标签：LI-COR IRDye® 680LT 驴抗兔 IgG 二抗

Immunogen

Rabbit IgG, whole molecule

Purity and Specificity

The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG, and with the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

使用与琼脂糖珠偶联的抗原，通过免疫亲和层析从抗血清中分离抗体。 基于免疫电泳，该抗体与兔 IgG 的重链以及大多数兔免疫球蛋白常见的轻链反应。 未检测到针对非免疫球蛋白血清蛋白的反应性。 该抗体经过 ELISA 和/或固相吸附测试，以确保与牛、鸡、山羊、豚鼠、叙利亚仓鼠、马、人、小鼠、大鼠和绵羊血清蛋白的交叉反应最小，但可能发生交叉反应 与来自其他物种的免疫球蛋白。 该偶联物经过专门测试，可用于蛋白质印迹应用。

Applications

Recommended for:

• Western Blot
• Protein Array
• Immunohistochemistry
• Microscopy
• 2D Gel Detection
• Tissue Section Imaging

Not Recommended for:

• Small Animal Imaging
• In-Cell Western Assay
• On-Cell Western Assay

Formulation

IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 680LT 二抗以纯化的免疫球蛋白偶联物形式提供，在磷酸盐缓冲盐水中冻干，pH 7.4。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Optimum dilutions will vary and should be determined empirically.

RRID

• P/N 925-68023: RRID AB_2814907
• P/N 926-68023: RRID AB_10706167