含有 ≥ 800,000 个细胞的肠上皮细胞 (InEpC) 冻存安瓿
Product Overview
InEpC’s are truly primary cells representing both villi (enterocytes, goblet, and enteroendocrine cells) and crypts structures. In combination with human intestinal myofibroblasts (InMyoFib), InEpC are able to form very tight cell monolayer, representing a unique in vitro system to model human intestinal homeostasis.
Human intestinal epithelial cells (InEpC) are of pivotal importance in the digestion and absorption of nutrients and the protection of the organism from bacteria and toxins found in the intestine. The intestinal epithelium is a highly organized cellular system maintained in a dynamic steady state by cells in proliferation and differentiation, which die and exfoliate into the lumen. Intestinal cells are often difficult to isolate due to contamination issues and usually have to be cultured and used for assays immediately after isolation from tissue.
Cryopreserved InEpCs are sold at P0, express cytokeratins 8/18 at ≥ 90%, contain 0.8M viable cells / vial and have a seeding efficiency ≥ 50% coverage at certain cell density (see instructions). Their life span is, post plating, that the cells can be used on days 5-7, and they maintain their properties for approximately 5 days after that (using CloneticsTM reagents & protocol). All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
InEpC 是真正的原代细胞，代表绒毛（肠细胞、杯状细胞和肠内分泌细胞）和隐窝结构。与人类肠道肌成纤维细胞 (InMyoFib) 结合，InEpC 能够形成非常紧密的细胞单层，代表了一种独特的体外系统来模拟人类肠道稳态。
人类肠道上皮细胞 (InEpC) 在营养物质的消化和吸收以及保护生物体免受肠道中发现的细菌和毒素的侵害方面至关重要。肠上皮是一种高度组织化的细胞系统，由增殖和分化中的细胞维持在动态稳定状态，这些细胞死亡并脱落进入管腔。由于污染问题，肠细胞通常难以分离，并且通常必须在从组织中分离后立即进行培养和用于检测。
冷冻保存的 InEpC 以 P0 销售，以 ≥ 90% 表达细胞角蛋白 8/18，包含 0.8M 活细胞/小瓶，并且在一定细胞密度下具有 ≥ 50% 的接种效率（见说明）。它们的寿命是，在铺板后，细胞可以在第 5-7 天使用，并且在此之后它们保持其特性大约 5 天（使用 CloneticsTM 试剂和方案）。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。
Content & Storage
Content
1 x Cryopreserved ampule with ≥ 800,000 cells
InMyoFib – 人类肠道肌成纤维细胞 CC-2902
人肠上皮和肌成纤维细胞组合 CC-4540
RPTEC – 人肾近端小管上皮细胞 CC-2553
