标签：LI-COR IRDye® 680LT 驴抗山羊 IgG 二抗

LI-COR IRDye® 680LT 驴抗山羊 IgG 二抗,LI-COR IRDye 680LT 二抗

Reagents > IRDye® 680LT Donkey anti-Goat IgG Secondary Antibody,IRDye® 680LT 驴抗山羊 IgG 二抗

IRDye® 680LT Donkey anti-Goat IgG Secondary Antibody

Immunogen

Goat IgG

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using goat IgG covalently linked to agarose. Based on ELISA, this antibody reacts with the heavy and light chains of goat IgG and sheep IgG. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with human, mouse, rabbit, rat, chicken. guinea pig, hamster, swine, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

使用与琼脂糖共价连接的山羊 IgG 通过亲和层析实现特异性抗体的分离。 基于 ELISA，该抗体与山羊 IgG 和绵羊 IgG 的重链和轻链反应。 该抗体已通过斑点印迹和/或固相吸附测试，以确保与人、小鼠、兔、大鼠、鸡的交叉反应最小。 豚鼠、仓鼠、猪和马血清蛋白，但可能与其他物种的免疫球蛋白发生交叉反应。 该偶联物经过专门测试，可用于蛋白质印迹应用。

Applications

Recommended for:

  • Western Blot
  • Protein Array
  • Immunohistochemistry
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging

Not Recommended for:

  • Small Animal Imaging
  • In-Cell Western Assay
  • On-Cell Western Assay

Note: Do not use this secondary antibody in combination with any secondary antibody whose host is goat (for example, IRDye 800CW Goat anti-Rabbit) for two-color Western blot detection.

Formulation

IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 680LT 二抗以纯化的免疫球蛋白偶联物形式提供，在磷酸盐缓冲盐水中冻干，pH 7.4。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:20,000 1:20,000 – 1:50,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

Note: When using PVDF membranes for Western blotting applications, SDS (final concentration of 0.01 – 0.02%) and Tween® 20 (final concentration of 0.1 – 0.2%) must be added during the detection incubation step to avoid non-specific background staining.

RRID

  • P/N 925-68024: RRID AB_2814908
  • P/N 926-68024: RRID AB_10706168

Example Data

IRDye® 680LT Donkey anti-Goat IgG Secondary Antibody
Western blot detection of actin in C32 lysate. C32 lysate (Santa Cruz P/N sc- 2205) was resolved on a 10% Bis-Tris gel and transferred to Odyssey® nitrocellulose (P/N 926-31090). The membrane was probed with goat anti-actin (Santa Cruz P/N sc-1616) followed by detection with IRDye 680LT Donkey anti-Goat IgG (P/N 926-68024).
蛋白质印迹检测 C32 裂解物中的肌动蛋白。 C32 裂解物 (Santa Cruz P/N sc-2205) 在 10% Bis-Tris 凝胶上分离并转移到 Odyssey® 硝酸纤维素 (P/N 926-31090)。 用山羊抗肌动蛋白 (Santa Cruz P/N sc-1616) 探测膜，然后用 IRDye 680LT 驴抗山羊 IgG (P/N 926-68024) 检测。