Reagents > GAPDH Rabbit Monoclonal Antibody for Normalization
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a constitutively expressed housekeeping protein (HKP). The GAPDH primary antibody can be used as an internal loading control for normalization.
The expression of GAPDH, or any HKP, should be validated to ensure that its expression does not change under experimental conditions.
Once validated, GAPDH primary antibodies can be used for the detection of GAPDH when performing multiplex Western blot detection.
Detect GAPDH Rabbit Monoclonal Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.
3-磷酸甘油醛脱氢酶 (GAPDH) 是一种组成型表达的管家蛋白 (HKP)。 GAPDH 一抗可用作标准化的内部上样对照。
应验证 GAPDH 或任何 HKP 的表达，以确保其表达在实验条件下不会改变。
一旦经过验证，GAPDH 一抗可用于在进行多重蛋白质印迹检测时检测 GAPDH。
使用 IRDye® 山羊抗兔或 IRDye 驴抗兔二抗检测 GAPDH 兔单克隆抗体。
Other options for housekeeping protein normalization
Reactivity and Specificity
GAPDH antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.
Do not aliquot the antibody.
| Properties | GAPDH Rabbit Monoclonal Antibody (P/N 926-42216) |
|---|---|
| Species Cross-Reactivity | Human, mouse, rat, monkey |
| Target Molecular Weight | 37 kDa |
| Isotype | Rabbit IgG |
| Specificity/Sensitivity | Detects endogenous levels of total GAPDH protein. May cross-react with pig |
| Immunogen | A synthetic peptide that corresponds to residues near the carboxy terminus of human GAPDH |
| Tested Applications | Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF) |
RRID
- P/N 926-42216: RRID AB_2814901
GAPDH Rabbit Monoclonal Antibody in HeLa, NIH/3T3, and COS-7 Lysates
