标签：IRDye® 650 蛋白质标记试剂盒

Visible fluorescence protein labeling kits can be used to label antibodies and other proteins for applications such as flow cytometry, microscopy, immunohistochemistry, and other applications where fluorophore-conjugated antibodies are required.

The visible dyes included in these kits are activated with N-hydroxysuccinimide (NHS) esters, which is the most commonly used reactive group for labeling proteins. NHS esters react with primary amines, forming stable, covalent bonds.

VRDye™ labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Raise the pH of the preservative-free protein solution with the provided buffer, combine it with the appropriate amount of water-soluble amine-reactive dye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.

可见荧光蛋白标记试剂盒可用于标记抗体和其他蛋白，用于流式细胞术、显微镜检查、免疫组织化学和其他需要荧光团偶联抗体的应用。

这些试剂盒中包含的可见染料由 N-羟基琥珀酰亚胺 (NHS) 酯激活，这是标记蛋白质最常用的反应基团。 NHS 酯与伯胺反应，形成稳定的共价键。

VRDye™ 标记试剂盒基于一个简单的偶联方案，该方案使用快速旋转柱净化，其纯度与透析纯化相当。 使用提供的缓冲液提高不含防腐剂的蛋白质溶液的 pH 值，将其与适量的水溶性胺活性染料混合，然后使用随附的离心柱将标记的偶联物与游离染料分离。 标记和纯化在大约 2 小时内完成。

Properties of Visible Fluorescence NHS Ester Dyes

*CF is a correction factor for the absorbance of the dye at Abs_max to the absorbance of the protein at 280 nm.