标签：In-Cell Western™ 检测

Intercept® (TBS) 封闭缓冲液和稀释剂试剂盒,In-Cell Western™ 检测

Reagents > Intercept® (TBS) Blocking Buffer and Diluent Kit,Intercept® (TBS) 封闭缓冲液和稀释剂试剂盒

Intercept® (TBS) Blocking Buffer and Diluent Kit

Try Intercept (TBS) Blocking Buffer and Intercept T20 (TBS) Antibody Diluent for improved results in your quantitative Western blots and other immunoassays. Intercept Blocking Buffers provide excellent blocking performance with low background and low variability.

Intercept T20 Antibody Diluents improve the specificity of the primary and secondary antibodies, reducing off-target effects. They are preformulated with Tween® 20. There’s no need to mix the diluent yourself, which saves you time and reduces potential variation.

尝试使用 Intercept (TBS) 封闭缓冲液和 Intercept T20 (TBS) 抗体稀释剂来改善定量蛋白质印迹和其他免疫测定的结果。 Intercept Blocking Buffers 提供出色的封闭性能，具有低背景和低变异性。

Intercept T20 Antibody Diluents 提高一抗和二抗的特异性，减少脱靶效应。 它们是用 Tween® 20 预先配制的。无需自己混合稀释剂，这样可以节省您的时间并减少潜在的变化。

Kit Components

  • Intercept (TBS) Blocking Buffer, 1 x 500 mL
  • Intercept T20 (TBS) Antibody Diluent, 2 x 500 mL
  • 截留 (TBS) 封闭缓冲液，1 x 500 mL
    Intercept T20 (TBS) 抗体稀释剂，2 x 500 mL

Shake well before each use.

Intercept (TBS) Blocking Buffer and Intercept T20 (TBS) Antibody Diluent can be used for many immunoassays and applications, including:

  • Quantitative Western Blot
  • Chemiluminescent Western Blot
  • In-Cell Western™ Assay
  • In-Gel Western
  • Protein Array
  • Glycoprotein Detection
  • 定量蛋白质印迹
    化学发光免疫印迹
    In-Cell Western™ 检测
    凝胶西部
    蛋白质阵列
    糖蛋白检测

LI-COR P/N 925-68180,In-Cell Western™ 检测,IRDye® 680RD 山羊抗小鼠 IgM（μ 链特异性）二抗

Reagents > IRDye® 680RD Goat anti-Mouse IgM (µ chain specific) Secondary Antibody,IRDye® 680RD 山羊抗小鼠 IgM（μ 链特异性）二抗

IRDye® 680RD Goat anti-Mouse IgM (µ chain specific) Secondary Antibody

Immunogen

Mouse IgM paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgM covalently linked to agarose. Based on ELISA and/or flow cytometry, this antibody reacts with the heavy chain of mouse IgM. This antibody was tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgG1, IgG2a, IgG3, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications.

使用与琼脂糖共价连接的小鼠 IgM 通过亲和层析实现特异性抗体的分离。 基于 ELISA 和/或流式细胞术，该抗体与小鼠 IgM 的重链发生反应。 该抗体通过斑点印迹和/或固相吸附测试，以确保与小鼠 IgG1、IgG2a、IgG3 和 IgA、合并的人血清和纯化的人副蛋白的交叉反应最小。 该偶联物经过专门测试，可用于蛋白质印迹应用。

Applications

Highly recommended for:

  • Western Blot
  • In-Cell Western™ Assay
  • On-Cell Western Assay
  • Protein Array
  • Immunohistochemistry
  • Small Animal Imaging
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging
  • 蛋白质印迹
    In-Cell Western™ 检测
    On-Cell Western 检测
    蛋白质阵列
    免疫组织化学
    小动物成像
    显微镜
    2D 凝胶检测
    组织切片成像

Formulation

IRDye 680RD secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 680RD 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application Recommended Suggested Range
Odyssey® Western blot detection 1:15,000 1:5,000 – 1:25,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

RRID

  • P/N 925-68180: RRID AB_2814918
  • P/N 926-68180: RRID AB_2814921

LI-COR IRDye® 800CW 驴抗豚鼠 IgG 二抗,In-Cell Western™ 检测

Reagents > IRDye® 800CW Donkey anti-Guinea Pig IgG Secondary Antibody,IRDye® 800CW 驴抗豚鼠 IgG 二抗

IRDye® 800CW Donkey anti-Guinea Pig IgG Secondary Antibody

Immunogen

Guinea Pig IgG, whole molecule

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule guinea pig IgG as well as the light chains common to other guinea pig immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications.

使用固定在琼脂糖珠上的抗原通过亲和层析实现特异性抗体的分离。 基于免疫电泳和/或 ELISA，该抗体与全分子豚鼠 IgG 以及其他豚鼠免疫球蛋白共有的轻链反应。 未检测到针对非免疫球蛋白血清蛋白的反应性。 该抗体已通过 ELISA 和/或固相吸附测试，以确保与牛、鸡、山羊、叙利亚仓鼠、马、人、小鼠、兔、大鼠和绵羊血清蛋白的交叉反应最小。 该偶联物经过专门测试，可用于蛋白质印迹和 In-Cell Western™ 检测应用。

Applications

Highly recommended for:

  • Western Blot
  • In-Cell Western Assay
  • On-Cell Western Assay
  • Protein Array
  • Immunohistochemistry
  • Small Animal Imaging
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging
  • Virus Titration Assay
  • 蛋白质印迹
    细胞内西方检测
    On-Cell Western 检测
    蛋白质阵列
    免疫组织化学
    小动物成像
    显微镜
    2D 凝胶检测
    组织切片成像
    病毒滴定法

Formulation

IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 800CW 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:15,000 1:5,000 – 1:25,000
In-Cell Western assay detection 1:800 1:200 – 1:1,200
Other User optimized

Optimum dilutions will vary and should be determined empirically.

RRID

  • P/N 925-32411: RRID AB_2814905
  • P/N 926-32411: RRID AB_1850024

Example Data

IRDye® 800CW Donkey anti-Guinea Pig IgG Secondary Antibody
Detection of guinea pig IgG with IRDye 800CW Donkey anti-Guinea Pig. Serial dilutions of Guinea Pig IgG were spiked into C32 lysate (Santa Cruz P/N sc-2205). Samples were resolved on a 10% Bis-Tris gel and transferred to Odyssey® Nitrocellulose membrane (P/N 926-31092). The membrane was blocked with Odyssey Blocking Buffer (P/N 927-40000) followed by detection with IRDye 800CW Donkey anti-Guinea Pig (P/N 926-32411).