The Compound Injection Clip helps secure a syringe/catheter system for administering compounds to small animals during imaging. It attaches to the Pearl® Imaging Bed (P/N 9300-21) for easy transfer from work area to instrument.
The Clip accommodates syringes with OD 0.22-0.3 inches and enables injection of compounds even when the imager drawer is closed.
GAM CellTag 700 Stain ICW Kits provide detection reagents for cell-based In-Cell Western™ Assays. This cost-effective normalization method makes quantification of the target protein more precise. CellTag 700 Stain accumulates in the nucleus and cytoplasm of permeabilized cells allowing for accurate normalization to cell number.
This kit includes reagents to perform an In-Cell Western assay and CellTag 700 Stain to normalize well-to-well variations in cell number. CellTag 700 accumulates in the nucleus and cytoplasm of permeabilized cells allowing for accurate normalization to cell number.
Accessories > MPX™ Blotting System for Multiple-Target Western Blots,用于多靶点蛋白质印迹的 MPX™ 印迹系统
The MPX Blotting System is ideal for any multiple-target Western blot application. This system increases Western blot throughput, maximizes efficiency, and reduces relative overall cost.
Screen Samples or Optimize Blotting Conditions with the MPX Blotting System
Screen multiple samples and multiple targets all on the same blot without cutting, stripping, or cross-contamination.
Use the MPX Blotter for a variety of assays, such as primary and secondary antibody screening, monoclonal antibody screening, blocking buffer optimization as well as multi-target probing of 1 – 4 samples on a single membrane. Most common Western procedures that generate a PVDF or nitrocellulose blot of 7 cm x 8.5 cm can be adapted to the MPX format.
在同一个印迹上筛选多个样品和多个靶标，无需切割、剥离或交叉污染。
使用 MPX Blotter 进行各种检测，例如一抗和二抗筛选、单克隆抗体筛选、封闭缓冲液优化以及单膜上 1-4 个样本的多靶点探测。 产生 7 cm x 8.5 cm 的 PVDF 或硝酸纤维素印迹的最常见的西方程序可以适应 MPX 格式。
Simple, Convenient Workflow
Channel ports are conveniently spaced, staggered, and beveled to create a simple workflow for using both standard and multi-channel pipettes. The low volume channel ports accommodate a maximum volume of 160 µL, thus conserving precious and costly antibody. The nesting dovetail joint design allows multiple MPX units to be linked together for easy-to-handle multiple-blot processing.
Screen Up To 48 Targets on One Blot
Processing is as simple as clamping the blot into the MPX, creating up to 24 independent channels. The range of usable channels per sample is relative to comb-size – from 24 on a single sample to four on a four-sample blot.
Using LI-COR’s selection of IRDye® dye-conjugated secondary antibodies on the Odyssey Imagers provides a maximum of 48-targets on a single membrane— two per channel—and the option for quantitative analysis. Other detection methods, such as standard chemiluminescence and colorimetric, can provide a maximum 24 targets.
MPX Blotting System Specifications
Overall Size: 15.5 cm x 8.5 cm x 3.5 cm (L x W x H)
Membrane Size: 7 cm x 8.5 cm (H x L)
Channel Dimensions: 50 mm x 1.5 mm x 1.5 mm (L x W x H)
Number of Channels: 24
Total Volume per Channel Including Port: 160 µL
MPX Western Blot with One Sample and Multiple Targets
Representative MPX Western Blot with one sample and multiple targets.
The Odyssey Pen is suitable for writing on membranes to be scanned on any of the Odyssey Imagers.
Inks from other pens will autofluoresce in the 700 nm channel, potentially adding to background or interfering artifacts on your image. The Odyssey Pen does not.
A 96-well verification plate for Operational Qualification (OQ) on the Odyssey CLx or Odyssey Sa imagers. OQ services sold separately. The Odyssey Verification Plate is used during the OQ procedure to ensure data consistency, but can also be used by the customer to ensure that the Odyssey CLx or Odyssey Sa is performing as expected over time.
用于 Odyssey CLx 或 Odyssey Sa 成像仪上的操作认证 (OQ) 的 96 孔验证板。 OQ 服务单独出售。 Odyssey 验证板在 OQ 过程中使用以确保数据一致性，但客户也可以使用它来确保 Odyssey CLx 或 Odyssey Sa 在一段时间内按预期运行。
A 96-well verification plate for Operational Qualification (OQ) on the Odyssey DLx, Odyssey CLx or Odyssey Sa imagers. OQ services sold separately. The Odyssey Verification Plate is used during the OQ procedure to ensure data consistency, but can also be used by the customer to ensure that the Odyssey DLx, Odyssey CLx or Odyssey Sa is performing as expected over time.
用于 Odyssey DLx、Odyssey CLx 或 Odyssey Sa 成像仪上的操作认证 (OQ) 的 96 孔验证板。 OQ 服务单独出售。 Odyssey 验证板在 OQ 过程中使用以确保数据一致性，但客户也可以使用它来确保 Odyssey DLx、Odyssey CLx 或 Odyssey Sa 在一段时间内按预期运行。
Reagents > Odyssey® One-Color Protein Molecular Weight Marker,Odyssey® 单色蛋白质分子量标记
The Odyssey One-Color Protein MW Marker provides a ladder of convenient, consistent sizes for both visible viewing and 700 nm channel for near-infrared detection.
Includes ten protein bands from 10 to 250 kDa.
Has three reference bands at 25, 50, and 75 kDa that are three times as intense as other bands.
Not sure which protein marker to choose? Visit How to Choose the Right Protein Ladder.
Odyssey One-Color Protein MW Marker 为可见光观察和用于近红外检测的 700 nm 通道提供了方便、一致的尺寸阶梯。
Representative images of the Odyssey One-Color Marker on a NuPAGE® Novex 10% Bis-Tris gel (2 μL loading volume). Image generated using a digital camera (left) and Odyssey CLx Infrared Imaging System (center, right).
These silicone mats are used for flattening membranes against the glass surface on the Odyssey® DLx, Odyssey M, Odyssey CLx or Odyssey Classic Infrared Imaging System.
The mats come in three sizes: 25 cm x 25 cm, 13 cm x 13 cm, and 19 cm x 26 cm. The 19 cm x 26 cm size is specific for the Odyssey M Imager.
Western Blot Storage Bags are ideal for long-term storage of near-infrared fluorescent Western blot membranes. These bags protect blotted membranes from light, scratching, and photobleaching of fluorophores for later reprobing (detection of different targets), imaging, and documentation purposes.
Both nitrocellulose and PVDF membranes treated with primary and secondary antibodies can be stored dry for later imaging and documentation.
For blots that are being stored for reprobing, it is recommended that membranes be stripped first, and dried prior to storage.
Software > Western Key (Lite/C-DiGit®),西键 (Lite/C-DiGit®)
This is an optional key available for purchase separately with the following software:
Image Studio™ Software for the C-DiGit® Blot Scanner
Image Studio Lite Software
With this key, you can analyze two-color Western blot and multiplex Western blot images using advanced analysis tools for normalization, sizing, and quantitation.
Note: The C-DiGit Blot Scanner cannot image two-color Western blots, but the software can import images acquired with other LI-COR instruments for analysis.
这是一个可选密钥，可与以下软件一起单独购买：
用于 C-DiGit® 印迹扫描仪的 Image Studio™ 软件
Image Studio 精简版软件
使用此密钥，您可以使用高级分析工具分析双色蛋白质印迹和多重蛋白质印迹图像，以进行标准化、大小调整和定量。
注意：C-DiGit Blot Scanner 无法对双色 Western blot 进行成像，但该软件可以导入使用其他 LI-COR 仪器采集的图像进行分析。
