标签：LI-COR VRDye™ 490 蛋白质标记试剂盒

These kits may be used to label proteins spanning the molecular weight range of 50 kDa to 200 kDa. Labeled antibodies can be used for applications such as flow cytometry, microscopy, and immunohistochemistry.

这些试剂盒可用于标记分子量范围为 50 kDa 至 200 kDa 的蛋白质。 标记的抗体可用于流式细胞术、显微镜检查和免疫组织化学等应用。

Absorbance and Emission Spectra in 1X PBS

High MW Kit

This kit is optimized for labeling 1 mg of a primary or secondary IgG antibody.

This kit contains:

• 3 x 0.15 mg of VRDye 490 Reactive Dye (store at -20 °C)
• 0.5 mL 1 M Potassium Phosphate (K₂HPO₄), pH 9 (store at 4 °C)
• 25 mL PBS (store at 4 °C)
• 0.5 mL Ultra Pure Water (store at 4 °C)
• 3 x Pierce^® Zeba™ Desalting Spin Columns (store at 4 °C)
  The minimum recommended protein molecular weight
  for these columns is 7 kDa.
• Pierce Zeba Desalting Spin Column instructions
• Protocol for VRDye Protein Labeling Kit – High MW
• Quick Reference Guide

High MW-Microscale Kit

This kit is optimized for labeling 100 µg of a primary or secondary IgG antibody.

该试剂盒针对标记 100 µg 一抗或二抗 IgG 进行了优化。

This kit contains:

• 3 x 0.15 mg of VRDye 490 Reactive Dye (store at -20 °C)
• 0.5 mL 1 M Potassium Phosphate (K₂HPO₄), pH 9 (store at 4 °C)
• 25 mL PBS (store at 4 °C)
• 0.5 mL Ultra Pure Water (store at 4 °C)
• 3 x Pierce Zeba Desalting Spin Columns (store at 4 °C)
  The minimum recommended protein molecular weight
  for these columns is 7 kDa.
• Pierce Zeba Desalting Spin Column instructions
• Protocol for VRDye Protein Labeling Kit – High MW-Microscale
• Quick Reference Guide