Reagents > VRDye™ 490 Goat anti-Rabbit IgG Secondary Antibody,VRDye™ 490 山羊抗兔 IgG 二抗
Immunogen
Rabbit IgG
Purity and Specificity
Isolation of specific antibodies was accomplished by affinity chromatography using pooled rabbit IgG covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA. This antibody was tested by dot blot and and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for immunofluorescence microscopy and flow cytometry applications.
使用与琼脂糖共价连接的混合兔 IgG，通过亲和层析实现特异性抗体的分离。 基于 ELISA 和流式细胞术，该抗体与兔 IgG 的重链和轻链以及兔 IgM 和 IgA 的轻链反应。 通过斑点印迹和/或固相吸附测试该抗体与人、小鼠、大鼠、绵羊和鸡血清蛋白的交叉反应最小，但可能与来自其他物种的免疫球蛋白交叉反应。 该偶联物经过专门测试，可用于免疫荧光显微镜和流式细胞术应用。
Applications
- Microscopy
- Immunohistochemistry
- Flow Cytometry
Formulation
VRDye 490 secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
VRDye 490 二抗以纯化的免疫球蛋白偶联物形式提供，在磷酸盐缓冲盐水中冻干，pH 7.4。 避光。 在重组前储存在 4°C。
复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。
Recommended Dilutions
| Application | Recommended | Suggested Range |
|---|---|---|
| Immunofluorescence Microscopy | 1:400 | 1:100 – 1:1,000 |
| Flow Cytometry | 1:1,000 | 1:200 – 1:2,000 |
| Other | User-optimized |
Suggested working dilutions are given as a guide only. Optimum dilutions will vary and should be determined empirically.
Example Data
