标签：IRDye 荧光染料

IRDye Infrared Fluorescent Dyes and near-infrared (NIR) fluorescence imaging deliver enhanced sensitivity due to low background autofluorescence in the near-infrared region and, therefore, higher signal-to-noise ratios.

IRDye Fluorescent Dyes have absorption and emission wavelengths in the NIR spectrum, between 680 and 800 nm. Most of the IRDye reagents are compatible with the Odyssey^® DLx, Odyssey XF, Odyssey Sa, Pearl^® Imagers, and 4300 DNA Analyzer platforms.

IRDye 红外荧光染料和近红外 (NIR) 荧光成像由于在近红外区域的背景自发荧光低，因此具有更高的信噪比，从而提高了灵敏度。

IRDye 荧光染料在 NIR 光谱中具有 680 至 800 nm 的吸收和发射波长。 大多数 IRDye 试剂与 Odyssey® DLx、Odyssey XF、Odyssey Sa、Pearl® Imagers 和 4300 DNA 分析仪平台兼容。

NHS Esters

Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye Infrared Dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amino groups.

Maleimides

IRDye maleimides provide the functionality for labeling molecules that contain free sulfhydryl (-SH) groups with IRDye Infrared Dyes. The reactive group of the IRDye maleimide allows conjugation reactions to be performed very efficiently at physiological pH.

IRDye 马来酰亚胺提供了用 IRDye 红外染料标记含有游离巯基 (-SH) 基团的分子的功能。 IRDye 马来酰亚胺的反应性基团允许偶联反应在生理 pH 值下非常有效地进行。

Carboxylates

Assays that use infrared dye conjugates (such as in vivo imaging and cell binding assays) may require a “dye-only” control for potential effects or retention of the dye. The carboxylate dye has no reactive group and cannot be used for labeling.

Carboxylate dye forms can serve as an ideal control for cell-based assays that monitor binding of a dye-labeled agent to validate optical agents for in vivo administration and to evaluate binding specificity. This dye form can also be used to evaluate the behavior and clearance of the dye itself and as a standard to determine the amount of unreacted (“free”) dye after conjugation and purification.

使用红外染料偶联物的测定（例如体内成像和细胞结合测定）可能需要“仅染料”控制以控制染料的潜在影响或保留。 羧酸染料没有反应基团，不能用于标记。

羧酸盐染料形式可以作为基于细胞的测定的理想对照，监测染料标记试剂的结合，以验证用于体内给药的光学试剂并评估结合特异性。 这种染料形式还可用于评估染料本身的行为和清除率，并作为确定结合和纯化后未反应（“游离”）染料量的标准。

Labeling Kits

IRDye Protein Labeling Kits label antibodies, other proteins, and peptides for applications such as Western blots, In Cell Western™ assays, in vivo imaging, and whole organ or tissue section assays. Applications of dye-labeled conjugates vary, depending on the IRDye used for labeling.

IRDye Labeling Kits

DBCO, Alkyne, and Azide Dye Forms Used for Click Chemistry

Biomolecule labeling continues to be a cornerstone feature of many in vitro and in vivo biological experiments. Click Chemistry has recently emerged as a convenient, versatile, and reliable method for labeling a wide variety of molecules for applications ranging from biomarker isolation to assay development. Click Chemistry utilizes pairs of reagents that exclusively react with each other and are effectively inert to naturally occurring functional groups such as amines

LI-COR offers DBCO, azide, and alkyne dye forms of IRDye 800CW and IRDye 680RD.

Click Chemistry Dyes

IRDye Properties and Forms Available

Dye	Exmax (nm)*	Emmax (nm)*	NHS Ester	Maleimide	Carboxylate	DBCO	Azide	Alkyne	Phosphoramidite	LI-COR Channel
IRDye 800CW	778	794								800 nm
IRDye 800RS	770	786								800 nm
IRDye 800 phosphoramidite	787	812								800 nm
IRDye 750	766	776
IRDye 700 phosphoramidite	680	697								700 nm
IRDye 680LT**	680	694								700 nm
IRDye 680RD	680	694								700 nm

Which 700 Channel Dye Should I Use?

LI-COR offers three distinct 700 channel dyes for a variety of applications. Choosing the correct dye is critical when developing an experiment. A summary of each dye and an application table are provided to help you determine which dye is best for your needs.

IRDye 680RD

IRDye 680RD is the near-infrared fluorescent dye of choice for small animal imaging, Western blot, and In-Cell Western Assay applications in the 700nm channel. It has the lowest background compared to LI-COR’s other 700 nm dyes and can be used for the widest variety of applications with little optimization. The NHS ester and maleimide forms of this infrared dye are ideal for labeling proteins, peptides, and antibodies. LI-COR offers a variety of IRDye 680RD dyes, labeling kits and secondary antibodies.

IRDye 680LT

IRDye 680LT is significantly brighter and more photostable than many other 700 nm near-infrared dyes such as Alexa Fluor^® 680. It provides superior performance for protein detection applications, including microscopy and Western blot applications. LI-COR offers a variety of IRDye 680LT dyes, labeling kits, and secondary antibodies. When using IRDye 680LT secondary antibodies for Western blot detection, it is critical to follow the recommendations in the pack insert. Dilution optimization may be required to achieve best results.

Application	IRDye 680RD	IRDye 680LT
Western Blot
In-Cell Western Assay		not recommended
On-Cell Western Assay		not recommended
RNAi Screens		for Western blots only
Protein Array
Immunohistochemistry
Microscopy
2D Gel Detection
Tissue Section Imaging
FRET-based Assays
Small Animal Imaging		not recommended

IRDye Infrared Dyes are Optimized for Near-Infrared Fluorescent Detection

IRDye Near-Infrared Dyes are optimized for excitation and detection in the near-infrared spectrum, providing numerous benefits.

• Low background autofluorescence allows higher signal to noise ratios (see Figure 1)
• Wide range of dyes and functional groups available for multiple applications
• Excellent photostability
• Dye brightness

IRDye 800CW Infrared Dye Successfully Completes Toxicity Studies

LI-COR offers IRDye 800CW that has been manufactured under current good manufacturing practices (cGMP) and is available for conjugation to targeted biomolecules for investigational use in clinical trials. In September of 2007, LI-COR Biosciences announced the successful completion of animal toxicity studies of its IRDye 800CW infrared dye carboxylic acid using a protocol reviewed by the Food and Drug Administration. The completion of the toxicity studies was a key milestone in the development of the IRDye 800CW dye for potential clinical imaging use. The complete report was published in 2010 and is available to the public. In addition, drug master files (DMF) are on record with U.S. and European regulatory authorities.

LI-COR 提供的 IRDye 800CW 是根据当前的良好生产规范 (cGMP) 制造的，可用于与靶向生物分子结合，用于临床试验的研究用途。 2007 年 9 月，LI-COR Biosciences 宣布使用食品和药物管理局审查的协议成功完成了 IRDye 800CW 红外染料羧酸的动物毒性研究。 毒性研究的完成是 IRDye 800CW 染料开发用于潜在临床成像的关键里程碑。 完整的报告于 2010 年发布，可供公众查阅。 此外，药物主文件 (DMF) 已在美国和欧洲监管机构备案。

LI-COR IRDye Infrared Fluorescent Dyes are used for protein and cellular assays, microscopy, and in vivo molecular imaging in animals for research purposes. IRDye 800CW is cited in a wide variety of published non-clinical research for labeling nucleic acids, antibodies, proteins, and peptides where there is a need for high signal, low background imaging. With an 800nm emission wavelength, the IRDye 800CW dye is spectrally ideal for animal imaging research.

While the IRDye 800CW dye has successfully completed toxicity studies, it has not been studied for diagnostic or therapeutic use in humans, and has not been approved by the Food and Drug Administration for this use. LI-COR is exploring options for additional studies as next steps in the Food and Drug Administration approval process leading toward clinical use.

LI-COR IRDye 红外荧光染料用于研究目的的动物蛋白质和细胞分析、显微镜检查和体内分子成像。 IRDye 800CW 在各种已发表的非临床研究中被引用，用于标记需要高信号、低背景成像的核酸、抗体、蛋白质和肽。 IRDye 800CW 染料的发射波长为 800nm，是动物成像研究的理想光谱。

虽然 IRDye 800CW 染料已成功完成毒性研究，但尚未研究用于人类的诊断或治疗用途，也未获得美国食品和药物管理局的批准用于此用途。 LI-COR 正在探索其他研究的选择，作为食品和药物管理局批准程序的下一步，以实现临床使用。

LI-COR IRDye Infrared Dyes are Being Used in Clinical Trials

IRDye 800CW conjugated probes and agents have propelled more than 20 Phase I and Phase II clinical trials, To learn more about LI-COR infrared dye conjugates being used in clinical trials, visit Probe Development Clinical Applications.