标签：IRDye® 680LT 山羊抗小鼠 IgG1 特异性二抗

LI-COR P/N 926-68050,IRDye® 680LT 山羊抗小鼠 IgG1 特异性二抗

Reagents > IRDye® 680LT Goat anti-Mouse IgG1-Specific Secondary Antibody,IRDye® 680LT 山羊抗小鼠 IgG1 特异性二抗

IRDye® 680LT Goat anti-Mouse IgG1-Specific Secondary Antibody

Immunogen

Mouse IgG1 paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography on mouse IgG1 covalently linked to agarose. Based on ELISA and flow cytometry, the antibody reacts with the heavy chain of mouse IgG1. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgM, IgG2a, IgG2b, IgG3, and IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications. Results with your primary antibody may vary and specificity should be confirmed prior to performing two-color detection.

通过对与琼脂糖共价连接的小鼠 IgG1 进行亲和层析来分离特异性抗体。 基于 ELISA 和流式细胞术，抗体与小鼠 IgG1 的重链发生反应。 该抗体已通过斑点印迹和/或固相吸附测试，以确保与小鼠 IgM、IgG2a、IgG2b、IgG3 和 IgA、合并的人血清和纯化的人副蛋白的交叉反应最小。 该偶联物经过专门测试，可用于蛋白质印迹应用。 一抗的结果可能会有所不同，在进行双色检测之前应确认特异性。

Applications

Recommended for:

  • Western Blot
  • Protein Array
  • Immunohistochemistry
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging
  • 蛋白质印迹
    蛋白质阵列
    免疫组织化学
    显微镜
    2D 凝胶检测
    组织切片成像

Not Recommended for:

  • Small Animal Imaging
  • In-Cell Western Assay
  • On-Cell Western Assay

Formulation

IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 680LT 二抗以纯化的免疫球蛋白偶联物形式提供，在磷酸盐缓冲盐水中冻干，pH 7.4。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:20,000 1:20,000 – 1:50,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

Note: When using PVDF membranes for Western blotting applications, SDS (final concentration of 0.01 – 0.02%) and Tween® 20 (final concentration of 0.1 – 0.2%) must be added during the detection incubation step to avoid non-specific background staining.
注意：当使用 PVDF 膜进行蛋白质印迹应用时，必须在检测孵育步骤中添加 SDS（终浓度为 0.01 – 0.02%）和 Tween® 20（终浓度为 0.1 – 0.2%）以避免非特异性背景染色。

RRID

  • P/N 926-68050: RRID AB_2783642

Example Data

IRDye® 680LT Goat anti-Mouse IgG1-Specific Secondary Antibody
Western blot analysis of PTEN expression in mouse PTEN transfected 293T whole cell lysate (lane 2) and non-transfected 293T lysate (lane 3). Both lysates were loaded with 2 µg total protein per lane. Odyssey® One-Color Molecular Weight Marker (P/N 928-40000) was loaded in lane 1. The blot was probed with mouse ant-PTEN (IgG2b) (Santa Cruz P/N sc-133197) and mouse anti-GAPDH (IgG1) (Abcam P/N ab8245) followed by detection with IRDye 800CW Goat anti-Mouse IgG2b (P/N 926-32352) and IRDye 680LT Goat anti-Mouse IgG1 (926-68050)