标签：hADSC 人类脂肪源性干细胞

Product Overview

PoieticsTM 正常人脂肪衍生干细胞 (hADSC) 是从在择期手术吸脂过程中收集的正常（非糖尿病）成人吸脂中分离出来的。

据报道，正常人 ADSC 可分化出许多不同的谱系，包括软骨形成、成骨、脂肪形成和神经。正常人 ADSC 在初次传代时已被冷冻保存。

相关的额外捐赠者信息，包括体重指数 (BMI) 可通过联系科学支持获得。冷冻保存的 PoieticsTM 正常人 ADSC 保证经过 5 次传代，表达 CD13、CD29、CD44、CD73、CD90、CD105 和 CD166，不表达 CD14、CD31 和 CD45。

所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

来自糖尿病捐赠者的人类 ADSC 作为单独的产品提供。这些细胞表现出与骨髓来源的间充质干细胞非常相似的表型和功能特征。

Recommended Media and Reagents

Growth Medium:

• ADSC Human Adipose Derived Stem Cell Growth BulletKit^TM Medium

Differentiation Medium*:

• Adipogenic: PGM^TM -2 Preadipocyte Growth Medium-2 BulletKit^TM Medium (provides both a plating medium and a differentiation medium appropriate to induce differentiation of human adipose derived mesenchymal stem cells into mature, functionally active adipocytes; Each bottle of the PGM-2^TM BulletKit^TM Medium contains enough differentiation medium to fully differentiate up to 20 million Adipose Derived Stem Cells (ADSC) [10 complete 96-well plates] into mature, functionally active adipocytes).

• Chondrogenic: hMSC Chondrogenic Differentiation BulletKit^TM Medium, with the addition of TGF-β3 (provides a differentiation medium appropriate to induce chondrogenic differentiation of human adipose derived mesenchymal stem cells into mature, functionally active chondrocytes; Each bottle of the hMSC Chondrogenic Differentiation BulletKit^TM Medium, with the addition of TGF-β3 (sold separately), contains enough differentiation medium to fully differentiate approximately 12.5 million human adipose derived mesenchymal stem cells [approximately 25 micropellets] into mature, functionally active chondrocytes).

• Osteogenic: hMSC Osteogenic Differentiation BulletKit^TM Medium (provides a differentiation medium appropriate to induce osteogenic differentiation of human adipose derived mesenchymal stem cells into mature, functionally active osteoblasts; Each bottle of the hMSC Osteogenic Differentiation BulletKit^TM Medium contains enough differentiation medium to fully differentiate approximately 200,000 human adipose derived mesenchymal stem cells [approximately 20 wells in a 24-well plate format] into mature, functionally active osteoblasts).

Lonza guarantees the performance of Clonetics^TM/Poietics^TM cells only if appropriate Clonetics^TM/Poietics^TM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

*Lonza does not routinely test differentiation of Lonza’s human adipose derived mesenchymal stem cells and cannot guarantee differentiation for all lots.

糖尿病 I 型和 II 型脂肪干细胞 (ADSC) PT-5008