标签：IRDye 驴抗兔二抗

IRDye® 山羊抗兔或 IRDye 驴抗兔二抗检测 GAPDH 兔单克隆抗体

Reagents > GAPDH Rabbit Monoclonal Antibody for Normalization

GAPDH Rabbit Monoclonal Antibody for Normalization

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a constitutively expressed housekeeping protein (HKP). The GAPDH primary antibody can be used as an internal loading control for normalization.

The expression of GAPDH, or any HKP, should be validated to ensure that its expression does not change under experimental conditions.

Once validated, GAPDH primary antibodies can be used for the detection of GAPDH when performing multiplex Western blot detection.

Detect GAPDH Rabbit Monoclonal Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.

3-磷酸甘油醛脱氢酶 (GAPDH) 是一种组成型表达的管家蛋白 (HKP)。 GAPDH 一抗可用作标准化的内部上样对照。

应验证 GAPDH 或任何 HKP 的表达，以确保其表达在实验条件下不会改变。

一旦经过验证，GAPDH 一抗可用于在进行多重蛋白质印迹检测时检测 GAPDH。

使用 IRDye® 山羊抗兔或 IRDye 驴抗兔二抗检测 GAPDH 兔单克隆抗体。

Other options for housekeeping protein normalization

Reactivity and Specificity

GAPDH antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

Properties GAPDH Rabbit Monoclonal Antibody (P/N 926-42216)
Species Cross-Reactivity Human, mouse, rat, monkey
Target Molecular Weight 37 kDa
Isotype Rabbit IgG
Specificity/Sensitivity Detects endogenous levels of total GAPDH protein. May cross-react with pig
Immunogen A synthetic peptide that corresponds to residues near the carboxy terminus of human GAPDH
Tested Applications Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

RRID

  • P/N 926-42216: RRID AB_2814901

GAPDH Rabbit Monoclonal Antibody in HeLa, NIH/3T3, and COS-7 Lysates

GAPDH Rabbit Monoclonal Antibody for Normalization
GAPDH Rabbit Monoclonal Antibody detected in HeLa, NIH/3T3, and COS-7 lysates. Lysates were diluted from 2.5 μg to 156 ng. Lysates were separated on 4-12% Bis-Tris gels, electrophoresed at 200V for 45 minutes in MES Running Buffer, and transferred to nitrocellulose membranes in Tris-Glycine buffer at 100V for 65 minutes. Blots were blocked with Intercept® (PBS) Protein-Free Blocking Buffer (P/N 927-90001), probed with GAPDH Rabbit Monoclonal Antibody, and detected on an Odyssey® CLx Imager.

IRDye® 山羊抗兔或 IRDye 驴抗兔二抗检测组蛋白 H3 兔单克隆抗体

Reagents > Histone H3 Rabbit Monoclonal Antibody for Normalization

Histone H3 Rabbit Monoclonal Antibody for Normalization

The Histone H3 primary antibody can be used as an internal loading control for normalization and is particularly effective when detecting target proteins in nuclear extracts.

The expression of Histone H3, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.

Once validated, Histone H3 primary antibodies can be used for the detection of Histone H3 when performing multiplex Western blot detection.

Detect Histone H3 Rabbit Monoclonal Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.

组蛋白 H3 一抗可用作标准化的内部上样对照，在检测核提取物中的靶蛋白时特别有效。

应验证组蛋白 H3 或任何管家蛋白 (HKP) 的表达，以确保其表达在实验条件下不会改变。

经验证后，在进行多重蛋白质印迹检测时，组蛋白 H3 一抗可用于检测组蛋白 H3。

使用 IRDye® 山羊抗兔或 IRDye 驴抗兔二抗检测组蛋白 H3 兔单克隆抗体。

Other options for housekeeping protein normalization

Reactivity and Specificity

Histone H3 antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

Properties Histone H3 Rabbit Monoclonal Antibody (P/N 926-42219)
Species Cross-Reactivity Human, mouse, rat, monkey
Target Molecular Weight 17 kDa
Isotype Rabbit IgG
Specificity/Sensitivity Detects endogenous levels of total Histone H3 protein (including isoforms H3.1, H3.2, and H3.3) and Histone H3 variant CENP-A. Does not cross-react with other core histones. May cross-react with bovine, chicken, D. melanogaster, hamster, xenopus, and zebrafish.
Immunogen A synthetic peptide that corresponds to the carboxy terminus of the human histone H3 protein
Tested Applications Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)

蛋白质印迹 (WB)、免疫组织化学 (IHC)、免疫荧光 (IF)、流式细胞术 (F)

RRID

  • P/N 926-42219: RRID AB_2814902

Detection of Histone H3 Rabbit Monoclonal Antibody in HeLa, NIH/3T3, and Cos7 Lysates

Histone H3 Rabbit Monoclonal Antibody for Normalization
Histone H3 Rabbit Monoclonal Antibody detected in HeLa, NIH/3T3, and COS-7 Lysates. Lysates were diluted from 2.5 μg to 156 ng. Lysates were separated on 4-12% Bis-Tris gels, electrophoresed at 200V for 45 minutes in MES Running Buffer, and transferred to nitrocellulose membranes in Tris-Glycine buffer at 100V for 65 minutes. Blots were blocked with Intercept® (PBS) Protein-Free Blocking Buffer (P/N 927-90001), probed with Histone H3 Rabbit Monoclonal Antibody, and detected on an Odyssey® CLx Imager.
在 HeLa、NIH/3T3 和 COS-7 裂解物中检测到组蛋白 H3 兔单克隆抗体。 裂解物从 2.5 μg 稀释至 156 ng。 在 4-12% Bis-Tris 凝胶上分离裂解物，在 MES Running Buffer 中以 200V 电泳 45 分钟，然后在 100V 的 Tris-Glycine 缓冲液中转移至硝酸纤维素膜 65 分钟。 印迹用 Intercept® (PBS) 无蛋白封闭缓冲液（P/N 927-90001）封闭，用组蛋白 H3 兔单克隆抗体探测，并在 Odyssey® CLx 成像仪上检测。

LI-COR IRDye® 山羊抗兔,IRDye 驴抗兔二抗,检测 β-微管蛋白兔多克隆抗体

Reagents > β-Tubulin Rabbit Polyclonal Antibody for Normalization,用于标准化的 β-微管蛋白兔多克隆抗体

β-Tubulin Rabbit Polyclonal Antibody for Normalization

The β-Tubulin primary antibody can be used as an internal loading control (ILC) for normalization.

The expression of β-tubulin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.

Once validated, β-tubulin primary antibodies can be used for the detection of β-tubulin when performing two-color detection.

Detect β-Tubulin Rabbit Polyclonal Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.

Other options for housekeeping protein normalization

β-微管蛋白一抗可用作内部上样对照 (ILC) 以进行标准化。

应验证 β-微管蛋白或任何管家蛋白 (HKP) 的表达，以确保其表达在实验条件下不会改变。

经验证后，β-微管蛋白一抗可在进行双色检测时用于检测 β-微管蛋白。

使用 IRDye® 山羊抗兔或 IRDye 驴抗兔二抗检测 β-微管蛋白兔多克隆抗体。

管家蛋白质标准化的其他选择

Reactivity and Specificity

β-Tubulin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

Properties β-Tubulin Rabbit Polyclonal Antibody (P/N 926-42211)
Species Cross-Reactivity Human, mouse, rat, monkey, bovine
Target Molecular Weight 55 kDa
Isotype Rabbit IgG
Specificity/Sensitivity Detects endogenous levels of total β-tubulin protein. Does not cross-react with recombinant α-tubulin.
Immunogen A synthetic peptide (KLH-coupled) that corresponds to the sequence of total β-tubulin, and does not cross-react with recombinant α-tubulin
Tested Applications Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)

蛋白质印迹 (WB)、免疫组织化学 (IHC)、免疫荧光 (IF)、流式细胞术 (F)

RRID

  • P/N 926-42211: RRID AB_1850029

β-Tubulin Detected in Various Cell Lines

β-Tubulin Rabbit Polyclonal Antibody for Normalization
β-Tubulin detected with β-Tubulin primary antibody (P/N 926-42211) in HeLa, NIH/3T3, and COS7 cell lysates.