标签：LI-COR IRDye® 800CW 驴抗鸡二抗

LI-COR IRDye 800CW 二抗,LI-COR IRDye® 800CW 驴抗鸡二抗

Reagents > IRDye® 800CW Donkey anti-Chicken Secondary Antibody,IRDye® 800CW 驴抗鸡二抗

IRDye® 800CW Donkey anti-Chicken Secondary Antibody

Immunogen

Chicken IgY, whole molecule. IgY is the original designation for the IgG-like protein found in both serum and egg yolk.

Purity and Specificity

The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule chicken IgY, and with the light chains of other chicken immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

使用与琼脂糖珠偶联的抗原，通过免疫亲和层析从抗血清中分离抗体。 基于免疫电泳和/或 ELISA，该抗体与全分子鸡 IgY 以及其他鸡免疫球蛋白的轻链反应。 未检测到针对非免疫球蛋白血清蛋白的反应性。 该抗体通过 ELISA 和/或固相吸附测试，以确保与牛、山羊、豚鼠、叙利亚仓鼠、马、人、小鼠、兔、大鼠和绵羊血清蛋白的交叉反应最小，但可能发生交叉反应 与来自其他物种的免疫球蛋白。 该偶联物经过专门测试，可用于蛋白质印迹应用。

Applications

Highly recommended for:

  • Western Blot
  • In-Cell Western™ Assay
  • On-Cell Western Assay
  • Protein Array
  • Immunohistochemistry
  • Small Animal Imaging
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging
  • Virus Titration Assay
  • 蛋白质印迹
    In-Cell Western™ 检测
    On-Cell Western 检测
    蛋白质阵列
    免疫组织化学
    小动物成像
    显微镜
    2D 凝胶检测
    组织切片成像
    病毒滴定法

Formulation

IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 800CW 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:15,000 1:5,000 – 1:25,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

RRID

  • P/N 925-32218: RRID AB_2814922
  • P/N 926-32218: RRID AB_1850023

Example Data

IRDye® 800CW Donkey anti-Chicken Secondary Antibody
Detection of actin with IRDye 800CW Donkey anti-chicken. Odyssey® One-Color Molecular Weight Marker (P/N 928-40000) and C32 lysate, 3 µg – 0.01 µg (Santa Cruz P/N sc-2205) were resolved on a 10% Bis Tris gel and transferred to Odyssey Nitrocellulose membrane (P/N 926-31092). Membrane was blocked with Odyssey Blocking Buffer (P/N 927-40000) followed by detection with chicken anti-actin (GenWay P/N 15-288-23014) and IRDye 800CW Donkey anti-Chicken (P/N 926-32218).
用 IRDye 800CW 驴抗鸡检测肌动蛋白。 Odyssey® One-Color Molecular Weight Marker (P/N 928-40000) 和 C32 裂解物，3 µg – 0.01 µg (Santa Cruz P/N sc-2205) 在 10% Bis Tris 凝胶上分离并转移到 Odyssey 硝酸纤维素膜上 （部件号 926-31092）。 用 Odyssey 封闭缓冲液 (P/N 927-40000) 封闭膜，然后用鸡抗肌动蛋白 (GenWay P/N 15-288-23014) 和 IRDye 800CW Donkey anti-Chicken (P/N 926-32218) 进行检测。