标签：Intercept® (TBS) Blocking Buffer

Intercept® (TBS) Blocking Buffer,(TBS) 封闭缓冲液

Reagents > Intercept® (TBS) Blocking Buffer

Intercept® (TBS) Blocking Buffer

Intercept (TBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS).

  • Contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies.
  • Can be used with the widest range of targets for Western blot detection.
  • Ideal for primary antibodies that are optimized for Tris-based buffer systems.
  • Can be used for detection of phosphorylated targets.
  • Has superior performance for alkaline phosphatase detection.
  • Excellent choice for biotin-streptavidin detection.

For larger pack sizes, please contact your local sales office.

Intercept (TBS) 封闭缓冲液经过配制，可为可复制的定量蛋白质印迹和其他免疫测定提供高效封闭和低背景变异性。 Intercept (TBS) 封闭缓冲液是一种在 Tris 缓冲盐水 (TBS) 中的即用型封闭剂配方。

不含哺乳动物蛋白，与哺乳动物抗体的交叉反应性最低。
可用于最广泛的蛋白质印迹检测目标。
非常适合针对基于 Tris 的缓冲系统进行优化的一抗。
可用于检测磷酸化目标。
具有卓越的碱性磷酸酶检测性能。
生物素-链霉亲和素检测的绝佳选择。
如需更大的包装尺寸，请联系您当地的销售办事处。

Formulation

Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in TBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon®-FL PVDF and nitrocellulose membranes.

Intercept (TBS) 封闭缓冲液是 TBS 中的即用型封闭剂配方。 它不含叠氮化钠，因此适用于近红外或化学发光检测。 它在 4 °C 下储存，也可用于包含 Immobilon®-FL PVDF 和硝酸纤维素膜的试剂盒中。

Shake well before each use.

Intercept® (TBS) Blocking Buffer
Figure 1: Western blot protocol demonstrating the comparison of Intercept Blocking Buffer to Odyssey Blocking Buffer. Blots with serial dilution of mouse heart tissue extract were probed for Stat3 Mouse mAb and p38 MAPK Rabbit Ab, detected with IRDye® 800CW Goat anti-Mouse IgG (P/N 925-32210) and IRDye 680LT Goat anti-Rabbit IgG (P/N 925-68021), and scanned together on an Odyssey CLx Imaging System.
图 1：Western 印迹协议，展示了 Intercept Blocking Buffer 与 Odyssey Blocking Buffer 的比较。 使用 IRDye® 800CW 山羊抗小鼠 IgG（部件号 925-32210）和 IRDye 680LT 山羊抗兔 IgG（部件号/ N 925-68021)，并在 Odyssey CLx 成像系统上一起扫描。

Intercept (TBS) Blocking Buffer can be used for many immunoassays and applications, including:

  • Quantitative Western Blot
  • Chemiluminescent Western Blots
  • In-Cell Western™ Assay
  • In-Gel Western
  • Protein Array
  • Glycoprotein Detection
  • 定量蛋白质印迹
    化学发光蛋白质印迹
    In-Cell Western™ 检测
    凝胶西部
    蛋白质阵列
    糖蛋白检测

Blocking Buffers,Intercept® (TBS) Blocking Buffer,Intercept® (TBS) 封闭缓冲液

Blocking Buffers

Intercept® (TBS) Blocking Buffer,Intercept® (TBS) 封闭缓冲液

Blocking Buffers

Intercept® (PBS) Blocking Buffer,Intercept® (PBS) 封闭缓冲液

Blocking Buffers

Intercept® (TBS) Protein-Free Blocking Buffer,Intercept® (TBS) 无蛋白封闭缓冲液

Blocking Buffers

Intercept® (PBS) Protein-Free Blocking Buffer,Intercept® (PBS) 无蛋白封闭缓冲液

Blocking Buffers

Intercept® (TBS) Blocking Buffer and Diluent Kit,Intercept® (TBS) 封闭缓冲液和稀释剂试剂盒

Blocking Buffers

Intercept® (PBS) Blocking Buffer and Diluent Kit,Intercept® (PBS) 封闭缓冲液和稀释剂试剂盒

Blocking Buffers

Figure 1: Western blot protocol demonstrating the comparison of Intercept Blocking Buffer to Odyssey Blocking Buffer. Blots with serial dilution of mouse heart tissue extract were probed for Stat3 Mouse mAb and p38 MAPK Rabbit Ab, detected with IRDye 800CW Goat anti-Mouse IgG and IRDye 680LT Goat anti-Rabbit IgG, and scanned together on an Odyssey CLx Imaging System.

图 1：Western 印迹协议，展示了 Intercept Blocking Buffer 与 Odyssey Blocking Buffer 的比较。 用连续稀释的小鼠心脏组织提取物检测 Stat3 Mouse mAb 和 p38 MAPK Rabbit Ab，用 IRDye 800CW 山羊抗小鼠 IgG 和 IRDye 680LT 山羊抗兔 IgG 检测，并在 Odyssey CLx 成像系统上一起扫描。

LI-COR,GAM/GAR CellTag 520 Stain ICW 细胞In-Cell Western™ 检测试剂盒

Reagents > GAM/GAR CellTag™ 520 Stain ICW Kit III

GAM/GAR CellTag™ 520 Stain ICW Kit III

GAM/GAR CellTag 520 Stain ICW Kits provide detection reagents for cell-based In-Cell Western™ Assays. This cost-effective normalization method makes quantification of the target protein more precise. CellTag 520 Stain accumulates in the nucleus and cytoplasm of permeabilized cells allowing for accurate normalization to cell number.

This kit includes reagents to perform an In-Cell Western Assay and CellTag 520 Stain to normalize well-to-well variations in cell number.

GAM/GAR CellTag 520 Stain ICW 试剂盒为基于细胞的 In-Cell Western™ 检测提供检测试剂。 这种具有成本效益的标准化方法使目标蛋白的定量更加精确。 CellTag 520 Stain 积聚在透化细胞的细胞核和细胞质中，可准确归一化细胞数量。

该试剂盒包括用于进行 In-Cell Western 检测的试剂和 CellTag 520 Stain 以标准化细胞数量的孔间差异。

In-Cell Western Assay Overview

Kit Components

  • IRDye® 800CW Goat anti-Mouse Secondary Antibody, 0.5 mg
  • IRDye 680RD Goat anti-Rabbit Secondary Antibody, 0.5 mg
  • Intercept® (TBS) Blocking Buffer, 1 x 500 mL
  • CellTag 520 Stain, 2 x 50 nmol
  • Kit Protocol

LI-COR,CellTag 700 染色剂,GAR CellTag 700 Stain ICW 细胞In-Cell Western™ 试剂盒

Reagents > GAR CellTag™ 700 Stain ICW Kit II

GAR CellTag™ 700 Stain ICW Kit II

GAR CellTag 700 Stain ICW Kits provide detection reagents for cell-based In-Cell Western™ Assays. This cost-effective normalization method makes quantification of the target protein more precise. CellTag 700 Stain accumulates in the nucleus and cytoplasm of permeabilized cells allowing for accurate normalization to cell number.

This kit includes reagents to perform an In-Cell Western assay and CellTag 700 Stain to normalize well-to-well variations in cell number. CellTag 700 accumulates in the nucleus and cytoplasm of permeabilized cells allowing for accurate normalization to cell number.

GAR CellTag 700 Stain ICW 试剂盒为基于细胞的 In-Cell Western™ 检测提供检测试剂。 这种具有成本效益的标准化方法使目标蛋白的定量更加精确。 CellTag 700 Stain 积聚在透化细胞的细胞核和细胞质中，可准确归一化细胞数量。

该试剂盒包括用于进行 In-Cell Western 检测的试剂和用于标准化细胞数量的孔间差异的 CellTag 700 Stain。 CellTag 700 积聚在透化细胞的细胞核和细胞质中，可准确标准化细胞数量。

In-Cell Western Assay Overview

Kit Components

  • IRDye 800CW Goat anti-Rabbit Secondary Antibody, 0.5 mg
  • Intercept® (TBS) Blocking Buffer, 1 x 500 mL
  • CellTag 700 Stain, 2 x 10 nmol
  • Kit Protocol

LI-COR P/N 926-32099,P/N 926-32100,TBS 封闭缓冲液和 PVDF 试剂盒

Reagents > TBS Blocking Buffer and PVDF Kits,TBS 封闭缓冲液和 PVDF 试剂盒

TBS Blocking Buffer and PVDF Kits

The non-mammalian Intercept® Blocker in these kits is in TBS (Tris-buffered saline) containing 0.06% Kathon II. The Immobilon®-FL PVDF membrane included in these kits has been tested to ensure a low background for infrared Western blot success.

这些试剂盒中的非哺乳动物 Intercept® Blocker 采用含有 0.06% Kathon II 的 TBS（Tris 缓冲盐水）。 这些试剂盒中包含的 Immobilon®-FL PVDF 膜已经过测试，以确保红外蛋白质印迹成功的低背景。

Kit Components

The Single Buffer Kit (P/N 926-32099) contains:

  • Intercept (TBS) Blocking Buffer, 500 mL
  • Millipore Immobilon-FL PVDF Membrane (1 roll; 0.45µm, 26.5 cm x 3.75 m)

The Bulk Buffer Kit (P/N 926-32100) contains:

  • Intercept (TBS) Blocking Buffer, 10 x 500 mL
  • Millipore Immobilon FL PVDF Membrane (1 roll, 0.45 µm; 26.5 cm x 3.75 m)