标签：LI-COR IRDye® 800CW 驴抗豚鼠 IgG 二抗

Immunogen

Guinea Pig IgG, whole molecule

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule guinea pig IgG as well as the light chains common to other guinea pig immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications.

使用固定在琼脂糖珠上的抗原通过亲和层析实现特异性抗体的分离。 基于免疫电泳和/或 ELISA，该抗体与全分子豚鼠 IgG 以及其他豚鼠免疫球蛋白共有的轻链反应。 未检测到针对非免疫球蛋白血清蛋白的反应性。 该抗体已通过 ELISA 和/或固相吸附测试，以确保与牛、鸡、山羊、叙利亚仓鼠、马、人、小鼠、兔、大鼠和绵羊血清蛋白的交叉反应最小。 该偶联物经过专门测试，可用于蛋白质印迹和 In-Cell Western™ 检测应用。

Applications

Highly recommended for:

• Western Blot
• In-Cell Western Assay
• On-Cell Western Assay
• Protein Array
• Immunohistochemistry
• Small Animal Imaging
• Microscopy
• 2D Gel Detection
• Tissue Section Imaging
• Virus Titration Assay
• 蛋白质印迹
  细胞内西方检测
  On-Cell Western 检测
  蛋白质阵列
  免疫组织化学
  小动物成像
  显微镜
  2D 凝胶检测
  组织切片成像
  病毒滴定法

Formulation

IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 800CW 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Optimum dilutions will vary and should be determined empirically.

RRID

• P/N 925-32411: RRID AB_2814905
• P/N 926-32411: RRID AB_1850024

Example Data