标签：CTC 多种方式用于检测细菌

Dojindo,CTC/100/C440,CTC 多种方式用于检测细菌

根据目标，CTC 可以多种方式用于检测细菌。检测方法包括琼脂平板培养法，其中包括对细菌培养产生的菌落进行计数；使用荧光染色法对细菌进行染色；使用 DVC 方法检测具有生长潜力的细菌；使用FISH法-DNA扩增法检测特定细菌。 CTC 通过呼吸活动通过电子转移还原为 CTC 甲臜 (CTF)，并在不溶于水的细胞内以荧光沉降的形式积聚。 CTC本身是水溶性的，在水溶液中不发荧光；然而，CTF 在低粘度流体中不发荧光。然而，在高粘度和固态的流体中，它会发出红色荧光。通过在荧光显微镜下计数或通过流式细胞仪分析，可以在将 CTC 与试剂一起孵育后寻找具有呼吸活性的细胞。通过使用核酸染色试剂对总细胞群和活细胞数量进行计数，或者使用 FISH 方法对特定的活细胞类型进行选择性计数，可以收集更高水平的数据。由于 VNC（可存活但不可培养）细菌的存在（已变得明显），对微生物快速检测方法的需求正在上升，并有望成为卫生检测技术。

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Staining Data

E. coli staining ConditionE. coli culture was stained with 5 mg/ml CTC for 4 hours at 37ºC.A) Phase-contrast microscopeB) fluorescent microscope (485 nm, 510 nm filters)

B. subtiles staining ConditionB. subtilis culture was stained with 5 mg/ml CTC for 4 hours at 37ºC.A) Phase-contrast microscopeB) fluorescent microscope (485 nm, 510 nm filters)