标签：LI-COR IRDye 800CW

LI-COR IRDye 800CW ,IRDye® 800CW 炔烃红外染料

Reagents > IRDye® 800CW Alkyne Infrared Dye,IRDye® 800CW 炔烃红外染料

IRDye® 800CW Alkyne Infrared Dye

IRDye 800CW Alkyne provides the functionality for preferential labeling of molecules containing azide groups (–N3) with IRDye 800CW.

IRDye 800CW Alkyne 提供了使用 IRDye 800CW 优先标记含有叠氮基 (–N3) 的分子的功能。

Learn more about Near-Infrared Fluorescent Click Chemistry Reagents.

Dye Structure

IRDye® 800CW Alkyne Infrared Dye

Properties

  • Chemical Formula: C49H54N3Na3O14S4
  • Molecular Weight: 1106.19 g/mol
  • Exact Mass: 1105.22
  • Correction Factor at 260 – 280 nm: 3%

Absorption and Emission Spectra

Solvent Absorption Maxima Emission Maxima
Methanol 778 nm 794 nm
1X PBS 774 nm 789 nm
1:1 Methanol to 1X PBS 778 nm

Absorbance and Emission Spectra in 1X PBS

IRDye® 800CW Alkyne Infrared Dye

Molar Absorptivity

Solvent Molar Absorptivity (L mol-1 cm-1)
Methanol 300,000
1X PBS 240,000
1:1 Methanol to 1X PBS 270,000

LI-COR IRDye® 800CW,IRDye® 800CW 叠氮化物红外染料

Reagents > IRDye® 800CW Azide Infrared Dye,IRDye® 800CW 叠氮化物红外染料

IRDye® 800CW Azide Infrared Dye

IRDye 800CW Azide provides the functionality for preferential labeling of molecules that contain either the alkyne or dibenzocyclooctyne (DBCO) group.

IRDye 800CW 叠氮化物提供优先标记含有炔烃或二苯并环辛炔 (DBCO) 基团的分子的功能。

Learn more about Near-Infrared Fluorescent Click Chemistry Reagents.

Dye Structure

IRDye® 800CW Azide Infrared Dye

Properties

  • Chemical Formula: C54H67N6Na3O17S4
  • Molecular Weight: 1269.36 g/mol
  • Exact Mass: 1268.31
  • Correction factor at 260 – 280 nm: 3%

Absorption and Emission Spectra

Solvent Absorption Maxima Emission Maxima
Methanol 778 nm 794 nm
1X PBS 774 nm 789 nm
1:1 Methanol to 1X PBS 778 nm

Absorbance and Emission Spectra in 1X PBS

IRDye® 800CW Azide Infrared Dye

Molar Absorptivity

Solvent Molar Absorptivity (L mol-1 cm-1)
Methanol 300,000
1X PBS 240,000
1:1 Methanol to 1X PBS 270,000

Example Data

IRDye® 800CW Azide Infrared Dye
Palmitoylation of Lck kinase analyzed with a Click Chemistry IRDye reagent.​​ Lck was immunoprecipitated from metabolically-labeled Jurkat cell lysates. Palmitoylation was detected with CuAAC click chemistry and IRDye 800CW Azide Infrared Dye​ (green signals; P/N 929-60000). Total Lck was detected with anti-Lck D88 antibody and IRDye 680RD Donkey anti-Rabbit IgG​ (red; P/N 926-68073). Yellow indicates overlap of signals. The mobility shift caused by palmitoylation is observed in lanes 3-5 and 7-9 (green bands migrating more slowly than red bands). Data and image supplied by a LI-COR customer.
使用 Click Chemistry IRDye 试剂分析 Lck 激酶的棕榈酰化。Lck 从代谢标记的 Jurkat 细胞裂解物中进行免疫沉淀。 使用 CuAAC 点击化学和 IRDye 800CW 叠氮化物红外染料（绿色信号；P/N 929-60000）检测棕榈酰化。 使用抗 Lck D88 抗体和 IRDye 680RD 驴抗兔 IgG（红色；P/N 926-68073）检测总 Lck。 黄色表示信号重叠。 在泳道 3-5 和 7-9 中观察到由棕榈酰化引起的迁移率变化（绿色条带迁移速度比红色条带慢）。 LI-COR 客户提供的数据和图像。

LI-COR IRDye® 800CW 山羊抗人 IgG 二抗,LI-COR IRDye® 800CW

Reagents > IRDye® 800CW Goat anti-Human IgG Secondary Antibody,

IRDye® 800CW Goat anti-Human IgG Secondary Antibody

Immunogen

Human IgG

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using human IgG covalently linked to agarose. Based on ELISA, this antibody reacts with the heavy and light chains of human IgG. This antibody was tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, horse, and mouse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

Applications

Highly recommended for:

  • Western Blot
  • In-Cell Western™ Assay
  • On-Cell Western Assay
  • Protein Array
  • Immunohistochemistry
  • Small Animal Imaging
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging
  • Virus Titration Assay

Formulation

IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 800CW 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:15,000 1:5,000 – 1:25,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

RRID

  • P/N 925-32232: RRID AB_2814904
  • P/N 926-32232: RRID AB_10806644

Example Data

IRDye® 800CW Goat anti-Human IgG Secondary Antibody
Detection of human IgG with IRDye 800CW Goat anti-Human IgG. Serial dilutions of human IgG were spiked into C32 lysate (Santa Cruz P/N sc-2205). Samples were resolved on a 10% Bis-Tris gel and transferred to Odyssey® Nitrocellulose membrane (P/N 926-31092). Membrane was blocked with Odyssey Blocking Buffer (P/N 927-40000) followed by detection with IRDye 800CW Goat anti-Human IgG (P/N 926-32232).

LI-COR IRDye® 800CW 马来酰亚胺,LI-COR IRDye® 800CW

Reagents > IRDye® 800CW Maleimide,IRDye® 800CW 马来酰亚胺

IRDye® 800CW Maleimide

IRDye 800CW Maleimide provides the functionality for labeling molecules that contain free sulfhydryl (–SH) groups, such as cysteine residues in proteins, with IRDye 800CW. The reactive group of the IRDye 800CW Maleimide allows conjugation reactions to be performed very efficiently at physiological pH.

IRDye 800CW 马来酰亚胺提供了用 IRDye 800CW 标记含有游离巯基 (-SH) 基团的分子的功能，例如蛋白质中的半胱氨酸残基。 IRDye 800CW 马来酰亚胺的反应性基团允许在生理 pH 值下非常有效地进行偶联反应。

IRDye® 800CW Maleimide
Maleimide reacting with a thiol group.

Dye Structure

IRDye® 800CW Maleimide

Properties

  • Chemical Formula: C52H57N4Na3O16S4
  • Molecular Weight: 1191.25 g/mol
  • Exact Mass: 1190.23

Absorption and Emission Spectra

Solvent Ext. Coeff. (M-1cm-1) Absorption Maxima Emission Maxima
Methanol 300,000 778 nm 794 nm
Water 240,000 774 nm 789 nm
1X PBS 240,000 774 nm 789 nm
PBS: Methanol 270,000 777 nm 791 nm

Absorption and Emission Spectra in 1X PBS

IRDye® 800CW Maleimide

LI-COR IRDye® 800CW 蛋白质标记试剂盒,LI-COR IRDye 800CW

Reagents > IRDye® 800CW Protein Labeling Kits,IRDye® 800CW 蛋白质标记试剂盒

IRDye® 800CW Protein Labeling Kits

IRDye 800CW Protein Labeling Kits can be used to label antibodies and other proteins for applications such as Western blots, In-Cell Western™ assays, in vivo imaging, and whole organ or tissue section assays.

IRDye 800CW 蛋白质标记试剂盒可用于标记抗体和其他蛋白质，用于蛋白质印迹、In-Cell Western™ 测定、体内成像以及整个器官或组织切片测定等应用。

High Molecular Weight

Optimized for labeling 1.0 mg amounts of protein with molecular weight 45 – 200 kDa.

针对标记 1.0 mg 分子量 45 – 200 kDa 的蛋白质进行了优化。

This kit contains:

    • 3 x 0.1 mg IRDye 800CW Reactive Dye vials
    • 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
    • 25 mL 1X PBS
    • 0.5 mL ultra-pure water
    • 3 x Pierce® Zeba™ Desalting Spin Columns (P/N 89891)

Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.

  • Pierce Zeba Desalting Spin column instructions
  • Protocol for IRDye 800CW Protein Labeling Kit – High MW

Low Molecular Weight

Optimized for labeling 1.0 mg of protein with molecular weight 14 – 45 kDa.

This kit contains:

    • 3 x 0.5 mg IRDye 800CW Reactive Dye vials
    • 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
    • 25 mL 1X PBS
    • 0.5 mL ultra-pure water
    • 3 x Pierce Zeba Desalting Spin Columns (P/N 89891)

Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.

  • Pierce Zeba Desalting Spin column instructions
  • Protocol for IRDye 800CW Protein Labeling Kit – Low MW

Microscale

Optimized for labeling 100 μg of protein with molecular weight 14 – 200 kDa.

This kit contains:

    • 3 x 0.1 mg IRDye 800CW Reactive Dye vials
    • 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
    • 25 mL 1X PBS
    • 0.5 mL ultra-pure water
    • 3 x Pierce Zeba Desalting Spin Columns (P/N 89892)

Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.

  • Pierce Zeba Desalting Spin Column instructions
  • Protocol for IRDye 800CW Protein Labeling Kit – Microscale

LI-COR IRDye® 800CW 链霉亲和素,LI-COR IRDye® 800CW

Reagents > IRDye® 800CW Streptavidin,IRDye® 800CW 链霉亲和素

IRDye® 800CW Streptavidin

IRDye 800CW Streptavidin is supplied as a liquid in buffer containing 10 mM phosphate, 183 mM NaCl, 2.7 nM KCl, pH 7.4 with sodium azide 0.005% (w/v) as a preservative.

To use, centrifuge briefly before use to eliminate aggregates that may have formed in solution. This will reduce non-specific background staining. A final concentration of 0.2 to 1.0 µg/ml (1:1,000 to 1:5,000) is usually satisfactory for most applications; however, appropriate dilution may need to be determined empirically.

For membrane-based applications and In-Gel Westerns, it is recommended to add SDS (0.02% to 0.1% final concentration), in addition to Tween® 20 (0.1 to 0.2% final concentration) during the detection incubation step to reduce non-specific background staining.

IRDye 800CW 链霉亲和素以液体形式提供，缓冲液中含有 10 mM 磷酸盐、183 mM NaCl、2.7 nM KCl，pH 7.4，叠氮化钠 0.005% (w/v) 作为防腐剂。

要使用，请在使用前短暂离心以消除可能在溶液中形成的聚集体。 这将减少非特异性背景染色。 对于大多数应用，0.2 到 1.0 µg/ml（1:1,000 到 1:5,000）的最终浓度通常是令人满意的； 但是，可能需要根据经验确定适当的稀释度。

对于基于膜的应用和 In-Gel Westerns，建议在检测孵育步骤中添加 SDS（0.02% 至 0.1% 最终浓度）和 Tween® 20（0.1 至 0.2% 最终浓度）以减少非 特定背景染色。

Recommended Dilutions

Application Suggested Range Tween 20* SDS*
Odyssey® Western blot detection 1:1,000 – 1:5,000 0.1 – 0.2% (v/v) 0.02 – 0.1% (v/v)
Other User optimized User optimized User optimized

Optimum dilutions will vary and should be determined empirically.

* Added to reduce non-specific background staining.

See the complete line of IRDye Streptavidins.