Cell staining mechanism
Staining Procedure1.Prepare 0.5 mg/ml FDA stock solution with DMSO. Dilute 10 ul of the stock solution with 5 ml PBS(-).2.Prepare a cell suspension and wash cells with PBS(-). Prepare 1×105-1×106 cells/ml cell suspension3.Add 15 ul FDA solution to 30 ul cell suspension, and incubate at 37ºC for 15-30 min.4.Put 10 ul stained cell suspension on a glass slide and cover with a cover glass.5.Observe the cells under a fluorescence microscope with 488 nm excitation and 530 nm emission filters.
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