标签：LI-COR VRDye 490 二抗

Reagents > VRDye™ 490 Goat anti-Mouse IgG Secondary Antibody,VRDye™ 490 山羊抗小鼠 IgG 二抗

Immunogen

Mouse IgG paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG₁, IgG_2a, IgG_2b, and IgG₃, and with the light chains of mouse IgM and IgA. This antibody was tested by dot blot and and/or solid-phase adsorbed for minimal cross-reactivity with human, rabbit, goat, rat, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for immunofluorescence microscopy and flow cytometry applications.

使用与琼脂糖共价连接的混合小鼠 IgG 通过亲和层析实现特异性抗体的分离。 基于 ELISA 和流式细胞术，该抗体与小鼠 IgG1、IgG2a、IgG2b 和 IgG3 的重链和轻链以及小鼠 IgM 和 IgA 的轻链反应。 通过斑点印迹和/或固相吸附测试该抗体与人、兔、山羊、大鼠和马血清蛋白的交叉反应最小，但可能与来自其他物种的免疫球蛋白交叉反应。 该偶联物经过专门测试，可用于免疫荧光显微镜和流式细胞术应用。

Applications

• Microscopy
• Immunohistochemistry
• Flow Cytometry

Formulation

VRDye 490 secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

VRDye 490 二抗以纯化的免疫球蛋白偶联物形式提供，在磷酸盐缓冲盐水中冻干，pH 7.4。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application	Recommended	Suggested Range
Immunofluorescence Microscopy	1:400	1:100 – 1:1,000
Flow Cytometry	1:1,000	1:200 – 1:2,000
Other	User-optimized

Suggested working dilutions are given as a guide only. Optimum dilutions will vary and should be determined empirically.

Reagents > VRDye™ 490 Donkey anti-Goat IgG Secondary Antibody,VRDye™ 490 驴抗山羊 IgG 二抗

Immunogen

Goat IgG

Purity and Specificity

The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on ELISA, this antibody reacts with heavy and light chains of goat IgG and sheep IgG. This antibody was tested by dot blot and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, rabbit, rat, chicken, guinea pig, hamster, swine, and horse serum proteins but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

使用与琼脂糖珠偶联的抗原，通过免疫亲和层析从抗血清中分离抗体。 基于 ELISA，该抗体与山羊 IgG 和绵羊 IgG 的重链和轻链反应。 该抗体通过斑点印迹和/或固相吸附测试，与人、小鼠、兔、大鼠、鸡、豚鼠、仓鼠、猪和马血清蛋白的交叉反应最小，但可能与来自其他物种的免疫球蛋白交叉反应 . 该偶联物经过专门测试，可用于蛋白质印迹应用。

Applications

• Western blotting

Formulation

VRDye 490 secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C before and after reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

VRDye 490 二抗以纯化的免疫球蛋白偶联物形式提供，在磷酸盐缓冲盐水中冻干，pH 7.4。 避光。 在重组之前和之后储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application	Recommended	Suggested Range
Western Blot Detection	1:10,000	1:5,000 – 1:15,000
Other	User-optimized

Suggested working dilutions are given as a guide only. Optimum dilutions will vary and should be determined empirically.

Reagents > VRDye™ 490 Donkey anti-Mouse IgG Secondary Antibody,VRDye™ 490 驴抗小鼠 IgG 二抗

Immunogen

Mouse IgG

Purity and Specificity

The antibody was isolated by affinity chromatography using antigens coupled to agarose beads. Based on ELISA, this antibody reacts with heavy and light chains of mouse IgG and the light chains of mouse IgM and IgA. This antibody was tested by ELISA and/or solid phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, horse, human, rabbit, and sheep serum proteins but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

使用与琼脂糖珠偶联的抗原通过亲和层析分离抗体。 基于 ELISA，该抗体与小鼠 IgG 的重链和轻链以及小鼠 IgM 和 IgA 的轻链反应。 该抗体通过 ELISA 和/或固相吸附测试，以确保与牛、鸡、山羊、豚鼠、马、人、兔和绵羊血清蛋白的交叉反应最小，但可能与来自其他物种的免疫球蛋白交叉反应。 该偶联物经过专门测试，可用于蛋白质印迹应用。

Applications

• Western blotting

Formulation

VRDye 490 secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C before and after reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

VRDye 490 二抗以纯化的免疫球蛋白偶联物形式提供，在磷酸盐缓冲盐水中冻干，pH 7.4。 避光。 在重组之前和之后储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application	Recommended	Suggested Range
Western Blot Detection	1:10,000	1:5,000 – 1:15,000
Other	User-optimized

Suggested working dilutions are given as a guide only. Optimum dilutions will vary and should be determined empirically.

Reagents > VRDye™ 490 Donkey anti-Rabbit IgG Secondary Antibody,VRDye™ 490 驴抗兔 IgG 二抗

Immunogen

Rabbit IgG

Purity and Specificity

The antibody was isolated by affinity chromatography using antigens coupled to agarose beads. Based on ELISA, this antibody reacts with heavy and light chains of rabbit IgG and light chains common to most rabbit immunoglobulins. This antibody has been tested by ELISA and/or solid phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

使用与琼脂糖珠偶联的抗原通过亲和层析分离抗体。 基于 ELISA，该抗体与兔 IgG 的重链和轻链以及大多数兔免疫球蛋白常见的轻链发生反应。 该抗体已通过 ELISA 和/或固相吸附测试，以确保与牛、鸡、山羊、豚鼠、仓鼠、马、人、小鼠、大鼠和绵羊血清蛋白的交叉反应最小，但可能与免疫球蛋白发生交叉反应 来自其他物种。 该偶联物经过专门测试，可用于蛋白质印迹应用。

Applications

• Western blotting

Formulation

VRDye 490 secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C before and after reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

VRDye 490 二抗以纯化的免疫球蛋白偶联物形式提供，在磷酸盐缓冲盐水中冻干，pH 7.4。 避光。 在重组之前和之后储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application	Recommended	Suggested Range
Western Blot Detection	1:10,000	1:5,000 – 1:15,000
Other	User-optimized

Suggested working dilutions are given as a guide only. Optimum dilutions will vary and should be determined empirically.