.
- Savanur, et al. shows Lonza’s HMECs were cultured in Lonza’s MEGM™ Media alongside breast cancer cell lines in the respective media to understand the inhibitory effects of Lectin on proliferation of breast cancer cells
- Park, et. al. PLoS ONE 10(6) demonstrates how MCF-10A and MCF-12A were cultured in MEGM™™ alongside breast cancer cell lines in their respective media to examine the effects of cold atmospheric plasma on epigenetic alterations
- Kmiecik, et al. 2015. PLoS ONE 10(5) shows MEGM™ Medium’s compatibility with immortalized hTERT-HME1 cells in a study of MT3-overexpression on proliferation, invasiveness, migration,and tumorigenesis of breast cancer cells
- 萨瓦努尔等人。 显示 Lonza 的 HMEC 在 Lonza 的 MEGM™ 培养基中与相应培养基中的乳腺癌细胞系一起培养，以了解凝集素对乳腺癌细胞增殖的抑制作用
公园等 人。 PLoS ONE 10(6) 展示了 MCF-10A 和 MCF-12A 如何在 MEGM™ 中与乳腺癌细胞系一起在各自的培养基中培养，以检查冷大气血浆对表观遗传改变的影响
Kmiecik 等人。 2015. PLoS ONE 10(5) 在 MT3 过表达对乳腺癌细胞增殖、侵袭性、迁移和肿瘤发生的研究中显示了 MEGM™ 培养基与永生化 hTERT-HME1 细胞的相容性
lonza,CC-3156,CC-2920,CC-3162,CC-3202,EBM™-2 内皮细胞生长基础培养基-2
货号: CC-3156
EBMTM-2 Endothelial Cell Growth Basal Medium recommended for growth of Endothelial cells (basal media only). Required growth factors and supplements sold seperately.
Available for immediate shipment.
Request Information or Quote or do you need a different format or volume for this media product? Contact Lonza CellBio Services to discuss custom format and formulation options.
EBMTM-2 内皮细胞生长基础培养基推荐用于内皮细胞的生长（仅基础培养基）。 所需的生长因子和补充剂单独出售。
可立即发货。
请求信息或报价，或者您是否需要此媒体产品的不同格式或音量？ 联系 Lonza CellBio Services 讨论定制形式和配方选项。
Product Overview
EBMTM -2 is an endothelial basal medium sold separately. This can be used with 1 x kit of CC-4176 (Large-vessel endothelial cell growth medium SingleQuotsTM Supplements and Growth Factors) or 1 x CC-4147 (Microvascular endothelial cell growth medium SingleQuotsTM Supplements and Growth Factors).
Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used to grow CloneticsTM Primary Cells.
- EGMTM-2 BulletKitTM (CC-3162) – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Contains 2% FBS and VEGF for rapid proliferation. Does not contain BBE.
- EGMTM-2 MV BulletKitTM (CC-3202) – Optimized for Coronary and Iliac artery endothelial cells and human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. Does not contain BBE.
Lonza guarantees the performance of CloneticsTM/PoieticsTM Cells only if appropriate CloneticsTM/PoieticsTM Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.
EBMTM -2 是一种内皮基础培养基，单独出售。这可以与 1 x 试剂盒 CC-4176（大血管内皮细胞生长培养基 SingleQuotsTM 补充剂和生长因子）或 1 x CC-4147（微血管内皮细胞生长培养基 SingleQuotsTM 补充剂和生长因子）一起使用。
从多种内皮细胞生长培养基中进行选择，以轻松培养原代内皮细胞。每种内皮细胞生长培养基都有其特殊的配方，并针对某些细胞类型进行了优化。每种类型的内皮细胞生长培养基在用于培养 CloneticsTM 原代细胞时都能保证发挥作用。
EGMTM-2 BulletKitTM (CC-3162) – 针对某些 HUVEC、主动脉和肺动脉内皮细胞进行了优化。含有 2% FBS 和 VEGF 用于快速增殖。不含 BBE。
EGMTM-2 MV BulletKitTM (CC-3202) – 针对冠状动脉和髂动脉内皮细胞以及人类微血管内皮细胞进行了优化，含有 5% FBS 和 VEGF，可快速增殖。不含 BBE。
只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
Content & Storage
Content
1 x Bottle EBMTM-2 Basal Medium, 500 mL
Instructions, SDS, CoA
Related Products
EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™ CC-3162
EGMTM -2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM CC-3202
HMVEC-dNeo – Human Dermal Microvascular Endothelial Cells, Neonatal, Single Donor CC-2505
HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2 C2517A
HUVEC-XL – Human Umbilical Vein Endothelial Cells, Large Volume, Pooled 00191027
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™-2 C2519A
HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2, Prescreened for Angiogenesis C2517AS
HMVEC-L – Human Lung Microvascular Endothelial Cells CC-2527
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™-2, Prescreened for Angiogenesis C2519AS
HPAEC – Human Pulmonary Artery Endothelial Cells CC-2530
HPAEC – 人肺动脉内皮细胞 CC-2530
HAEC – Human Aortic Endothelial Cells CC-2535
HAEC – 人主动脉内皮细胞 CC-2535
HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult CC-2543
HMVEC-dAd – 人真皮微血管内皮细胞 – 成人 CC-2543
HCAEC – Human Coronary Artery Endothelial Cells CC-2585
HCAEC – 人冠状动脉内皮细胞 CC-2585
HIAEC – Human Iliac Artery Endothelial Cells CC-2545
HIAEC – 人髂动脉内皮细胞 CC-2545
D-HAEC – Diseased Human Aortic Endothelial Cells – Diabetes Type I CC-2919
D-HAEC – 患病的人主动脉内皮细胞 – I 型糖尿病 CC-2919
D-HAEC – Diseased Human Aortic Endothelial Cells – Diabetes Type II CC-2920
D-HAEC – 患病的人主动脉内皮细胞 – II 型糖尿病 CC-2920
lonza,lonza PT-2501,LT07-218,PT-5006,PT-3003,hMSC – 人间充质干细胞
Catalog #: PT-2501
Cryopreserved ampule of Human Mesenchymal Stem Cells (hMSCs) containing ≥750,000 cells
含有≥750,000 个细胞的人间充质干细胞 (hMSCs) 冻存安瓿
Product Overview
PoieticsTM Normal Human Bone Marrow Derived Mesenchymal Stem Cells (hMSC) are isolated from normal (non-diabetic) adult human bone marrow withdrawn from bilateral punctures of the posterior iliac crests of normal volunteers. Normal Human Bone Marrow Derived Mesenchymal Stem Cells (hMSC) have been reported to differentiate down many different lineages including chondrogenic, osteogenic, adipogenic and neural. Normal Human Bone Marrow Derived Mesenchymal Stem Cells (hMSC) have been cryopreserved after the second passage and are suggested to be used by no later than the fifth passage. Cryopreserved PoieticsTM Normal Human Bone Marrow Derived Mesenchymal Stem Cells (hMSC) are guaranteed to have at least 0.75 million viable cells per vial, differentiate down the adipogenic, chondrogenic, and osteogenic liniages when cultured in the recommended differentiation medium, to express CD29, CD44, CD105, and CD166 and to not express CD14, CD34, and CD45. All lots of this product manufactured after January 1, 2015 are also guaranteed to express CD90 and CD73 and to not express HLA-DR or CD19.
All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.
PoieticsTM 正常人骨髓来源的间充质干细胞 (hMSC) 是从正常志愿者的髂后嵴双侧穿刺提取的正常（非糖尿病）成人骨髓中分离出来的。据报道，正常人骨髓来源的间充质干细胞 (hMSC) 可分化出许多不同的谱系，包括软骨形成、成骨、脂肪形成和神经。正常人骨髓来源的间充质干细胞（hMSC）在第二代后被冷冻保存，建议不迟于第五代使用。冷冻保存的 PoieticsTM 正常人骨髓来源的间充质干细胞 (hMSC) 保证每瓶至少有 75 万个活细胞，在推荐的分化培养基中培养时，可分化成脂肪、软骨和成骨细胞，以表达 CD29、CD44、 CD105 和 CD166 并且不表达 CD14、CD34 和 CD45。 2015 年 1 月 1 日之后生产的所有批次产品也保证表达 CD90 和 CD73，不表达 HLA-DR 或 CD19。
所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
Benefits
- Ready to use
- Each lot tested and verified to differentiate down each of the three lineages (adipogenic, chondrogenic, osteogenic)
- Multiple donor availability
Applications
- Regenerative medicine
- Gene therapy
- Cell differentiation and gene regulation
- Cell-based screening assays
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MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ PT-3001
MSCGM™ 间充质干细胞生长培养基 BulletKit™ PT-3001
Human Mesenchymal Stem Cell (hMSC) Osteogenic Differentiation Medium BulletKitTM PT-3002
人间充质干细胞 (hMSC) 成骨分化培养基 BulletKitTM PT-3002
Human Mesenchymal Stem Cell (hMSC) Adipogenic Differentiation Medium BulletKitTM PT-3004
人间充质干细胞 (hMSC) 脂肪分化培养基 BulletKitTM PT-3004
Human Mesenchymal Stem Cell (hMSC) Chondrogenic Differentiation Medium BulletKitTM PT-3003
人间充质干细胞 (hMSC) 软骨分化培养基 BulletKitTM PT-3003
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1012
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P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1024
P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1024
P1 Primary Cell 4D-Nucleofector™ X Kit S V4XP-1032
P1 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-1032
P1 Primary Cell 96-well Nucleofector™ Kit V4SP-1096
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P1 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-1960
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hADSC 人体脂肪干细胞 PT-5006
DPSC – Human Dental Pulp Stem Cells PT-5025
DPSC – 人牙髓干细胞 PT-5025
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用于 hMSC 软骨分化试剂盒 PT-4124 的 TGF Beta 3
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
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lonza,lonza CC-4176,CC-3162,C2517A,C2517AS,EGM-2 内皮 SingleQuots 试剂盒
货号: CC-4176
Contains supplements and growth factors required for culturing Endothelial cells (basal media not included)
包含培养内皮细胞所需的补充剂和生长因子（不包括基础培养基）
Product Overview
EGMTM -2 SingleQuotTM Kit are components sold separately and does not include the basal medium. These are part of the EGMTM -2 Endothelial Cell Growth Medium BulletKitTM (Catalog Number CC-3162) which consists of basal medium and supplements packaged together. Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used to grow CloneticsTM Primary Cells.
EGMTM -2 SingleQuotTM 套件是单独出售的组件，不包括基础培养基。 这些是 EGMTM -2 内皮细胞生长培养基 BulletKitTM（目录号 CC-3162）的一部分，它由包装在一起的基础培养基和补充剂组成。 从多种内皮细胞生长培养基中进行选择，以轻松培养原代内皮细胞。 每种内皮细胞生长培养基都有其特殊的配方，并针对某些细胞类型进行了优化。 每种类型的内皮细胞生长培养基在用于培养 CloneticsTM 原代细胞时都能保证发挥作用。
The following Endothelial Cell Growth Medium BulletKitsTM are available:
- EGMTM-2 BulletKitTM (CC-3162) – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Contains 2% FBS and VEGF for rapid proliferation. Does not contain BBE.
- EGMTM BulletKitTM (CC-3124) – Optimized for HUVECs cat. no. CC-2517 and CC-2519, contains 2% FBS and BBE. Does not contain exogenous VEGF.
- EGMTM-Plus BulletKitTM (CC-5035) – Enhanced version of EGMTM Medium. Optimized for HUVECs cat. no. CC-2935. Contains 2% FBS and BBE. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGMTM Medium.
- EGMTM-2 MV BulletKitTM (CC-3202) – Coronary and Iliac artery, human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. Does not contain BBE.
Lonza guarantees the performance of CloneticsTM/PoieticsTM Cells only if appropriate CloneticsTM/PoieticsTM Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.
只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
Content & Storage
Content
1 x Bottle FBS 10.00 mL
1 x Natural Cap Vial with Hydrocortisone 0.20 mL
1 x Gray Cap Vial with hFGF-B 2.00 mL
1 x White Cap Vial with VEGF 0.50 mL
1 x Yellow Cap Vial with R3-IGF-1 0.50 mL
1 x Blue Cap Vial with Ascorbic Acid 0.50 mL
1 x Green Cap Vial with hEGF 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Orange Cap Vial with Heparin 0.50 mL
Related Products
EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™ CC-3162
EGM™-2 内皮细胞生长培养基-2 BulletKit™ CC-3162
HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2 C2517A
HUVEC – EGM™-2 C2517A 中的人脐静脉内皮细胞，单一供体
HUVEC-XL – Human Umbilical Vein Endothelial Cells, Large Volume, Pooled 00191027
HUVEC-XL – 人脐静脉内皮细胞，大容量，混合 00191027
HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2, Prescreened for Angiogenesis C2517AS
HUVEC – 人脐静脉内皮细胞，单一供体，在 EGM™-2 中，预先筛选血管生成 C2517AS
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™-2, Prescreened for Angiogenesis C2519AS
HUVEC – 人脐静脉内皮细胞，汇集在 EGM™-2 中，预先筛选血管生成 C2519AS
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™-2 C2519A
HUVEC – 人脐静脉内皮细胞，混合，在 EGM™-2 C2519A 中
HPAEC – Human Pulmonary Artery Endothelial Cells CC-2530
HPAEC – 人肺动脉内皮细胞 CC-2530
HAEC – Human Aortic Endothelial Cells CC-2535
HAEC – 人主动脉内皮细胞 CC-2535
D-HAEC – Diseased Human Aortic Endothelial Cells – Diabetes Type II CC-2920
D-HAEC – 患病的人主动脉内皮细胞 – II 型糖尿病 CC-2920
D-HAEC – Diseased Human Aortic Endothelial Cells – Diabetes Type I CC-2919
D-HAEC – 患病的人主动脉内皮细胞 – I 型糖尿病 CC-2919
D-HPAEC – Diseased Human Pulmonary Artery Endothelial Cells – Diabetes Type I CC-2923
D-HPAEC – 患病的人肺动脉内皮细胞 – I 型糖尿病 CC-2923
D-HPAEC – Diseased Human Pulmonary Artery Endothelial Cells – Diabetes Type II CC-2924
D-HPAEC – 患病的人肺动脉内皮细胞 – II 型糖尿病 CC-2924
lonza,lonza CC-3124,lonza CC-4133,CC-3121,EGM™ 内皮细胞生长培养基 BulletKit™
货号: CC-3124
Culture system containing EBMTM Basal Medium (CC-3121) and EGMTM Endothelial Cell Growth Medium SingleQuotsTM Supplements (CC-4133) required for growth of endothelial cells
包含内皮细胞生长所需的 EBMTM 基础培养基 (CC-3121) 和 EGMTM 内皮细胞生长培养基 SingleQuotsTM 补充剂 (CC-4133) 的培养系统
Product Overview
Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used to grow CloneticsTM Primary Cells.
从多种内皮细胞生长培养基中进行选择，以轻松培养原代内皮细胞。 每种内皮细胞生长培养基都有其特殊的配方，并针对某些细胞类型进行了优化。 每种类型的内皮细胞生长培养基在用于培养 CloneticsTM 原代细胞时都能保证发挥作用。
The following Endothelial Cell Growth Medium BulletKitsTM are available:
- EGMTM-2 BulletKitTM (CC-3162) – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Contains 2% FBS and VEGF for rapid proliferation. Does not contain BBE.
- EGMTM BulletKitTM (CC-3124) – Optimized for HUVECs cat. no. CC-2517 and CC-2519, contains 2% FBS and BBE. Does not contain exogenous VEGF.
- EGMTM-Plus BulletKitTM (CC-5035) – Enhanced version of EGMTM Medium. Optimized for HUVECs cat. no. CC-2935. Contains 2% FBS and BBE. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGMTM Medium.
- EGMTM-2MV BulletKitTM (CC-3202) – Coronary and Iliac artery, human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. Does not contain BBE.
Lonza guarantees the performance of CloneticsTM/PoieticsTM Cells only if appropriate CloneticsTM/PoieticsTM Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.
只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
Content & Storage
Content
1 x EBMTM Basal Medium (CC-3121), 500 mL
1 x EGMTM SingleQuotsTM Supplement Pack (CC-4133) containing:
1 x Green Cap Vial with hEGF 0.50 mL
1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 Bottle BBE 2.00 mL
1 x Blue Cap Vial with Ascorbic Acid 0.50 mL
1 Bottle FBS 10.00 mL
Related Products
EBM™ Endothelial Cell Growth Basal Medium, 500 mL CC-3121
EBM™ 内皮细胞生长基础培养基，500 mL CC-3121
EGMTM Endothelial Cell Growth Medium SingleQuotsTM Kit CC-4133
EGMTM 内皮细胞生长培养基 SingleQuotsTM 试剂盒 CC-4133
lonza,lonza C2517A,016-1R16,HUVEC – EGM™-2 中的人脐静脉内皮细胞
货号: C2517A
Cryopreserved ampule of Human Umbilical Vein Endothelial Cells (HUVEC), from a single donor, in EGMTM-2 containing ≥ 500,000 cells
来自单一供体的人脐静脉内皮细胞 (HUVEC) 安瓿冻存于 EGMTM-2 中，含有 ≥ 500,000 个细胞
Product Overview
Lonza’s Cryopreserved HUVECs are shipped as frozen primaries. They are guaranteed to express CD31/105 and survive in culture through 15* population doublings (*except HUVEC-XLTM guaranteed through 5 PD).
All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Our pooled vs. single donor options allow researchers to study or exclude donor variability from their experiments. Depending upon the study, pooled version is typically utilized to reduce donor variability while single donor option allows researchers to assess donor-specific differences.
HUVEC-XL size consists of 10 million cells/amp achieved by pooling donors. This option is designed to support studies that require large volume of cells while still offering cost-benefits to researchers.
Lonza 的冷冻保存的 HUVEC 作为冷冻初级产品运输。 它们保证表达 CD31/105 并通过 15* 种群倍增在培养中存活（* HUVEC-XLTM 除外，保证通过 5 PD）。
所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。
我们的汇集与单一供体选项允许研究人员在他们的实验中研究或排除供体变异性。 根据研究，汇总版本通常用于减少供体变异性，而单一供体选项允许研究人员评估特定供体的差异。
HUVEC-XL 大小由 1000 万个细胞/安培组成，通过汇集供体实现。 此选项旨在支持需要大量细胞的研究，同时仍为研究人员提供成本效益。
Not All HUVECs Are Created Equal – Which Type Is Suitable for Your Research?
- C2517A (single donor), C2519A (pooled donor), 00191027 (XL size) – Isolated in EGMTM-2 Media, in presence of growth factors such as VEGF, rhFGF, rhEGF, and others. Does not contain BBE.
- C2517AS (single donor), C2519AS (pooled donor) – Pre-screened HUVECs in EGMTM-2 Media. Screened for angiogenesis markers and test positive for expression of eNOS, Axl, Tie-2 and VEGFr2.
- CC-2517 (single donor), CC-2519 (pooled donor) – Both isolated in EGMTM Media, in absence of defined growth factors. Contains BBE. No exogenous VEGF.
- CC-2935 (single donor) – Isolated in EGMTM – Plus Media: Improved version of part number CC-2517. Offers faster proliferation rates than HUVEC isolated in EGMTM Media. No exogenous VEGF.
Benefits
- Guaranteed through 15 population doublings
- Test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- A Certificate of Analysis is provided for each cell lot purchased
Applications
- Would healing
- Angiogenesis
- Tissue engineering
- Inflammation
- Oncology
- Pharmacology
- Vascular modeling
- Transfection
Content & Storage
Content
1 x Cryopreserved ampule of HUVEC containing ≥ 500,000 cells
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HCAEC – Human Coronary Artery Endothelial Cells CC-2585
HCAEC – 人冠状动脉内皮细胞 CC-2585
HAEC – Human Aortic Endothelial Cells CC-2535
HAEC – 人主动脉内皮细胞 CC-2535
HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2, Prescreened for Angiogenesis C2517AS
HUVEC – 人脐静脉内皮细胞，单一供体，在 EGM™-2 中，预先筛选血管生成 C2517AS
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™-2 C2519A
HUVEC – 人脐静脉内皮细胞，混合，在 EGM™-2 C2519A 中
HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™ CC-2517
HUVEC – EGM™ CC-2517 中的人脐静脉内皮细胞，单一供体
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™ CC-2519
HUVEC – 人脐静脉内皮细胞，汇集，在 EGM™ CC-2519 中
HUVEC – Human Umbilical Vein Endothelial Cells without VEGF, Single Donor, in EGM™-Plus CC-2935
HUVEC – EGM™-Plus CC-2935 中不含 VEGF 的人脐静脉内皮细胞，单一供体
lonza,lonza 00190860,EBMTM-2 内皮细胞生长基础培养基-2
Catalog #: 00190860
Basal media required for growth of endothelial cells, 1 L (supplements and growth factors sold separately)
内皮细胞生长所需的基础培养基，1 L（补充剂和生长因子单独出售）
Product Overview
EBMTM -2 is an endothelial basal medium sold separately. This can be used with 2 x kits of CC-4176 (Large-vessel endothelial cell growth medium SingleQuotsTM Supplements and Growth Factors) or 2 x CC-4147 (Microvascular endothelial cell growth medium SingleQuotsTM Supplements and Growth Factors).
Lonza guarantees the performance of CloneticsTM/PoieticsTM Cells only if appropriate CloneticsTM/PoieticsTM Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.
EBMTM -2 是一种内皮基础培养基，单独出售。 这可以与 2 x CC-4176（大血管内皮细胞生长培养基 SingleQuotsTM 补充剂和生长因子）或 2 x CC-4147（微血管内皮细胞生长培养基 SingleQuotsTM 补充剂和生长因子）试剂盒一起使用。
只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
Content & Storage
Content
1 x Bottle Basal Medium 1 Liter
HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2 C2517A
HMVEC-dNeo – Human Dermal Microvascular Endothelial Cells, Neonatal, Single Donor CC-2505
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™-2 C2519A
HUVEC-XL – Human Umbilical Vein Endothelial Cells, Large Volume, Pooled 00191027
HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2, Prescreened for Angiogenesis C2517AS
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™-2, Prescreened for Angiogenesis C2519AS
HMVEC-L – Human Lung Microvascular Endothelial Cells CC-2527
HPAEC – Human Pulmonary Artery Endothelial Cells CC-2530
HAEC – Human Aortic Endothelial Cells CC-2535
HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult CC-2543
HIAEC – Human Iliac Artery Endothelial Cells CC-2545
D-HAEC – Diseased Human Aortic Endothelial Cells – Diabetes Type I CC-2919
D-HCAEC – Diseased Human Coronary Artery Endothelial Cells – Diabetes Type I CC-2921
D-HCAEC – 患病的人冠状动脉内皮细胞 – I 型糖尿病 CC-2921
D-HAEC – Diseased Human Aortic Endothelial Cells – Diabetes Type II CC-2920
D-HAEC – 患病的人主动脉内皮细胞 – II 型糖尿病 CC-2920
HCAEC – Human Coronary Artery Endothelial Cells CC-2585,
HCAEC – 人冠状动脉内皮细胞 CC-2585
D-HCAEC – Diseased Human Coronary Artery Endothelial Cells – Diabetes Type II CC-2922
D-HCAEC – 患病的人类冠状动脉内皮细胞 – II 型糖尿病 CC-2922
lonza,CC-3132,00195277,CC-3131,FGM™-2 成纤维细胞生长培养基-2 BulletKit™
Catalog #: CC-3132
Culture system contains FBMTM Basal Medium (CC-3131) and FGMTM-2 SingleQuotsTM supplements (CC-4126) required for growth of fibroblasts.
培养系统包含成纤维细胞生长所需的 FBMTM 基础培养基 (CC-3131) 和 FGMTM-2 SingleQuotsTM 补充剂 (CC-4126)。
Product Overview
FGMTM-2 Fibroblast Growth Medium-2 was developed to support the growth of most primary human fibroblasts including lung and dermal fibroblasts. The kit contains basal medium and supplements packaged together for convenience to culture fibroblasts. FGMTM-2 Medium contains 2% serum.
Purchase our Lung Fibroblast Cell Culture Kit (catalog no. CC-2512B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of lung fibroblast cells (catalog no. CC-2512) and FGMTM-2 BulletKitTM Growth Media (catalog no. CC-3132)
FGMTM-2 Fibroblast Growth Medium-2 旨在支持大多数原代人类成纤维细胞的生长，包括肺和真皮成纤维细胞。 该试剂盒包含包装在一起的基础培养基和补充剂，以方便培养成纤维细胞。 FGMTM-2 培养基含有 2% 血清。
购买我们的肺成纤维细胞培养试剂盒（目录号 CC-2512B）并获得细胞和培养基的折扣价。 该试剂盒由冷冻保存的肺成纤维细胞安瓿（货号 CC-2512）和 FGMTM-2 BulletKitTM 生长培养基（货号 CC-3132）组成
Content & Storage
Content
1 x FBMTM Basal Medium (CC-3131), 500 mL
1 x FGMTM-2 SingleQuotsTM Supplement Pack (CC-4126) containing:
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Gray Cap Vial with hFGF-B, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Bottle FBS, 10.00 mL
Instructions, SDS, CoA
Related Products
FBM™ Fibroblast Growth Basal Medium, 500 mL CC-3131
FBM™ 成纤维细胞生长基础培养基，500 mL CC-3131
FGM™-2 Fibroblast Growth Medium-2 SingleQuots™ Supplements and Growth Factors CC-4126
FGM™-2 成纤维细胞生长培养基-2 SingleQuots™ 补充剂和生长因子 CC-4126
NHDF-Ad – Human Dermal Fibroblasts, Adult CC-2511
NHDF-Ad – 人皮肤成纤维细胞，成人 CC-2511
NHDF-Neo – Human Dermal Fibroblasts, Neonatal CC-2509
NHDF-Neo – 人类皮肤成纤维细胞，新生儿 CC-2509
D-HLF-As – Diseased Human Lung Fibroblast Cells – Asthma 00194912
D-HLF-As – 患病的人肺成纤维细胞 – 哮喘 00194912
D-HLF-COPD – Diseased Human Lung Fibroblast Cell – COPD 00195277
D-HLF-COPD – 患病的人肺成纤维细胞 – COPD 00195277
NHLF – Human Lung Fibroblasts CC-2512
NHLF – 人肺成纤维细胞 CC-2512
lonza,CC-2702,LT07-218,CC-3211,人外周血单个核细胞 (hPBMC)
货号: CC-2702
Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 50 million cells
含有 ≥ 5000 万个细胞的人外周血单个核细胞 (hPBMC) 冻存安瓿
Product Overview
Lonza is your ideal partner when you are looking to buy PBMC. Our Peripheral Blood Mononuclear Cells (PBMCs) are isolated from peripheral blood by apheresis and density gradient separation. They are guaranteed to contain ≥50 million viable cells per ampoule. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Ability to Screen Different Donors
Sample representation in your experiment matters. We maintain an inventory of different donors in an effort to satisfy your sample representation requirements. You can choose a lot based on donor demographic characteristics, and screen multiple lots to find the lot(s) that work best for you. In addition, we can request repeat donors if you need additional cells from a particular donor.
Rich Source of Immune Cells
Peripheral blood mononuclear cells (PBMC) are a mixed population of single nucleus cells. They can be further purified to isolate individual cell types such as natural killer cells, T cells, and B cells. In addition, PMBCs are a rich source of monocytes, which can be directed to differentiate into either macrophages or dendritic cells through culture with various cytokines. Supported by an Optimized Media System LGM-3TM Lymphocyte Growth Medium was specifically designed for support and maintenance of lymphocytes and is optimized for serum-free culturing (additional cytokines may be necessary depending on application) Alternative media containing serum may also be acceptable.
Please contact Scientific Support for serum containing media recommendations. Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.
当您想购买 PBMC 时，Lonza 是您理想的合作伙伴。我们的外周血单个核细胞 (PBMC) 通过单采和密度梯度分离从外周血中分离出来。它们保证每个安瓿含有≥5000万个活细胞。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。
筛选不同捐赠者的能力
实验中的样本表示很重要。我们保留一份不同捐赠者的清单，以满足您的样本代表要求。您可以根据捐赠者的人口统计特征选择批次，并筛选多个批次以找到最适合您的批次。此外，如果您需要来自特定捐赠者的额外细胞，我们可以请求重复捐赠者。
丰富的免疫细胞来源
外周血单个核细胞 (PBMC) 是单核细胞的混合群体。它们可以进一步纯化以分离单个细胞类型，例如自然杀伤细胞、T 细胞和 B 细胞。此外，PMBC 是单核细胞的丰富来源，可通过与各种细胞因子一起培养，定向分化为巨噬细胞或树突状细胞。由优化的培养基系统支持 LGM-3TM 淋巴细胞生长培养基专为支持和维持淋巴细胞而设计，并针对无血清培养进行了优化（根据应用，可能需要额外的细胞因子） 也可以接受含有血清的替代培养基。
有关含血清培养基的建议，请联系科学支持。只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
Benefits
- Test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis (CoA) is provided for each cell lot purchased
Applications
- ELISPOT
- Proliferation assays
- Immunogenicity assays
- Cell therapy process development
- Humanized mice
- Biomarker discovery
Content & Storage
Content
1 x Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 50 million cells
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NK – Human Natural Killer Cells 2W-502
NK – 人类自然杀伤细胞 2W-502
LGM-3TM Lymphocyte Growth Medium-3 CC-3211
LGM-3TM 淋巴细胞生长培养基-3 CC-3211
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218
lonza,CC-2565,V5SP-3002,016-1R16,AC-2509,NHA-人类星形胶质细胞
货号: CC-2565
Cryopreserved ampule of Normal Human Astrocytes (NHA) containing ≥1 million cells
冷冻保存的正常人星形胶质细胞 (NHA) 安瓿，含有 ≥ 100 万个细胞
Product Overview
Normal Human Astrocytes (NHA) can be used to study the function of the central nervous system and how neural cells interact. Astrocytes are glial cells found in the brain and spinal cord that play a critical role in maintenance, support, and repair of nervous tissue. Cryopreserved NHA are shipped as frozen primary-derived cultures.
Cryopreserved CloneticsTM Normal Human Astrocytes are guaranteed through 10 population doublings, express GFAP. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
正常人类星形胶质细胞 (NHA) 可用于研究中枢神经系统的功能以及神经细胞如何相互作用。 星形胶质细胞是在大脑和脊髓中发现的神经胶质细胞，在维持、支持和修复神经组织方面发挥着关键作用。 冷冻保存的 NHA 作为冷冻原代培养物运送。
冷冻保存的 CloneticsTM 正常人类星形胶质细胞通过 10 次群体倍增得到保证，表达 GFAP。 所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。
Did you know that by using our RAFTTM 3D Cell Culture System normal human astrocytes have been successfully used in blood-brain-barrier models?
Benefits
- Guaranteed through 10 population doublings
- Positive immunofluorescence staining for glial fibrillary acid protein (GFAP)
- Test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- A Certificate ofAnalysis is provided for each cell lot purchased
Applications
- Astrocyte-mediated neurotoxicity
- Neurogenesis research
- Injury
- Drug development
- Parkinson’s disease
- Alzheimer’s disease
Content & Storage
Content
1 x Cryopreserved ampule of NHA containing ≥1 million cells
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Bovine Brain Microvascular Endothelial Cells AC-2509
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P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024
P3 原代细胞 4D-Nucleofector® X 试剂盒 L V4XP-3024
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P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002
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P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010
lonza,PT-3001,PT-2501,CC-3232,MSCGM™ 间充质干细胞生长培养基 BulletKit™
货号: PT-3001
Culture system containing MSCBMTM Basal Media (PT-3238) and MSCGMTM SingleQuots Supplement Kit (PT-4105) required for proliferation of human bone marrow derived mesenchymal stem cells.
包含人骨髓来源的间充质干细胞增殖所需的 MSCBMTM 基础培养基 (PT-3238) 和 MSCGMTM SingleQuots Supplement Kit (PT-4105) 的培养系统。
Product Overview
Stem cells possess the unique ability to proliferate as an undifferentiated cell type, then, when properly stimulated, differentiate into multiple lineages of cells. Lonza’s MSCGMTMhuman Mesenchymal Stem Cell Growth BulletKitTM Medium is a serum containing medium designed to proliferate human bone marrow derived mesenchymal cells in an undifferentiated state.
The serum used in Lonza’s MSCGMTM Human Mesenchymal Stem Cell Growth Medium BulletKitTM Medium is specifically screened to ensure that the serum neither promotes spontaneous differentiation of cells nor inhibits cell differentiation to osteogenic, chondrogenic, or adipogenic lineages (when properly stimulated with an appropriate differentiation media).
The MSCGMTM Human Mesenchymal Stem Cell Growth BulletKitTM Medium is offered as a BulletKitTM Medium (catalog no. PT-3001) which includes both the basal media and the necessary supplements for proliferation of human bone marrow derived mesenchymal stem cells. The basal medium (MSCBMTMhMSC Basal Medium; catalog no. PT-3238) and the necessary supplements (MSCGMTMhMSC SingleQuot Kit; catalog no. PT-4105) can also be purchased individually. Trypsin/EDTA (catalog no. CC-3232): Lonza’s recommended Trypsin/EDTA for all strongly adherent primary cells including normal human articular chondrocytes (NHAC-Kn), human bone marrow derived mesenchymal stem cells (hMSC), and dental pulp stem cells (DPSC).
Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.
干细胞具有作为未分化细胞类型增殖的独特能力，然后在适当刺激时分化成多个细胞谱系。 Lonza 的 MSCGMTMhuman 间充质干细胞生长 BulletKitTM 培养基是一种含有血清的培养基，旨在增殖处于未分化状态的人骨髓来源的间充质细胞。
Lonza 的 MSCGMTM 人间充质干细胞生长培养基 BulletKitTM 培养基中使用的血清经过专门筛选，以确保血清既不促进细胞的自发分化，也不抑制细胞分化为成骨、软骨或脂肪谱系（当用适当的分化培养基适当刺激时） .
MSCGMTM 人间充质干细胞生长 BulletKitTM 培养基以 BulletKitTM 培养基（目录号 PT-3001）的形式提供，其中包括基础培养基和人骨髓衍生间充质干细胞增殖所需的补充剂。基础培养基（MSCBMTMhMSC 基础培养基；目录号 PT-3238）和必要的补充剂（MSCGMTMhMSC SingleQuot 试剂盒；目录号 PT-4105）也可以单独购买。胰蛋白酶/EDTA（目录号 CC-3232）：Lonza 推荐用于所有强粘附原代细胞的胰蛋白酶/EDTA，包括正常人关节软骨细胞 (NHAC-Kn)、人骨髓间充质干细胞 (hMSC) 和牙髓干细胞(DPSC)。
只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
Content & Storage
Content
1 x MSCBMTM Basal Media (PT-3238), 500 mL
1 x MSCGMTM SingleQuotsTM Growth Supplement Kit (PT-4105)
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Human Mesenchymal Stem Cell (hMSC) Chondrogenic Differentiation Medium BulletKitTM PT-3003
人间充质干细胞 (hMSC) 软骨分化培养基 BulletKitTM PT-3003
TGF Beta 3 for hMSC Chondrogenic Differentiation Kit PT-4124
用于 hMSC 软骨分化试剂盒 PT-4124 的 TGF Beta 3
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人间充质干细胞 (hMSC) 脂肪分化培养基 BulletKitTM PT-3004
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218
lonza,00192060,00192151,00192152,KGM™ 黄金角质形成细胞生长培养基 BulletKit™
货号: 00192060
Culture system containing KBMTM GoldTM Basal Medium (00192151) and KGMTM GoldTM SingleQuotsTMsupplements (00192152) required for growth of human keratinocytes.
包含人类角质形成细胞生长所需的 KBMTM GoldTM 基础培养基 (00192151) 和 KGMTM GoldTM SingleQuotsTM 补充剂 (00192152) 的培养系统。
Product Overview
A new robust and versatile serum-free medium formulation for high and low density NHEK-Normal Human Epidermal Keratinocyte cultures
KGM-GoldTM Keratinocyte Growth Medium is designed to support both clonal growth and high density keratinocyte proliferation. The medium supports superior growth and morphology of keratinocytes isolated in KGMTM-2, KGMTM and Competitor A’s Medium (data shown in the data tab)
Furthermore, quality control at Lonza requires that keratinocytes pass strict morphological criteria specifying the minimal presence of enlarged and vacuolated cells, enabling Lonza keratinocytes to be used by the researcher for ≥15 Population Doublings.
Purchase our Adult Keratinocyte Cell Culture Kit (catalog no. 192627B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of adult keratinocytes (catalog no. 192627) and KGMTM-Gold BulletKitTM Growth Media (catalog no. 192060)
用于高密度和低密度 NHEK-Normal 人表皮角质形成细胞培养的新型稳健且多功能的无血清培养基配方
KGM-GoldTM 角质形成细胞生长培养基旨在支持克隆生长和高密度角质形成细胞增殖。 该培养基支持在 KGMTM-2、KGMTM 和竞争对手 A 的培养基中分离的角质形成细胞的出色生长和形态（数据显示在数据选项卡中）
此外，Lonza 的质量控制要求角质形成细胞通过严格的形态学标准，规定最小存在扩大和空泡细胞，使研究人员能够将 Lonza 角质形成细胞用于≥15 人口倍增。
购买我们的成人角质形成细胞培养试剂盒（目录号 192627B）并获得细胞和培养基的折扣价。 该试剂盒由冷冻保存的成人角质形成细胞安瓿（货号 192627）和 KGMTM-Gold BulletKitTM 生长培养基（货号 192060）组成
Content & Storage
Content
1 x KBMTM GoldTM Basal Medium (00192151), 500 mL
1 x KGMTMGoldTM SingleQuotsTM Supplement pack (00192152) containing:
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Natural Cap Vial with Transferrin, 0.50 mL
1 x Amber Vial with Epinephrine, 0.25 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
Related Products
KBM™ Gold Keratinocyte Growth Basal Medium 00192151
KBM™ Gold 角质形成细胞生长基础培养基 00192151
KGM™ Gold Keratinocyte Growth Medium SingleQuots™ Supplements and Growth Factors 00192152
KGM™ Gold 角质形成细胞生长培养基 SingleQuots™ 补充剂和生长因子 00192152
Data
Morphology of Lonza’s Adult Keratinocytes Grown in KGM™ Gold Growth Medium Compared to Competitor A’s Adult Keratinocytes in Their Medium.
The growth performance and longevity of Competitor A’s adult keratinocytes grown in Competitor A’s Medium were compared with Lonza’s adult keratinocytes grown in KGM-Gold™ Growth Medium. Although Competitor A claims ≥25 total population doublings for their adult keratinocytes, actual results show that this lot barely obtained 19 PD. Note the presence of several enlarged cells in Passage 2 in Competitor’s Medium. At the end of passage 4 (15 PD) Lonza’s adult keratinocytes consist of mostly small proliferative cells.
将竞争对手 A 的成年角质形成细胞在竞争对手 A 的培养基中生长的生长性能和寿命与龙沙在 KGM-Gold™ 生长培养基中生长的成年角质形成细胞进行了比较。 尽管竞争对手 A 声称其成年角质形成细胞的总种群倍增 ≥ 25，但实际结果表明，该批次几乎没有获得 19 个 PD。 注意竞争对手培养基中第 2 段中存在几个扩大的细胞。 在第 4 代（15 PD）结束时，Lonza 的成人角质形成细胞主要由小的增殖细胞组成。
lonza,CC-3182,CC-3181,00194850,CC-2576,SmGM™- 2 平滑肌细胞生长培养基 -2 BulletKit™
货号: CC-3182
Culture system containing SmBMTM Basal Medium (CC-3181) and SmGMTM-2 SingleQuotsTM supplements (CC-4149) required for growth of Smooth Muscle Cells
包含平滑肌细胞生长所需的 SmBMTM 基础培养基 (CC-3181) 和 SmGMTM-2 SingleQuotsTM 补充剂 (CC-4149) 的培养系统
Product Overview
SmGM-2TM BulletkitTM is also sold as separate basal medium and supplements
SmGMTM-2 Smooth Muscle Growth Medium-2 is a robust medium that was developed for the maintenance and expansion of human smooth muscle cells from various tissue origins including cardiac, airway, prostate and more. This kit is also utilized to support the maintenance of intestinal cells. SmGMTM-2 medium contains 5% FBS.
CloneticsTM Media Products are specially designed to support the growth of human and animal primary derived cells. Media systems have been specifically developed and optimized for various cell systems.
SmGM-2TM BulletkitTM 也作为单独的基础培养基和补充剂出售
SmGMTM-2 Smooth Muscle Growth Medium-2 是一种强大的培养基，用于维持和扩增来自各种组织来源的人类平滑肌细胞，包括心脏、气道、前列腺等。 该试剂盒还用于支持肠道细胞的维持。 SmGMTM-2 培养基含有 5% FBS。
CloneticsTM 培养基产品专为支持人和动物原代细胞的生长而设计。 媒体系统已针对各种细胞系统专门开发和优化。
Content & Storage
Content
1 x SmBMTM Basal Medium (CC-3181), 500 mL
1 x SmGMTM -2 SingleQuotsTM Supplement Pack (CC-4149) containing:
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Gray Cap Vial with hFGF-B, 1.00 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Bottle FBS, 25.00 mL
1 x Green Cap Vial with hEGF, 0.50 mL
Related Products
SmBM™ Smooth Muscle Cell Growth Basal Medium, 500 mL CC-3181
SmBM™ 平滑肌细胞生长基础培养基，500 mL CC-3181
SmGM™- 2 Smooth Muscle Cell Growth Medium-2 SingleQuots™ Supplements and Growth Factors CC-4149
D-BSMC-As – Diseased Human Bronchial Smooth Muscle Cells – Asthma 00194850
BdSMC – Human Bladder Smooth Muscle Cells CC-2533
D-BSMC-COPD – Diseased Human Bronchial Smooth Muscle Cells – COPD 00195274
UtSMC – Human Uterine Smooth Muscle Cells CC-2562
BSMC – Human Bronchial Smooth Muscle Cells CC-2576
AoSMC – Human Aortic Smooth Muscle Cells CC-2571
UASMC – Human Umbilical Artery Smooth Muscle Cells CC-2579
CASMC – Human Coronary Artery Smooth Muscle Cells CC-2583
PrSMC – Human Prostate Smooth Muscle Cells CC-2587
PrSMC – 人前列腺平滑肌细胞 CC-2587
InMyoFib – Human Intestinal Myofibroblasts CC-2902
InMyoFib – 人类肠道肌成纤维细胞 CC-2902
D-PASMC – Diseased Human Pulmonary Artery Smooth Muscle Cells – Diabetes Type II CC-2913
D-PASMC – 患病的人肺动脉平滑肌细胞 – II 型糖尿病 CC-2913
D-AoSMC – Diseased Human Aortic Smooth Muscle Cells – Diabetes Type I CC-2914
D-AoSMC – 患病的人主动脉平滑肌细胞 – I 型糖尿病 CC-2914
D-PASMC – Diseased Human Pulmonary Artery Smooth Muscle Cells – Diabetes Type I CC-2915
D-PASMC – 患病的人肺动脉平滑肌细胞 – I 型糖尿病 CC-2915
D-AoSMC – Diseased Human Aortic Smooth Muscle Cells – Diabetes Type II CC-2916
D-AoSMC – 患病的人主动脉平滑肌细胞 – II 型糖尿病 CC-2916
lonza,lonza CC-2512,CC-3132,CC-2547,CC-2547S,NHLF-人肺成纤维细胞
Catalog #: CC-2512
Cryopreserved ampule of Normal Human Lung Fibroblasts (NHLF) containing ≥ 500,000 cells
Product Overview
Lonza’s Human Lung Fibroblasts are isolated from adult lung tissue. Some matched donor sets of lung fibroblasts, bronchial smooth muscle cells, bronchial epithelial cells may be available. Contact Customer Service or Scientific Support for more information.
Lung fibroblasts play a role in airway inflammation and airway remodeling. They can be a target for asthma medications in pharmaceutical development. The most abundant cell type in lung interstitium is fibroblasts. They resemble ordinary fibroblasts but have some distinguishing features, for example, they have long branching processes and gap junctions. Their principle function is production of type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa.
Pulmonary fibroblasts (PF) play an important role in the repair and remodeling processes following injury. The controlled accumulation of fibroblasts to sites of inflammation is crucial to effective tissue repair after injury. Either inadequate or excessive accumulation of fibroblasts could result in abnormal tissue function. For example, the excess proliferation of fibroblasts contributes to the adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension. Important research applications includ pulmonary disease, i.e. COPD, asthma, CF, TB, cancer.
Cryopreserved CloneticsTM Normal Human Lung Fibroblasts cells are guaranteed through 15 population doublings, they are tested for negative von Willebrand Factor Expression/Factor VIII, cytokeratins 18 and 19, and alpha smooth muscle actin All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Purchase our Lung Fibroblast Cell Culture Kit (catalog no. CC-2512B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of lung fibroblast cells (catalog no. CC-2512) and FGMTM-2 BulletKitTM Growth Media (catalog no. CC-3132)
Lonza 的人肺成纤维细胞是从成人肺组织中分离出来的。一些匹配的肺成纤维细胞、支气管平滑肌细胞、支气管上皮细胞的供体组可能是可用的。有关更多信息，请联系客户服务或科学支持。
肺成纤维细胞在气道炎症和气道重塑中发挥作用。它们可以成为药物开发中哮喘药物的目标。肺间质中最丰富的细胞类型是成纤维细胞。它们类似于普通的成纤维细胞，但具有一些显着特征，例如，它们具有长分支过程和间隙连接。它们的主要功能是产生肺泡隔细胞外基质的 III 型胶原蛋白、弹性蛋白和蛋白聚糖。
肺成纤维细胞 (PF) 在损伤后的修复和重塑过程中发挥重要作用。成纤维细胞在炎症部位的受控积累对于损伤后的有效组织修复至关重要。成纤维细胞的不充分或过度积累都可能导致组织功能异常。例如，成纤维细胞的过度增殖导致在缺氧诱导的肺动脉高压发展过程中观察到的外膜增厚。重要的研究应用包括肺部疾病，即 COPD、哮喘、CF、TB、癌症。
冷冻保存的 CloneticsTM 正常人肺成纤维细胞保证通过 15 次群体倍增，它们被测试为阴性 von Willebrand 因子表达/因子 VIII、细胞角蛋白 18 和 19，以及 α 平滑肌肌动蛋白 所有细胞的支原体、细菌、酵母和真菌测试均为阴性.并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。
购买我们的肺成纤维细胞培养试剂盒（目录号 CC-2512B）并获得细胞和培养基的折扣价。该试剂盒由冷冻保存的肺成纤维细胞安瓿（货号 CC-2512）和 FGMTM-2 BulletKitTM 生长培养基（货号 CC-3132）组成
Content & Storage
Content
1 x Cryopreserved ampule of NHLF containing ≥ 500,000 cells
Instructions, SDS, CoA
Related Products
SAEC – Human Small Airway Epithelial Cells CC-2547
SAEC – Small Airway Epithelial Cells for S-ALITM Small Airway Air-Liquid-Interface Medium CC-2547S
HMVEC-L – Human Lung Microvascular Endothelial Cells CC-2527
HMVEC-L – 人肺微血管内皮细胞 CC-2527
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540
NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞
FGM™-2 Fibroblast Growth Medium-2 BulletKit™ CC-3132
FGM™-2 成纤维细胞生长培养基-2 BulletKit™ CC-3132
Data
NHLF – Human Lung Fibroblasts
Immunohistochemistry staining of normal human lung fibroblasts (CC-2512) stained with primary antibody Anti-Vimentin AB24525 (AbCam) used at 1:5000 dilution. Secondary antibody used was Horse anti Mouse, DI 2488 VectorLabs, used at 1:200 dilution. Nuclei were stained with Hoechst at 1:10,000.
NHLF – Human Lung Fibroblasts
Immunohistochemistry staining of normal human lung fibroblasts (CC-2512) stained with primary antibody Anti-Vimentin AB24525 (AbCam) used at 1:5000 dilution. Secondary antibody used was , Goat anti Chicken Alexa 594, A11042 Invitrogen: Used at 1:200, Horse anti Mouse, DI 2488 VectorLabs: Used at 1:200 Nuclei were stained with Hoechst.
用 1:5000 稀释度使用的一抗抗波形蛋白 AB24525 (AbCam) 对正常人肺成纤维细胞 (CC-2512) 进行免疫组织化学染色。 使用的二抗是山羊抗鸡 Alexa 594、A11042 Invitrogen：以 1:200 使用，马抗小鼠、DI 2488 VectorLabs：以 1:200 使用 细胞核用 Hoechst 染色。
lonza,lonza CC-5012,BE02-034E,CC-5034,胰蛋白酶/EDTA 溶液
Catalog #: CC-5012
Trypsin/EDTA 0.025% solution for use in cell culture
Product Overview
Trypsin is a clear, colorless-yellow, highly active bovine enzyme preparation, used to remove cells from the culture vessel surface into suspension. It is generally used at a working concentration of 0.025%, prepared in a balanced salt solution, without calcium or magnesium at a pH of 7.6-7.8. After treatment, trypsin activity can be halted by adding a serum media mixture or a trypsin inhibitor (from soybean, for example) in serum-free systems.
Trypsin/EDTA is also a component of Lonza’s ReagentPackTM Subculture Reagents kit (CC-5034) which includes Trypsin Neutralizing reagent and HEPES buffer.
胰蛋白酶是一种透明、无色黄色、高活性的牛酶制剂，用于将细胞从培养容器表面去除成悬浮液。 它通常在 0.025% 的工作浓度下使用，在平衡盐溶液中制备，不含钙或镁，pH 为 7.6-7.8。 处理后，可以通过在无血清系统中添加血清培养基混合物或胰蛋白酶抑制剂（例如来自大豆）来停止胰蛋白酶活性。
胰蛋白酶/EDTA 也是 Lonza 的 ReagentPackTM Subculture Reagents 试剂盒 (CC-5034) 的一个组成部分，其中包括胰蛋白酶中和试剂和 HEPES 缓冲液。
Content & Storage
Content
1 x Bottle Trypsin/EDTA 100 mL
Related Products
TrypZeanTM recombinant Trypsin EDTA (1x) BE02-034E
TrypZeanTM 重组胰蛋白酶 EDTA (1x) BE02-034E
ReagentPack™ Subculture Reagents, 100 mL CC-5034
ReagentPack™ 继代培养试剂，100 mL CC-5034
lonza,CC-4147,CC-2505,CC-3202,EGMTM-2 MV 微血管内皮 SingleQuotsTM 试剂盒
Catalog #: CC-4147
EGMTM-2 MV Microvascular Endothelial Cell Growth Medium-2 SingleQuotsTM Kit contains all supplements and growth factors required for Microvascular Endothelial Cells (basal media not included)
EGMTM-2 MV 微血管内皮细胞生长培养基-2 SingleQuotsTM 试剂盒包含微血管内皮细胞所需的所有补充剂和生长因子（不包括基础培养基）
Product Overview
EGMTM -2 MV SingleQuotTM Kit are components sold separately and does not include the basal medium. These are part of the EGMTM -2 MV Microvascular Endothelial Cell Growth Medium BulletKitTM (Catalog Number CC-3202) which consists of basal medium and supplements packaged together. Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used to grow CloneticsTM Primary Cells.
EGMTM -2 MV SingleQuotTM 套件是单独出售的组件，不包括基础培养基。 这些是 EGMTM -2 MV 微血管内皮细胞生长培养基 BulletKitTM（目录号 CC-3202）的一部分，它由包装在一起的基础培养基和补充剂组成。 从多种内皮细胞生长培养基中进行选择，以轻松培养原代内皮细胞。 每种内皮细胞生长培养基都有其特殊的配方，并针对某些细胞类型进行了优化。 每种类型的内皮细胞生长培养基在用于培养 CloneticsTM 原代细胞时都能保证发挥作用。
The following Endothelial Cell Growth Medium BulletKitsTM are available:
- EGMTM-2 BulletKitTM (CC-3162) – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Contains 2% FBS and VEGF for rapid proliferation. Does not contain BBE.
- EGMTM BulletKitTM (CC-3124) – Optimized for HUVECs cat. no. CC-2517 and CC-2519, contains 2% FBS and BBE. Does not contain exogenous VEGF.
- EGMTM-Plus BulletKitTM (CC-5035)– Enhanced version of EGMTM Medium. Optimized for HUVECs cat. no. CC-2935. Contains 2% FBS and BBE. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGMTM Medium.
- EGMTM-2 MV BulletKitTM (CC-3202) – Coronary and Iliac artery, human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. Does not contain BBE.
Lonza guarantees the performance of CloneticsTM/PoieticsTM Cells only if appropriate CloneticsTM/PoieticsTM Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.
只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
Content & Storage
Content
1 x Bottle FBS 25.00 mL
1 x Natural Cap Vial with Hydrocortisone 0.20 mL
1 x Gray Cap Vial with hFGF-B 2.00 mL
1 x White Cap Vial with VEGF 0.50 mL
1 x Yellow Cap Vial with R3-IGF-1 0.50 mL
1 x Blue Cap Vial with Ascorbic Acid 0.50 mL
1 x Green Cap Vial with hEGF 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL
Related Products
EGMTM -2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM CC-3202
EGMTM -2 MV 微血管内皮细胞生长培养基-2 BulletKitTM CC-3202
HMVEC-dNeo – Human Dermal Microvascular Endothelial Cells, Neonatal, Single Donor CC-2505
HMVEC-dNeo – 人真皮微血管内皮细胞，新生儿，单供体 CC-2505
HMVEC-L – Human Lung Microvascular Endothelial Cells CC-2527
HMVEC-L – 人肺微血管内皮细胞 CC-2527
HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult CC-2543
D-HCAEC – Diseased Human Coronary Artery Endothelial Cells – Diabetes Type I CC-2921
D-HCAEC – 患病的人冠状动脉内皮细胞 – I 型糖尿病 CC-2921
HIAEC – Human Iliac Artery Endothelial Cells CC-2545
HCAEC – Human Coronary Artery Endothelial Cells CC-2585
HCAEC – 人冠状动脉内皮细胞 CC-2585
D-HCAEC – Diseased Human Coronary Artery Endothelial Cells – Diabetes Type II CC-2922
D-HCAEC – 患病的人类冠状动脉内皮细胞 – II 型糖尿病 CC-2922
D-HMVEC-dAD – Diseased Human Dermal Microvascular Endothelial Cells, Adult – Diabetes Type I CC-2929
D-HMVEC-C – Diseased Human Cardiac Microvascular Endothelial Cells – Diabetes Type II CC-2928
D-HMVEC-C – 患病的人类心脏微血管内皮细胞 – II 型糖尿病 CC-2928
D-HMVEC-C – Diseased Human Cardiac Microvascular Endothelial Cells – Diabetes Type I CC-2927
D-HMVEC-C – 患病的人类心脏微血管内皮细胞 – I 型糖尿病 CC-2927
HMVEC-Bd – Human Bladder Microvascular Endothelial Cells CC-7016
HMVEC-Bd – 人膀胱微血管内皮细胞 CC-7016
HMVEC-C – Human Cardiac Microvascular Endothelial Cells CC-7030
HMVEC-C – 人心脏微血管内皮细胞 CC-7030
D-HMVEC-dAD – Diseased Human Dermal Microvascular Endothelial Cells, Adult – Diabetes Type II CC-2930
D-HMVEC-dAD – 患病的人类皮肤微血管内皮细胞，成人 – II 型糖尿病 CC-2930
lonza,CC-3190,CC-3191,REGM™ 肾上皮细胞生长培养基 BulletKit™
Catalog #: CC-3190
Culture system containing REBMTM Basal Medium (CC-3191) and REGMTM SingleQuotsTM supplements (CC-4127) required for growth of Renal Epithelial Cells.
培养系统包含肾上皮细胞生长所需的 REBMTM 基础培养基 (CC-3191) 和 REGMTM SingleQuotsTM 补充剂 (CC-4127)。
Product Overview
REGMTM Renal Epithelial Cell Growth Medium is formulated to maintain and expand most renal epithelial cells. REGMTM contains 0.5% serum.
REGMTM 肾上皮细胞生长培养基用于维持和扩增大多数肾上皮细胞。 REGMTM 含有 0.5% 的血清。
Select References:
- Diabetic Nephropathy is a chronic, progressive renal disease/diabetic kidney disease that develops in approximately one third of people with diabetes. In a study, Lonza’s human proximal tubular epithelial cells were cultured in respective media (REGMTM). The group investigated the role of KLK1 (kallikrein) in tubular pro-inflammatory responses in cultured human RPTEC and examined the role of PAR-4 (Protease-Activated Receptor 4) activation in KLK1-mediated signaling in the development of DN. Yiu WH, Wong DW, Chan LY, Leung JC, Chan KW, Lan HY, Lai KN, Tang SC. Tissue Kallikrein Mediates Pro-Inflammatory Pathways and Activation of Protease-Activated Receptor-4 in Proximal Tubular Epithelial Cells. PLoS ONE (2014); 9(2): e88894
- Renal Graft Research: In vitro renal epithelial cell culture models have provided a great deal of information about the outcome of renal clinical transplantation models. In a study carried out using Lonza’s Primary Human Renal Proximal Tubular Epithelial cells maintained in REGMTMTM renal epithelial growth media (cat# CC-2553 & CC-3190 respectively), the group study the effects of HCMV (Human cytomegalovirus) infection on renal cells. Masako Shimamura, Joanne E. Murphy-Ullrich and William J. Britt. Human Cytomegalovirus Induces TGF-β1 Activation in Renal Tubular Epithelial Cells after Epithelial-to-Mesenchymal Transition. PLoS Pathogens. (2010); 6(11): e1001170
Content & Storage
Content
1 x REBMTM Basal Medium (CC-3191), 500 mL
1 x REGMTM SinglequotsTM Supplement Pack (CC-4127) containing:
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Bottle FBS, 2.50 mL
1 x Natural Cap Vial with Transferrin, 0.50 mL
1 x Amber Vial with Triiodothyronine, 0.50 mL
1 x Amber Vial with Epinephrine, 0.50 mL
Related Products
REBM™ Renal Epithelial Cell Growth Basal Medium CC-3191
REBM™ 肾上皮细胞生长基础培养基 CC-3191
REGMTM Renal Epithelial Cell Growth Medium SingleQuotsTM Kit CC-4127
REGMTM 肾上皮细胞生长培养基 SingleQuotsTM 试剂盒 CC-4127
lonza,CC-2511,原代细胞和干细胞,NHDF-Ad – 人皮肤成纤维细胞
Catalog #: CC-2511
Cryopreserved ampule of Normal Adult Human Dermal Fibroblasts (NHDF-Ad) containing ≥ 500,000 cells
Product Overview
Human Dermal Fibroblasts are available from either adult skin (NHDF-Ad) or neonatal foreskin (NHDF-Neo). They are derived from the dermis of normal human neonatal foreskin or adult skin. They are cryopreserved at the end of primary culture. Normal Human Epidermal Keratinocytes (00192627) from matched donors are available for certain lots
Lonza’s human dermal fibroblasts are guaranteed through 15 population doublings when using FGMTM-2 Growth Media. Dermal fibroblasts are characterized by morphological observation throughout serial passage.
All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
人类皮肤成纤维细胞可从成人皮肤 (NHDF-Ad) 或新生儿包皮 (NHDF-Neo) 中获得。 它们来源于正常人类新生儿包皮或成人皮肤的真皮。 它们在原代培养结束时被冷冻保存。 来自匹配供体的正常人表皮角质形成细胞 (00192627) 可用于某些批次
使用 FGMTM-2 生长培养基时，Lonza 的人类真皮成纤维细胞可通过 15 倍的种群倍增得到保证。 真皮成纤维细胞的特征在于贯穿连续传代的形态观察。
所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。
Applications
- ECM protein analysis
- Wound healing
- Collagen metabolism
- Cosmetics
- Skin therapy/models
Content & Storage
Content
1 x Cryopreserved ampule of NHDF-Ad containing ≥ 500,000 cells
Related Products
HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult CC-2543
NHDF-Neo – Human Dermal Fibroblasts, Neonatal CC-2509
NHEK-Ad – Human Epidermal Keratinocytes, Adult, Single Donor 00192627
NHEM-Ad – Human Epidermal Melanocytes, Adult CC-2586
FGM™-2 Fibroblast Growth Medium-2 BulletKit™ CC-3132
P2 Primary Cell 4D-Nucleofector™ X Kit L V4XP-2012
P2 Primary Cell 4D-Nucleofector™ X Kit L V4XP-2024
P2 Primary Cell 4D-Nucleofector™ X Kit S V4XP-2032
P2 Primary Cell 96-well Nucleofector™ Kit V4SP-2096
P2 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-2960
P2 Primary Cell 384-well Nucleofector™ Kit V5SP-2002
P2 Primary Cell 384-well Nucleofector™ Kit V5SP-2010
NHDF-Ad – Human Dermal Fibroblasts, Adult
Immunohistochemistry staining of normal human adult dermal fibroblasts (CC-2511) epanded into a 24 well plate. Cells were stained with primary antibody Anti-Vimentin AB24525 (AbCam) and secondary antibody Alexa 594, Goat anti Chicken, A11042 Invitrogen was used at 1:200 dilution.
lonza,lonza CC-2540,NHBE-含有维甲酸的人支气管/气管上皮细胞
Catalog #: CC-2540
Cryopreserved ampule of Normal Human Bronchial Epithelial (NHBE) cells with Retinoic Acid containing ≥500,000 cells
Product Overview
Normal and Diseased Bronchial Epithelial (NHBE and DHBE) cells are isolated from epithelial lining of airways above bifurcation of the lungs. Lonza’s Normal Human Bronchial Epithelial Cells (NHBE) are available with retinoic acid.
All Cryopreserved human bronchial epithelial cells are guaranteed through 15 population doublings. Cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
正常和患病的支气管上皮（NHBE 和 DHBE）细胞是从肺分叉上方的气道上皮衬里分离出来的。 Lonza 的正常人支气管上皮细胞 (NHBE) 可与视黄酸一起使用。
所有冷冻保存的人支气管上皮细胞通过 15 次群体倍增得到保证。 细胞对支原体、细菌、酵母和真菌检测呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。
Select Applications with Lonza’s Human Bronchial Epithelial Cells –
- A Rotating Bioreactor for Scalable Culture and Differentiation of Respiratory Epithelium”. Respiratory epithelium is difficult to grow as it requires a well-maintained polarizing air‐liquid interface (ALI) to maintain differentiation. Raredon et al. describe a scalable and easy method to conduct air-liquid interface with Lonza’s bronchial epithelial cells guaranteed for ALI differentiation (Cat. No. CC-2540S).
- Cell concentration and space for growth are required for productive NHBE cell branching in vitro. Click to read this article by Hagiwara et.al that shows how co-cultures with HUVECs in 3D culture influence reconstruction of branched tubular structures in lung epithelial cells.
- Wu, et al. reports for the first time that HBE cells when grown in B-ALITMMedium can differentiate into 3D glandular acinar structures, likesalivary and mammary epithelial. The cells were grown on the basement membrane matrix Matrigel under select conditions and have shown to exhibit formation of brochospheres or tracheospheres with ciliated lining.
Purchase our Bronchial Epithelial Cell Culture Kit (catalog no. CC-2540B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of bronchial epithelial cells (catalog no. CC-2540) and BEGMTMTM BulletKitTM Growth Media (catalog no. CC-3170)
Benefits
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis provided for every cell lot
Content & Storage
Content
1 x Cryopreserved ampule NHBE w/ RA containing ≥500,000 cells
Related Products
NHBE – Human Bronchial/Tracheal Epithelial Cells without Retinoic Acid CC-2541
D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma for B-ALI™ Culture 00194911S
D-HBE-COPD – Diseased Human Bronchial/Tracheal Epithelial Cells – COPD for B-ALITM 00195275S
B-ALI™ Bronchial Air-Liquid Interface Medium BulletKit™ 00193514
Bronchial Epithelial Cells in RAFT™ 3D Cell Culture System
Bronchial Epithelial Cells Grown in BEGM™ BulletKit™ and Cultured in RAFT™ 3D Cell Culture System
Normal Human Bronchial Epithelial Cell
Normal human bronchial epithelial cell (NHBE) culture showing excellent packed cuboidal morphology
lonza,lonza CC-3151,CC-3153,MEBM™ 乳腺上皮细胞生长基础培养基
Catalog #: CC-3151
MEBMTM Mammary Epithelial Basal Medium, for culture of human mammary epithelial cells, 500 mL
Product Overview
MEBMTM is a mammary epithelial cell basal medium sold separately. This can be used with 1 kit of CC-4136 (mammary epithelial cell growth medium SingleQuotsTM Supplements and Growth Factors). The full MEGMTMTM BulletKitTM consisting of basal medium and supplements can be purchased together using the catalog number CC-3150.
MEGMTMTM Mammary Epithelial Cell Growth Medium BulleKitTM has been optimized for the growth of Mammary Epithelial cells in a serum-free environment. This media system has been referenced in over 130 unique publications primarily focusing on breast cancer research.
MEBMTM 是一种乳腺上皮细胞基础培养基，单独出售。 这可以与 1 套 CC-4136（乳腺上皮细胞生长培养基 SingleQuotsTM 补充剂和生长因子）一起使用。 由基础培养基和补充剂组成的完整 MEGMTMTM BulletKitTM 可以使用目录号 CC-3150 一起购买。
MEGMTMTM 乳腺上皮细胞生长培养基 BulleKitTM 针对乳腺上皮细胞在无血清环境中的生长进行了优化。 该媒体系统已被 130 多种主要关注乳腺癌研究的独特出版物引用。
Content & Storage
Content
1 x Bottle Basal Medium 500 mL
Instructions, SDS, CoA
Related Products
MEBM™ Mammary Epithelial Cell Growth Basal Medium, Phenol Red Free CC-3153
MEBM™ 乳腺上皮细胞生长基础培养基，无酚红 CC-3153
Data
Lonza’s HMEC cells cultured in MEGM™ BulletKit™ Medium vs. other commercially available systems
Graph showing the propagation potential of Lonza’s HMEC cells cultured in MEGM™ BulletKit™ Medium vs. other commercially available systems
A Three-Dimensional RAFT™ Co-Culture as Advanced Model for Breast Cancer Drug Discovery
Presented at AACR 2017, we demonstrate here the feasibility of co-culturing human mammary fibroblasts and human mammary epithelial cells in RAFT™ 3D Culture System as a 3D breast cancer model.
lonza,lonza CC-2527,HMVEC-L – 人肺微血管内皮细胞
Catalog #: CC-2527
Cryopreserved ampule of Human Lung Microvascular Endothelial Cells (HMVEC-L) containing ≥ 500,000 cells
Interested in reducing lab time? Lonza offers proliferating primary cells in a variety of convenient flask and plate formats.
有兴趣减少实验室时间吗？ Lonza 以各种方便的烧瓶和板形式提供增殖原代细胞
Product Overview
Lonza’s Lung Microvascular Endothelial Cells include cells isolated from small vessels within normal lung tissue that are cultured to be ≥90% pure and specifically enriched for lung LECs and BECs. Lung microvascular cells, CC-2527 (HMVEC-L), are a mixed population of both blood (HMVEC-LBl) and lymphatic (HMVEC-LLy) endothelial cells.
Additional donor characteristics including hypertension, or smoking history may be available by contacting Scientific Support.
Cryopreserved CloneticsTM Lung Microvascular Endothelial Cells are guaranteed through 15 population doublings, express CD31/105, von Williebrand Factor VIII, are positive for acetyated low density lipoprotein uptake, and are PECAM positive. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Lonza 的肺微血管内皮细胞包括从正常肺组织内的小血管中分离出来的细胞，这些细胞经培养后纯度≥90%，并特别富含肺 LEC 和 BEC。 肺微血管细胞 CC-2527 (HMVEC-L) 是血液 (HMVEC-LB1) 和淋巴 (HMVEC-LLy) 内皮细胞的混合群体。
联系科学支持可以获取其他供体特征，包括高血压或吸烟史。
冷冻保存的 CloneticsTM 肺微血管内皮细胞保证通过 15 次群体倍增，表达 CD31/105，von Williebrand 因子 VIII，对乙酰化低密度脂蛋白摄取呈阳性，并且是 PECAM 阳性。 所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。
Benefits
- Test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- A Certificate of Analysis is provided for each cell lot purchased
Applications
- Oncology
- Tumorigenesis
- Drug Development
- Circulatory Physiology
- Wound Healing
- Lymphangiogenesis
- Immunology
- Basic Research
Content & Storage
Content
1 x Cryopreserved ampule of HMVEC-L containing ≥ 500,000 cells
Related Products
SAEC – Small Airway Epithelial Cells for S-ALITM Small Airway Air-Liquid-Interface Medium CC-2547S
SAEC – 用于 S-ALITM 小气道气液界面培养基 CC-2547S 的小气道上皮细胞
NHLF – Human Lung Fibroblasts CC-2512
BSMC – Human Bronchial Smooth Muscle Cells CC-2576
BSMC – 人支气管平滑肌细胞 CC-2576
NHBE – Human Bronchial/Tracheal Epithelial Cells for B-ALITM Culture CC-2540S
NHBE – 用于 B-ALITM 培养的人支气管/气管上皮细胞 CC-2540S
EGMTM -2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM CC-3202
EGMTM -2 MV 微血管内皮细胞生长培养基-2 BulletKitTM CC-3202
P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5012
P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5024
P5 Primary Cell 4D-Nucleofector™ X Kit S V4XP-5032
P5 Primary Cell 96-well Nucleofector™ Kit V4SP-5096
P5 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-5096
P5 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-5960
P5 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-5960
P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5002
P5 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-5002
P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5010
P5 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-5010
lonza,lonza CC-3171,BEBM™ 支气管上皮细胞生长基础培养基
Catalog #: CC-3171
BEBMTM Bronchial Epithelial Basal Medium. Available for immediate shipment.
Product Overview
BEBMTM is a basal medium sold separately. This is used as a component in the BEGMTMTM Bronchial Epithelial Cell Growth Medium BulletKitTM (catalog number CC-3170) which consists of basal medium and supplements packaged together.
BEGMTMTM Bronchial Epithelial Cell Growth Medium is specially designed to support the growth of human primary bronchial epithelial cells. BEGMTMTM BulletKitTM is serum-free and has been optimized to grow cells from normal and diseased tissues.
Published research shows BEGMTMTM BulletKitTM has expanded applications. Our customers have used BEGMTMTM BulletKitTM to culture well-established cell lines such as BEAS-2B, home-grown bronchial epithelial cells and supporting co-culture models with breast cancer cell lines such as MCF-7 and MDA-MB-231.
BEGMTM is an optimized and readily available media kit providing researchers the confidence and the convenience of a system that works to support their cells. This is especially important for researchers who are utilizing cell lines as a stepping stone to primary cell culture. Working with the same BEGMTM Media between cell lines and primary NHBE can reduce variability within the experiments and simplify the shift for researchers. In addition, if primary cells are utilized as a side-by-side control with normal cell lines or home-grown bronchial epithelial cells, the BEGMTMTM BulletKitTM Media may also offer cost-benefit since customers can use the same media to support multiple cell types.
BEBMTM 是一种单独出售的基础培养基。这用作 BEGMTMTM 支气管上皮细胞生长培养基 BulletKitTM（目录号 CC-3170）的一个成分，该培养基由包装在一起的基础培养基和补充剂组成。
BEGMTMTM 支气管上皮细胞生长培养基专为支持人原代支气管上皮细胞的生长而设计。 BEGMTMTM BulletKitTM 不含血清，经过优化，可从正常和患病组织中培养细胞。
已发表的研究表明 BEGMTMTM BulletKitTM 已扩展应用。我们的客户已使用 BEGMTMTM BulletKitTM 培养成熟的细胞系，如 BEAS-2B、本土支气管上皮细胞，并支持与乳腺癌细胞系如 MCF-7 和 MDA-MB-231 的共培养模型。
BEGMTM 是一种经过优化且随时可用的培养基套件，可为研究人员提供支持其细胞的系统的信心和便利性。这对于利用细胞系作为原代细胞培养垫脚石的研究人员来说尤其重要。在细胞系和原代 NHBE 之间使用相同的 BEGMTM 培养基可以减少实验中的变异性并简化研究人员的转变。此外，如果将原代细胞与正常细胞系或自生支气管上皮细胞一起用作对照，BEGMTMTM BulletKitTM 培养基还可以提供成本效益，因为客户可以使用相同的培养基来支持多种细胞类型.
Content & Storage
Content
1 x Bottle Basal Medium 500 mL
Instructions, SDS, CoA
Related Products
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540
NHBE – Human Bronchial/Tracheal Epithelial Cells without Retinoic Acid CC-2541
D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma 00194911
D-HBE-COPD – Diseased Human Bronchial/Tracheal Epithelial Cells – COPD 00195275
D-HBE-COPD – 患病的人支气管/气管上皮细胞 – COPD 00195275
BEGM™ Bronchial Epithelial Cell Growth Medium BulletKit™ CC-3170
BEGM™ 支气管上皮细胞生长培养基 BulletKit™ CC-3170
lonza,CC-2547,V5SP-2010,SAEC – 人类小气道上皮细胞
Catalog #: CC-2547
Cryopreserved ampule of Small Airway Epithelial Cells (SAEC) containing ≥500,000 cells.
Product Overview
Lonza’s Human Small Airway Epithelial Cells (SAEC) are isolated from normal human lung tissue the distal portion of the lung in the 1mm bronchiole area.
Cryopreserved SAECs or Alveolar Cells are guaranteed through 15 population doublings and stain positive for cytokeratin 19. SAECs or Alveolar Cells labeled CC-2547S are suitable for air-liquid interface studies and are guaranteed for differentiation marked by cilia formation, mucin production, and TEER levels. Cells from various diseased donors are also available.
All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Lonza 的人小气道上皮细胞 (SAEC) 是从正常人肺组织中分离出来的，位于 1mm 细支气管区域的肺远端部分。
冷冻保存的 SAEC 或肺泡细胞通过 15 次群体倍增和染色呈细胞角蛋白阳性 19 得到保证。标记为 CC-2547S 的 SAEC 或肺泡细胞适用于气液界面研究，并保证以纤毛形成、粘蛋白产生和 TEER 水平为标志的分化 . 还可以获得来自各种患病供体的细胞。
所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。
Benefits
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis provided for every cell lot
Content & Storage
Content
1 x Cryopreserved ampule SAEC containing ≥500,000 cells
Related Products
SAEC – Small Airway Epithelial Cells for S-ALITM Small Airway Air-Liquid-Interface Medium CC-2547S
SAEC – 用于 S-ALITM 小气道气液界面培养基 CC-2547S 的小气道上皮细胞
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540
NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞
DHLF-IPF – Diseased Human Lung Fibroblasts, Idiopathic Pulmonary Fibrosis CC-7231
SAGM™ Small Airway Epithelial Cell Growth Medium BulletKit™ CC-3118
SAGM™ 小气道上皮细胞生长培养基 BulletKit™ CC-3118
P2 Primary Cell 4D-Nucleofector™ X Kit L V4XP-2012
P2 Primary Cell 4D-Nucleofector™ X Kit L V4XP-2024
P2 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-2024
P2 Primary Cell 4D-Nucleofector™ X Kit S V4XP-2032
P2 Primary Cell 96-well Nucleofector™ Kit V4SP-2096
P2 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-2960
P2 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-2960
P2 Primary Cell 384-well Nucleofector™ Kit V5SP-2002
P2 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-2002
P2 Primary Cell 384-well Nucleofector™ Kit V5SP-2010
P2 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-2010
Data
Co-culture Experiments with Lonza’s Small Airway Epithelial Cells and SAGM™ BulletKit™ Media
Data Courtesy of Furukawa, et al. Lonza’s SAEC cells were co-cultured with primary normal mammary epithelial cells, breast cancer cell lines MCF-7 and MDA-MB-231 in Lonza’s SAGM™ BulletKit™ Growth Media. Breast cancer cell lines showed differences in morphology and proliferation rates in the presence of small airway epithelial cells. The image above shows phase contrast imaging with a fluorescent (red) overlay of MCF-7 cell line. MCF-7 cell lines survived as tight clusters in SAGM™ Media. When co-cultured with Lonza’s primary SAECs, MCF-7 cells demonstrated enhanced growth all the while staying clustered.
lonza,HUCPI,Human Hepatocytes, Cryopreserved, Plateable HUCPG
Catalog #: HUCPI
Cryopreserved Human Hepatocytes, Plateable and Interaction Qualified are characterized for 3 major mechanisms of Drug-Drug interactions: transporter activity, enzyme activity, and induction potential, providing you with one product to meet more of your hepatocyte DDI study needs Each ampule contains >5 million cells.
冷冻保存的人类肝细胞、可铺板和相互作用认证具有药物-药物相互作用的 3 种主要机制的特征：转运蛋白活性、酶活性和诱导潜力，为您提供一种产品来满足您更多的肝细胞 DDI 研究需求每个安瓿含有 >500 万 细胞。
Product Overview
临床相关的药物-药物相互作用 (DDI) 是任何新药开发项目的一个严重问题。
To better support capturing the multiple mechanisms of DDI potential using cryopreserved primary human hepatocytes, we now offer Interaction Qualified Cryopreserved Human Hepatocytes (Catalog HUCPI) which are characterized for 3 major mechanisms of DDI:
- Transporter activity
- Enzyme activity
- Enzyme gene induction potential.
Interaction Qualified Cryopreserved Human Hepatocytes have the following features and benefits:
Benefits
- Use the same donor for basal clearance, transport, and induction studies
- Actual rates of transport reported rather than relative rates for less ambiguity
- Large lots mean fewer rounds of testing so you can focus on results
- Prequalified in Lonza media for better reproducibility
Features*:
- Confluent monolayer with cobblestone morphology, distinct nuclei, and tight junctions for 5 days or more
- Inducibility of enzyme activity for CYP3A4, CYP2B6, and CYP1A2
- Inducibility of mRNA for CYP3A4, CYP2B6, CYP1A2, and CYP2C8 genes
- Actual rate of uptake or efflux for OATP1B1/3, OCT1/2, NTCP, and BSEP** transporters
- Differences in passive vs. active uptake for OATP1B1/3, OCT1/2, and NTCP transporters.
- Basal metabolism for 8 CYPs, SULT, UGT, and aldehyde oxidase
- Plated low-turnover clearance for CYP2C9, CYP2D6, and CYP3A4
- Complementary thawing, plating, and maintenance medium available
- > 5 million viable cells/vial
- Characterized for long-term culture potential (> 7 days)
- Characterized for spheroid formation potential
Our qualification methods for cryopreserved human hepatocytes are modelled after recommendations from the FDA publication In Vitro Metabolism and Transporter Mediated Drug-Drug Interaction Studies Guidance for Industry (2017, http://www.fda.gov/Drugs/GuidanceComplianceRegulatoryInformation/Guidances/default.htm) .
*Currently, older batches may not have a complete data set of characteristics listed
***BSEP efflux activity is measured using method described in Jie Zhang, et al., Chemico-Biological Interactions, Volume 255, 2016, Pages 45-54,
Benefits
- Use the same donor for basal clearance, transport, and induction studies
- Actual rates of transport reported rather than relative rates for less ambiguity
- Large lots mean fewer rounds of testing so you can focus on results
- Prequalified in Lonza media for better reproducibility
- Pre-tested for long-term culture, spheroid formation, and 96-well compatibility
Applications
- Drug-Drug interaction studies
- Low clearance metabolism studies
- Drug uptake and efflux
- Disease modeling
- Xenotransplantation
- Short term cellular toxicity studies
- 3D cell culture
Content & Storage
Content
Cryopreserved ampule of HUCPI containing >5 million viable cells
Instructions, SDS, CoA
Related Products
Human Hepatocytes, Cryopreserved, Plateable HUCPG
Data
lonza,CC-4136,CC-3150,MEGMTM 乳腺上皮细胞生长培养基 SingleQuotsTM 试剂盒
货号: CC-4136
Supplements and growth factors required for the culture of Mammary Epithelial Cells (basal media M CC-3151 not included).
乳腺上皮细胞培养所需的补充剂和生长因子（不包括基础培养基 M CC-3151）。
Product Overview
MEGMTM SingleQuotTM Kit are components sold separately and does not include the basal medium. These are part of the MEGMTM Mammary Epithelial Cell Growth Medium BulletKitTM (Catalog Number CC-3150). The full MEGMTM BulletKitTM consisting of basal medium and supplements can be purchased together using the catalog number CC-3150.
Mammary Epithelial Cell Growth Medium BulletKitTM has been optimized for the growth of Mammary Epithelial cells in a serum-free environment. This media system has been referenced in over 130 unique publications primarily focusing on breast cancer research.
MEGMTM SingleQuotTM 试剂盒是单独出售的组件，不包括基础培养基。 这些是 MEGMTM 乳腺上皮细胞生长培养基 BulletKitTM（目录号 CC-3150）的一部分。 由基础培养基和补充剂组成的完整 MEGMTM BulletKitTM 可以使用目录号 CC-3150 一起购买。
乳腺上皮细胞生长培养基 BulletKitTM 针对乳腺上皮细胞在无血清环境中的生长进行了优化。 该媒体系统已被 130 多种主要关注乳腺癌研究的独特出版物引用。
Content & Storage
Content
1 x Orange Cap Vial with BPE 2.00 mL
1 x Green Cap Vial with hEGF 0.50 mL
1 x Lilac Cap Vial with Insulin 0.50 mL
1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL
Related Products
MEGM™ Mammary Epithelial Cell Growth Medium BulletKit™ CC-3150
MEGM™ 乳腺上皮细胞生长培养基 BulletKit™ CC-3150
Data
Lonza’s HMEC cells cultured in MEGM™ BulletKit™ Medium vs. other commercially available systems
Graph showing the propagation potential of Lonza’s HMEC cells cultured in MEGM™ BulletKit™ Medium vs. other commercially available systems
A Three-Dimensional RAFT™ Co-Culture as Advanced Model for Breast Cancer Drug Discovery
Presented at AACR 2017, we demonstrate here the feasibility of co-culturing human mammary fibroblasts and human mammary epithelial cells in RAFT™ 3D Culture System as a 3D breast cancer model.
lonza,CC-3186,R-HIAS-521,AGM™ 星形胶质细胞生长培养基 BulletKit™
Catalog #: CC-3186
Culture system containing ABMTM Basal Medium (CC-3187) and AGMTM SingleQuotsTM Supplements (CC-4123) required for growth of Astrocytes
Product Overview
CloneticsTM AGMTM Astrocyte Growth Medium has been developed to support the growth of primary astrocytes. Media products are provided as convenient BulletKitsTM which contain basal medium and necessary growth factors for cell culture.
CloneticsTM AGMTM 星形胶质细胞生长培养基用于支持原代星形胶质细胞的生长。 培养基产品以方便的 BulletKitsTM 形式提供，其中包含基础培养基和细胞培养所需的生长因子。
Media are supplied in several formats:
- Basal Medium – Contains no growth factors cytokines, or supplements
- SingleQuotsTM – Growth factors, cytokines, and supplements (basel media not included)
- BulletKitsTM – Basal media and SingleQuotsTM Kit packaged together
Content & Storage
Content
1 x ABMTM Basal Medium (CC-3187), 500 mL
1 x AGMTM SingleQuotsTM Supplement Pack (CC-4123) containing:
1 x Bottle FBS, 15.0 mL
1 x Bottle L-Glutamine, 5.00 mL
1 x Vial GA-1000, 0.50 mL
1 x Vial Ascorbic Acid, 0.50 mL
1 x Vial HEGF, 0.50 mL
1 x Vial Insulin, 1.25 mL
Related Products
ABM™ Astrocyte Basal Medium CC-3187
ABM™ 星形胶质细胞基础培养基 CC-3187
AGM™ Astrocyte Growth Medium SingleQuots™ Supplements and Growth Factors CC-4123
AGM™ 星形胶质细胞生长培养基 SingleQuots™ 补充剂和生长因子 CC-4123
NHA – Human Astrocytes CC-2565
Mouse C57 Brain Mixed Astrocytes M-ASM-330
Mouse CD1 Brain Mixed Astrocytes M-ASM-430
Rat Brain Cx-Hi-Cp Mixed Astrocytes R-ASM-530
大鼠脑 Cx-Hi-Cp 混合星形胶质细胞 R-ASM-530
Rat Brain Striatum Astrocytes R-CPAS-522
大鼠脑纹状体星形胶质细胞 R-CPAS-522
Rat Brain Cortex Astrocytes R-CXAS-520
大鼠脑皮质星形胶质细胞 R-CXAS-520
Rat Brain Hippocampus Astrocytes R-HIAS-521
大鼠脑海马星形胶质细胞 R-HIAS-521
lonza,lonza HUCPG,人肝细胞，冷冻保存,lonza HUCPI
Catalog #: HUCPG
冷冻保存的人类肝细胞，可电镀，经过测试，具有高活力和至少 5 天的健康培养持续时间。 每个安瓿含有 >500 万个活细胞。
Product Overview
Cryopreserved Human Hepatocytes, Plateable format are tested for high viability and healthy culture duration of at least 5 days in 2D sandwich culture. The General Purpose plateable hepatocytes are ideal for disease modeling, genomics applications, and other applications where high quality plated hepatocytes are required.
The General Purpose hepatocytes should not be used in drug-drug interaction studies. For drug-drug interaction studies, please refer to the Induction Qualified hepatocytes (HUCPI)
冷冻保存的人类肝细胞，可铺板形式在 2D 夹心培养中测试了高活力和至少 5 天的健康培养持续时间。 通用型可电镀肝细胞是疾病建模、基因组学应用和其他需要高质量电镀肝细胞的应用的理想选择。
通用肝细胞不应用于药物-药物相互作用研究。 对于药物-药物相互作用研究，请参阅诱导合格肝细胞 (HUCPI)
NEW!!
The General Purpose cryopreserved human hepatocytes, plateable are now verified for long-term plating, plating in 96-well format, and for spheroid fomation capacity.
Please download our recent inventory from the link below and look for our new Verified for Long TermTM, Verified for 96-wellTM and Verified for SpheroidsTM Characteristics to select your optimal batch.
Benefits
- Precharacterized for appropriate hepatocyte morphology
- Large inventory of multiple donors
- Use for 3D culture applications, look for Verified for SpheroidsTM Hepatocyte batches
- Tested for long-term applications and 96-well compatability
Applications
- Disease modeling
- Genomics
- Xenotransplantation
Content & Storage
Content
1 x Cryopreserved ampule HUCPG containing >5 million viable cells
Instructions, SDS, CoA
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Human Hepatocytes, Cryopreserved, Plateable and Interaction Qualified HUCPI
人肝细胞，冷冻保存，可电镀和相互作用合格的 HUCPI
lonza,lonza CC-3118,CC-4124,SAGM™ 小气道上皮细胞生长培养基 BulletKit™
Catalog #: CC-3118
Culture system containing SABMTM Basal Medium (CC-3119) and SAGMTM SingleQuotsTM supplements (CC-4124) required for growth of Small Airway Epithelial cells
Product Overview
SAGMTM BulletKitTM is small airway epithelial cell growth medium that is serum-free and has been optimized to grow human small airway epithelial cells (SAEC) from normal and diseased tissue.
Published research shows SAGMTMTM BulletKitTM Small Airway Epithelial Cell Growth Medium is also suitable to support co-culture experiments with primary mammary epithelial cells (HMEC) as well as established breast cancer cell lines such as MCF-7 and MDA-MB-231. Refer to the relevant published data in the ‘Data’ tab.
SAGMTM BulletKitTM 是无血清的小气道上皮细胞生长培养基，经过优化，可从正常和患病组织中培养人小气道上皮细胞 (SAEC)。
已发表的研究表明 SAGMTMTM BulletKitTM 小气道上皮细胞生长培养基也适用于支持与原代乳腺上皮细胞 (HMEC) 以及已建立的乳腺癌细胞系（如 MCF-7 和 MDA-MB-231）的共培养实验。 请参阅“数据”选项卡中的相关已发布数据。
Content & Storage
Content
1 x SABMTM Basal Medium (CC-3119), 500 mL
1 x SAGMTM SingleQuotsTM Supplement Pack (CC-4124) containing:
1 x Orange Cap Vial with BPE, 2.0 mL
1 x Lilac Cap Vial with Insulin, 0.5 mL
1 x Natural Cap Vial with Hydrocortisone, 0.5 mL
1 x Red Cap Vial with GA-1000, 0.5 mL
1 x Amber Vial with Retinoic Acid, 0.5 mL
1 x Bottle with Fatty Acid Free BSA, 5 mL
1 x Natural Cap Vial with Transferrin, 0.5 mL
1 x Amber Vial with Triiodothyronine, 0.5 mL
1 x Amber Vial with Epinephrine, 0.5 mL
1 x Green Cap Vial with hEGF, 0.5 mL
Related Products
SABM™ Small Airway Epithelial Cell Growth Basal Medium CC-3119
SABM™ 小气道上皮细胞生长基础培养基 CC-3119
SAGM™ Small Airway Epithelial Cell Growth Medium SingleQuots™ Supplements and Growth Factors CC-4124
SAGM™ 小气道上皮细胞生长培养基 SingleQuots™ 添加剂和生长因子 CC-4124
Co-culture Experiments with Lonza’s Small Airway Epithelial Cells and SAGM™ BulletKit™ Media
数据由 Furukawa 等人提供。 Lonza 的 SAEC 细胞与原代正常乳腺上皮细胞、乳腺癌细胞系 MCF-7 和 MDA-MB-231 在 Lonza 的 SAGM™ BulletKit™ 生长培养基中共培养。 乳腺癌细胞系在存在小气道上皮细胞的情况下表现出形态和增殖率的差异。 上图显示了 MCF-7 细胞系的荧光（红色）覆盖的相差成像。 MCF-7 细胞系在 SAGM™ 培养基中以紧密簇的形式存活。 当与 Lonza 的原代 SAEC 共培养时，MCF-7 细胞在保持聚集的同时表现出增强的生长。
lonza,00192627,NHEK-Ad – 人表皮角质形成细胞,成人,单一供体
Catalog #: 00192627
Cryopreserved Human Adult Epidermal Keratinocytes (NHEK-Ad) from a single donor, ≥ 500,000 cells
Product Overview
Cryopreserved Normal Human Epidermal Keratinocytes are guaranteed through 18 population doublings for adult cells. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Normal Human Dermal fibroblasts from matched donors are available for certain lots.
冷冻保存的正常人表皮角质形成细胞通过 18 次成体细胞的群体倍增得到保证。 所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。 来自匹配供体的正常人真皮成纤维细胞可用于某些批次。
Purchase our Adult Keratinocyte Cell Culture Kit (catalog no. 00192627B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of adult keratinocytes (catalog no. 00192627) and KGMTM-Gold BulletKitTM Growth Media (catalog no. 00192060)
References with Lonza’s Keratinocytes:
- Protective barrier: Keratinocytes were stimulated with histamine or histamine receptor ligands to show the formation of a defective skin barrier. Gschwandtner M, Mildner M, Mlitz V, Gruber F, Eckhart L, Werfel T, Gutzmer R, Elias PM and Tschachler E. Histamine suppresses epidermal keratinocyte differentiation and impairs skin barrier function in a human skin model. European Journal of Allergy and Clinical immunology (2013); 68(1): 37–47.
- Skin model: Neonatal keratinocytes were cultured in a microfluidic system to precisely examine the morphology, behavior and viability of individual cells. Adrian T. O’Neill, Nancy A. Monteiro-Riviere, and Glenn M. Walker. Characterization of microfluidic human epidermal keratinocyte culture. Cytotechnology (2008); 56(3): 197–207.
- Cancer: Keratinocytes were used in this study to demonstrate that dysregulated activation of type I interferon innate immunity is implicated in the molecular processes triggered by anti-EGFR drugs and leading to persistent skin inflammation. Carrie HS, Hong Y, Yunbo L, Jennifer SM, Sridevi B, Gaylene S, Judith AC and Thomas RS. EGF receptor signaling blocks aryl hydrocarbon receptor-mediated transcription and cell differentiation in human epidermal keratinocytes. Proceedings of National Academy of Sciences (2009); 106(11): 4266-71.
- Human Papillomaviruses (HPV) mediated skin cancer response: An infection of the skin with human papillomaviruses (HPV) may result in benign tumors with limited growth, which tend to regress spontaneously and causes non-melanoma skin cancer development in epidermodysplasia verruciformis (EV) and immunosuppressed patients. The present study utilized pooled keratinocytes cultured in KGMTM-Gold Medium from several healthy donors. The data showed that betaPV may increase the number of stem cell-like cells present during early carcinogenesis and thus enable the persistence and accumulation of DNA damage necessary to generate malignant stem cells. Martin H, Adrian B, Cinzia B, Marisa G, John D, Alan S, Herbert P, Ian M and Baki A. Expression of betapapillomavirus oncogenes increases the number of keratinocytes with stem cell-like properties. Journal of Virology (2013); 87(22): 12158-12165.
Content & Storage
Content
1 x Cryopreserved ampule of NHEK-Ad from single donor containing ≥ 500,000 cells
Data
Normal Human Epidermal Keratinocytes
Cryopreserved Normal Human Epidermal Keratinocytes are guaranteed through 18 population doublings for adult cells and 20 population doublings for neonatal cells
lonza,CC-3121,CC-2519,CC-2517,EBM™ 内皮细胞生长基础培养基500 mL
Catalog #: CC-3121
Basal media required for growth of Endothelial cells, 500 mL (supplements and growth factors sold separately)
Product Overview
EBMTM is an endothelial basal medium sold separately. This can be used with 1 kit of CC-4133 (endothelial cell growth medium SingleQuotsTM Supplements and Growth Factors for use with certain HUVEC cells).
Lonza guarantees the performance of CloneticsTM/PoieticsTM Cells only if appropriate CloneticsTM/PoieticsTM Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.
EBMTM 是一种内皮基础培养基，单独出售。 这可以与 1 套 CC-4133（内皮细胞生长培养基 SingleQuotsTM 补充剂和生长因子用于某些 HUVEC 细胞）一起使用。
只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。 如果您在选择合适的培养基、试剂或方案方面需要帮助，请联系 Lonza Scientific Support。
Content & Storage
Content
1 x Bottle EBMTM Basal Medium, 500 mL
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HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™ CC-2519
HUVEC – 人脐静脉内皮细胞，汇集，在 EGM™ CC-2519 中
EGM™ Endothelial Cell Growth Medium BulletKit™ CC-3124
EGM™ 内皮细胞生长培养基 BulletKit™ CC-3124
EGMTM Endothelial Cell Growth Medium SingleQuotsTM Kit CC-4133
EGMTM 内皮细胞生长培养基 SingleQuotsTM 试剂盒 CC-4133
lonza,PT-5006,PT-8202,hADSC 人类脂肪源性干细胞
Catalog #: PT-5006
Cryopreserved ampule of Human Adipose-Derived Stem Cells (hADSC) containing ≥1 million cells
Product Overview
PoieticsTM 正常人脂肪衍生干细胞 (hADSC) 是从在择期手术吸脂过程中收集的正常（非糖尿病）成人吸脂中分离出来的。
据报道，正常人 ADSC 可分化出许多不同的谱系，包括软骨形成、成骨、脂肪形成和神经。正常人 ADSC 在初次传代时已被冷冻保存。
相关的额外捐赠者信息，包括体重指数 (BMI) 可通过联系科学支持获得。冷冻保存的 PoieticsTM 正常人 ADSC 保证经过 5 次传代，表达 CD13、CD29、CD44、CD73、CD90、CD105 和 CD166，不表达 CD14、CD31 和 CD45。
所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。
来自糖尿病捐赠者的人类 ADSC 作为单独的产品提供。这些细胞表现出与骨髓来源的间充质干细胞非常相似的表型和功能特征。
Recommended Media and Reagents
Growth Medium:
- ADSC Human Adipose Derived Stem Cell Growth BulletKitTM Medium
Differentiation Medium*:
- Adipogenic: PGMTM -2 Preadipocyte Growth Medium-2 BulletKitTM Medium (provides both a plating medium and a differentiation medium appropriate to induce differentiation of human adipose derived mesenchymal stem cells into mature, functionally active adipocytes; Each bottle of the PGM-2TM BulletKitTM Medium contains enough differentiation medium to fully differentiate up to 20 million Adipose Derived Stem Cells (ADSC) [10 complete 96-well plates] into mature, functionally active adipocytes).
- Chondrogenic: hMSC Chondrogenic Differentiation BulletKitTM Medium, with the addition of TGF-β3 (provides a differentiation medium appropriate to induce chondrogenic differentiation of human adipose derived mesenchymal stem cells into mature, functionally active chondrocytes; Each bottle of the hMSC Chondrogenic Differentiation BulletKitTM Medium, with the addition of TGF-β3 (sold separately), contains enough differentiation medium to fully differentiate approximately 12.5 million human adipose derived mesenchymal stem cells [approximately 25 micropellets] into mature, functionally active chondrocytes).
- Osteogenic: hMSC Osteogenic Differentiation BulletKitTM Medium (provides a differentiation medium appropriate to induce osteogenic differentiation of human adipose derived mesenchymal stem cells into mature, functionally active osteoblasts; Each bottle of the hMSC Osteogenic Differentiation BulletKitTM Medium contains enough differentiation medium to fully differentiate approximately 200,000 human adipose derived mesenchymal stem cells [approximately 20 wells in a 24-well plate format] into mature, functionally active osteoblasts).
Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.
*Lonza does not routinely test differentiation of Lonza’s human adipose derived mesenchymal stem cells and cannot guarantee differentiation for all lots.
Benefits
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis provided for every cell lot
Content & Storage
Content
1 x Cryopreserved ampule hADSC containing ≥1 million cells
Instructions, SDS, CoA
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Human Mesenchymal Stem Cell (hMSC) Chondrogenic Differentiation Medium BulletKitTM PT-3003
Human Mesenchymal Stem Cell (hMSC) Osteogenic Differentiation Medium BulletKitTM PT-3002
人间充质干细胞 (hMSC) 成骨分化培养基 BulletKitTM PT-3002
ADSC Growth Medium BulletKit™ PT-4505
PGM-2TM Preadipocyte Growth Medium-2 BulletKitTM PT-8002
PGM-2TM 前脂肪细胞生长培养基-2 BulletKitTM PT-8002
MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ PT-3001
Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5007
Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5008
糖尿病 I 型和 II 型脂肪干细胞 (ADSC) PT-5008
hMSC – Human Mesenchymal Stem Cells PT-2501
HprAD – Human Subcutaneous Preadipocyte Cells PT-5001
Human Visceral Preadipocyte Cells PT-5005
DPSC – Human Dental Pulp Stem Cells PT-5025
AdipoRed™ Assay Reagent, 20 mL PT-7009
AdipoRed™ 检测试剂，20 mL PT-7009
OsteoImage™ Mineralization Assay, 500 Test PA-1503
OsteoImage™ 矿化测定，500 测试 PA-1503
lonza,CC-5035,CC-2935,EGM™-Plus 内皮细胞生长培养基-Plus BulletKit™
Catalog #: CC-5035
Culture system containing EBMTM Plus Basal Medium (CC-5036) and EGMTM Plus SingleQuotsTM supplements (CC-4542) required for growth of Endothelial cells. This media is optimized for use with Human Umbilical Vein Endothelial Cells (CC-2935).
包含内皮细胞生长所需的 EBMTM Plus 基础培养基 (CC-5036) 和 EGMTM Plus SingleQuotsTM 补充剂 (CC-4542) 的培养系统。 该培养基经过优化，可用于人脐静脉内皮细胞 (CC-2935)。
Product Overview
Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used to grow CloneticsTM Primary Cells.
从多种内皮细胞生长培养基中进行选择，以轻松培养原代内皮细胞。 每种内皮细胞生长培养基都有其特殊的配方，并针对某些细胞类型进行了优化。 每种类型的内皮细胞生长培养基在用于培养 CloneticsTM 原代细胞时都能保证发挥作用。
The following Endothelial Cell Growth Medium BulletKitsTM are available:
- EGMTM-2 BulletKitTM (CC-3162) – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Contains 2% FBS and VEGF for rapid proliferation. Does not contain BBE.
- EGMTM BulletKitTM (CC-3124) – Optimized for HUVECs cat. no. CC-2517 and CC-2519, contains 2% FBS and BBE. Does not contain exogenous VEGF.
- New! EGMTM-Plus BulletKitTM (CC-5035) – Enhanced version of EGMTM Medium. Optimized for HUVECs cat. no. CC-2935. Contains 2% FBS and BBE. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGMTM Medium.
- EGMTM-2 MV BulletKitTM (CC-3202) – Coronary and Iliac artery, human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. Does not contain BBE.
Lonza guarantees the performance of CloneticsTM/PoieticsTM Cells only if appropriate CloneticsTM/PoieticsTM Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.
只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
Content & Storage
Content
1 x EBMTM Plus Basal Medium (CC-5036), 475 mL
1 x EGMTM Plus SingleQuotsTM Supplement Pack (CC-4542) containing:
1 x Bottle FBS, 10.00 mL
1 x Vial Hydrocortisone, 0.50 mL
1 x Bottle L-Glutamine, 25.00 mL
1 x Vial EnGS, 1.00 mL
1 x Vial Ascorbic Acid, 0.50 mL
1 x Vial rhEGF, 0.50 mL
1 x Vial GA-1000, 0.50 mL
1 x Vial Heparin, 0.50 mL
Related Products
HUVEC – Human Umbilical Vein Endothelial Cells without VEGF, Single Donor, in EGM™-Plus CC-2935
HUVEC – EGM™-Plus CC-2935 中不含 VEGF 的人脐静脉内皮细胞，单一供体
EGM™-Plus Endothelial Cell Growth Medium – Plus SingleQuot™ Kits and Growth Supplements CC-4542
EGM™-Plus 内皮细胞生长培养基 – 加上 SingleQuot™ 试剂盒和生长补充剂 CC-4542
EBM™- Plus Endothelial Cell Growth Basal Medium-Plus CC-5036
EBM™- Plus 内皮细胞生长基础培养基-Plus CC-5036
lonza,CC-2535,CC-2904,CC-2903,CC-2585,V5SP-5002,HAEC-人主动脉内皮细胞
Catalog #: CC-2535
Cryopreserved ampule of Human Aortic Endothelial Cells (HAEC) containing ≥ 500,000 cells
含有 ≥ 500,000 个细胞的人主动脉内皮细胞 (HAEC) 冻存安瓿
Product Overview
Cardiac Endothelial Cells include cells isolated from the aorta (HAEC), coronary artery (HCAEC), pulmonary artery (HPAEC), iliac artery (HIAEC), and microvascular cardiac cells (HMVEC-C). Additional donor characteristics including hypertension, or smoking history may be available by contacting Scientific Support. Cardiac fibroblasts from matched donors are available for certain lots.
CloneticsTM Cells, Medium and Reagents are quality tested together and guaranteed to give optimum performance as a complete cell system. Cryopreserved HAECs are shipped in third passage.
Cryopreserved CloneticsTM Human Aortic Endothelial Cells (HAEC) are guaranteed through 15 population doublings, express CD31/105, von Williebrand Factor VIII, and are positive for acetyated low density lipoprotein uptake. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
心脏内皮细胞包括从主动脉 (HAEC)、冠状动脉 (HCAEC)、肺动脉 (HPAEC)、髂动脉 (HIAEC) 和微血管心脏细胞 (HMVEC-C) 分离的细胞。联系科学支持可以获取其他供体特征，包括高血压或吸烟史。来自匹配供体的心脏成纤维细胞可用于某些批次。
CloneticsTM 细胞、培养基和试剂一起经过质量测试，保证作为一个完整的细胞系统提供最佳性能。冷冻保存的 HAEC 在第三次运输中运输。
冷冻保存的 CloneticsTM 人主动脉内皮细胞 (HAEC) 通过 15 次群体倍增得到保证，表达 CD31/105、von Williebrand 因子 VIII，并且对乙酰化低密度脂蛋白摄取呈阳性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。
Benefits
- Guaranteed through 15 population doublings
- Test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- A Certificate of Analysis is provided for each cell lot purchased
Content & Storage
Content
1 x Cryopreserved ampule HAEC containing ≥ 500,000 cells
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NHCF-A – 人心房心脏成纤维细胞 CC-2903
NHCF-V – Human Ventricular Cardiac Fibroblasts CC-2904
NHCF-V – 人心室心脏成纤维细胞 CC-2904
AoAF – Human Aortic Adventitial Fibroblasts CC-7014
AoAF – 人主动脉外膜成纤维细胞 CC-7014
HCAEC – Human Coronary Artery Endothelial Cells CC-2585
HCAEC – 人冠状动脉内皮细胞 CC-2585
EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™ CC-3162
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P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5012
P5 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-5012
P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5024
P5 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-5024
P5 Primary Cell 4D-Nucleofector™ X Kit S V4XP-5032
P5 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-5032
P5 Primary Cell 96-well Nucleofector™ Kit V4SP-5096
P5 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-5096
P5 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-5960
P5 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-5960
P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5002
P5 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-5002
P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5010
P5 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-5010
Data
HAEC – Human Aortic Endothelial Cells
Human aortic endothelial cells (CC-2535) were immuno histochemically stained with von Willebrand Factor antibody (Sigma, F3520) used at 1:200. Secondary antibody used was VECTDI1094 DyLight 594 (Vector Laboratories). Cells were also satined with CD31/PECAM-1 antibody (R&D Systems, BBA7) used at 1:100 (green). Secondary antibody used to detect was VECTDI2488 DyLight 488 (Vector Laboratories) Hoechst nuclear stain (blue).
用 1:200 使用的血管性血友病因子抗体 (Sigma, F3520) 对人主动脉内皮细胞 (CC-2535) 进行免疫组织化学染色。 使用的二抗是 VECTDI1094 DyLight 594 (Vector Laboratories)。 细胞也用 1:100（绿色）使用的 CD31/PECAM-1 抗体（R&D Systems，BBA7）饱和。 用于检测的二抗是 VECTDI2488 DyLight 488（Vector Laboratories）Hoechst 核染色剂（蓝色）。
HAEC – Human Aortic Endothelial Cells
Human aortic endothelial cells were immunohistochemically stained with VE Cadherin antibody (Santa Cruz,sc-28644) used at 1:200 dilution (red). Secondary antibody used was VECTDI1094 DyLight 594 (Vector Laboratories). Nuclei were stained with Hoechst stain at 1:10,000 dilution.
用 1:200 稀释度（红色）的 VE 钙粘蛋白抗体（Santa Cruz，sc-28644）对人主动脉内皮细胞进行免疫组织化学染色。 使用的二抗是 VECTDI1094 DyLight 594 (Vector Laboratories)。 细胞核以 1:10,000 稀释度用 Hoechst 染料染色。
lonza,CC-5002,CC-5034,胰蛋白酶中和溶液100 mL
Catalog #: CC-5002
Trypsin Neutralizing Solution for use in cell culture, 100 mL
Product Overview
Trypsin Neutralizing Solution (TNS) is used to effectively neutralize Trypsin/EDTA. Lonza’s TNS is available individually or as a component in our ReagentPackTM Subculture Reagents (CC-5034) which includes Trypsin and HEPES buffer.
胰蛋白酶中和溶液 (TNS) 用于有效中和胰蛋白酶/EDTA。 Lonza 的 TNS 可以单独使用，也可以作为我们的 ReagentPackTM Subculture Reagents (CC-5034) 的一个组件使用，其中包括胰蛋白酶和 HEPES 缓冲液。
Content & Storage
Content
1 x Bottle Trypsin Neutralizing Solution, 100 mL
Instructions, SDS, CoA
Related Products
ReagentPack™ Subculture Reagents, 100 mL CC-5034
ReagentPack™ 继代培养试剂，100 mL CC-5034
lonza,CC-2571,V5SP-1002,CC-2914,AoSMC-人主动脉平滑肌细胞
Catalog #: CC-2571
Cryopreserved ampule of Human Aortic Smooth Muscle Cells (AoSMC) containing ≥ 500,000 cells
含有 ≥ 500,000 个细胞的人主动脉平滑肌细胞 (AoSMC) 冻存安瓿
Product Overview
Human Vascular Smooth Muscle Cells come from multiple tissue types including Aorta (AoSMC), Coronary Artery (CASMC), Pulmonary Artery (PASMC), and Umbilical Artery (UASMC). Cryopreserved cells are shipped in 3rd passage.
CloneticsTM Human Aortic Smooth Muscle Cells are guaranteed for 15 population doublings and to stain positive for alpha smooth muscle actin and negative for von Willebrand Factor VIII after differentiation. All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.
人血管平滑肌细胞来自多种组织类型，包括主动脉 (AoSMC)、冠状动脉 (CASMC)、肺动脉 (PASMC) 和脐动脉 (UASMC)。 冷冻保存的细胞在第 3 代运输。
CloneticsTM 人主动脉平滑肌细胞保证 15 次群体倍增，分化后 α 平滑肌肌动蛋白染色呈阳性，von Willebrand 因子 VIII 染色呈阴性。 所有细胞的支原体、细菌、酵母菌和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书 (CoA)。
Applications
- Stroke
- Diabetes
- Vascular research
- Artherosclerosis
- Hypertension
Content & Storage
Content
1 x Cryopreserved ampule of AoSMC containing ≥ 500,000 cells/vial
Related Products
HAEC – Human Aortic Endothelial Cells CC-2535
AoAF – Human Aortic Adventitial Fibroblasts CC-7014
NHCF-A – Human Atrial Cardiac Fibroblasts CC-2903
NHCF-V – Human Ventricular Cardiac Fibroblasts CC-2904
NHCF-V – 人心室心脏成纤维细胞 CC-2904
D-AoSMC – Diseased Human Aortic Smooth Muscle Cells – Diabetes Type I CC-2914
D-AoSMC – 患病的人主动脉平滑肌细胞 – I 型糖尿病 CC-2914
D-AoSMC – Diseased Human Aortic Smooth Muscle Cells – Diabetes Type II CC-2916
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1012
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1024
P1 Primary Cell 4D-Nucleofector™ X Kit S V4XP-1032
P1 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-1032
P1 Primary Cell 96-well Nucleofector™ Kit V4SP-1096
P1 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-1096
P1 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-1960
P1 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-1960
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1002
P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1002
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1010
P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1010
AoSMC – Human Aortic Smooth Muscle Cells
Human aortic smooth muscle cells (CC-2571) were grown in flasks and subsequently sub-cultured into 24-well plates, at 20k cells in Smooth muscle cell growth medium SmGM-2 medium (CC-3182). Cells were fixed and immunostained with 1:1500 dilution of anti-smooth muscle actin antibody (CBL171, Sigma), and nuclei were stained with Hoechst stain.
人主动脉平滑肌细胞 (CC-2571) 在烧瓶中生长，随后在平滑肌细胞生长培养基 SmGM-2 培养基 (CC-3182) 中以 20k 个细胞传代培养到 24 孔板中。 将细胞固定并用 1:1500 稀释的抗平滑肌肌动蛋白抗体 (CBL171, Sigma) 进行免疫染色，细胞核用 Hoechst 染料染色。
AoSMC – Human Aortic Smooth Muscle Cells
Phase contrast images of human aortic smooth muscle cells (CC-2571) cultured in SmGM-2 medium (CC-3182).
在 SmGM-2 培养基 (CC-3182) 中培养的人主动脉平滑肌细胞 (CC-2571) 的相差图像。
lonza,CC-4175,CC-2540,CC-3170,BEGMTM支气管上皮SingleQuotsTM试剂盒
Catalog #: CC-4175
包含培养支气管上皮细胞所需的生长因子和补充剂（不包括基础培养基）
Product Overview
BEGMTM SingleQuotTM Kits are components sold separately and does not include the basal medium. These are part of the BEGMTM Bronchial Epithelial Cell Growth Medium BulletKitTM (catalog # CC-3170) which consists of basal medium and supplements packaged together.
BEGMTM Bronchial Epithelial Cell Growth Medium is specially designed to support the growth of human primary bronchial epithelial cells. BEGMTM BulletKitTM is serum-free and has been optimized to grow cells from normal and diseased tissues.
BEGMTM is an optimized and readily available media kit providing researchers the confidence and the convenience of a system that works to support their cells. This is especially important for researchers who are utilizing cell lines as a stepping stone to primary cell culture. Working with the same BEGMTM Media between cell lines and primary NHBE can reduce variability within the experiments and simplify the shift for researchers. In addition, if primary cells are utilized as a side-by-side control with normal cell lines or home-grown bronchial epithelial cells, the BEGMTMBulletKitTM Media may also offer cost-benefit since customers can use the same media to support multiple cell types.
BEGMTM SingleQuotTM 试剂盒是单独出售的组件，不包括基础培养基。这些是 BEGMTM 支气管上皮细胞生长培养基 BulletKitTM（目录号 CC-3170）的一部分，它由包装在一起的基础培养基和补充剂组成。
BEGMTM 支气管上皮细胞生长培养基专为支持人原代支气管上皮细胞的生长而设计。 BEGMTM BulletKitTM 不含血清，经过优化，可从正常和患病组织中培养细胞。
BEGMTM 是一种经过优化且随时可用的培养基套件，可为研究人员提供支持其细胞的系统的信心和便利性。这对于利用细胞系作为原代细胞培养垫脚石的研究人员来说尤其重要。在细胞系和原代 NHBE 之间使用相同的 BEGMTM 培养基可以减少实验中的变异性并简化研究人员的转变。此外，如果将原代细胞用作与正常细胞系或本土支气管上皮细胞并排的对照，BEGMTMBulletKitTM 培养基还可以提供成本效益，因为客户可以使用相同的培养基来支持多种细胞类型。
Here are publications out of many where our customers have used BEGMTM Growth Media with normal bronchial epithelial cells:
- El Najjar, et al. (2015). PLoSONE 10(2) demonstrates culturing of BEAS-2B bronchial epithelial cell line in BEGMTM Media to examine the effects of cathepsin and furin proteolytic enzymes on viral fusion protein activation in cells
- Hagiwara, et al. (2015). Nature demonstrates BEGMTM Media can ideally support co-culture of Lonza’s HUVEC and NHBE in a 3D environment.
- Rider, et al. (2015). PLoS ONE 10(1) used BEGMTM Media to culture primary human bronchial epithelial cells obtained from non-transplanted normal lungs
Purchase our Bronchial Epithelial Cell Culture Kit (catalog #. CC-2540B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of bronchial epithelial cells (catalog # CC-2540) and BEGMTM BulletKitTM Growth Media (catalog#. CC-3170).
购买我们的支气管上皮细胞培养试剂盒（目录号 CC-2540B）并获得细胞和培养基的折扣价。 该试剂盒由冷冻保存的支气管上皮细胞安瓿（目录号 CC-2540）和 BEGMTM BulletKitTM 生长培养基（目录号 CC-3170）组成。
Content & Storage
Content
1 x Orange Cap Vial with BPE 2.00 mL
1 x Lilac Cap Vial with Insulin 0.50 mL
1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Amber Vial with Retinoic Acid 0.50 mL
1 x Natural Cap Vial with Transferrin 0.50 mL
1 x Amber Vial with Triiodothyronine 0.50 mL
1 x Amber Vial with Epinephrine 0.50 mL,
1 x Green Cap Vial with hEGF 0.50 mL
Instructions, SDS, CoA
Related Products
BEGM™ Bronchial Epithelial Cell Growth Medium BulletKit™ CC-3170
BEGM™ 支气管上皮细胞生长培养基 BulletKit™ CC-3170
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540
NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞
NHBE – Human Bronchial/Tracheal Epithelial Cells without Retinoic Acid CC-2541
NHBE – 不含维甲酸 CC-2541 的人支气管/气管上皮细胞
D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma 00194911
D-HBE-As – 患病的人支气管/气管上皮细胞 – 哮喘 00194911
D-HBE-COPD – Diseased Human Bronchial/Tracheal Epithelial Cells – COPD 00195275
D-HBE-COPD – 患病的人支气管/气管上皮细胞 – COPD 00195275
lonza,lonza CC-2519,V4SP-3960,HUVEC – 人脐静脉内皮细胞EGM™
Catalog #: CC-2519
Cryopreserved ampule of Human Umbilical Vein Endothelial Cells (HUVEC) from pooled donors, containing ≥ 500,000 cells
Product Overview
Lonza’s Cryopreserved HUVECs are shipped as frozen primaries. They are guaranteed to express CD31/105 and survive in culture through 15* population doublings (*except HUVEC-XLTM guaranteed through 5 PD).
All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Our pooled vs. single donor options allow researchers to study or exclude donor variability from their experiments. Depending upon the study, pooled versions are typically utilized to reduce donor variability while single donor options allow researchers to assess donor-specific differences.
HUVEC-XL size consists of 10 million cells/amp achieved by pooling donors. This option is designed to support studies that require large volume of cells while still offering cost-benefits to researchers.
Lonza 的冷冻保存的 HUVEC 作为冷冻初级产品运输。 它们保证表达 CD31/105 并通过 15* 种群倍增在培养中存活（* HUVEC-XLTM 除外，保证通过 5 PD）。
所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。
我们的汇集与单一供体选项允许研究人员在他们的实验中研究或排除供体变异性。 根据研究，汇总版本通常用于减少捐赠者的可变性，而单一捐赠者选项允许研究人员评估特定捐赠者的差异。
HUVEC-XL 大小由 1000 万个细胞/安培组成，通过汇集供体实现。 此选项旨在支持需要大量细胞的研究，同时仍为研究人员提供成本效益。
Not All HUVECs Are Created Equal – Which Type Is Suitable for Your Research?
- CC-2517 (single donor), CC-2519 (pooled donor) – Both isolated in EGMTM Media, in absence of defined growth factors. Contains BBE. No exogenous VEGF.
- CC-2935 (single donor) – Isolated in EGMTM – Plus Media: Improved version of part number CC-2517. Offers faster proliferation rates than HUVEC isolated in EGMTM Media. No exogenous VEGF.
- C2517A (single donor), C2519A (pooled donor), 00191027 (XL size) – Isolated in EGMTM-2 Media, in presence of growth factors such as VEGF, rhFGF, rhEGF, and others. Does not contain BBE.
- C2517AS (single donor), C2519AS (pooled donor) – Pre-screened HUVECs in EGMTM-2 Media. Screened for angiogenesis markers and test positive for expression of eNOS, Axl, Tie-2 and VEGFr2.
Benefits
- Guaranteed through 15 population doublings
- Single or pooled donors available
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis provided for every cell lot
Applications
- Would healing
- Angiogenesis
- Tissue engineering
- Inflammation
- Oncology
- Pharmacology
- Vascular modeling
- Transfection
Content & Storage
Content
1 x Cryopreserved ampule HUVEC (pooled donor) containing ≥500,000 cells
Instructions, SDS, CoA
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RAFT™ 24-Well Small Kit, 3D Reagents & 24-Well Plate Absorbers – 12 Reactions 016-1R16
HAEC – Human Aortic Endothelial Cells CC-2535
HCAEC – Human Coronary Artery Endothelial Cells CC-2585
EGM™ Endothelial Cell Growth Medium BulletKit™ CC-3124
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032
P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010
HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2, Prescreened for Angiogenesis C2517AS
HUVEC – 人脐静脉内皮细胞，单一供体，在 EGM™-2 中，预先筛选血管生成 C2517AS
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™-2 C2519A
HUVEC – 人脐静脉内皮细胞，混合，在 EGM™-2 C2519A 中
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™-2, Prescreened for Angiogenesis C2519AS
HUVEC – 人脐静脉内皮细胞，汇集在 EGM™-2 中，预先筛选血管生成 C2519AS
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™ CC-2519
HUVEC – 人脐静脉内皮细胞，汇集，在 EGM™ CC-2519 中
HUVEC – Human Umbilical Vein Endothelial Cells without VEGF, Single Donor, in EGM™-Plus CC-2935
HUVEC – EGM™-Plus CC-2935 中不含 VEGF 的人脐静脉内皮细胞，单一供体
lonza,lonza CC-3211,LGM-3TM 淋巴细胞生长培养基-3
Catalog #: CC-3211
Serum-free growth medium required for growth of human lymphocytes and dendritic cells, 500 mL
Overview
Product Overview
LGM-3TM 淋巴细胞生长培养基是一种化学成分明确、无血清、无异种物质的培养基，开发用于支持（添加适当的细胞因子）人淋巴细胞和树突状细胞的生长和支持。 LGM-3TM 淋巴细胞生长培养基不含任何外源性生长因子、细胞增殖的人工刺激剂或未定义的补充剂。
LGM-3TM 淋巴细胞生长培养基不含任何蛋白激酶 C 刺激物，适用于研究第二信使系统在激活人类淋巴细胞中的作用。 LGM-3TM 淋巴细胞生长培养基的配方是完整的，包含人白蛋白、重组人胰岛素和巴氏杀菌人转铁蛋白。 LGM-3TM 淋巴细胞生长培养基可根据应用进一步补充额外的细胞因子和生长因子。
只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
Content & Storage
Content
1 x LGM-3TM Lymphocyte Growth Medium-3, 500 mL
Instructions, SDS, CoA
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NK – Human Natural Killer Cells 2W-502
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Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
lonza,lonza PT-3002,人间充质干细胞 (hMSC) 成骨分化培养基 BulletKitTM
Catalog #: PT-3002
Culture system containing required basal medium and supplements for hMSC osteogenic differentiation
Product Overview
Stem cells possess the unique ability to differentiate into multiple lineages of cells including osteoblasts. Lonza’s hMSC Osteogenic Differentiation BulletKitTM Medium is a serum containing medium designed to induce osteogenic differentiation of human bone marrow derived mesenchymal stem cells (hMSC) into mature, functionally active osteoblasts in as few as 14 days. Differentiation can then be validated by a variety of methods including the use of Lonza’s OsteoImageTM Bone Mineralization Reagent which provides a quantitative value related to the amount of osteogenic differentiation.
干细胞具有分化成包括成骨细胞在内的多种细胞谱系的独特能力。 Lonza 的 hMSC 成骨分化 BulletKitTM 培养基是一种含有血清的培养基，旨在在短短 14 天内诱导人骨髓间充质干细胞 (hMSC) 成骨分化成成熟、功能活跃的成骨细胞。 然后可以通过多种方法验证分化，包括使用 Lonza 的 OsteoImageTM 骨矿化试剂，该试剂提供与成骨分化量相关的定量值。
Partial List of Cells Differentiated with hMSC Osteogenic Differentiation BulletKitTM Medium*:
- Human bone marrow derived stem cells
- Human umbilical cord blood derived stem cells
- Human umbilical cord matrix derived stem cells
- C57BL/6 mice bone marrow derived stem cells
- Human adipose derived stem cells
- Human periodontal ligament derived stem cells
- Human hamstring tendon derived stem cells
The hMSC Osteogenic Differentiation BulletKitTM Medium provides a differentiation medium guaranteed to induce osteogenic differentiation of bone marrow derived hMSCs into mature, functionally active osteoblasts; Each bottle of the hMSC Osteogenic Differentiation BulletKitTM Medium contains enough differentiation medium to fully differentiate up to 750,000 bone marrow derived hMSCs [approximately 25 wells in a 6-well plate format] into mature, functionally active osteoblasts.
The hMSC Osteogenic Differentiation Medium is offered as a BulletKitTM Medium (catalog no. PT-3002) which includes both the basal media and the necessary supplements for osteogenic differentiation of bone marrow derived hMSCs.
Lonza guarantees the performance of cells only if appropriate media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.
*List of cells generated from customer data. Lonza only routinely uses this medium with Lonza’s human bone marrow derived mesenchymal stem cells and can therefore only guarantee the successful use of this media with these cells.
hMSC 成骨分化 BulletKitTM 培养基提供了一种分化培养基，可保证诱导骨髓来源的 hMSC 成骨分化为成熟的、功能活跃的成骨细胞；每瓶 hMSC 成骨分化 BulletKitTM 培养基都含有足够的分化培养基，可以将多达 750,000 个骨髓来源的 hMSCs [大约 25 个孔，以 6 孔板形式] 完全分化成成熟的、功能活跃的成骨细胞。
hMSC 成骨分化培养基以 BulletKitTM 培养基（目录号 PT-3002）的形式提供，其中包括基础培养基和骨髓衍生 hMSC 成骨分化所需的补充剂。
只有在专门使用适当的培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
*从客户数据生成的单元格列表。 Lonza 仅常规地将这种培养基与 Lonza 的人骨髓来源的间充质干细胞一起使用，因此只能保证这种培养基与这些细胞一起成功使用。
Content & Storage
Content
1 x hMSC Osteogenic Differentiation Basal Medium (PT-3924), 170 mL
1 x hMSC Osteogenic Differentiation SingleQuotsTM Supplements Kit (PT-4120)
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lonza,原代细胞和干细胞,CC-3107,KGM™-2 角质形成细胞生长培养基-2 BulletKit™
Catalog #: CC-3107
Culture system containing KBMTM-2 Basal Medium (CC-3103) and KGMTM-2 SingleQuotsTM Supplements (CC-4152) required for growth of Keratinocytes
Product Overview
KGMTM-2 角质形成细胞生长培养基 BulletkitTM 也作为单独的基础培养基和补充剂出售。
角质形成细胞生长培养基 2 已针对无血清环境中的角质形成细胞增殖进行了优化，通常提供比 KGMTM 培养基更高的生长速率。
CloneticsTM 培养基产品专为支持人和动物原代细胞的生长而设计。 媒体系统已针对各种细胞系统专门开发和优化。
Content & Storage
Content
1 x KBMTM-2 Basal Medium (CC-3103), 500 mL
1 x KGMTM-2 SingleQuotsTM Supplement Pack (CC-4152) containing:
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Natural Cap Vial with Transferrin, 0.50 mL
1 x Amber Vial with Epinephrine, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
Instructions, SDS, CoA
Safety Data Sheets (SDS)
View SDS
SDS – German
SDS – Italian
SDS – Dutch
SDS – French
SDS – Spanish
SDS – English
lonza,CC-2553,RPTEC – 人肾近端小管上皮细胞
Catalog #: CC-2553
Cryopreserved ampule of Human Renal Proximal Tubule Epithelial Cells (RPTEC) containing ≥ 500,000 cells
Product Overview
Lonza’s Human Renal Cells include cells isolated from various normal human kidney tissue specific to each cell type. Human Renal Proximal Tubule Cells are isolated from the proximal tubule and are ideal for drug transporter studies. Human Renal Epithelial Cells (HRE) are comprised of cells from the cortex and glomerular region. Human Renal Cortical Cells (HRCE) are from proximal and distal tubules. Human Mesangial Cells (HMC) are isolated from the renal glomerulus and modified smooth muscle cells between capillaries.
Renal epithelial cells display a polarized morphology, which is essential for their function. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate, while allowing other substances of no nutritional value to be excreted in the urine. Physiological processes like osmoregulation and excretion can be studied with HRE in vitro. Human renal epithelial cells are also capable of internalizing bacteria and are located near injury sites in a variety of congenital, metabolic and inflammatory diseases.
Lonza 的人肾细胞包括从对每种细胞类型特异的各种正常人肾组织中分离出来的细胞。人肾近端小管细胞从近端小管中分离出来，是药物转运蛋白研究的理想选择。人肾上皮细胞 (HRE) 由来自皮质和肾小球区域的细胞组成。人肾皮质细胞 (HRCE) 来自近端和远端小管。人系膜细胞 (HMC) 从肾小球和毛细血管之间的修饰平滑肌细胞中分离出来。
肾上皮细胞表现出极化形态，这对其功能至关重要。它们重新吸收肾小球滤液中几乎所有的葡萄糖和氨基酸，同时允许其他没有营养价值的物质从尿液中排出。渗透调节和排泄等生理过程可以在体外用 HRE 进行研究。人肾上皮细胞也能够内化细菌，并且位于各种先天性、代谢和炎症性疾病的损伤部位附近。
Select References with Lonza’s Renal Cells and Media
- Renal Physiology: Intercellular communication is a key process in the development and maintenance of multicellular organisms. Study mentioned here reported extensive spontaneous intercellular exchange of cargo vesicles and organelles between primary human proximal tubular epithelial cells. Sophie Domhan, Lili Ma, Albert Tai, Zachary Anaya, Afshin Beheshti, Martin Zeier, Lynn Hlatky, Amir Abdollahi. Intercellular Communication by Exchange of Cytoplasmic Material via Tunneling Nano-Tube Like Structures in Primary Human Renal Epithelial Cells. PLoS ONE (2011); 6(6): e21283
- Nephrotoxicity study was done to evaluate the direct toxicity of Streptozotocin on human renal proximal tubule epithelial cells which resulted in upregulation of the activated form of the endoplasmic reticulum stress (ER stress) in the renal epithelial cells. Uetake R, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Iesato Y, Yoshizawa T, Koyama T, Yang L, Toriyama Y, Yamauchi A, Igarashi K, Tanaka M, Kuwabara T, Mori K, Yanagita M, Mukoyama M, Shindo T. Adrenomedullin-RAMP2 system suppresses ER stress-induced tubule cell death and is involved in kidney protection. PLoS ONE (2014); 9(2): e87667
- Renal Drug Transporters: Study was done using Lonza’s human renal proximal tubule cells (CC-2553), where the effect of Cobicistat (COBI) on the cytotoxicity of Tenofovir (TFV) in renal cell cultures was examined to elucidate renal drug-drug interaction between these two components. Kirsten M. Stray, Rujuta A. Bam, Gabriel Birkus, Jia Hao, Eve-Irene Lepist, Stephen R. Yant, Adrian S. Ray, Tomas Cihlar. Evaluation of the effect of cobicistat on the in vitro renal transport and cytotoxicity potential of tenofovir. Antimicrobial Agents and Chemotherapy (2013); 57(10): 4982-4989
Benefits
- Guaranteed through 15 population doublings
- Cells stain positive for gamma GTP and pan cytokeratin
- Test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- A Certificate ofAnalysis is provided for each cell lot purchased
Content & Storage
Content
1 x Cryopreserved ampule of RPTEC containing ≥500,000 cells
Instructions, SDS, CoA
Related Products
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540
InEpC – Human Intestinal Epithelial Cells CC-2931
H-RPE – Human Retinal Pigment Epithelial Cells 00194987
REGM™ Renal Epithelial Cell Growth Medium BulletKit™ CC-3190
REGM™ 肾上皮细胞生长培养基 BulletKit™ CC-3190
lonza, CC-2540S,用于 B-ALITM 培养的 NHBE 人支气管/气管上皮细胞
Catalog #: CC-2540S
Cryopreserved Normal Human Bronchial Epithelial (NHBE) cells, screened and guaranteed for differentiation, in B-ALITM (Bronchial Air Liquid Interface) cell culture.
Product Overview
正常和患病的支气管上皮（NHBE 和 DHBE）细胞是从肺分叉上方的气道上皮衬里分离出来的。 CC-2540S 是适用于气液界面细胞培养的 NHBE，可保证以纤毛形成、粘蛋白产生和 TEER 水平为标志的分化。
所有冷冻保存的人支气管上皮细胞通过 15 次群体倍增得到保证。 细胞对支原体、细菌、酵母和真菌检测呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。
Select Applications with Lonza’s Human Bronchial Epithelial Cells
- A Rotating Bioreactor for Scalable Culture and Differentiation of Respiratory Epithelium”. Respiratory epithelium is difficult to grow as it requires a well-maintained polarizing air‐liquid interface (ALI) to maintain differentiation. Raredon et al. describe a scalable and easy method to conduct air-liquid interface with Lonza’s bronchial epithelial cells guaranteed for ALI differentiation (Cat. No. CC-2540S).
Content & Storage
Content
1 x Cryopreserved ampule containing ≥500,000 cells
Instructions, SDS, CoA
Related Products
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540
NHBE – Human Bronchial/Tracheal Epithelial Cells without Retinoic Acid CC-2541
SAEC – Human Small Airway Epithelial Cells CC-2547
HMVEC-L – Human Lung Microvascular Endothelial Cells CC-2527
D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma 00194911
D-HBE-COPD – Diseased Human Bronchial/Tracheal Epithelial Cells – COPD 00195275
D-HBE-CF – Diseased Human Bronchial/Tracheal Epithelial Cells – Cystic Fibrosis 00196979
BEGM™ Bronchial Epithelial Cell Growth Medium BulletKit™ CC-3170
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
lonza,CC-2509,NHDF-Neo-人类皮肤成纤维细胞，新生儿
Catalog #: CC-2509
Cryopreserved ampule of Normal Human Neonatal Dermal Fibroblasts (NHDF-Neo) containing ≥ 500,000 cells
Product Overview
人类皮肤成纤维细胞可从成人皮肤 (NHDF-Ad) 或新生儿包皮 (NHDF-Neo) 中获得。 它们来源于正常人类新生儿包皮或成人皮肤的真皮。 它们在原代培养结束时被冷冻保存。
使用 FGMTM-2 生长培养基时，Lonza 的人类真皮成纤维细胞可通过 15 倍的种群倍增得到保证。 所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 真皮成纤维细胞的特征在于贯穿连续传代的形态观察。 为购买的每个细胞批次提供分析证书。
Benefits
Applications:
- ECM protein analysis
- Wound healing
- Collagen metabolism
- Cosmetics
- Skin therapy/models
Content & Storage
Content
1 x Cryopreserved ampule of NHDF-Neo containing ≥ 500,000 cells
Related Products
NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled 00192906
NHEM-Neo – Human Epidermal Melanocytes, Neonatal CC-2504
NHEK-Neo – Human Epidermal Keratinocytes – Neonatal, Single Donor 00192907
HMVEC-dNeo – Human Dermal Microvascular Endothelial Cells, Neonatal, Single Donor CC-2505
HMVEC-dNeo – Human Dermal Microvascular Endothelial Cells, Neonatal, Pooled CC-2516
NHDF-Ad – Human Dermal Fibroblasts, Adult CC-2511
FGM™-2 Fibroblast Growth Medium-2 BulletKit™ CC-3132
P2 Primary Cell 4D-Nucleofector™ X Kit L V4XP-2012
P2 Primary Cell 4D-Nucleofector™ X Kit L V4XP-2024
P2 Primary Cell 4D-Nucleofector™ X Kit S V4XP-2032
P2 Primary Cell 96-well Nucleofector™ Kit V4SP-2096
P2 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-2096
P2 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-2960
P2 Primary Cell 384-well Nucleofector™ Kit V5SP-2002
P2 Primary Cell 384-well Nucleofector™ Kit V5SP-2010
P2 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-2010
lonza,lonza CC-2517,HUVEC – EGM™ 中的人脐静脉内皮细胞，单一供体
Catalog #: CC-2517
Cryopreserved ampule of Human Human Umbilical Vein Endothelial Cells (HUVEC) from a single donor containing ≥ 500,000 cells
来自单个供体的人脐静脉内皮细胞 (HUVEC) 冻存安瓿，含有 ≥ 500,000 个细胞
Product Overview
Lonza’s Cryopreserved HUVECs are shipped as frozen primaries. They are guaranteed to express CD31/105 and survive in culture through 15* population doublings (*except HUVEC-XLTM guaranteed through 5 PD).
All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Our pooled vs. single donor options allow researchers to study or exclude donor variability from their experiments. Depending upon the study, pooled versions are typically utilized to reduce donor variability while single donor options allow researchers to assess donor-specific differences.
HUVEC-XL size consists of 10 million cells/amp achieved by pooling donors. This option is designed to support studies that require large volume of cells while still offering cost-benefits to researchers.
Lonza 的冷冻保存的 HUVEC 作为冷冻初级产品运输。 它们保证表达 CD31/105 并通过 15* 种群倍增在培养中存活（* HUVEC-XLTM 除外，保证通过 5 PD）。
所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。
我们的汇集与单一供体选项允许研究人员在他们的实验中研究或排除供体变异性。 根据研究，汇总版本通常用于减少捐赠者的可变性，而单一捐赠者选项允许研究人员评估特定捐赠者的差异。
HUVEC-XL 大小由 1000 万个细胞/安培组成，通过汇集供体实现。 此选项旨在支持需要大量细胞的研究，同时仍为研究人员提供成本效益。
Not All HUVECs Are Created Equal – Which Type Is Suitable for Your Research?
- CC-2517 (single donor), CC-2519 (pooled donor) – Both isolated in EGMTM Media, in absence of defined growth factors. Contains BBE. No exogenous VEGF.
- CC-2935 (single donor) – Isolated in EGMTM – Plus Media: Improved version of part number CC-2517. Offers faster proliferation rates than HUVEC isolated in EGMTM Media. No exogenous VEGF.
- C2517A (single donor), C2519A (pooled donor), 00191027 (XL size) – Isolated in EGMTM-2 Media, in presence of growth factors such as VEGF, rhFGF, rhEGF, and others. Does not contain BBE.
- C2517AS (single donor), C2519AS (pooled donor) – Pre-screened HUVECs in EGMTM-2 Media. Screened for angiogenesis markers and test positive for expression of eNOS, Axl, Tie-2 and VEGFr2.
Benefits
- Guaranteed through 15 population doublings
- Pooled or single donors available
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis provided for every cell lot
Applications
- Would healing
- Angiogenesis
- Tissue engineering
- Inflammation
- Oncology
- Pharmacology
- Vascular modeling
- Transfection
Content & Storage
Content
1 x Cryopreserved ampule of HUVEC containing ≥ 500,000 cells
Instructions, SDS, CoA
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RAFT™ 24-Well Small Kit, 3D Reagents & 24-Well Plate Absorbers – 12 Reactions 016-1R16
HAEC – Human Aortic Endothelial Cells CC-2535
HCAEC – Human Coronary Artery Endothelial Cells CC-2585
HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2, Prescreened for Angiogenesis C2517AS
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™-2 C2519A
HUVEC – 人脐静脉内皮细胞，混合，在 EGM™-2 C2519A 中
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™-2, Prescreened for Angiogenesis C2519AS
HUVEC – 人脐静脉内皮细胞，汇集在 EGM™-2 中，预先筛选血管生成 C2519AS
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™ CC-2519
HUVEC – 人脐静脉内皮细胞，汇集，在 EGM™ CC-2519 中
HUVEC – Human Umbilical Vein Endothelial Cells without VEGF, Single Donor, in EGM™-Plus CC-2935
HUVEC – EGM™-Plus CC-2935 中不含 VEGF 的人脐静脉内皮细胞，单一供体
lonza,CC-2585,HCAEC-人冠状动脉内皮细胞
Catalog #: CC-2585
Cryopreserved ampule of Human Coronary Artery Endothelial Cells (HCAEC) containing ≥ 500,000 cells
Product Overview
心脏内皮细胞包括从主动脉 (HAEC)、冠状动脉 (HCAEC)、肺动脉 (HPAEC)、髂动脉 (HIAEC) 和微血管心脏细胞 (HMVEC-C) 分离的细胞。 联系科学支持可以获取其他供体特征，包括高血压或吸烟史。
CloneticsTM 细胞、培养基和试剂一起经过质量测试，保证作为一个完整的细胞系统提供最佳性能。 冷冻保存的 HCAEC 在第三通道中运输。
冷冻保存的 CloneticsTM 人冠状动脉内皮细胞 (HCAEC) 保证通过 15 次群体倍增，表达 CD31/105、von Williebrand 因子 VIII，并且对乙酰化低密度脂蛋白摄取呈阳性。 所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。
Benefits
- Assured for experimental use up to 15 population doublings
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis provided for every cell lot
Applications
- Atherosclerosis
- Angiogenesis
- Cell-to-cell Junctions
- Inflammation
- Wound Healing
- Oncology
Content & Storage
Content
1 x Cryopreserved ampule of HCAEC containing ≥ 500,000 cells/vial
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D-CASMC – Diseased Human Coronary Artery Smooth Muscle Cells – Diabetes Type II CC-2918
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EGMTM -2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM CC-3202
EGMTM -2 MV 微血管内皮细胞生长培养基-2 BulletKitTM CC-3202
P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5012
P5 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-5012
P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5024
P5 Primary Cell 4D-Nucleofector™ X Kit S V4XP-5032
P5 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-5032
P5 Primary Cell 96-well Nucleofector™ Kit V4SP-5096
P5 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-5960
P5 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-5960
P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5002
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lonza,CC-2543,HMVEC-dAd -人真皮微血管内皮细胞 -成人
Catalog #: CC-2543
Cryopreserved ampule of Adult Human Dermal Microvascular Endothelial Cells (HMVEC-d Ad) containing ≥ 500,000 cells
Product Overview
人真皮微血管内皮细胞包括从成人或新生儿包皮的皮肤组织内的小血管中分离出来的细胞。培养的细胞纯度≥90%，并专门富集真皮 LEC 和 BEC。来自新生儿或成人的真皮微血管内皮细胞 (HMVEC-D) 是血液 (HMVEC-DB1) 和淋巴 (HMVEC-DLy) 内皮细胞的混合群体。
成体细胞用“广告”表示；新生细胞用“Neo”表示。汇集和单一捐助者都可用。通过联系我们的科学支持团队，可以获得更多的捐赠者特征。内皮细胞和培养基使用方便且易于使用，使研究人员能够专注于结果。 Lonza 的细胞、培养基和试剂一起经过质量测试，并保证作为一个完整的细胞系统提供最佳性能。
冷冻保存的成人皮肤微血管内皮细胞在第三代运输。冷冻保存的真皮微血管内皮细胞保证通过 15 次群体倍增（CC-2543、CC-2505 和 CC-2516）和 12 次群体倍增（CC-2810、CC-2811、CC-2812 和 CC-2813），表达 CD31/105 ，von Williebrand 因子 VIII，对乙酰化低密度脂蛋白摄取呈阳性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。
Benefits
- Ready-To-Use
- No tissue dissection required
- Increased data reproducibility
- Characteristic morphology and staining tests confirm tissue origin and cell type
- Negative for mycoplasma, bacteria, yeast and fungi
- Certificate of Analysis (CoA) is available for every batch
Content & Storage
Content
1 x Cryopreserved Ampule with ≥500,000 cells/vial
Safety Data Sheets (SDS)
View SDS
SDS – Dutch
SDS – German
SDS – Italian
SDS – Spanish
SDS – French
SDS – English
SDS – Czech
Related Products
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Data
HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult
Human microvascular endothelial cells (CC-2543) were stained for von Willebrand Factor using antibody (Sigma, F3520) at 1:200 dilution (green). Secondary antibody used was VECTDI1088 DyLight 488 (Vector Laboratories). To detect CD31/PECAM-1, cells were stained with (R&D Systems, BBA7) at 1:100 dilution(red). Secondary antibody used was VECTDI2594 DyLight 594 (Vector Laboratories).
使用稀释度为 1:200（绿色）的抗体（Sigma，F3520）对人微血管内皮细胞（CC-2543）进行血管性血友病因子染色。 使用的二抗是 VECTDI1088 DyLight 488 (Vector Laboratories)。 为了检测 CD31/PECAM-1，细胞以 1:100 稀释度（红色）用（R&D Systems，BBA7）染色。 使用的二抗是 VECTDI2594 DyLight 594 (Vector Laboratories)。
HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult
Human microvascular endothelial cells (CC-2543) were stained for von Willebrand Factor using antibody (Sigma, F3520) at 1:200 dilution (green). Secondary antibody used was VECTDI1088 DyLight 488 (Vector Laboratories). To detect CD31/PECAM-1, cells were stained with (R&D Systems, BBA7) at 1:100 dilution(red). Secondary antibody used was VECTDI2594 DyLight 594 (Vector Laboratories).
使用稀释度为 1:200（绿色）的抗体（Sigma，F3520）对人微血管内皮细胞（CC-2543）进行血管性血友病因子染色。 使用的二抗是 VECTDI1088 DyLight 488 (Vector Laboratories)。 为了检测 CD31/PECAM-1，细胞以 1:100 稀释度（红色）用（R&D Systems，BBA7）染色。 使用的二抗是 VECTDI2594 DyLight 594 (Vector Laboratories)。
lonza,CC-3245,SkGM™-2 骨骼肌细胞生长培养基-2 BulletKit™
Catalog #: CC-3245
Culture system containing SkBMTM-2 Basal Medium (CC-3246) and SkGMTM-2 SingleQuotsTM supplements (CC-3244) required for growth of Skeletal Muscle Myoblasts.
Product Overview
我们提供两种骨骼肌生长培养基 – SkGMTM 和 SkGMTM-2。
SkGMTM-2 骨骼肌细胞生长培养基-2 BulletKitTM (CC-3245) 是 SkGMTM 培养基的增强版。 胎球蛋白已被去除，基础培养基也得到了进一步优化。
SkGMTM BulletKitTM (CC-3160) 针对人体骨骼肌细胞进行了优化。 SkGMTM -2 专为与正常和糖尿病骨骼肌成肌细胞一起使用而设计
Content & Storage
Content
1 x SkBMTM-2 Basal Medium (CC-3246), 500 mL
1 x SkGMTM-2 SingleQuotsTM Supplement Pack (CC-3244) containing:
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Natural Cap Vial with Dexamethasone, 0.50 mL
1 x Bottle FBS, 50.00 mL1 x Bottle L-Glutamine, 10.00 mL
Related Products
SKBM™- 2 Skeletal Muscle Cell Growth Basal Medium-2 CC-3246
SKBM™- 2 骨骼肌细胞生长基础培养基-2 CC-3246
SKGM™ – 2 Skeletal Muscle Cell Growth Medium-2 SingleQuots™ Supplements and Growth Factors CC-3244
SKGM™ – 2 种骨骼肌细胞生长培养基-2 SingleQuots™ 补充剂和生长因子 CC-3244
HSMM – Human Skeletal Muscle Myoblasts CC-2580
D-HSMM – Diseased Human Skeletal Muscle Myoblasts – Diabetes Type I CC-2900
D-HSMM – 患病的人体骨骼肌成肌细胞 – I 型糖尿病 CC-2900
D-HSMM – Diseased Human Skeletal Muscle Myoblasts – Diabetes Type II CC-2901
D-HSMM – 患病的人体骨骼肌成肌细胞 – II 型糖尿病 CC-2901
lonza,PT-2599,NHNP-人类神经祖细胞
Catalog #: PT-2599
Cryopreserved ampule of Normal Human Neural Progenitor (NHNP) cells containing ≥1.2 million cells
正常人神经祖细胞 (NHNP) 细胞的冷冻保存安瓿，含有 ≥ 120 万个细胞
Product Overview
PoieticsTM 神经祖细胞 (NHNP) 是从人脑皮层中分离出来的冷冻保存的神经球。神经球是通常称为神经干细胞和祖细胞 (NSPC) 或人中脑衍生的神经祖细胞 (hmNPC) 的细胞簇。这些神经球可以作为 3D 聚集体在悬浮液中保持未分化。为了诱导分化，可以将神经球转移到具有脑源性神经营养 (BDNF) 的层粘连蛋白涂层血管中，这将诱导分化为神经元、星形胶质细胞和少突胶质细胞。
冷冻保存的 PoieticsTM 人类神经祖细胞 (NHNP) 保证每个安瓿含有 ≥ 120 万个活细胞，并且在分化后对神经元 II 类 β 微管蛋白和胶质纤维酸蛋白 (GFAP) 检测呈阳性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。推荐培养基：NPMMTM Neural Progenitor Maintenance BulletKitTM 培养基，用于从冷冻保存中恢复并作为神经球进行维持； NPDMTM 神经祖细胞分化 BulletKitTM 培养基 + 25 ng/ml 脑源性神经营养因子 (BDNF)，用于将神经球非定向分化为神经元、星形胶质细胞和少突胶质细胞。
只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
Benefits
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis provided for every cell lot
Applications
- Drug development
- Neurotoxicity
- Neurogenesis and CNS function
- Neurotransmitter disorders
- Electrophysiology
Content & Storage
Content
1 x Cryopreserved ampule NHNP containing ≥ 1.2 million cells
Related Products
NPMM™ Neural Progenitor Maintenance Medium BulletKit™ CC-3209
NPMM™ 神经祖细胞维护培养基 BulletKit™ CC-3209
NPBM™ Neural Progenitor Basal Medium CC-3210
Neural Progenitor SingleQuots™ Supplements CC-4242
NPDM™ Neural Progenitor Differentiation Medium BulletKit™ CC-3229
NPDM™ 神经祖细胞分化培养基 BulletKit™ CC-3229
NHA – Human Astrocytes CC-2565
NHA – 人类星形胶质细胞 CC-2565
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218
lonza,00194987,H-RPE -人视网膜色素上皮细胞
Catalog #: 00194987
DISCONTINUED PRODUCT
Cryopreserved ampule of Human Retinal Pigment Epithelial Cells (H-RPE) containing ≥500,000 cells.
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Product Overview
The human retinal pigment epithelium (H-RPE) consists of pigmented cells situated between the neuroretina and the choroids. The H-RPE is comprised of hexagonal cells organized into a monolayer that is densely packed with pigment granules. The H-RPE is of the neuroectodermal origin and is therefore considered to be part of the retina. H-RPE cells play a critical role in visual function and photoreceptor viability.
Lonza’s H-RPE (Human Retinal Pigment Epithelial) cells are cryopreserved, primary cells that are packaged at passage 2 and contain ≥500,000 cells per vial. H-RPE are guaranteed through 5 population doublings and stain positive (≥90%) for pancytokeratin and Zo-1 and negative (≤10%) for fibroblast contamination and endothelial marker CD31 ( ≤1%). They also test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.
人视网膜色素上皮 (H-RPE) 由位于神经视网膜和脉络膜之间的色素细胞组成。 H-RPE 由六边形细胞组成，这些细胞组织成一个单层，其中密集地填充着颜料颗粒。 H-RPE 起源于神经外胚层，因此被认为是视网膜的一部分。 H-RPE 细胞在视觉功能和感光细胞活力方面发挥着关键作用。
Lonza 的 H-RPE（人视网膜色素上皮细胞）细胞是冷冻保存的原代细胞，在第 2 代时包装，每瓶含有 ≥500,000 个细胞。 H-RPE 通过 5 次群体倍增和泛细胞角蛋白和 Zo-1 染色阳性 (≥90%) 和成纤维细胞污染和内皮标志物 CD31 (≤1%) 染色阴性 (≤10%) 得到保证。 他们还检测出支原体、细菌、酵母和真菌呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书 (CoA)。
Benefits
- Guaranteed through 5 population doublings
- Test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- A Certificate ofAnalysis is provided for each cell lot purchased
Applications
- Age-related macular degeneration
- Retinitis pigmentosa
- Diabetic retinopathy
- Photoreceptor degeneration
- Gene expression
- Cellular interactions
Content & Storage
Content
1 x Cryopreserved ampule of H-RPE containing ≥500,000 cells
Instructions, SDS, CoA
Related Products
RtEGM™ Retinal Pigment Epithelial Cell Growth Medium BulletKit™ 00195409
RtEGM™ 视网膜色素上皮细胞生长培养基 BulletKit™ 00195409
lonza,原代细胞和干细胞,NoSpinTM HepaRGTM
Catalog #: NSHPRG
Fully differentiated HepaRGTM cells supplied in a format that does not require centrifugation following thaw. Each ampule contains ≥8.0 million cells.
Product Overview
HepaRGTM Cells are fully-functional, adult-phenotype, human hepatic cells. HepaRGTM Cells exhibit many characteristics similar to primary human hepatocytes and can be used for many of the same applications. The increased metabolic activity of HepaRG Cells compared to HepG2 enables more predictive value of higher throughput liver toxicity assays conducted in early drug discovery.
The NoSpin HepaRGTM Cells are HepaRG Cells that are provided in a convenient, terminally differentiated, cryopreserved format that does not require the user to centrifuge, resuspend, or count the cells after thawing. This eliminates multiple steps that introduce qualitative and quantitative variability and benefits high-volume labs by reducing the time and effort needed to perform an assay.
Each lot has consistent yield, viability, and functionality across multiple applications.
Using HepaRGTM Cells has never been easier!
Note: The NoSpin HepaRGTM Cells are fully differentiated and non-proliferating. For the proliferating and non-differentiated version of HepaRGTM Cells, please contact Biopredic International directly.
HepaRGTM 细胞是功能齐全的成人表型人类肝细胞。 HepaRGTM 细胞表现出许多与原代人肝细胞相似的特性，可用于许多相同的应用。与 HepG2 相比，HepaRG 细胞增加的代谢活性使得在早期药物发现中进行的更高通量肝毒性测定具有更高的预测价值。
NoSpin HepaRGTM 细胞是 HepaRG 细胞，以方便的、最终分化的、冷冻保存的形式提供，无需用户在解冻后离心、重悬或计数细胞。这消除了引入定性和定量可变性的多个步骤，并通过减少执行检测所需的时间和精力来使大容量实验室受益。
每个批次在多个应用程序中都具有一致的产量、可行性和功能。
使用 HepaRGTM 细胞从未如此简单！
注意：NoSpin HepaRGTM 细胞是完全分化且不增殖的。对于 HepaRGTM 细胞的增殖和非分化版本，请直接联系 Biopredic International。
Benefits
- Hepatoctye-like morphology and function
- Widely accepted as screening tool for liver toxicity
- No centrifugation step required for higher throughput
- Greater metabolic activity than HepG2 cells
Applications
- Drug metabolism
- infectious disease
- ADMETox
Content & Storage
Content
1 x Cryopreserved ampule NoSpinTM HepaRGTM containing ≥8 million cells
Data
HepaRG CYP Induction chart
Chart displaying cyp induction of HepaRG cells
The importance of cell-subtype selection in creation of a 3D liver fibrosis model
Data sheet detailing 3D liver fibrosis model in collaboration with Viskol
lonza,CC-2541,NHBE-不含维甲酸的人支气管/气管上皮细胞
Catalog #: CC-2541
Cryopreserved ampule of Normal Human Bronchial Epithelial (NHBE) cells without Retinoic Acid containing ≥500,000 cells
Product Overview
Normal and Diseased Bronchial Epithelial (NHBE and DHBE) cells are isolated from epithelial lining of airways above bifurcation of the lungs. Lonza’s Normal Human Bronchial Epithelial Cells (NHBE) without retinoic acid are available. Certain lots with Small Airway epithelial cells (CC-2547) or normal human lung fibroblasts (CC-2512) from matched donors are available.
Cryopreserved human bronchial epithelial cells without retinoic acid are guaranteed through 15 population doublings. Cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
正常和患病的支气管上皮（NHBE 和 DHBE）细胞是从肺分叉上方的气道上皮衬里分离出来的。 Lonza 提供不含维甲酸的正常人支气管上皮细胞 (NHBE)。 某些批次的小气道上皮细胞 (CC-2547) 或来自匹配供体的正常人肺成纤维细胞 (CC-2512) 可用。
不含维甲酸的冷冻保存的人支气管上皮细胞通过 15 次群体倍增得到保证。 细胞对支原体、细菌、酵母和真菌检测呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。
Select Applications with Lonza’s Human Bronchial Epithelial Cells –
- A Rotating Bioreactor for Scalable Culture and Differentiation of Respiratory Epithelium”. Respiratory epithelium is difficult to grow as it requires a well-maintained polarizing air‐liquid interface (ALI) to maintain differentiation. Raredon et al. describe a scalable and easy method to conduct air-liquid interface with Lonza’s bronchial epithelial cells guaranteed for ALI differentiation (Cat. No. CC-2540S).
- Cell concentration and space for growth are required for productive NHBE cell branching in vitro. Click to read this article by Hagiwara et.al that shows how co-cultures with HUVECs in 3D culture influence reconstruction of branched tubular structures in lung epithelial cells.
- Wu, et al. reports for the first time that HBE cells when grown in B-ALITMMedium can differentiate into 3D glandular acinar structures, likesalivary and mammary epithelial. The cells were grown on the basement membrane matrix Matrigel under select conditions and have shown to exhibit formation of brochospheres or tracheospheres with ciliated lining.
Benefits
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis provided for every cell lot
Content & Storage
Content
1 x Cryopreserved ampule containing ≥500,000 cells
Related Products
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540
NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞
NHBE – Human Bronchial/Tracheal Epithelial Cells for B-ALITM Culture CC-2540S
NHBE – 用于 B-ALITM 培养的人支气管/气管上皮细胞 CC-2540S
HMVEC-L – Human Lung Microvascular Endothelial Cells CC-2527
D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma for B-ALI™ Culture 00194911S
D-HBE-As – 患病的人支气管/气管上皮细胞 – B-ALI™ 培养的哮喘 00194911S
Data
Co-culture Experiments with Lonza’s Small Airway Epithelial Cells and SAGM™ BulletKit™ Media
Data Courtesy of Furukawa, et al. Lonza’s SAEC cells were co-cultured with primary normal mammary epithelial cells, breast cancer cell lines MCF-7 and MDA-MB-231 in Lonza’s SAGM™ BulletKit™ Growth Media. Breast cancer cell lines showed differences in morphology and proliferation rates in the presence of small airway epithelial cells. The image above shows phase contrast imaging with a fluorescent (red) overlay of MCF-7 cell line. MCF-7 cell lines survived as tight clusters in SAGM™ Media. When co-cultured with Lonza’s primary SAECs, MCF-7 cells demonstrated enhanced growth all the while staying clustered.
Co-culture Experiments with Lonza’s Bronchial Epithelial Cells and BEGM™ BulletKit™ Media
Furukawa, et al. demonstrates BEGM™ BulletKit™ is suitable for culturing Lonza’s NHBEs as well as to support co-cultures with lung epithelial cell line, BEAS-2B, and breast cancer cell lines, MCF-7 and MDA-MB-231. The breast cancer cell line showed differences in morphology and proliferation rates when cultured with primary NHBE cells vs. BEAS-2B cell line. The paper highlights the importance of using primary NHBE cells as a side-by-side control when conducting experiments with normal lung epithelial cell line such as BEAS-2B because the non-transformed primary cells can validate or offset the data generated using cell lines.
古川等人。 演示 BEGM™ BulletKit™ 适用于培养 Lonza 的 NHBE，并支持与肺上皮细胞系 BEAS-2B 和乳腺癌细胞系 MCF-7 和 MDA-MB-231 的共培养。 当与原代 NHBE 细胞与 BEAS-2B 细胞系一起培养时，乳腺癌细胞系在形态和增殖率方面表现出差异。 本文强调了在对正常肺上皮细胞系（如 BEAS-2B）进行实验时使用原代 NHBE 细胞作为并排对照的重要性，因为未转化的原代细胞可以验证或抵消使用细胞系生成的数据。
lonza,lonza 00192151,KBM™ Gold 角质形成细胞生长基础培养基
Catalog #: 00192151
KBM-GoldTM Keratinocyte Basal Medium for culture of human keratinocytes.
Product Overview
KBMTM Gold 是一种角质形成细胞基础培养基，单独出售。 这可以与 1 套 192152（角质形成细胞生长培养基 SingleQuotsTM 补充剂和生长因子）一起使用。 由基础培养基和补充剂组成的完整 KGMTM Gold BulletKitTM 可以使用目录号 192060 一起购买。
KGMTM Gold BulleKitTM 是一种新的稳健且多功能的无血清培养基配方，适用于高密度和低密度 NHEK-正常人表皮角质形成细胞培养
Content & Storage
Content
1 x Bottle Basal Medium 500 mL
Instructions, SDS, CoA
Related Products
NHEK-Ad – Human Epidermal Keratinocytes, Adult, Single Donor 00192627
NHEK-Ad – 人表皮角质形成细胞，成人，单一供体 00192627
NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled 00192906
NHEK-Neo – Human Epidermal Keratinocytes – Neonatal, Single Donor 00192907
KGM™ Gold Keratinocyte Growth Medium BulletKit™ 00192060
D-HEK-Ad – Diseased Human Epidermal Keratinocytes, Adult – Diabetes Type II CC-2926
D-HEK-Ad – 患病的人表皮角质形成细胞，成人 – II 型糖尿病 CC-2926
Data
Morphology of Lonza’s Adult Keratinocytes Grown in KGM™ Gold Growth Medium Compared to Competitor A’s Adult Keratinocytes in Their Medium.
The growth performance and longevity of Competitor A’s adult keratinocytes grown in Competitor A’s Medium were compared with Lonza’s adult keratinocytes grown in KGM-Gold™ Growth Medium. Although Competitor A claims ≥25 total population doublings for their adult keratinocytes, actual results show that this lot barely obtained 19 PD. Note the presence of several enlarged cells in Passage 2 in Competitor’s Medium. At the end of passage 4 (15 PD) Lonza’s adult keratinocytes consist of mostly small proliferative cells.
将竞争对手 A 的成年角质形成细胞在竞争对手 A 的培养基中生长的生长性能和寿命与龙沙在 KGM-Gold™ 生长培养基中生长的成年角质形成细胞进行了比较。 尽管竞争对手 A 声称其成年角质形成细胞的总种群倍增 ≥ 25，但实际结果表明，该批次几乎没有获得 19 个 PD。 注意竞争对手培养基中第 2 段中存在几个扩大的细胞。 在第 4 代（15 PD）结束时，Lonza 的成人角质形成细胞主要由小的增殖细胞组成。
lonza,lonza 2W-200,原代细胞和干细胞,人外周血 CD4+ T 细胞
Catalog #: 2W-200
Cryopreserved ampule of Human Peripheral Blood CD4+ T Cells containing ≥10 million cells, negatively selected.
Product Overview
CD4+ T cells are also known as helper T cells because they act on other cells of the immune system to promote various aspects of the immune response, including immunoglobulin isotype switching and affinity maturation of the antibody response, macrophage activation and enhanced activity of natural killer cells and cytotoxic T cells.
CD4+ T 细胞也被称为辅助 T 细胞，因为它们作用于免疫系统的其他细胞以促进免疫反应的各个方面，包括免疫球蛋白同种型转换和抗体反应的亲和力成熟、巨噬细胞活化和增强自然杀伤细胞的活性 和细胞毒性 T 细胞。
Cells You Can Count on to Perform
Lonza CD4+T Cells are isolated from peripheral blood of healthy donors using negative immunomagnetic separation. Cryopreserved CD4+ T cells are guaranteed ≥ 90% pure for CD4+ T cells, as assessed by flow cytometry. They are guaranteed to contain ≥ 10 million viable cells per vial. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Cells are available from multiple donors. Please contact Customer Service for details on available inventory and donor demographic characteristics (age, gender, and ethnicity).
Choices in Cell Type and Tissue Source
Hematopoietic and immune research requires cells from different donors and tissue sources. That’s why Lonza offers many different peripheral blood cell types. If you need different cell types from the same donor, you can either isolate from Lonza cryopreserved PBMCs or contact Customer Service to inquire about cells matched from the same donor. In addition, if you are interested in more naive T cells, unprocessed bone marrow, autologous bone marrow and whole blood from the same donor, bone marrow CD34+ cells, or cord blood CD34+ cells might be good solutions.
Supported by Lonza Media LGM-3TM
Lymphocyte Growth Medium is recommended for optimal performance. Please contact Scientific Support for thawing and culturing guidance. Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.
Benefits
– Test negative for mycoplasma, bacteria, yeast, and fungi
– cells from a variety of donors available
– Certificate of Analysis provided for each lot purchased
– HLA typing available
Content & Storage
Content
1 x Cryopreserved ampule of Human CD4+ T Cells containing ≥10 million cells
Related Products
Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012
P3 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-3012
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024
P3 原代细胞 4D-Nucleofector® X 试剂盒 L V4XP-3024
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032
P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096
P3 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-3096
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960
P3 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-3960
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218
lonza,lonza CC-2701,原代细胞和干细胞,NHDC-人类树突状细胞
Catalog #: CC-2701
Cryopreserved ampule of Normal Human Dendritic Cells (NHDC) containing ≥2.5 million cells
含有≥250 万个细胞的正常人树突状细胞 (NHDC) 冻存安瓿
Product Overview
树突状细胞 (DC) 通过处理来自病原体的抗原并将抗原呈递给 T 细胞以启动免疫反应，从而充当免疫系统的信使细胞。 PoieticsTM 正常人树突状细胞经过一组特定表面标志物的测试，包括 CD11c+、CD86+、CD80+。 HLADR+ 和 CD14- 提供细胞的详细表征。这些细胞可以在含有适当细胞因子（IL-4 和 GM-CSF）的培养物中存活长达 7 天，以将细胞维持为未成熟树突状细胞 (iDC)。通过添加 TNF-α 和 IL-1β，细胞可以进一步分化为成熟的树突状细胞。
应用： 抗原呈递和摄取 初级研究 药理学 药物诱导的细胞因子分泌免疫抑制
冷冻保存的 PoieticsTM 正常人树突状细胞 (NHDC) 保证每安瓿含有 ≥ 300 万个活细胞。对 CD80、CD86、HLA-DR、CD11c 和 CD14 的表达水平进行了测试，仅供参考。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。推荐培养基：LGM-3TM 淋巴细胞生长培养基 + 50 ng/ml GM-CSF + 50 ng/ml IL-4
只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
Benefits
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis provided for every cell lot
Content & Storage
Content
1 x Cryopreserved ampule of NHDC containing ≥2.5 million cells
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Unprocessed Human Bone Marrow, Fresh, 10 mL 1M-105
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lonza,lonza CC-2550,原代细胞和干细胞,NHAC-kn-人膝关节软骨细胞
Catalog #: CC-2550
Cryopreserved ampule of Normal Human Knee Articular Chondrocytes (NHAC-kn) containing ≥ 750,000 cells
Product Overview
CloneticsTM Normal Human Articular Chondrocytes (NHAC-kn) are isolated from tissue taken from the human knee.
Cryopreserved CloneticsTM Normal Human Articular Chondrocytes are guaranteed through 15 population doublings. After differentiation, they are guaranteed positive for collagen type II and sulfated proteoglycans. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
CloneticsTM 正常人关节软骨细胞 (NHAC-kn) 是从取自人膝关节的组织中分离出来的。
冷冻保存的 CloneticsTM 正常人关节软骨细胞通过 15 次群体倍增得到保证。 分化后，保证它们对 II 型胶原蛋白和硫酸化蛋白多糖呈阳性反应。 所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。
Recommended medium:
- CGMTM Chondrocyte Growth Medium for expansion
- CDMTM Chondrocyte Differentiation Medium for differentiation of human chondrocytes
Articular cartilage is characterized by high wear resistance and poor regenerative qualities. It is responsible for much of the compressive resistance and load bearing qualities of the knee joint.
Applications
- Osteoarthritis
- Cartilage failure
- Cellular matrix linkage rupture
- Chondrocyte protein synthesis inhibition
- Chondrocyte apoptosis
Content & Storage
Content
1 x Cryopreserved ampule of NHAC-kn containing ≥750,000 cells
Instructions, SDS, CoA
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P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012
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lonza,lonza CC-2538,原代细胞和干细胞,NHOst-Human Osteoblasts
Catalog #: CC-2538
Cryopreserved ampule of Normal Human Osteoblasts (NHOst) containing ≥ 500,000 cell
Product Overview
Cryopreserved CloneticsTM Normal Human Osteobalsts (NHOst) are guaranteed through 10 population doublings, stain positive for alkaline phosphatase and mineralization.
All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
冷冻保存的 CloneticsTM 正常人骨剂 (NHOst) 可通过 10 次种群倍增、碱性磷酸酶和矿化染色呈阳性来保证。
所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。
Benefits
- Ready-To-Use
- No tissue dissection required
- Increased data reproducibility
- Characteristic morphology and staining tests confirm tissue origin and cell type
- Negative for mycoplasma, bacteria, yeast and fungi
- Certificate of Analysis (CoA) is available for every batch
Content & Storage
Content
1 x Cryopreserved ampule of NHOst containing ≥ 500,000 cells
Instructions, SDS, CoA
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lonza,lonza PT-3004,人间充质干细胞 (hMSC) 脂肪分化培养基 BulletKitTM
Catalog #: PT-3004
Culture system containing basal medium and supplements required for maintenance and induction of hMSC adipogenic differentiation
Product Overview
Stem cells possess the unique ability to differentiate into multiple lineages of cells including adipocytes. Lonza’s hMSC Adipogenic Differentiation BulletKitTM Medium is a serum containing media system designed to induce adipogenic differentiation of bone marrow derived hMSC into mature, functionally active adipocytes in as few as 19 days. Differentiation can then be validated by a variety of methods including the use of Lonza’s AdipoRedTM Adipogenic Assay Reagent which provides a quantitative value related to the amount of adipogenic differentiation.
干细胞具有分化成包括脂肪细胞在内的多种细胞谱系的独特能力。 Lonza 的 hMSC 成脂分化 BulletKitTM 培养基是一种含血清的培养基系统，旨在在短短 19 天内诱导骨髓来源的 hMSC 成脂分化成成熟的、功能活跃的脂肪细胞。 然后可以通过多种方法验证分化，包括使用 Lonza 的 AdipoRedTM 脂肪生成分析试剂，该试剂提供与脂肪生成分化量相关的定量值。
Partial List of Cells Differentiated with hMSC Adipogenic Differentiation BulletKitTM Medium*:
- Human bone marrow derived stem cells
- C57BL/6 mice bone marrow derived stem cells
- human adipose derived stem cells
- Human periodontal ligament derived stem cells
- Human hamstring tendon derived stem cells
- hMSC 成脂分化 BulletKitTM 培养基提供诱导培养基和维持培养基，保证诱导骨髓来源的 hMSC 成脂分化成成熟的、功能活跃的脂肪细胞；每瓶 hMSC 脂肪分化 BulletKitTM 培养基都含有足够的诱导培养基和维持培养基，可以将大约 300 万个骨髓来源的 hMSC [大约 15 个孔，以 6 孔板形式] 完全分化成成熟的功能活跃的脂肪细胞。hMSC 脂肪分化培养基以 BulletKitTM 培养基（目录号 PT-3004）的形式提供，其中包括用于诱导和维持的基础培养基以及用于脂肪形成诱导和维持骨髓来源的 hMSCs 的必要补充剂。只有在专门使用适当的培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。如果您在选择合适的培养基、试剂或方案方面需要帮助，请联系 Lonza Scientific Support。
*从客户数据生成的单元格列表。 Lonza 仅常规地将这种培养基与 Lonza 的人骨髓来源的间充质干细胞一起使用，因此只能保证这种培养基与这些细胞一起成功使用。
Content & Storage
Content
1 x hMSC Adipogenic Maintenance Medium (PT-3102A)
1 x hMSC Adipogenic Induction Medium (PT-3102B)
1 x hMSC Adipogenic Maintenance SingelQuot Kit (PT-4122)
1 x hMSC Adipogenic Induction SingleQuot Kit (PT-4135)
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lonza,CC-7231,DHLF-IPF-患病的人肺成纤维细胞特发性肺纤维化
Catalog #: CC-7231
冷冻保存的患病人肺成纤维细胞、特发性肺纤维化 (DHLF-IPF) 安瓿，含有 ≥ 500,000 个细胞
Product Overview
IPF (Idiopathic Pulmonary Fibrosis) is a fatal disease that usually affects adults between the ages of 50-70. The disease is characterized by progressive decline in lung function resulting from scarring of lung tissue.
IPF varies from person to person where in some, fibrosis happens quickly, while the process can be much slower in others. IPF has no cure yet and both geneticand environmental factors are attributed to the development of the disease. Genetics may play a role in causing IPF, and cigarette smoking is the most accepted risk factor in increasing the chances of developing IPF. Recent research has helped doctors understand the disease more closely so they can more quickly diagnose it than in the past. Efforts are still underway in the research community to understand the pathways of disease progression and subsequently develop cures for IPF. IPF was once thought to be a chronic inflammatory process but recent evidence suggests that the abnormal activation of alveolar epithelial cells (AECs) induce over-activation of fibrotic response. The fibroblast and myofibroblast foci secrete excessive amounts of collagens, leading to scarringand destruction of the lung architecture. The mechanisms that link IPF with ageing and aberrant epithelial activation are unknown.
To understand the progression of IPF disease, Lonza offers cryopreserved lung fibroblasts isolated from donors diagnosed with IPF. Cryopreserved IPF fibroblasts are shipped at passage 2 and stain negative for von Willebrand factor VIII, cytokeratins 14, 18 and 19, and alpha smooth muscle actin. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
IPF（特发性肺纤维化）是一种致命疾病，通常影响 50-70 岁的成年人。该疾病的特征是肺组织瘢痕形成导致肺功能进行性下降。
IPF 因人而异，在某些人中，纤维化发生得很快，而在另一些人中该过程可能要慢得多。 IPF 还没有治愈方法，遗传和环境因素都归因于疾病的发展。遗传可能在导致 IPF 中起作用，而吸烟是增加 IPF 发生机会的最公认的风险因素。最近的研究帮助医生更深入地了解这种疾病，因此他们可以比过去更快地诊断它。研究界仍在努力了解疾病进展的途径并随后开发 IPF 的治疗方法。 IPF 曾被认为是一种慢性炎症过程，但最近的证据表明，肺泡上皮细胞 (AEC) 的异常激活会导致纤维化反应的过度激活。成纤维细胞和肌成纤维细胞病灶分泌过量的胶原蛋白，导致肺结构的瘢痕形成和破坏。将 IPF 与衰老和异常上皮激活联系起来的机制尚不清楚。
为了了解 IPF 疾病的进展，Lonza 提供了从诊断为 IPF 的供体中分离出来的冷冻保存的肺成纤维细胞。冷冻保存的 IPF 成纤维细胞在第 2 代运输，并且对血管性血友病因子 VIII、细胞角蛋白 14、18 和 19 以及 α 平滑肌肌动蛋白染色呈阴性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。
Relevant Downloads (Lonza’s Normal Human Lung Fibroblasts for IPF Research):
- Lung Epithelium Overexpressed Noncoding RNA (LEON): Role of a Novel Long Intergenic Noncoding RNA in Idiopathic Pulmonary Fibrosis
- The IL-33/ST2 Axis Is Upregulated in Fibrotic Lung Disease
- Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) promotes lung fibroblast proliferation, survival and differentiation to myofibroblasts
Benefits
- Gene expression pathways
- Phenotypic changes to compounds
- Transfect diseased cells
- Co-culture with immune cells
- Airway remodeling studies
- Cellular interaction
Content & Storage
Content
1 x Cryopreserved ampule of DHLF-IPF containing ≥500,000 cells
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lonza,PT-3003,人间充质干细胞 (hMSC) 软骨分化培养基 BulletKitTM
Culture system containing hMSC Chondrogenic Basal Medium (PT-3925) and hMSC Chondrogenic SingleQuotsTM Kit Supplement (PT-4121) required for hMSC chondrogenic differentiation
lonza,PT-3003,人间充质干细胞 (hMSC) 软骨分化培养基 BulletKitTM
Product Overview
Stem cells possess the unique ability to differentiate into multiple lineages of cells including chondrocytes. Lonza’s hMSC Chondrogenic Differentiation BulletKitTM Medium is a serum containing medium designed to induce chondrogenic differentiation, with the addition of TGF-β3, of human bone marrow derived mesenchymal stem cells into mature, functionally active chondrocytes in as few as 14 days. Differentiation can then be validated by a variety of methods including staining with alcian blue or safranin.
干细胞具有分化成包括软骨细胞在内的多种细胞谱系的独特能力。 Lonza 的 hMSC 软骨分化 BulletKitTM 培养基是一种含有血清的培养基，旨在通过添加 TGF-β3 诱导人骨髓来源的间充质干细胞在短短 14 天内向成熟、功能活跃的软骨细胞分化。 然后可以通过多种方法验证分化，包括用爱新蓝或番红染色。
Partial List of Cells Differentiated with hMSC Chondrogenic Differentiation BulletKitTM Medium*:
- Human bone marrow derived stem cells
- Human umbilical cord blood derived stem cells
- Human umbilical cord matrix derived stem cells
- C57BL/6 mice bone marrow derived stem cells
- human adipose derived stem cells
- Human hamstring tendon derived stem cells
添加 TGF-β3（单独出售）的 hMSC 软骨分化 BulletKitTM 培养基提供了一种分化培养基，可保证诱导骨髓来源的 hMSC 分化为成熟的功能活跃的软骨细胞；每瓶 hMSC 软骨分化 BulletKitTM 培养基都含有足够的分化培养基，可将至少 200 万个骨髓来源的 hMSC [大约 4 个完整的 24 孔板] 完全分化为成熟的功能活跃的软骨细胞。
hMSC 软骨分化培养基以 BulletKitTM 培养基（目录号 PT-3003）的形式提供，其中包括基础培养基和必要的补充剂（添加 TGF-β3 [单独出售，目录号 PT-4124]），用于骨髓来源的 hMSCs 的成软骨分化。基础培养基（Differentiation Basal Medium – Chondrogenic；目录号 PT-3925）和必要的补充剂（HMSC SQ 试剂盒 – 软骨；目录号 PT-4121 和用于 HMSC Chondro diff 的 TGF Beta 3；目录号 PT-4124）也可以单独购买。
只有在专门使用适当的培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。如果您在选择合适的培养基、试剂或方案方面需要帮助，请联系 Lonza Scientific Support。
*从客户数据生成的单元格列表。 Lonza 仅常规地将这种培养基与 Lonza 的人骨髓来源的间充质干细胞一起使用，因此只能保证这种培养基与这些细胞一起成功使用。
Content & Storage
Content
1 x hMSC Chondrogenic Basal Medium (PT-3925)
1 x hMSC Chondrogenic SingleQuot Kit (PT-4121)
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人间充质干细胞 (hMSC) 成骨分化培养基 BulletKitTM PT-3002
Human Mesenchymal Stem Cell (hMSC) Adipogenic Differentiation Medium BulletKitTM PT-3004
人间充质干细胞 (hMSC) 脂肪分化培养基 BulletKitTM PT-3004
Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5007
Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5008
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DPSC – Human Dental Pulp Stem Cells PT-5025
NHAC-kn – Human Knee Articular Chondrocytes CC-2550
NHAC-kn – 人膝关节软骨细胞 CC-2550
CGM™ Chondrocyte Growth Medium BulletKit™ CC-3216
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Chondrocyte ReagentPack™ Subculture Reagents CC-3233
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lonza,CC-2530, CC-2913,HPAEC-人肺动脉内皮细胞
Catalog #: CC-2530
含有 ≥ 500,000 个细胞的正常人肺动脉内皮细胞 (HPAEC) 冻存安瓿
Product Overview
Lonza’s Human Pulmonary Artery Endothelial Cells (HPAEC) and media are convenient and easy to use, allowing the researcher to focus on results. Cryopreserved HPAEC cells are shipped in third passage. Proliferating cells are shipped in fourth passage. Cells isolated from Diabetes Type 1 and 2 are also available.
CloneticsTM cells, medium and reagents are quality tested together and guaranteed to give optimum performance as a complete cell system.
Lonza 的人肺动脉内皮细胞 (HPAEC) 和培养基使用方便且易于使用，使研究人员能够专注于结果。 冷冻保存的 HPAEC 细胞在第三代运输。 增殖细胞在第四代运输。 也可使用从 1 型和 2 型糖尿病中分离的细胞。
CloneticsTM 细胞、培养基和试剂一起经过质量测试，保证作为一个完整的细胞系统提供最佳性能。
Benefits
- Assured for experimental use up to 15 population doublings
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Characterized by morphological observation throughout serial passage
- Certificate of Analysis provided for every cell lot
Applications
- Hypertension
- Arteriosclerosis
- Inflammation
- Oncology
- Drug screening
Content & Storage
Content
1 x Cryopreserved ampule of HPAEC containing ≥ 500,000 cells
Related Products
D-PASMC – Diseased Human Pulmonary Artery Smooth Muscle Cells – Diabetes Type II CC-2913
D-PASMC – 患病的人肺动脉平滑肌细胞 – II 型糖尿病 CC-2913
D-PASMC – Diseased Human Pulmonary Artery Smooth Muscle Cells – Diabetes Type I CC-2915
HMVEC-L – Human Lung Microvascular Endothelial Cells CC-2527
SAEC – Human Small Airway Epithelial Cells CC-2547
NHLF – Human Lung Fibroblasts CC-2512
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540
BSMC – Human Bronchial Smooth Muscle Cells CC-2576
EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™ CC-3162
P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5012
P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5024
P5 Primary Cell 4D-Nucleofector™ X Kit S V4XP-5032
P5 Primary Cell 96-well Nucleofector™ Kit V4SP-5096
P5 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-5960
P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5002
P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5010
Data
HPAEC – Human Pulmonary Artery Endothelial Cells
Human pulmonary artery endothelial cells (CC-2530) were immunohistochemistry labeled with von Willebrand Factor antibody (Sigma, F3520) used at 1:200. Secondary antibody used was VECTDI1088 DyLight 488 from (Vector Laboratories). Staining for CD31/PECAM-1 was conducted using antibody from R&D Systems, BBA7 at 1:100. Secondary antibody used to detect CD31/PECAM-1 was VECTDI2594 DyLight 594 (Vector Laboratories). Nuclei were stained with Hoechst.
HPAEC – Human Pulmonary Artery Endothelial Cells
Human pulmonary artery endothelial cells (CC-2530) were immunohistochemistry labeled with von Willebrand Factor antibody (Sigma, F3520) used at 1:200. Secondary antibody used was VECTDI1088 DyLight 488 from (Vector Laboratories). Staining for CD31/PECAM-1 was conducted using antibody from R&D Systems, BBA7 at 1:100. Secondary antibody used to detect CD31/PECAM-1 was VECTDI2594 DyLight 594 (Vector Laboratories). Nuclei were stained with Hoechst.
用 1:200 使用的血管性血友病因子抗体 (Sigma, F3520) 对人肺动脉内皮细胞 (CC-2530) 进行免疫组织化学标记。 使用的二抗是来自 (Vector Laboratories) 的 VECTDI1088 DyLight 488。 使用来自 R&D Systems 的抗体 BBA7 以 1:100 进行 CD31/PECAM-1 染色。 用于检测 CD31/PECAM-1 的二抗是 VECTDI2594 DyLight 594 (Vector Laboratories)。 细胞核用赫斯特染色。
lonza,lonza CC-4133,EGMTM 内皮细胞生长培养基 SingleQuotsTM 试剂盒
Catalog #: CC-4133
Supplements and growth factors required for culture of Endothelial Cells (basal media sold separately
Product Overview
EGMTM SingleQuotTM Kit are components sold separately and does not include the basal medium. These are part of the EGMTM Endothelial Cell Growth Medium BulletKitTM (Catalog Number CC-3124) which consists of basal medium and supplements packaged together.
Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used to grow CloneticsTM Primary Cells.
EGMTM SingleQuotTM 试剂盒是单独出售的组件，不包括基础培养基。 这些是 EGMTM 内皮细胞生长培养基 BulletKitTM（目录号 CC-3124）的一部分，它由包装在一起的基础培养基和补充剂组成。
从多种内皮细胞生长培养基中进行选择，以轻松培养原代内皮细胞。 每种内皮细胞生长培养基都有其特殊的配方，并针对某些细胞类型进行了优化。 每种类型的内皮细胞生长培养基在用于培养 CloneticsTM 原代细胞时都能保证发挥作用。
The following Endothelial Cell Growth Medium BulletKitsTM are available:
- EGMTM BulletKitTM (CC-3124) – Optimized for HUVECs cat. no. CC-2517 and CC-2519, contains 2% FBS and BBE. Does not contain exogenous VEGF.
- EGMTM-Plus BulletKitTM (CC-5035) – Enhanced version of EGMTM Medium. Optimized for HUVECs cat. no. CC-2935. Contains 2% FBS and BBE. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGMTMMedium.
- EGMTM-2 BulletKitTM (CC-3162) – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Contains 2% FBS and VEGF for rapid proliferation. Does not contain BBE.
- EGMTM-2 MV BulletKitTM (CC-3202) – Coronary and Iliac artery, human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. Does not contain BBE.
Lonza guarantees the performance of CloneticsTM/PoieticsTM Cells only if appropriate CloneticsTM/PoieticsTM Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.
只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
Content & Storage
Content
1 x Green Cap Vial with hEGF 0.50 mL
1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 Bottle BBE 2.00 mL
1 x Blue Cap Vial with Ascorbic Acid 0.50 mL
1 Bottle FBS 10.00 mL
Related Products
HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™ CC-2517
HUVEC – EGM™ CC-2517 中的人脐静脉内皮细胞，单一供体
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™ CC-2519
HUVEC – 人脐静脉内皮细胞，汇集，在 EGM™ CC-2519 中
EGM™ Endothelial Cell Growth Medium BulletKit™ CC-3124
EGM™ 内皮细胞生长培养基 BulletKit™ CC-3124
lonza,HUCLS,原代细胞和干细胞,星状细胞人类冷冻保存第 1 代
Catalog #: HUCLS
Human Cryopreserved Stellate cells derived from liver
Product Overview
Stellate cells are a resident cell type of the liver primarily functioning to store retinoids. In response to liver damage, stellate cells rapidly lose the stored retinoids and differentiate into a proliferating fibroblast-like cell that begins depositing collagen matrix. This activity causes buildup of collagen in the liver eventually leading to cirrhosis.
Stellate cells can be isolated from liver tissue, enriched, and placed into cell culture. When placed into culture, stellate cells begin to immediately differentiate into the activated state.
Human Cryopreserved Stellate Cells, 100K cells/vial, are stellate cells enriched from human liver isolations and grown to >80% confluency and then cryopreserved.
Human Stellate Growth Medium (Catalog MCST250) is available to support optimal culture health and growth characteristics of stellate cells.
星状细胞是肝脏的一种常驻细胞类型，主要功能是储存类维生素A。 作为对肝损伤的反应，星状细胞迅速失去储存的类维生素A并分化成增殖的成纤维细胞样细胞，开始沉积胶原蛋白基质。 这种活动会导致肝脏中胶原蛋白的堆积，最终导致肝硬化。
星状细胞可以从肝组织中分离、富集并放入细胞培养物中。 当放入培养物中时，星状细胞开始立即分化成活化状态。
人冷冻保存的星状细胞，100K 细胞/瓶，是从人肝分离物中富集的星状细胞，并生长到 >80% 的汇合度，然后冷冻保存。
人星状生长培养基（目录 MCST250）可用于支持星状细胞的最佳培养健康和生长特性。
Characterization performed:
- Post-thaw plating to observe characteristic stellate morphology.
- Flow cytometry using antibodies for Vinculin, Vimentin, and α-SMA
Benefits
- Low Passage
- High activation potential
Applications
- ADMETox
- Liver Disease
- NASH
Content & Storage
Content
Cryo Vial
100,000 cells/ vial
lonza,CC-2904,原代细胞和干细胞,NHCF-V-人心室心脏成纤维细胞
Catalog #: CC-2904
Cryopreserved ampule of Normal Human Ventricular Cardiac Fibroblasts (NHCF-V) containing ≥ 500,000 cells
Product Overview
Human Cardiac-Related Fibroblasts include Aortic Adventitial Fibroblasts (AoAF) isolated from the tunica external of the ascending or descending aorta and cardiac fibroblasts (NHCF-A and -V) isolated from the atrium or ventricle. Cells are isolated from normal, adult heart tissue. Cryopreserved cells are shipped at passage 2.
CloneticsTM NHCF-V & -A are guaranteed through 5 population doublings and express ≥90% collagen I and ≤10% Von Willibrand factor VIII after thaw. CloneticsTM AoAF are guaranteed through 10 population doublings and are negative for alpha smooth muscle actin. All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.
人类心脏相关成纤维细胞包括从升主动脉或降主动脉外部的被膜分离的主动脉外膜成纤维细胞 (AoAF) 和从心房或心室分离的心脏成纤维细胞 (NHCF-A 和 -V)。 细胞是从正常的成人心脏组织中分离出来的。 冷冻保存的细胞在第 2 代运送。
CloneticsTM NHCF-V 和 -A 通过 5 次群体倍增得到保证，解冻后表达 ≥90% 的胶原蛋白 I 和 ≤10% 的 Von Willibrand 因子 VIII。 CloneticsTM AoAF 通过 10 次群体倍增得到保证，并且对 α 平滑肌肌动蛋白呈阴性。 所有细胞的支原体、细菌、酵母菌和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书 (CoA)。
Benefits
- Assured for experimental use up to 15 population doublings
- Express ≥90% collagen I and ≤10% Von Willibrand factor VIII
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis provided for every cell lot
Applications
- Vascular diseases
- Neointimal formation
- Vascular remodeling
- Atherosclerosis
- Hypertension
- Myocardial repair
- Extracellular maintenance during cardiac cell / tissue growth
- Cardiac Fibrosis Paracrine signalling
- Toxicology
- Gene expression
Content & Storage
Content
1 x Cryopreserved ampule NHCF-V containing ≥ 500,000 cells/vial
Related Products
AoAF – Human Aortic Adventitial Fibroblasts CC-7014
AoAF – 人主动脉外膜成纤维细胞 CC-7014
NHCF-A – Human Atrial Cardiac Fibroblasts CC-2903
HAEC – Human Aortic Endothelial Cells CC-2535
HAEC – 人主动脉内皮细胞 CC-2535
AoSMC – Human Aortic Smooth Muscle Cells CC-2571
AoSMC – 人主动脉平滑肌细胞 CC-2571
FGM™-3 Cardiac Fibroblast Growth Medium-3 BulletKit™ CC-4526
FGM™-3 心脏成纤维细胞生长培养基-3 BulletKit™ CC-4526
lonza,lonza CC-5022,原代细胞和干细胞,HEPES 缓冲盐溶液
Catalog #: CC-5022
HEPES Buffered Saline Solution, 100 mL
Overview
Product Overview
HEPES-Buffered Saline Solution (BSS), is used to neutralize the complex proteins in growth medium that may inactivate trypsin during trypsinization.
HEPES-BSS is a component of the ReagentPackTM Subculture kit (catalog number CC-5034). It is also available in a 500 mL size (catalog number CC-5024)
HEPES 缓冲盐溶液 (BSS) 用于中和生长培养基中的复杂蛋白质，这些蛋白质可能会在胰蛋白酶消化过程中使胰蛋白酶失活。
HEPES-BSS 是 ReagentPackTM Subculture 试剂盒（目录号 CC-5034）的一个组件。 它也有 500 mL 规格（目录号 CC-5024）
Content & Storage
Content
1 x Bottle HEPES Buffered Solution 100 mL
lonza,lonza CC-3166,CC-2555,CC-3165,PrEGM™ 前列腺上皮细胞生长培养基 BulletKit™
Catalog #: CC-3166
Culture system containing PrEBMTM Basal Medium (CC-3165) and PrEGMTM SingleQuotsTM Supplements (CC-4177) required for growth of Prostate Epithelial Cells
含有前列腺上皮细胞生长所需的 PrEBMTM 基础培养基 (CC-3165) 和 PrEGMTM SingleQuotsTM 补充剂 (CC-4177) 的培养系统
Product Overview
Prostate Epithelial Cell Growth Medium has been specially formulated for the maintenance and expansion of human prostate epithelial cells. The BulletKitTM consists of basal medium and supplements packaged together for convenience. PrEGMTM Medium contains no serum.
前列腺上皮细胞生长培养基专为人前列腺上皮细胞的维持和扩增而配制。 BulletKitTM 由基础培养基和补充剂组成，方便起见。 PrEGMTM 培养基不含血清。
Content & Storage
Content
1 x PrEBMTM Basal Medium (CC-3165), 500 mL
1 x PrEGMTM SingleQuotsTM Supplement Pack (CC-4177) containing:
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Amber Vial with Triiodothyronine, 0.50 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Natural Cap Vial with Transferrin, 0.50 mL
1 x Amber Vial with Epinephrine, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Amber Vial with Retinoic Acid, 0.50 mL
Related Products
PrEC – Human Prostate Epithelial Cells CC-2555
PrEC – 人前列腺上皮细胞 CC-2555
PrEBM™ Prostate Epithelial Cell Growth Basal Medium CC-3165
PrEBM™ 前列腺上皮细胞生长基础培养基 CC-3165
PrEGM™ Prostate Epithelial Cell Growth Medium SingleQuots™ Supplements and Growth Factors CC-4177
PrEGM™ 前列腺上皮细胞生长培养基 SingleQuots™ 补充剂和生长因子 CC-4177
lonza,CC-3111,原代细胞和干细胞,KGM™ 角质形成细胞生长培养基 BulletKit™
Catalog #: CC-3111
KGMTM Keratinocyte Growth Medium BulletKitTM culture system contains KBMTM Basal Medium (CC-3101) and KGMTM SingleQuot supplements (CC-4131) required for growth of human keratinocytes.
KGMTM 角质形成细胞生长培养基 BulletKitTM 培养系统包含人类角质形成细胞生长所需的 KBMTM 基础培养基 (CC-3101) 和 KGMTM SingleQuot 补充剂 (CC-4131)。
Product Overview
KGMTM Keratinocyte Growth Medium has been optimized for the proliferation of keratinocytes in a serum-free environment.
Did you know our customers have used KGMTM Media also to culture immortalized corneal epithelial cells? Check out Jolly, et. al. publication that provides some guidelines to do this.
KGMTM 角质形成细胞生长培养基已针对角质形成细胞在无血清环境中的增殖进行了优化。
您知道我们的客户也使用 KGMTM 培养基培养永生化角膜上皮细胞吗？ 看看 Jolly 等。 人。 提供了一些指导方针的出版物。
Content & Storage
Content
1 x KBMTM (CC-3101) Basal Medium, 500 mL
1 x KGMTM (CC-4131) Singlequot Supplement Pack containing:
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
lonza,lonza PT-5025,原代细胞和干细胞,DPSC-人类牙髓干细胞
Catalog #: PT-5025
Cryopreserved ampule of Human Dental Pulp Stem Cells containing ≥1 million cells
Product Overview
PoieticsTM 人类牙髓干细胞 (DPSC) 是从捐赠者的“智齿”拔牙过程中收集的成年第三磨牙中分离出来的。这些细胞表现出与骨髓来源的间充质干细胞非常相似的表型和功能特征。据报道，人类牙髓干细胞 (DPSC) 可分化出许多不同的谱系，包括软骨形成、成骨、脂肪形成和神经细胞。
人牙髓干细胞 (DPSC) 已在初级传代时冷冻保存，并且 DPSC 牙髓干细胞 BulletKitTM 培养基已针对细胞维持和扩增进行了优化。
冷冻保存的 PoieticsTM 牙髓干细胞 (DPSC) 保证通过 10 次群体倍增，表达 CD105、CD166、CD29、CD90 和 CD73，并且不表达 CD34、CD45 和 CD133。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。
Recommended Growth Media and Reagents
- DPSC Dental Pulp Stem Cell BulletKitTM Medium, PT-3005
The DPSC Dental Pulp Stem Cell BulletKitTM Medium is offered as a BulletKitTM Medium (catalog no. PT-3005) which includes both the basal media and the necessary supplements for proliferation of human dental pulp derived mesenchymal stem cells. The basal medium (DPSC Basal Medium; catalog no. PT-3927) and the necessary supplements (DPSC SingleQuot Kit; catalog no. PT-4516) can also be purchased individually.
Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.
DPSC 牙髓干细胞 BulletKitTM 培养基以 BulletKitTM 培养基（目录号 PT-3005）的形式提供，其中包括基础培养基和人牙髓衍生间充质干细胞增殖所需的补充剂。 基础培养基（DPSC 基础培养基；目录号 PT-3927）和必要的补充剂（DPSC SingleQuot 试剂盒；目录号 PT-4516）也可以单独购买。
只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
Applications
- Toxicology/drug screening
- Regenerative medicine/dentistry/cell therapy
- Muscular Dystrophy
- Corneal replacement therapy
- Cardiovascular disease
- Comparative stem cell research
Content & Storage
Content
1 x Cryopreserved ampule of Human Dental Pulp Stem Cells containing ≥1 million cells
Related Products
Human Dental Pulp Stem Cell Basal Medium PT-3927
Human Dental Pulp Stem Cell SingleQuots™ Supplements and Growth Factors PT-4516
人牙髓干细胞 SingleQuots™ 补充剂和生长因子 PT-4516
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218
hMSC – Human Mesenchymal Stem Cells PT-2501
hMSC – 人间充质干细胞 PT-2501
MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ PT-3001
MSCGM™ 间充质干细胞生长培养基 BulletKit™ PT-3001
Human Mesenchymal Stem Cell (hMSC) Osteogenic Differentiation Medium BulletKitTM PT-3002
人间充质干细胞 (hMSC) 成骨分化培养基 BulletKitTM PT-3002
Human Mesenchymal Stem Cell (hMSC) Chondrogenic Differentiation Medium BulletKitTM PT-3003
人间充质干细胞 (hMSC) 软骨分化培养基 BulletKitTM PT-3003
Human Mesenchymal Stem Cell (hMSC) Adipogenic Differentiation Medium BulletKitTM PT-3004
人间充质干细胞 (hMSC) 脂肪分化培养基 BulletKitTM PT-3004
TGF Beta 3 for hMSC Chondrogenic Differentiation Kit PT-4124
用于 hMSC 软骨分化试剂盒 PT-4124 的 TGF Beta 3
hADSC Human Adipose-Derived Stem Cells PT-5006
hADSC 人体脂肪干细胞 PT-5006
lonza,CC-4149,SmGM™-2 平滑肌细胞生长培养基-2 SingleQuots™ 补充剂和生长因子
Catalog #: CC-4149
SmGMTM-2 Smooth Muscle SingleQuotsTM Kit, Supplements and growth factors required for culture of Human Smooth Muscle Cells (basal media sold separately).
Product Overview
SmGMTM -2 SingleQuotTM 试剂盒是单独出售的组件，不包括基础培养基。 这些是 SmGMTM -2 平滑肌细胞生长培养基 BulletKitTM（目录号 CC-3182）的一部分。 由基础培养基和补充剂组成的完整 SmGMTM -2 BulletKitTM 可以使用目录号 CC-3182 一起购买。
SmGMTM-2 Smooth Muscle Growth Medium-2 是一种强大的培养基，用于维持和扩增来自各种组织来源的人类平滑肌细胞，包括心脏、气道、前列腺等。 该试剂盒还用于支持肠道细胞的维持。 SmGMTM-2 培养基含有 5% FBS。
CloneticsTM 培养基产品专为支持人和动物原代细胞的生长而设计。 媒体系统已针对各种细胞系统专门开发和优化。
Content & Storage
Content
1 x Lilac Cap Vial with Insulin 0.50 mL
1 x Gray Cap Vial with hFGF-B 1.00 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Bottle FBS 25.00 mL
1 x Green Cap Vial with hEGF 0.50 mL
Related Products
D-BSMC-As – Diseased Human Bronchial Smooth Muscle Cells – Asthma 00194850
D-BSMC-COPD – Diseased Human Bronchial Smooth Muscle Cells – COPD 00195274
BdSMC – Human Bladder Smooth Muscle Cells CC-2533
AoSMC – Human Aortic Smooth Muscle Cells CC-2571
UtSMC – Human Uterine Smooth Muscle Cells CC-2562
BSMC – Human Bronchial Smooth Muscle Cells CC-2576
UASMC – Human Umbilical Artery Smooth Muscle Cells CC-2579
PrSMC – Human Prostate Smooth Muscle Cells CC-2587
CASMC – Human Coronary Artery Smooth Muscle Cells CC-2583
InMyoFib – Human Intestinal Myofibroblasts CC-2902
D-PASMC – Diseased Human Pulmonary Artery Smooth Muscle Cells – Diabetes Type II CC-2913
D-PASMC – 患病的人肺动脉平滑肌细胞 – II 型糖尿病 CC-2913
D-AoSMC – Diseased Human Aortic Smooth Muscle Cells – Diabetes Type I CC-2914
D-AoSMC – 患病的人主动脉平滑肌细胞 – I 型糖尿病 CC-2914
D-PASMC – Diseased Human Pulmonary Artery Smooth Muscle Cells – Diabetes Type I CC-2915
D-AoSMC – Diseased Human Aortic Smooth Muscle Cells – Diabetes Type II CC-2916
D-CASMC – Diseased Human Coronary Artery Smooth Muscle Cells – Diabetes Type I CC-2917
D-CASMC – 患病的人冠状动脉平滑肌细胞 – I 型糖尿病 CC-2917
D-CASMC – Diseased Human Coronary Artery Smooth Muscle Cells – Diabetes Type II CC-2918
D-CASMC – 患病的人冠状动脉平滑肌细胞 – II 型糖尿病 CC-2918
lonza,lonza CC-2581,CC-2512,CC-2527,PASMC-人肺动脉平滑肌细胞
Catalog #: CC-2581
Cryopreserved ampule of Pulmonary Artery Smooth Muscle Cells (PASMC) containing ≥ 500,000 cells
含有 ≥ 500,000 个细胞的肺动脉平滑肌细胞 (PASMC) 冻存安瓿
Product Overview
Human Pulmonary Artery Smooth Muscle Cells (PASMC) are shipped in 3rd passage.
Human Pulmonary Artery Smooth Muscle Cells are guaranteed for 15 population doublings and to stain positive for alpha smooth muscle actin and negative for von Willebrand Factor VIII after differentiation.
All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.
人肺动脉平滑肌细胞 (PASMC) 在第 3 代运输。
人肺动脉平滑肌细胞保证 15 次群体倍增，分化后 α 平滑肌肌动蛋白染色呈阳性，von Willebrand 因子 VIII 染色呈阴性。
所有细胞的支原体、细菌、酵母菌和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书 (CoA)。
Applications
- Stroke
- Diabetes
- Vascular research
- Artherosclerosis
- Hypertension
Content & Storage
Content
1 x Cryopreserved ampule PASMC containing ≥500,000 cells/vial
Related Products
HMVEC-L – Human Lung Microvascular Endothelial Cells CC-2527
SAEC – Human Small Airway Epithelial Cells CC-2547
NHLF – Human Lung Fibroblasts CC-2512
NHLF – 人肺成纤维细胞 CC-2512
BSMC – Human Bronchial Smooth Muscle Cells CC-2576
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540
NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1012
P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1012
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1024
P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1024
P1 Primary Cell 4D-Nucleofector™ X Kit S V4XP-1032
P1 Primary Cell 96-well Nucleofector™ Kit V4SP-1096
P1 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-1960
P1 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-1960
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1002
P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1002
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1010
PASMC – Human Pulmonary Artery Smooth Muscle Cells
Human pulmonary artery smooth muscle cells(CC-2581) were stained with 1:1500 dilution of Anti Alpha-Smooth Muscle Actin dilution of (CBL171, Sigma). Immunofluorescence analysis was conducted using Secondary donkey polyclonal antibody Alexa Fluor Do anti Mo 594 Invitrogen (Thermo Fisher) A21203 at a 1:200 dilution. Nuclei were stained with Hoechst . In multi-well culture, PAMCSs appeared typically epitheloid (polygonal) forming monolayers of very low-level alpha-actin expressing cells. Rarely, a few intensely alpha-actin positive cells (arrows) were evident. These displayed distinct fusiform-elongated morphology.
PASMC – Human Pulmonary Artery Smooth Muscle Cells
Cells are isolated from human pulmonary artery and expanded prior to freezing (CC-2581). When plated & expanded, the cells express little to no alpha-actin unless first stimulated to differentiate.Cells were stained with Anti Alpha-Smooth Muscle Actin Chemicon (Millipore) CBL171 at 1:1500 dilution. Immunofluorescence analysis was conducted using Secondary donkey polyclonal antibody Alexa Fluor Do anti Mo 594 Invitrogen (Thermo Fisher) A21203 at 1:200 dilution. Nuclei were stained with Hoechst.
从人肺动脉中分离细胞并在冷冻前扩增 (CC-2581)。 当铺板和扩增时，除非首先刺激分化，否则细胞几乎不表达 α-肌动蛋白。细胞用 1:1500 稀释度的抗 α-平滑肌肌动蛋白化学 (Millipore) CBL171 染色。 使用 1:200 稀释度的第二驴多克隆抗体 Alexa Fluor Do 抗 Mo 594 Invitrogen (Thermo Fisher) A21203 进行免疫荧光分析。 细胞核用赫斯特染色。
lonza,CC-4461,R-CB-503,CC-3256,PNGM™ 原代神经元生长培养基 BulletKit™
Catalog #: CC-4461
含有 PNBMTM 基础培养基 (CC-3256) 和 PNGMTM SinglequotsTM 生长补充剂 (CC-4462) 的培养系统，是啮齿动物原代神经元生长所需的
Product Overview
CloneticsTM PNGMTM Primary Neuron Growth Medium Kit (PNGMTM) was specifically developed for culturing embryonic primary rat and mouse neurons. The media kit is recommended for use with all of our primary rat and mouse neurons.
CloneticsTM PNGMTM 原代神经元生长培养基试剂盒 (PNGMTM) 专为培养胚胎原代大鼠和小鼠神经元而开发。 建议将媒体套件与我们所有的主要大鼠和小鼠神经元一起使用。
Benefits
- Serum-free medium
- Allows for long-term maintenance and growth of neuronal cells
- Promotes development of high quality neuronal networks without the need of an astrocyte feeder layer
Content & Storage
Content
1 x PNBMTM Basal Medium (CC-3256), 200 mL
1 x PNGMTM SinglequotsTM Growth Supplement Pack (CC-4462)
Related Products
PNBMTM Primary Neuron Basal Medium, 200 mL CC-3256
PNGM™ Primary Neuron Growth Medium SingleQuots™ Supplements and Growth Factors CC-4462
Mouse C57 Brain Striatum Neurons M-CP-302
Mouse CD1 Brain Striatum Neurons M-CP-402
Mouse C57 Brain Cortex Neurons M-CX-300
Mouse CD1 Brain Cortex Neurons M-CX-400
Mouse Brain CD1 Hippocampus Neurons M-HI-401
Rat Cerebellar Granular Neurons R-CB-503
大鼠小脑颗粒神经元 R-CB-503
Rat Brain Striatum Neurons R-CP-502
Rat Brain Cortex Neurons R-CX-500
Rat Neonatal Dorsal Root Ganglion (DRG) Neurons R-DRG-505
大鼠新生儿背根神经节 (DRG) 神经元 R-DRG-505
Rat Brain Hippocampus Neurons R-HI-501
大鼠脑海马神经元 R-HI-501
Rat Brain Hypothalamus Neurons R-HTH-507
大鼠脑下丘脑神经元 R-HTH-507
ViaLightPlus Assay in PNGM™ Media of Rat Cortical Neurons
Comparison of survival of Clonetics™ Primary Rat Cortical Neurons cultured in PNGM™ Medium vs. Company A’s medium. Cryopreserved cells were thawed and plated in laminin-coated, 96 well plates at ~1000 cells per square mm (40% of the recommended seeding density) using either PNGM™ Medium or Company A’s medium. Cells were cultured without feeding for six days, at which time cell viability was assessed using the ViaLight™ Plus Cell Proliferation and Cytotoxicity BioAssay Kit. Results represent the average of 8 experiments.
在 PNGM™ 培养基与 A 公司培养基中培养的 Clonetics™ 原代大鼠皮质神经元的存活率比较。 使用 PNGM™ 培养基或 A 公司的培养基，将冷冻保存的细胞解冻并接种在层粘连蛋白涂层的 96 孔板中，每平方毫米约 1000 个细胞（推荐接种密度的 40%）。 细胞在不喂食的情况下培养六天，此时使用 ViaLight™ Plus 细胞增殖和细胞毒性生物测定试剂盒评估细胞活力。 结果代表 8 次实验的平均值。
lonza,R-DRG-505,CC-4461,R-EDRG-515,大鼠新生儿背根神经节 (DRG) 神经元
Catalog #: R-DRG-505
Cryopreserved ampule of Rat Neonatal DRG Neurons containing ≥ 200,000 cells
Product Overview
即用型 CloneticsTM 大鼠背根神经节神经元是通过标准化方法制备的高质量感觉神经元的悬浮液，可立即进行培养。
每瓶背根神经节细胞在 0.25 ml 悬浮液中含有大约 200,000 个细胞。电镀后最初几天会发生细胞死亡，并观察到碎片。这很正常。培养约 4 天后，细胞将形成神经突网络，到第 7 天，碎片将最少。
在没有有丝分裂抑制剂的情况下，神经元倾向于聚集（神经节化）并从底物上脱离。神经节是形成神经中枢的一组神经细胞，尤其是位于大脑或脊髓外的神经细胞。
背根神经节，也称为脊神经节，是每个脊神经节段神经后根的神经节，包含单极初级感觉神经元的细胞体。背根神经节细胞是假单极细胞。假单极细胞有 2 个轴突，而不是一个轴突和树突。一个轴突向脊髓中央延伸；另一个轴突向皮肤或肌肉延伸。
推荐介质：PNGMTM BulletKitTM(CC-4461)
Benefits
- Ready-To-Use
- No tissue dissection or animal handling required
- Increased data reproducibility
- Characteristic morphology and staining tests confirm tissue origin and cell type
- Negative for mycoplasma, bacteria, yeast and fungi
- Certificate of Analysis (CoA) is available for every batch
Applications
- Neurogenesis
- Neurotransmitter Function
- Gene Expression
- Signaling Pathways
- Electrophysiology
- Neurotoxicity
- Drug/Compound Screening
- Advanced Cell Culture Models
Content & Storage
Content
≥200,000 cryopreserved cells per ampule
Related Products
PNGM™ Primary Neuron Growth Medium BulletKit™ CC-4461
PNGM™ 原代神经元生长培养基 BulletKit™ CC-4461
Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons R-EDRG-515
大鼠胚胎背根神经节 (eDRG) 神经元 R-EDRG-515
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012
P3 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-3012
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032
P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010
lonza,lonza PT-8002,PT-5022,PGM-2TM 前脂肪细胞生长培养基-2 BulletKitTM
Catalog #: PT-8002
Culture system containing PGMTM-2 Basal Medium (PT-8202) and PGMTM-2 SingleQuotsTM supplements (PT-9502) required for growth of Preadipocytes
包含前脂肪细胞生长所需的 PGMTM-2 基础培养基 (PT-8202) 和 PGMTM-2 SingleQuotsTM 补充剂 (PT-9502) 的培养系统
Product Overview
PGMTM -2 Predipocyte Growth Medium-2 BulletKitTM 提供增殖培养基和分化培养基，保证诱导分化为成熟的、功能活跃的脂肪细胞。
每瓶 PGM-2TM BulletKitTM 培养基都含有足够的分化培养基，可将 1000 万个前脂肪细胞 [10 个完整的 96 孔板] 完全分化为成熟的功能活跃的脂肪细胞。
PGMTM-2 培养基以 BulletKitTM 培养基（目录号 PT-8002）的形式提供，其中包括基础培养基和前脂肪细胞增殖和分化所需的补充剂。
基础培养基（PGMTM-2 基础培养基；目录号 PT-8202）和必要的补充剂（PGMTM-2 SingleQuotTM 试剂盒；目录号 PT-9502）也可以单独购买。
Content & Storage
Content
1 x PGMTM-2 Basal Medium (PT-8202)
1 x PGMTM-2 SingleQuotsTM Supplement and Growth Factor Pack (PT-9502)
Related Products
Human Subcutaneous Preadipocyte Cells PT-5020
HprAD – Human Subcutaneous Preadipocyte Cells PT-5001
PBM-2 Preadipocyte Basal Medium-2 PT-8202
PBM-2 前脂肪细胞基础培养基-2 PT-8202
PGM-2 Preadipocyte Growth Medium SingleQuots™- Supplements and Growth Factors PT-9502
PGM-2 前脂肪细胞生长培养基 SingleQuots™- 补充剂和生长因子 PT-9502
Trypsin/EDTA for Mesenchymal Stem Cells CC-3232
AdipoRed™ Assay Reagent, 20 mL PT-7009
hMSC – Human Mesenchymal Stem Cells PT-2501
hADSC Human Adipose-Derived Stem Cells PT-5006
DPSC – Human Dental Pulp Stem Cells PT-5025
Diseased Human Visceral Preadipocyte Cells – Diabetes Type I PT-5023
患病的人类内脏前脂肪细胞 – I 型糖尿病 PT-5023
Diseased Human Visceral Preadipocyte Cells – Diabetes Type II PT-5024
患病的人类内脏前脂肪细胞 – II 型糖尿病 PT-5024
Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type I PT-5021
Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type II PT-5022
Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5007
糖尿病 I 型和 II 型脂肪干细胞 (ADSC) PT-5007
Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5008
糖尿病 I 型和 II 型脂肪干细胞 (ADSC) PT-5008
MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ PT-3001
MSCGM™ 间充质干细胞生长培养基 BulletKit™ PT-3001
lonza,PT-5020,人皮下前脂肪细胞,HprAD-人皮下前脂肪细胞,PT-5001
Catalog #: PT-5020
Cryopreserved ampule of Human Subcutaneous Preadipocytes containing ≥1 million cells
Product Overview
PoieticsTM 正常人前脂肪细胞 (HprAD) 是从正常（非糖尿病）供体的皮下或内脏脂肪中分离出来的。 来自糖尿病供体的前脂肪细胞可作为单独的产品获得。 相关的额外捐赠者信息，包括体重指数 (BMI) 可通过联系科学支持获得。
冷冻保存的 PoieticsTM 正常人前脂肪细胞 (HprAD) 保证每安瓿含有≥100 万或≥400 万（仅皮下）细胞，并且脂肪分化检测呈阳性。 所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。
Recommended Media and Reagents
- PGMTM -2 Preadipocyte Growth Medium-2 BulletKitTM Medium (provides both a proliferation medium and a differentiation medium guaranteed to induce differentiation into mature, functionally active adipocytes; Each bottle of the PGM-2TM BulletKitTM Medium contains enough differentiation medium to fully differentiate 10 million preadipocytes [10 complete 96-well plates] into mature, functionally active adipocytes).
PGMTM-2 培养基以 BulletKitTM 培养基（目录号 PT-8002）的形式提供，其中包括基础培养基和前脂肪细胞增殖和分化所需的补充剂。 基础培养基（PGMTM-2 基础培养基；目录号 PT-8202）和必要的补充剂（PGMTM-2 SingleQuotTM 试剂盒；目录号 PT-9502）也可以单独购买。
只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。
Benefits
- Test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- A Certificate ofAnalysis is provided for each cell lot purchased
Content & Storage
Content
1 x Cryopreserved ampule of Preadipocytes containing ≥1 million cells
Related Products
HprAD – Human Subcutaneous Preadipocyte Cells PT-5001
HprAD – 人皮下前脂肪细胞 PT-5001
AdipoRed™ Assay Reagent, 20 mL PT-7009
Trypsin/EDTA for Mesenchymal Stem Cells CC-3232
PBM-2 Preadipocyte Basal Medium-2 PT-8202
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
hMSC – Human Mesenchymal Stem Cells PT-2501
hMSC – 人间充质干细胞 PT-2501
Human Visceral Preadipocyte Cells PT-5005
hADSC Human Adipose-Derived Stem Cells PT-5006
Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5007
Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5008
Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type I PT-5021
Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type II PT-5022
Diseased Human Visceral Preadipocyte Cells – Diabetes Type II PT-5024
患病的人类内脏前脂肪细胞 – II 型糖尿病 PT-5024
PGM-2 Preadipocyte Growth Medium SingleQuots™- Supplements and Growth Factors PT-9502
PGM-2 前脂肪细胞生长培养基 SingleQuots™- 补充剂和生长因子 PT-9502
Diseased Human Visceral Preadipocyte Cells – Diabetes Type I PT-5023
患病的人类内脏前脂肪细胞 – I 型糖尿病 PT-5023
lonza,CC-2540B,支气管上皮细胞培养试剂盒,CC-3171,CC-4175
Catalog #: CC-2540B
完整的细胞培养试剂盒包含一个冷冻保存的支气管上皮细胞安瓿 (CC-2540) 和 BEGMTM 生长培养基 BulletKitTM (CC-3170)，用于培养支气管上皮细胞。
Product Overview
购买我们的支气管上皮细胞培养试剂盒并获得细胞和培养基的折扣价。
该试剂盒由冷冻保存的支气管上皮细胞安瓿（货号 CC-2540）和用于培养这些支气管上皮细胞的 BEGMTM BulletKitTM 生长培养基（货号 CC-3170）组成
Content & Storage
Content
1 x Cryopreserved ampule of Bronchial Epithelial Cells (CC-2540), ≥ 500,000 cells
1 x BEBMTM Bronchial Epithelial Cell Growth Basal Medium (CC-3171), 500 mL
1 x BEGMTM SingleQuotsTM Supplement Pack (CC-4175) containing:
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Amber Vial with Retinoic Acid, 0.50 mL
1 x Natural Cap Vial with Transferrin, 0.50 mL
1 x Amber Vial with Triiodothyronine, 0.50 mL
1 x Amber Vial with Epinephrine, 0.50 mL
1 x Green Cap Vial with hEGF, 0.50 mL
Related Products
BEGMTM Bronchial Epithelial SingleQuotsTM Kit CC-4175
BEGMTM支气管上皮SingleQuotsTM试剂盒CC-4175
BEBM™ Bronchial Epithelial Cell Growth Basal Medium CC-3171
BEBM™ 支气管上皮细胞生长基础培养基 CC-3171
lonza,lonza CC-3249,MGM™-4 黑色素细胞生长培养基-4 BulletKit™
Catalog #: CC-3249
含有黑色素细胞生长所需的 MBMTM-4 基础培养基 (CC-3250) 和 MGMTM-4 SingleQuotsTM 补充剂 (CC-4435) 的培养系统
Product Overview
MGMTM-4 黑色素细胞生长培养基-4 是推荐用于黑色素细胞在培养中最佳生长和存活的培养基。 该介质以我们传统的 BulletKitTM 格式提供，可根据研究需要灵活地添加或省略组件。
ET-3 生长补充剂是成人黑色素细胞生长所需的额外生长因子，单独出售，以补充 MGMTM-4 BulletKitTM 的完整配方，专门设计用于生长成人黑色素细胞。 除了限制培养中的其他细胞类型污染外，ET-3 还提供增强的生长和铺板优势。
Content & Storage
Content
1 x MBMTM-4 Basal Medium (CC-3250), 500 mL
1 x MGMTM-4 SingleQuotsTM Supplement Pack (CC-4435) containing:
1 x Natural Cap Vial with CaCl2, 1.00 mL
1 x Amber Vial with PMA, 0.50 mL
1 x Gray Cap Vial with hFGF-B, 1.00 mL
1 x Lilac Cap Vial with Insulin, 1.00 mL
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Bottle FBS, 2.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
Related Products
MBMTM-4 Melanocyte Growth Basal Medium-4 CC-3250
MBMTM-4 黑色素细胞生长基础培养基-4 CC-3250
MGM™- 4 Melanocyte Growth Medium-4 SingleQuots™ Supplements and Growth Factors CC-4435
MGM™- 4 黑色素细胞生长培养基-4 SingleQuots™ 补充剂和生长因子 CC-4435
lonza,lonza HUCS10P,DonorPlexTM 混合供体人肝细胞，10 个供体，混合性别
Catalog #: HUCS10P
汇集 10 个供体、混合性别的人类肝细胞，具有多种 CYP450、UGT、SULT 和转运蛋白活性。 药物代谢物鉴定、表征和周转动力学的理想选择。
Product Overview
对于 DMPK 研究而言，一致且可靠的汇集供体人肝细胞来源非常重要。 通过我们新的专有算法，我们能够预测 DonorPlexTM 人肝细胞的产量、活力和功能表型，这反过来将支持您的 DMPK 中更一致的结果。 每批汇集的 10 位供体、混合性别的人肝细胞的特征在于活产率，以及 CYP1A2、CYP2A6、CYP2B6、CYP2C9、CYP2C19、CYP2E1、CYP3A4、UGT、SULT、OAT1B1/3、OCT 和 NTCP 活性。
凭借我们大量的单供体人类肝细胞库存，并使用我们的专有算法来预测表型，我们还可以定制生产具有表型的混合供体肝细胞批次，以满足您的需求。
DonorPlex™ 肝细胞 – 悬浮合格肝细胞非常适合短期（<4 小时）体外药物清除试验。 悬浮合格的肝细胞不适用于需要在粘附的单层中培养肝细胞的应用。
Benefits
- Targeted pooling method for predictable metabolism
- Precharacterized for 9 CYPs and 4 drug transporters
- High viability and yields
- Lot sizes < 2000 are possible
Applications
Suspension metabolism studies
Content & Storage
Content
Cryo Vial, >5 million viable cells
Related Products
Human Hepatocytes, Cryopreserved, Plateable HUCPG
人肝细胞，冷冻保存，可电镀 HUCPG
Human Hepatocytes, Cryopreserved Single Donor , Suspension HUCSD
人肝细胞，冷冻保存的单一供体，悬浮 HUCSD
lonza,lonza CC-2516,HMVEC-dNeo-人真皮微血管内皮细胞，新生儿，混合
Catalog #: CC-2516
HMVEC-d Neo, Dermal Microvascular Endothelial, Neonatal, Pooled
Product Overview
真皮微血管内皮细胞包括从成人或新生儿包皮的皮肤组织内的小血管中分离出来的细胞。培养的细胞纯度≥90%，并专门富集真皮 LEC 和 BEC。来自新生儿或成人的真皮微血管细胞 (HMVEC-D) 是血液 (HMVEC-DB1) 和淋巴 (HMVEC-DLy) 内皮细胞的混合群体。
成体细胞用“广告”表示；新生细胞用“Neo”表示。汇集和单一捐助者都可用。通过联系我们的科学支持团队，可以获得更多的捐赠者特征。 CloneticsTM 内皮细胞和培养基方便易用，使研究人员能够专注于结果。 CloneticsTM 细胞、培养基和试剂一起经过质量测试，保证作为一个完整的细胞系统提供最佳性能。
冷冻保存的细胞在第三代运输。冷冻保存的真皮微血管内皮细胞保证通过 15 次群体倍增（CC-2543、CC-2505 和 CC-2516）和 12 次群体倍增（CC-2810、CC-2811、CC-2812 和 CC-2813），表达 CD31/105 ，von Williebrand 因子 VIII，对乙酰化低密度脂蛋白摄取呈阳性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。
Content & Storage
Content
1 x Cryopreserved Ampule with ≥500,000 cells/vial
Related Products
HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult CC-2543
HMVEC-dAd – 人真皮微血管内皮细胞 – 成人 CC-2543
NHEM-Neo – Human Epidermal Melanocytes, Neonatal CC-2504
EGMTM -2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM CC-3202
EGMTM -2 MV 微血管内皮细胞生长培养基-2 BulletKitTM CC-3202
P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5012
P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5024
P5 Primary Cell 4D-Nucleofector™ X Kit S V4XP-5032
P5 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-5032
P5 Primary Cell 96-well Nucleofector™ Kit V4SP-5096
P5 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-5960
P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5002
P5 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-5002
P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5010
P5 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-5010
lonza,CC-3207,OGM™ 成骨细胞生长培养基 BulletKit™
Catalog #: CC-3207
OGMTM Osteoblasts BulletKitTM
Product Overview
CloneticsTM OGMTM 成骨细胞生长培养基专为人类成骨细胞的生长和扩增而配制。 该试剂盒含有 10% 的血清。 该试剂盒包装方便，包括基础培养基和生长补充剂，用于成骨细胞的完全培养
OGMTM 分化补充剂也可单独购买和销售（目录号 CC-4194）。 这些推荐用于分化测定。
Benefits
Applications:
- In vitro bone models
- Mineralization
- Osteoporosis
- Gene expression
- Hormone treatments
- Osteopenia
- Paget’s Disease
Content & Storage
Content
1 x Bottle Basal Medium 500 mL
1 x Bottle FBS 50.00 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Blue Cap Vial with Ascorbic Acid 0.50 mL
Related Products
NHOst – Human Osteoblasts CC-2538
NHOst – 人类成骨细胞 CC-2538
OBM™ Osteoblast Growth and Differentiation Basal Medium CC-3208
OBM™ 成骨细胞生长和分化基础培养基 CC-3208
OGM™ Osteoblast Growth Medium SingleQuots™ Supplements and Growth Factors CC-4193
OGM™ 成骨细胞生长培养基 SingleQuots™ 补充剂和生长因子 CC-4193
OGM™ Osteoblast Differentiation Medium SingleQuots™ Supplements and Growth Factors CC-4194
OGM™ 成骨细胞分化培养基 SingleQuots™ 补充剂和生长因子 CC-4194
lonza,原代细胞和干细胞,FGM™ 成纤维细胞生长培养基 BulletKit™
Catalog #: CC-3130
停产通知：该产品即将停产。 当前库存将被出售，直到库存耗尽。 请使用产品 CC-3132 作为替代品。
用于人成纤维细胞培养的成纤维细胞生长培养基 BulletKitTM
Product Overview
FGMTM Fibroblast Growth Medium was developed to support growth of fibroblasts.
Content & Storage
Content
1 x FBMTM Basal Medium (CC-3131), 500 mL
1 x FGM SingleQuotsTM Supplement Pack (CC-4134) containing:
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Gray Cap Vial with hFGF-B, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
lonza,lonza HUCNP,lonza HUCLS,原代细胞和干细胞,人肝混合非实质细胞
Catalog #: HUCNP
Human cryopreserved liver mixed NPCs cells
人冷冻保存的肝脏混合 NPCs 细胞
Product Overview
Human Cryopreserve Liver Mixed NPCs are a native mixture of Non-parenchymal cells from human liver. These are supplied in > 1million cells/vial and can have a varying amount of the different non-parenchymal cells.
This product is most suitable for liver cell co-culture applications
人冷冻保存肝脏混合 NPC 是来自人肝脏的非实质细胞的天然混合物。 这些以 > 100 万个细胞/小瓶的形式提供，并且可以具有不同数量的不同非实质细胞。
本产品最适合肝细胞共培养应用
Benefits
- Native cell ratios,
- Never passaged for close to in vivo function
Applications
ADMETox, Liver Disease, NASH
Content & Storage
Content
Cryo Vial
Related Products
Stellate cells, human cryopreserved, passage 1 HUCLS
星状细胞，人类冷冻保存，第 1 代 HUCLS
lonza,lonza 2M-125C,原代细胞和干细胞,人骨髓单个核细胞 MNC
货号: 2M-125C
Cryopreserved ampule of Human Bone Marrow Mononuclear Cells containing ≥25 million cells
含有≥2500万个细胞的人骨髓单个核细胞的冷冻保存安瓿
Product Overview
Bone marrow mononuclear cells (common abbreviations: BMNC, BM MNC, and BM-MNC) are a mixed population of single nucleus cells including monocytes, lymphocytes, and hematopoietic stem and progenitor cells.
骨髓单个核细胞（常用缩写：BMNC、BM MNC 和 BM-MNC）是单核细胞的混合群体，包括单核细胞、淋巴细胞以及造血干细胞和祖细胞。
BMNCs You Can Count on to Perform
Cryopreserved Lonza Bone Marrow Mononuclear Cells are isolated from whole bone marrow via density gradient separation. Isolating mononuclear cells eliminates erythrocytes and graulocytes, producing a more stable cell product. Lonza BMNCs are available fresh or frozen from a large and varied donor pool. They are guaranteed to contain either ≥5, 25, 100, 200 and 300 million viable cells per ampoule, depending upon product purchased. Fresh cells are suspended in HBSS with 5 mM EDTA and 0.5% BSA and are available in a variety of cells per vial packaging sizes. Fresh BMNC orders require a minimum order quantity of two vials per donor. Please contact Customer Service for information on sizes and pricing for fresh cells.
通过密度梯度分离从全骨髓中分离冷冻保存的龙沙骨髓单核细胞。 分离单核细胞消除红细胞和粒细胞，产生更稳定的细胞产物。 Lonza BMNCs 可从大量多样的供体库中获得新鲜或冷冻。 根据购买的产品，它们保证每个安瓿含有≥5、25、100、200 和 3 亿个活细胞。 新鲜细胞悬浮在含有 5 mM EDTA 和 0.5% BSA 的 HBSS 中，每瓶包装尺寸有多种细胞可供选择。 新鲜的 BMNC 订单要求每个捐赠者至少订购两瓶。 有关新鲜细胞的尺寸和价格信息，请联系客户服务。
Choices in Cell Type and Tissue Source
Lonza offers choices in cell type and tissue source so you can choose the right product for your research and budget needs. Depending upon your ultimate cell of choice, you can choose from unprocessed bone marrow, bone marrow MNCs, bone marrow CD34+ cells, bone marrow stromal cells (for use as feeder cells), or bone marrow mesenchymal stem cells. In addition, if bone marrow is not your preferred tissue source, we also offer cord blood derived cells.
Lonza 提供细胞类型和组织来源的选择，因此您可以选择适合您的研究和预算需求的产品。 根据您选择的最终细胞，您可以选择未加工的骨髓、骨髓 MNC、骨髓 CD34+ 细胞、骨髓基质细胞（用作饲养细胞）或骨髓间充质干细胞。 此外，如果骨髓不是您首选的组织来源，我们还提供脐带血来源的细胞。
Supported by an Optimized Culture System
HPGMTM Hematopoietic Growth Medium is recommended for optimal performance in serum-free culturing (additional cytokines may be necessary depending on application). Alternative media containing serum may also be acceptable. Please contact Scientific Support for serum containing media recommendations.
Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.
建议使用 HPGMTM 造血生长培养基以在无血清培养中获得最佳性能（根据应用，可能需要额外的细胞因子）。 含有血清的替代培养基也是可以接受的。 有关含血清培养基的建议，请联系科学支持。
只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。 如果您在选择合适的培养基、试剂或方案方面需要帮助，请联系 Lonza Scientific Support。
Benefits
- Test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- A Certificate ofAnalysis is provided for each cell lot purchased
- 支原体、细菌、酵母菌和真菌检测呈阴性
并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎
为购买的每个细胞批次提供分析证书
Content & Storage
Content
1 x Cryopreserved ampule BMNCs containing ≥ 25 million cells
Instructions, SDS, CoA
Safety Data Sheets (SDS)
View SDS
SDS – Dutch
SDS – German
SDS – Italian
SDS – Spanish
SDS – French
SDS – English
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Unprocessed Human Bone Marrow, Fresh, 10 mL 1M-105
未加工的人骨髓，新鲜，10 mL 1M-105
Human Bone Marrow Stromal Cells, 5 Million 2M-302
人骨髓基质细胞，500 万个 2M-302
Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702
人外周血单个核细胞 (hPBMC)，冷冻保存，5000 万个 CC-2702
hMSC – Human Mesenchymal Stem Cells PT-2501
hMSC – 人间充质干细胞 PT-2501
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218
lonza,lonza 00195769,KGM™ Gold 角质形成细胞生长培养基 BulletKit™
Catalog #: 00195769
KGMTM Gold Keratinocyte BulletKitTM, Calcium Free
KGMTM Gold Keratinocyte BulletKitTM，无钙
Product Overview
A new robust and versatile serum-free medium formulation for high and low density NHEK-Normal Human Epidermal Keratinocyte cultures.
KGM-GoldTM Calcium Free Keratinocyte Growth Medium is designed to support both clonal growth and high density keratinocyte proliferation. The medium supports superior growth and morphology of keratinocytes.
Furthermore, quality control at Lonza requires that keratinocytes pass strict morphological criteria specifying the minimal presence of enlarged and vacuolated cells, enabling Lonza keratinocytes to be used by the researcher for ≥15 Population Doublings.
一种新的稳健且多功能的无血清培养基配方，适用于高密度和低密度 NHEK-Normal 人类表皮角质形成细胞培养物。
KGM-GoldTM 无钙角质形成细胞生长培养基旨在支持克隆生长和高密度角质形成细胞增殖。 该培养基支持角质形成细胞的卓越生长和形态。
此外，Lonza 的质量控制要求角质形成细胞通过严格的形态学标准，规定最小存在扩大和空泡细胞，使研究人员能够将 Lonza 角质形成细胞用于≥15 人口倍增。
Content & Storage
Content
1 x Bottle Basal Medium 500 mL
1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Natural Cap Vial with Transferrin 0.50 mL
1 x Amber Vial with Epinephrine 0.25 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Orange Cap Vial with BPE 2.00 mL
1 x Green Cap Vial with hEGF 0.50 mL
1 x Lilac Cap Vial with Insulin 0.50 mL
Instructions, SDS, CoA
Safety Data Sheets (SDS)
View SDS
SDS – German
SDS – Italian
SDS – Dutch
SDS – French
SDS – Spanish
SDS – English
Related Products
KBM™ Gold Keratinocyte Growth Basal Medium, Calcium and Phenol Red Free 00195130
KBM™ Gold 角质形成细胞生长基础培养基，不含钙和酚红 00195130
KGM™ Gold Keratinocyte Growth Medium SingleQuots™ Supplements and Growth Factors 00192152
KGM™ Gold 角质形成细胞生长培养基 SingleQuots™ 补充剂和生长因子 00192152
Data
Morphology of Lonza’s Adult Keratinocytes Grown in KGM™ Gold Growth Medium Compared to Competitor A’s Adult Keratinocytes in Their Medium.
The growth performance and longevity of Competitor A’s adult keratinocytes grown in Competitor A’s Medium were compared with Lonza’s adult keratinocytes grown in KGM-Gold™ Growth Medium. Although Competitor A claims ≥25 total population doublings for their adult keratinocytes, actual results show that this lot barely obtained 19 PD. Note the presence of several enlarged cells in Passage 2 in Competitor’s Medium. At the end of passage 4 (15 PD) Lonza’s adult keratinocytes consist of mostly small proliferative cells.
将竞争对手 A 的成年角质形成细胞在竞争对手 A 的培养基中生长的生长性能和寿命与龙沙在 KGM-Gold™ 生长培养基中生长的成年角质形成细胞进行了比较。 尽管竞争对手 A 声称其成年角质形成细胞的总种群倍增 ≥ 25，但实际结果表明，该批次几乎没有获得 19 个 PD。 注意竞争对手培养基中第 2 段中存在几个扩大的细胞。 在第 4 代（15 PD）结束时，Lonza 的成人角质形成细胞主要由小的增殖细胞组成。