标签：LI-COR IRDye® 800CW EGF 光学探头

LI-COR IRDye® 800CW EGF 光学探头,LI-COR IRDye® 800CW EGF

Reagents > IRDye® 800CW EGF Optical Probe,IRDye® 800CW EGF 光学探头

IRDye® 800CW EGF Optical Probe

IRDye 800CW EGF Optical Probe is a near-infrared (NIR) fluorescently-labeled recombinant human epidermal growth factor (EGF). This optical imaging agent is composed of a recombinant EGF polypeptide containing 54 amino acid residues (molecular weight = 6.2 kDa) conjugated to the LI-COR IRDye 800CW infrared dye.

IRDye 800CW EGF provides a versatile probe that can be used for in vitro and in vivo assays, as well as whole organ and tissue section analysis. In vivo assays of EGF binding have proven effective in monitoring specific solid tumor models that exhibit overexpression of EGFR.

Epidermal growth factor receptor (EGFR) is one of a family of receptor tyrosine kinases found on the surface of epithelial cells, to which EGF binds. Many types of cancer cells have abnormally high EGFR levels on the cell surface (table below).

IRDye 800CW EGF 光学探针是一种近红外 (NIR) 荧光标记的重组人表皮生长因子 (EGF)。 这种光学显像剂由与 LI-COR IRDye 800CW 红外染料偶联的含有 54 个氨基酸残基（分子量 = 6.2 kDa）的重组 EGF 多肽组成。

IRDye 800CW EGF 提供了一种多功能探针，可用于体外和体内测定，以及整个器官和组织切片分析。 已证明 EGF 结合的体内测定可有效监测表现出 EGFR 过表达的特定实体瘤模型。

表皮生长因子受体 (EGFR) 是在上皮细胞表面发现的受体酪氨酸激酶家族之一，EGF 与之结合。 许多类型的癌细胞在细胞表面具有异常高的 EGFR 水平（下表）。

Tumor Type % of Tumors Over-Expressing EGFR
Head and Neck 80-100
Renal Cell 50-90
Non-small-cell Lung 40-80
Glioma 40-63
Ovarian 35-70
Bladder 31-48
Pancreatic 30-50
Colon 25-77
Breast 14-91

Frequency of elevated EGFR expression in different types of epithelial tumors1.

Learn about other LI-COR optical probes.

Applications

  • In-Cell Western™ Assay
  • Microscopy
  • On-Cell Western Assay
  • RNAi Screens
  • Optical Probe Development
  • Target Specificity
  • Tissue Section Imaging
  • In-Cell Western™ 检测
    显微镜
    On-Cell Western 检测
    RNAi 筛选
    光学探头开发
    目标特异性
    组织切片成像

Absorbance and Emission Spectra

IRDye® 800CW EGF Optical Probe

Determine Binding Specificity with IRDye 800CW EGF

In-Cell Western assays on the Odyssey® CLx, Odyssey Classic, or Odyssey Sa Infrared Imaging Systems were used to determine binding specificity of the probe in vitro for various cell lines.

IRDye® 800CW EGF Optical Probe
Two different cell lines known to over-express the EGFR were characterized by evaluating: a) binding of IRDye 800CW EGF; b) IRDye 800CW only; and c) a competition assay using unlabeled EGF to compete for EGFR sites.

In vivo Data Example

IRDye® 800CW EGF Optical Probe
Intravenous administration of IRDye 680RD EGF (2 nmol) binds to the A431 tumor located on the right hip. Image captured 24 h post injection on the Pearl® Impulse Imaging System.

References

  1. Herbst, R.S., & Shin, DM (2002). Cancer, (94) 5 DOI 10.1002/cncr.10372.10372.
  2. Kovar, J. L., Volcheck, W. M., Chen, J., & Simpson, M. A. (2007). Anal Biochem, 361(1), 47–54. doi: 10.1016/j.ab.2006.11.021.