标签：LI-COR IRDye® 800CW 山羊抗大鼠 IgG 二抗

LI-COR IRDye® 800CW 山羊抗大鼠 IgG 二抗, LI-COR IRDye® 800CW 二抗

Reagents > IRDye® 800CW Goat anti-Rat IgG Secondary Antibody,IRDye® 800CW 山羊抗大鼠 IgG 二抗

IRDye® 800CW Goat anti-Rat IgG Secondary Antibody

Immunogen

Rat IgG, whole molecule

Purity and Specificity

The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule rat IgG, and with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse, bovine, horse, human, and rabbit serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

使用与琼脂糖珠偶联的抗原，通过免疫亲和层析从抗血清中纯化抗体。 基于免疫电泳和/或 ELISA，该抗体与全分子大鼠 IgG 以及其他大鼠免疫球蛋白的轻链发生反应。 未检测到针对非免疫球蛋白血清蛋白的抗体。 该抗体已通过 ELISA 和/或固相吸附测试，以确保与小鼠、牛、马、人类和兔血清蛋白的交叉反应最小，但可能与其他物种的免疫球蛋白交叉反应。 该偶联物经过专门测试，可用于蛋白质印迹应用。

Applications

Highly recommended for:

  • Western Blot
  • In-Cell Western™ Assay
  • On-Cell Western Assay
  • Protein Array
  • Immunohistochemistry
  • Small Animal Imaging
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging
  • Virus Titration Assay

Formulation

IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 800CW 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:15,000 1:5,000 – 1:25,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

RRID

  • P/N 925-32219: RRID AB_2721932
  • P/N 926-32219: RRID AB_1850025

Example Data

IRDye® 800CW Goat anti-Rat IgG Secondary Antibody
Western blot detection of tubulin in C32 lysate. C32 lysate (Santa Cruz P/N sc- 2205) was resolved on a 10% Tris-Glycine gel and transferred to Odyssey® nitrocellulose (P/N 926-31090). The membrane was probed with rat anti-tubulin (Novus Biologicals P/N NB600-506) followed by detection with IRDye 800CW Goat anti-Rat IgG (P/N 926-32219).
蛋白质印迹检测 C32 裂解物中的微管蛋白。 C32 裂解物 (Santa Cruz P/N sc-2205) 在 10% Tris-Glycine 凝胶上分离并转移到 Odyssey® 硝酸纤维素 (P/N 926-31090)。 用大鼠抗微管蛋白（Novus Biologicals P/N NB600-506）探测膜，然后用 IRDye 800CW 山羊抗大鼠 IgG（P/N 926-32219）检测。