Reagents > COX IV Rabbit Primary Antibody for Normalization
Cytochrome c oxidase is localized to the inner mitochondrial membrane. The COX IV antibody can be used as a mitochondrial loading control and an internal loading control (ILC) and is particularly effective when normalizing low-expressing target proteins.
The expression of COX IV, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.
Once validated, COX IV primary antibodies can be used for the detection of COX IV when performing two-color detection.
Detect COX IV Rabbit Monoclonal Primary Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.
Other options for housekeeping protein normalization
细胞色素 c 氧化酶定位于线粒体内膜。 COX IV 抗体可用作线粒体上样对照和内部上样对照 (ILC)，在标准化低表达靶蛋白时特别有效。
应验证 COX IV 或任何管家蛋白 (HKP) 的表达，以确保其表达在实验条件下不会改变。
一旦通过验证，COX IV 一抗可用于在进行双色检测时检测 COX IV。
使用 IRDye® 山羊抗兔或 IRDye 驴抗兔二抗检测 COX IV 兔单克隆一抗。
管家蛋白质标准化的其他选择
Reactivity and Specificity
COX IV antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.
Do not aliquot the antibody.
COX IV 抗体以 10 mM HEPES (pH 7.5)、150 mM NaCl、100 µg/mL BSA、50% 甘油和 <0.02% 叠氮化钠的形式提供。
不要等分抗体。
| Properties | COX IV Rabbit Monoclonal Antibody (P/N 926-42214) |
|---|---|
| Species Cross-Reactivity | Human, rabbit, monkey, zebrafish, bovine, pig |
| Target Molecular Weight | 17 kDa |
| Isotype | Rabbit IgG |
| Specificity/Sensitivity | Detects endogenous levels of total COX IV protein. |
| Immunogen | A synthetic peptide that corresponds to the residues surrounding Lys29 of human COX IV |
| Tested Applications | Western blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)
蛋白质印迹 (WB)、免疫沉淀 (IP)、免疫组织化学 (IHC)、免疫荧光 (IF)、流式细胞术 (F) |
RRID
- P/N 926-42214: RRID AB_2783000
COX IV Antibody Detected in Various Cell Lines
