标签：LI-COR 二抗

LI-COR 二抗,LI-COR P/N 925-68070,IRDye® 680RD 山羊抗小鼠 IgG 二抗

Reagents > IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody,IRDye® 680RD 山羊抗小鼠 IgG 二抗

IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody

Immunogen

Mouse IgG paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA. This antibody was tested by dot blot and and/or solid-phase adsorbed for minimal cross-reactivity with human, rabbit, goat, rat, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications.

使用与琼脂糖共价连接的混合小鼠 IgG 通过亲和层析实现特异性抗体的分离。 基于 ELISA 和流式细胞术，该抗体与小鼠 IgG1、IgG2a、IgG2b 和 IgG3 的重链和轻链以及小鼠 IgM 和 IgA 的轻链反应。 通过斑点印迹和/或固相吸附测试该抗体与人、兔、山羊、大鼠和马血清蛋白的交叉反应最小，但可能与来自其他物种的免疫球蛋白交叉反应。 该偶联物经过专门测试，可用于蛋白质印迹和 In-Cell Western™ 检测应用。

Applications

Highly recommended for:

  • Western Blot
  • In-Cell Western Assay
  • On-Cell Western Assay
  • Protein Array
  • Immunohistochemistry
  • Small Animal Imaging
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging

Formulation

IRDye 680RD secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 680RD 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application Recommended Suggested Range
Odyssey® Western blot detection 1:15,000 1:5,000 – 1:25,000
In-Cell Western assay detection 1:800 1:200 – 1:800
Other User optimized

Optimum dilutions will vary and should be determined empirically.

RRID

  • P/N 925-68070: RRID AB_2651128
  • P/N 926-68070: RRID AB_10956588

LI-COR 二抗, On-Cell Western 检测,IRDye® 680RD 山羊抗大鼠 IgG 二抗

Reagents > IRDye® 680RD Goat anti-Rat IgG Secondary Antibody,IRDye® 680RD 山羊抗大鼠 IgG 二抗

IRDye® 680RD Goat anti-Rat IgG Secondary Antibody

Immunogen

Rat IgG, whole molecule

Purity and Specificity

The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule rat IgG, and with the light chains of other rat immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse, bovine, horse, human, and rabbit serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications.

使用与琼脂糖珠偶联的抗原，通过免疫亲和层析从抗血清中纯化抗体。 基于免疫电泳和/或 ELISA，该抗体与全分子大鼠 IgG 以及其他大鼠免疫球蛋白的轻链发生反应。 未检测到针对非免疫球蛋白血清蛋白的反应性。 该抗体通过 ELISA 和/或固相吸附测试，以确保与小鼠、牛、马、人类和兔血清蛋白的交叉反应最小，但可能与来自其他物种的免疫球蛋白交叉反应。 该偶联物经过专门测试，可用于蛋白质印迹和 In-Cell Western™ 检测应用。

Applications

Highly recommended for:

  • Western Blot
  • In-Cell Western Assay
  • On-Cell Western Assay
  • Protein Array
  • Immunohistochemistry
  • Small Animal Imaging
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging
  • 蛋白质印迹
    细胞内西方检测
    On-Cell Western 检测
    蛋白质阵列
    免疫组织化学
    小动物成像
    显微镜
    2D 凝胶检测
    组织切片成像

Formulation

IRDye 680RD secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 680RD 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application Recommended Suggested Range
Odyssey® Western blot detection 1:15,000 1:5,000 – 1:25,000
In-Cell Western assay detection 1:800 1:200 – 1:800
Other User optimized

Optimum dilutions will vary and should be determined empirically.

RRID

  • P/N 925-68076: RRID AB_2814913
  • P/N 926-68076: RRID AB_10956590

LI-COR IRDye® 800CW 山羊抗小鼠 IgM（μ 链特异性）二抗,LI-COR 二抗

Reagents > IRDye® 800CW Goat anti-Mouse IgM (µ chain specific) Secondary Antibody,IRDye® 800CW 山羊抗小鼠 IgM（μ 链特异性）二抗

IRDye® 800CW Goat anti-Mouse IgM (µ chain specific) Secondary Antibody

Immunogen

Mouse IgM paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgM covalently linked to agarose. Based on ELISA and/or flow cytometry, this antibody reacts with the heavy chain of mouse IgM. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgG1, IgG2a, IgG3, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications.

使用与琼脂糖共价连接的小鼠 IgM 通过亲和层析实现特异性抗体的分离。 基于 ELISA 和/或流式细胞术，该抗体与小鼠 IgM 的重链发生反应。 该抗体已通过斑点印迹和/或固相吸附测试，以确保与小鼠 IgG1、IgG2a、IgG3 和 IgA、合并的人血清和纯化的人副蛋白的交叉反应最小。 该偶联物经过专门测试，可用于蛋白质印迹应用。

Applications

Highly recommended for:

  • Western Blot
  • In-Cell Western™ Assay
  • On-Cell Western Assay
  • Protein Array
  • Immunohistochemistry
  • Small Animal Imaging
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging
  • Virus Titration Assay
  • 蛋白质印迹
    In-Cell Western™ 检测
    On-Cell Western 检测
    蛋白质阵列
    免疫组织化学
    小动物成像
    显微镜
    2D 凝胶检测
    组织切片成像
    病毒滴定法

Formulation

IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 800CW 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application Recommended Suggested Range
Odyssey® Western blot detection 1:15,000 1:5,000 – 1:25,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

RRID

  • P/N 925-32280: RRID AB_2814916
  • P/N 926-32280: RRID AB_2814919