标签：IRDye® 680RD 蛋白质标记试剂盒

IRDye 680RD Protein Labeling Kits can be used to label antibodies and other proteins for Western blotting, In-Cell Western™ assays, in vivo imaging, and whole organ or tissue section applications.

IRDye 680RD 蛋白质标记试剂盒可用于标记抗体和其他蛋白质，用于蛋白质印迹、In-Cell Western™ 测定、体内成像以及整个器官或组织切片应用。

High Molecular Weight高分子量

Optimized for labeling 1.0 mg of protein with molecular weight 45 – 200 kDa.

This kit contains:

• • 3 x 0.1 mg IRDye 680RD Reactive Dye vials
  • 0.5 mL 1 M Potassium Phosphate (K₂HPO₄), pH 9
  • 25 mL 1X PBS
  • 0.5 mL ultra-pure water
  • 3 x Pierce^® Zeba™ Desalting Spin Columns (P/N 89891)

Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.

注意：这些色谱柱推荐的最小蛋白质分子量为 7 kDa。 有关选项，请参阅 IRDye 肽标记指南。

• Pierce Zeba Desalting Spin column instructions
• Protocol for IRDye 680RD Protein Labeling Kit – High MW

Low Molecular Weight低分子量

Optimized for labeling 1.0 mg of protein with molecular weight 14 – 45 kDa.

This kit contains:

• • 3 x 0.5 mg IRDye 680RD Reactive Dye vials
  • 0.5 mL 1 M Potassium Phosphate (K₂HPO₄), pH 9
  • 25 mL 1X PBS
  • 0.5 mL ultra-pure water
  • 3 x Pierce Zeba Desalting Spin Columns (P/N 89891)

Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.

• Pierce Zeba Desalting Spin column instructions
• Protocol for IRDye 680RD Protein Labeling Kit – Low MW

Microscale微量

Optimized for labeling 100 μg of protein with molecular weight 14 – 200 kDa.

This kit contains:

• • 3 x 0.1 mg IRDye 680RD Reactive Dye vials
  • 0.5 mL 1 M Potassium Phosphate (K₂HPO₄), pH 9
  • 25 mL 1X PBS
  • 0.5 mL ultra-pure water
  • 3 x Pierce Zeba Desalting Spin Columns (P/N 89892)

Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.

• Pierce Zeba Desalting Spin Column instructions
• Protocol for IRDye 680RD Protein Labeling Kit – Microscale