Reagents > IRDye® 800RS NHS Ester,IRDye® 800RS NHS 酯
IRDye 800RS infrared dye is an excellent choice for nucleic acid applications. This near-infrared fluorescence dye has good water solubility, but low salt tolerance. It is more hydrophobic than IRDye 800CW.
IRDye 800RS NHS ester can be used to label the primary and secondary amino groups of DNA and RNA. Nucleic acids that have been labeled with IRDye 800RS can be easily purified by reverse-phase chromatography.
IRDye 800RS 红外染料是核酸应用的绝佳选择。 这种近红外荧光染料具有良好的水溶性，但耐盐性低。 它比 IRDye 800CW 更疏水。
IRDye 800RS NHS 酯可用于标记 DNA 和 RNA 的伯氨基和仲氨基。 用 IRDye 800RS 标记的核酸可以很容易地通过反相色谱法纯化。
IRDye Infrared Dyes Overview
Dye Structure
Properties
- Chemical Formula: C50H56N3NaO11S2
- Molecular Weight: 962.12 g/mol
- Exact Mass: 961.33
Absorption and Emission Spectra
| Solvent | Ext. Coeff. (M-1cm-1) | Absorption Maxima | Emission Maxima | Stokes Shift |
|---|---|---|---|---|
| Methanol | 300,000 | 770 nm | 786 nm | 16 nm |
| Water | 200,000 | 767 nm | 786 nm | 19 nm |
| 1X PBS | 200,000 | 767 nm | 786 nm | 19 nm |
| PBS: Methanol | 270,000 | 770 nm | 786 nm | 16 nm |
Absorption and Emission Spectra in 1X PBS
