标签：IRDye 800CW 2-脱氧葡萄糖

LI-COR IRDye® 800CW 2-DG 光学探头,IRDye 800CW 2-脱氧葡萄糖

Reagents > IRDye® 800CW 2-DG Optical Probe,IRDye® 800CW 2-DG 光学探头

IRDye® 800CW 2-DG Optical Probe

IRDye 800CW 2-DG (2-deoxyglucose) is a fluorescent optical imaging agent that has been shown to be reactive with implanted tumors derived from many cell lines including A431, SW620, 3T3-L1, and PC3LMN4. This optical imaging agent has been used for numerous applications, including research pertaining to tumor biology, tumor metastases, diabetes, and arthritis.

Cancer cells are often characterized by a high metabolic rate exemplified by an elevated rate of glycolysis. This observation forms the basis for positron emission tomography (PET) using glucose analogues such as 18F-2-deoxy-D-glucose (18FDG) to visualize primary tumors and their metastasis. Radiolabeled 2-deoxy-D-glucose (2-DG) is generally impractical for use in small animal studies of cancer biology. Fluorophore-labeled variants of 2-deoxy-D-glucose have been used with varying degrees of success.1, 2, 3

The optical agent exhibits the expected dose response with increasing concentrations of the agent in both cell based and animal experiments. Uptake of the agent can be effectively blocked with either unlabeled 2-DG or glucose, confirming its specificity.

Confocal microscopy demonstrated conclusively that IRDye 800CW 2-DG is taken up by the cell and localized in the cytoplasm. While the exact mechanism of IRDye 800CW 2-DG uptake is not known, there is strong evidence for the involvement of the GLUT transporter system.

IRDye 800CW 2-DG（2-脱氧葡萄糖）是一种荧光光学成像剂，已被证明与来自许多细胞系（包括 A431、SW620、3T3-L1 和 PC3LMN4）的植入肿瘤发生反应。这种光学显像剂已用于许多应用，包括与肿瘤生物学、肿瘤转移、糖尿病和关节炎有关的研究。

癌细胞通常以高代谢率为特征，例如糖酵解率升高。该观察结果构成了使用葡萄糖类似物（例如 18F-2-脱氧-D-葡萄糖 (18FDG)）可视化原发性肿瘤及其转移的正电子发射断层扫描 (PET) 的基础。放射性标记的 2-脱氧-D-葡萄糖 (2-DG) 通常不适用于癌症生物学的小动物研究。使用荧光团标记的 2-脱氧-D-葡萄糖变体已取得不同程度的成功。 1, 2, 3

在基于细胞的实验和动物实验中，随着试剂浓度的增加，光学试剂表现出预期的剂量反应。未标记的 2-DG 或葡萄糖可以有效地阻断该试剂的摄取，从而证实了其特异性。

共聚焦显微镜最终证明 IRDye 800CW 2-DG 被细胞吸收并定位于细胞质中。虽然 IRDye 800CW 2-DG 摄取的确切机制尚不清楚，但有强有力的证据表明 GLUT 转运系统参与其中。

Learn about other LI-COR optical probes.

Applications

  • Microscopy
  • Optical Probe Development
  • Target Specificity
  • Tissue Section Imaging
  • 显微镜
    光学探头开发
    目标特异性
    组织切片成像

Absorbance and Emission Spectra

IRDye® 800CW 2-DG Optical Probe

Data Example

IRDye® 800CW 2-DG Optical Probe
Two examples in which IRDye 800CW 2-DG Optical Probe (10 nmol) was used to detect subcutaneous A431 tumors. Probe signal is displayed in pseudo color and is merged with a grey scale white light image of the mouse.
使用 IRDye 800CW 2-DG 光学探头 (10 nmol) 检测皮下 A431 肿瘤的两个示例。 探针信号以伪彩色显示，并与鼠标的灰度白光图像合并。

References

  1. Lloyd, P.G., Hardin, C.D., and Sturek, M. (1999). Physiol Res, 48. 401-10.
  2. O’Neil, R.G., Wu, L., and Mullani, N. (2005) Mol Imaging Biol, 7. 388-92.
  3. Cheng, Z., Levi, J., Xiong, Z., Gheysens, O., Keren, S., Chen, X., and Gambhir, S.S. (2006) Bioconjug Chem, 17. 662-9.
  4. Kovar, J. L., Volcheck, W. M., Sevick-muraca, E., Simpson, M. A., & Olive, D. M. (2009). Anal Biochem, 384(2). 254-262. doi: 10.1016/j.ab.2008.09.050.