标签：IRDye® 680LT 山羊抗小鼠 IgG2b 特异性二抗

LI-COR P/N 926-68052,IRDye® 680LT 山羊抗小鼠 IgG2b 特异性二抗

Reagents > IRDye® 680LT Goat anti-Mouse IgG2b-Specific Secondary Antibody

IRDye® 680LT Goat anti-Mouse IgG2b-Specific Secondary Antibody

Immunogen

Mouse IgG2b paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography on mouse IgG2b covalently linked to agarose. Based on ELISA and flow cytometry, the antibody reacts with the heavy chain of mouse IgG2b. This antibody was tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgM, IgG1, IgG2a, IgG3, and IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications. Results with your primary antibody may vary and specificity should be confirmed prior to performing two-color detection.

Applications

Recommended for:

  • Western Blot
  • Protein Array
  • Immunohistochemistry
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging

Not Recommended for:

  • Small Animal Imaging
  • In-Cell Western Assay
  • On-Cell Western Assay

Formulation

IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 680LT 二抗以纯化的免疫球蛋白偶联物形式提供，在磷酸盐缓冲盐水中冻干，pH 7.4。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:20,000 1:20,000 – 1:50,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

Note: When using PVDF membranes for Western blotting applications, SDS (final concentration of 0.01 – 0.02%) and Tween® 20 (final concentration of 0.1 – 0.2%) must be added during the detection incubation step to avoid non-specific background staining.

RRID

  • P/N 926-68052: RRID AB_2783644

Example Data

IRDye® 680LT Goat anti-Mouse IgG2b-Specific Secondary Antibody
Western blot detection of p53 in various lysates. Lanes 1-5: Odyssey® One-Color Molecular Weight Marker (P/N 928-40000), Hela, COS7, A431, and C32 cell lysates. The membrane was blocked with Odyssey Blocking Buffer (P/N 927-40000) and probed with mouse anti-p53 (IgG2a) (Santa Cruz P/N sc-126) and Mouse anti-β Actin (IgG2b) (P/N 926-42212) followed by detection with IRDye 800CW Goat anti-Mouse IgG2a (P/N 926-32351) and IRDye 680LT Goat anti-Mouse IgG2b (P/N 926-68052).
各种裂解物中 p53 的蛋白质印迹检测。 泳道 1-5：Odyssey® One-Color Molecular Weight Marker (P/N 928-40000)、Hela、COS7、A431 和 C32 细胞裂解物。 用 Odyssey 封闭缓冲液 (P/N 927-40000) 封闭膜，并用小鼠抗 p53 (IgG2a) (Santa Cruz P/N sc-126) 和小鼠抗β肌动蛋白 (IgG2b) (P/N 926) 进行探测 -42212)，然后使用 IRDye 800CW 山羊抗小鼠 IgG2a (P/N 926-32351) 和 IRDye 680LT 山羊抗小鼠 IgG2b (P/N 926-68052) 进行检测。