标签：LI-COR IRDye® 800CW 山羊抗小鼠 IgG2b 特异性二抗

Immunogen

Mouse IgG_2b paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography on mouse IgG_2b covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG_2b. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgM, IgG₁, IgG_2a, IgG₃, and IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Results with your primary antibody may vary and specificity should be confirmed prior to performing two-color detection.

通过在共价连接到琼脂糖的小鼠 IgG2b 上进行亲和层析来实现特异性抗体的分离。 基于 ELISA 和流式细胞术，该抗体与小鼠 IgG2b 的重链发生反应。 该抗体已通过斑点印迹和/或固相吸附测试，以确保与小鼠 IgM、IgG1、IgG2a、IgG3 和 IgA、合并的人血清和纯化的人副蛋白的交叉反应最小。 该偶联物经过专门测试，可用于蛋白质印迹和 In-Cell Western™ 检测应用。 一抗的结果可能会有所不同，在进行双色检测之前应确认特异性。

Applications

Highly recommended for:

• Western Blot
• In-Cell Western Assay
• On-Cell Western Assay
• Protein Array
• Immunohistochemistry
• Small Animal Imaging
• Microscopy
• 2D Gel Detection
• Tissue Section Imaging
• Virus Titration Assay
• 蛋白质印迹
  细胞内西方检测
  On-Cell Western 检测
  蛋白质阵列
  免疫组织化学
  小动物成像
  显微镜
  2D 凝胶检测
  组织切片成像
  病毒滴定法

Formulation

IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 800CW 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Optimum dilutions will vary and should be determined empirically.

RRID

• P/N 926-32352: RRID AB_2782999

Example Data