Reagents > IRDye® 680LT NHS Ester,IRDye® 680LT NHS 酯
IRDye infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence.
Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins.
Which 700 Channel Dye Should I Use?
Note: IRDye 680LT dye products should not be used for small animal in vivo imaging or In‑Cell Western™ Assays. The higher level of fluorescent intensity creates high background making it unfavorable for use in these applications. We recommend using IRDye 680RD dye products for these applications.
LI-COR Biosciences 的 IRDye 红外染料可供研究人员使用近红外 (NIR) 荧光开发应用。
标准 NHS 酯化学用于生产用 LI-COR IRDye 红外染料标记的定制探针。 NHS 酯反应基团提供了标记伯胺和仲胺的功能，例如蛋白质中的赖氨酸残基。
我应该使用哪种 700 通道染料？
注意：IRDye 680LT 染料产品不应用于小动物体内成像或 In-Cell Western™ 检测。 较高水平的荧光强度会产生高背景，使其不利于在这些应用中使用。 我们建议在这些应用中使用 IRDye 680RD 染料产品。
Dye Structure
Properties
- Chemical Formula: C54H52N3Na3O22S6
- Molecular Weight: 1402.32 g/mol
- Exact Mass: 1401.09
Absorption and Emission Spectra
| Solvent | Ext. Coeff. (M-1cm-1) | Absorption Maxima | Emission Maxima |
|---|---|---|---|
| Methanol | 250,000 | 680 nm | 694 nm |
| 1X PBS | 250,000 | 676 nm | 693 nm |
Absorption and Emission Spectra in 1X PBS
