标签：HMVEC-dAd -人真皮微血管内皮细胞 -成人

lonza,CC-2543,HMVEC-dAd -人真皮微血管内皮细胞 -成人

Catalog #: CC-2543

Cryopreserved ampule of Adult Human Dermal Microvascular Endothelial Cells (HMVEC-d Ad) containing ≥ 500,000 cells

Product Overview

人真皮微血管内皮细胞包括从成人或新生儿包皮的皮肤组织内的小血管中分离出来的细胞。培养的细胞纯度≥90%，并专门富集真皮 LEC 和 BEC。来自新生儿或成人的真皮微血管内皮细胞 (HMVEC-D) 是血液 (HMVEC-DB1) 和淋巴 (HMVEC-DLy) 内皮细胞的混合群体。

成体细胞用“广告”表示；新生细胞用“Neo”表示。汇集和单一捐助者都可用。通过联系我们的科学支持团队，可以获得更多的捐赠者特征。内皮细胞和培养基使用方便且易于使用，使研究人员能够专注于结果。 Lonza 的细胞、培养基和试剂一起经过质量测试，并保证作为一个完整的细胞系统提供最佳性能。

冷冻保存的成人皮肤微血管内皮细胞在第三代运输。冷冻保存的真皮微血管内皮细胞保证通过 15 次群体倍增（CC-2543、CC-2505 和 CC-2516）和 12 次群体倍增（CC-2810、CC-2811、CC-2812 和 CC-2813），表达 CD31/105 ，von Williebrand 因子 VIII，对乙酰化低密度脂蛋白摄取呈阳性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

Benefits

  • Ready-To-Use
  • No tissue dissection required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Safety Data Sheets (SDS)

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Data
HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult

HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult

Human microvascular endothelial cells (CC-2543) were stained for von Willebrand Factor using antibody (Sigma, F3520) at 1:200 dilution (green). Secondary antibody used was VECTDI1088 DyLight 488 (Vector Laboratories). To detect CD31/PECAM-1, cells were stained with (R&D Systems, BBA7) at 1:100 dilution(red). Secondary antibody used was VECTDI2594 DyLight 594 (Vector Laboratories).

使用稀释度为 1:200（绿色）的抗体（Sigma，F3520）对人微血管内皮细胞（CC-2543）进行血管性血友病因子染色。 使用的二抗是 VECTDI1088 DyLight 488 (Vector Laboratories)。 为了检测 CD31/PECAM-1，细胞以 1:100 稀释度（红色）用（R&D Systems，BBA7）染色。 使用的二抗是 VECTDI2594 DyLight 594 (Vector Laboratories)。

HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult
HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult

HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult

Human microvascular endothelial cells (CC-2543) were stained for von Willebrand Factor using antibody (Sigma, F3520) at 1:200 dilution (green). Secondary antibody used was VECTDI1088 DyLight 488 (Vector Laboratories). To detect CD31/PECAM-1, cells were stained with (R&D Systems, BBA7) at 1:100 dilution(red). Secondary antibody used was VECTDI2594 DyLight 594 (Vector Laboratories).

使用稀释度为 1:200（绿色）的抗体（Sigma，F3520）对人微血管内皮细胞（CC-2543）进行血管性血友病因子染色。 使用的二抗是 VECTDI1088 DyLight 488 (Vector Laboratories)。 为了检测 CD31/PECAM-1，细胞以 1:100 稀释度（红色）用（R&D Systems，BBA7）染色。 使用的二抗是 VECTDI2594 DyLight 594 (Vector Laboratories)。