Reagents > IRDye® 680LT Maleimide
IRDye 680LT Maleimide will label molecules with free sulfhydryl (–SH) groups, such as cysteine residues in proteins. Conjugation can be performed at physiological pH.
Which 700 Channel Dye Should I Use?
Note: IRDye 680LT dye products should not be used for small animal in vivo imaging or In‑Cell Western™ Assays. The higher level of fluorescent intensity creates high background making it unfavorable for use in these applications. We recommend using IRDye 680RD dye products for these applications.
IRDye 680LT 马来酰亚胺将标记具有游离巯基 (-SH) 基团的分子，例如蛋白质中的半胱氨酸残基。 缀合可以在生理pH下进行。
我应该使用哪种 700 通道染料？
注意：IRDye 680LT 染料产品不应用于小动物体内成像或 In-Cell Western™ 检测。 较高水平的荧光强度会产生高背景，使其不利于在这些应用中使用。 我们建议在这些应用中使用 IRDye 680RD 染料产品。
Dye Structure
Properties
- Chemical Formula: C56H55N5Na5O21S6
- Molecular Weight: 1427.37 g/mol
- Exact Mass: 1426.12
Absorption and Emission Spectra
| Solvent | Ext. Coeff. (M-1cm-1) | Absorption Maxima | Emission Maxima |
|---|---|---|---|
| Methanol | 250,000 | 680 nm | 694 nm |
| 1X PBS | 250,000 | 676 nm | 693 nm |
Absorption and Emission Spectra in 1X PBS
