标签：cells

Corning® Tumor Necrosis Factor-a (TNF-a), Human Recombinant, 10µg, 1/Pack 限制进口 货号：354066 品牌：Corning BioCoat

产品介绍： Corning® Tumor Necrosis Factor-a (TNF-a), 10µg, has growth-inhibitory effects on many tumor cells and nontumor cells, e.g., certain hematopoietic cells. The product also has differentiation-inducing effects on macrophages. 1/pack

Source
Recombinant, E. coli

Quality
• Purity: =97% by SDS-PAGE
• Tested for ability to cause cytolysis of murine L929 fibroblasts
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma
• Tested for endotoxin (LAL assay)

Preparation and Storage
Stable for at least three months at -20°C (lyophilized or solubilized). Avoid multiple freeze/thaws. Desiccate lyophilized product.

product details at-a-glance.Qty./Pk.: 1 .Qty./CS.: 1 .Size: 10 µg .Packaging Format: 10µg .Shipping: Refrigerated Shipping

 

 

货号：354066

品牌：Corning BioCoat

规格：10 µg

Corning® Tumor Necrosis Factor-a (TNF-a), Human Recombinant, 10µg, 1/Pack 限制进口 货号：354066      品牌：Corning BioCoat

Corning® Interleukin-4 (IL-4), Human Recombinant354068

产品介绍： Interleukin-4 (IL-4), 5µg, human recombinant, modulates growth and differentiation of a variety of cell types, including B- and T- Lymphocytes, NK cells, mast cells, and bone marrow mast cells. 1/pack

Tip
Recommended concentration: – 0.4 to 2 ng/mL of medium depending on cell type

Source
Recombinant, E. coli

Quality
• Purity: >98% by SDS-PAGE• Shown to stimulate DNA synthesis in human TF-1 cell line (dose-dependent stimulation)• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma

Preparation and Storage
Stable for at least three months at -20°C or = one month at 2° to 8°C (lyophilized or solubilized). Avoid multiple freeze/thaws.

Formulation
Lyophilized from dH2O, carrier-free; reconstitute in dH2O or aqueous buffer

 

Storage
Shelf life: >= 3 months from date of shipment at -20°C or >= one month at 2-8°C (lyophilized or solubilized)

product details at-a-glance.Qty./Pk.: 1 .Qty./CS.: 1 .Size: 5 µg .Packaging Format: 5µg .Shipping: Refrigerated Shipping

 

Corning® Interleukin-4 (IL-4), Human Recombinant, 5µg, 1/Pack 限制进口 货号：354068      品牌：Corning BioCoat

货号：354068

品牌：Corning BioCoat

Corning® Interleukin-3 (IL-3), Mouse Recombinant354058

产品介绍： Corning® Interleukin-3 (IL-3), 10µg, is a glycoprotein that regulates differentiation and enhances the proliferation of a variety of hematopoietic precursor cells, mast cells, basophils, and NK cells. 1/pack

Tip
Recommended dose – 0.1-10 ng/mL of medium depending on cell type.

Source
Recombinant, E. coli

Quality
• Purity: > 95% by SDS-PAGE• Shown to stimulate proliferation of IL-3 dependent murine cells (FDC-P1)• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma

Preparation and Storage
Stable for at least three months at -20°C (lyophilized or solubilized). Avoid multiple freeze/thaws.

Formulation
Lyophilized from dH2O, carrier-free; reconstitute in dH2O or aqueous buffer

Storage
Shelf life: >= 3 months from date of shipment at -20°C (lyophilized or solubilized)

product details at-a-glance.Qty./Pk.: 1 .Qty./CS.: 1 .Size: 10 µg .Packaging Format: 10µg .Shipping: Refrigerated Shipping

 

 

货号：354058

品牌：Corning BioCoat

Corning® Interleukin-3 (IL-3), Mouse Recombinant, 10µg, 1/Pack 限制进口 货号：354058      品牌：Corning BioCoat

Dojindo/CFSE/10/C309,CFSE 标记细胞

DescriptionApplicationReferencesDataS.D.S

CFSE 是细胞膜可渗透的，并且很容易在活细胞内积聚，在那里它与细胞内蛋白质共价结合（图 1）。 水解的 CFSE 发出荧光，共价连接的荧光素分子不会从细胞中泄漏。 CFSE 标记的细胞可以在体内监测数周。 因此，CFSE 可用于检测活细胞以及通过荧光显微镜对细胞活动进行长期观察。 CFSE 标记细胞的激发和发射波长分别为 500 nm 和 520 nm。

Staining Procedure1. Prepare 1 mM CFSE solution with DMSO. Dilute it to prepare 10-50 μM CFSE solution with PBS or an appropriate buffer.2. Add CFSE solution with 1/10 of the volume of cell culture medium to the cell culture.3. Incubate the cell at 37ºC for 15 to 30 min.4. Wash cells twice with PBS or an appropriate buffer.5. Observe the cells under a fluorescence microscope with 490 nm excitation and 530 nm emission filters.

1. M. Bronner-Fraser, Alterations in Neural Crest Migration by a Monoclonal Antibody That Affects Cell Adhesion. J Cell Biol. 1985;101:610-617.2. A. Nose, et al., A Novel Cadherin Cell Adhesion Molecule:Its Expression Patterns Associated WithImplantation and Organogenesis of Mouse Embryos. J Cell Biol. 1986;103:2649-2658.3. S. A. Weston, et al., New Fluorescent Dyes for Lymphocyte Migration Studies Analysis by Flow Cytometry and Fluorescent Microscopy. J Immunol Methods. 1990;133:87-97.4. C. K. Raymond, et al., Molecular Analysis of the Yeast VPS3 Gene and the Role of Its Product in Vacuolar Protein Sorting and Vacuolar Segregation during the Cell Cycle. J Cell Biol. 1990;111:877-892.5. G. Radcliff, et al., Quantification of Effector/Target Conjugation Involving Natural Killer(NK) or Lymphokine Activated Killer(LAK) Cells by Two-color Flow Cytometry. J Immunol Methods. 1991;139:281-292.6. S. A. Weston, et al., Calcein: a Novel Marker for Lymphocytes Which Enter Lymph Nodes. Cytometry. 1992;13:739-749.7. L. S. D. Clerck, et al., Use of Fluorescent Dyes in the Determination of Adherence of Human Leucocytes to Endothelial Cells and the Effects of Fluorochromes on Cellular Function. J Immunol Methods. 1994;172:115-124.

Dojindo细胞分析

细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐，称为WST。WST-8是高度稳定的WST，用于Cell Counting Kit-8（CCK-8）。由于WST-8甲maz是水溶性的，因此不会形成晶体。因此，不需要诸如MTT测定的增溶过程。此外，CCK-8的检测灵敏度高于其他四唑盐，例如MTT，XTT，MTS或WST-1。

WST检测机制

 

ß-半乳糖苷酶检测试剂

细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢

应用 产品展示
细胞生长检测，药物筛选，比色/荧光检测 细胞计数试剂盒-8
细胞计数试剂盒8 + 96孔有机硅定向剂
细胞计数试剂盒-F
细胞毒性LDH检测试剂盒
-WST 96孔有机硅定向剂
MTT
了解检测机制的差异： 点击这里
细胞周期分析 细胞周期测定溶液深红色
细胞周期测定溶液蓝色

 

lonza,2C-101,人脐带血 CD34+ 干/祖细胞

Catalog #: 2C-101

Cryopreserved ampule of Human Cord Blood CD34+ stem/progenitors containing ≥1 million cells

This product is also available with HLA information if your research requires it:
≥1 million cells
≥ 2 million cells

Product Overview

Cord blood CD34+ cells are known to contain hematopoietic stem and progenitor cells, and are ideal for studies relating to transplantation and stem cells as cord blood derived cells tend to be more naïve than similar cells derived from other tissue sources.

众所周知，脐带血 CD34+ 细胞含有造血干细胞和祖细胞，是与移植和干细胞相关的研究的理想选择，因为脐带血来源的细胞往往比其他组织来源的类似细胞更幼稚。

High Purity and Viability

Lonza cord blood CD34+ cells are isolated from cord blood mononuclear cells (MNCs) via positive immunomagnetic separation and are guaranteed to have a purity of ≥90% as indicated by CD34+ expressing cells. They are guaranteed to contain  ≥1 million viable cells per ampoule. HIV-1 and hepatitis B are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Choices in Tissue Source

Cord blood CD34+ cells tend to be more naive than hematopoietic stem cells from other tissue sources. Thus, they can be a great tool when trying to bypass HLA mismatch issues. However, the number of cells available per donor is limited and can pose a challenge in your research. If you are looking for large numbers of hematopoietic stem cells from the same donor, CD34+ cells derived from bone marrow might be a good option.

Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.

Lonza 脐带血 CD34+ 细胞通过阳性免疫磁性分离从脐带血单核细胞 (MNC) 中分离出来，并保证其纯度≥90%，如 CD34+ 表达细胞所示。它们保证每个安瓿含有≥100万个活细胞。并非所有供体和/或细胞批次都检测到 HIV-1 和乙型肝炎。为购买的每个细胞批次提供分析证书。

组织来源的选择

与其他组织来源的造血干细胞相比，脐带血 CD34+ 细胞往往更幼稚。因此，当试图绕过 HLA 不匹配问题时，它们可以成为一个很好的工具。但是，每个供体可用的细胞数量有限，可能会给您的研究带来挑战。如果您正在寻找来自同一供体的大量造血干细胞，来自骨髓的 CD34+ 细胞可能是一个不错的选择。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

Benefits

  • Ready to use
  • Large donor pool
  • High purity with > 90% CD34+ cells

Applications

  • Humanized mouse generation
  • Autoimmune disease
  • Applications requiring more naïve HSCs than those from bone marrow

Content & Storage

Content

1 x Cryopreserved ampule containing ≥1 million cells

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lonza,原代细胞和干细胞,Human Kupffer Cells, 0.5 Million

Catalog #: HLKC
Human Cryopreserved Kupffer Cells
Overview

Product Overview

Kupffer cells are the resident macrophages in the liver and are part of the first line of defense against bacteria and toxins in the gut. Kupffer cells exhibit typical macrophage morphology and can be activated to produce inflammatory cytokines, growth factors and reactive oxygen species.

In vivo, prolonged activation of Kupffers are associated with many diseases of the liver including Non-alcoholic Fatty Liver Disease (NAFLD), Non Alcoholic Steato Hepatitis (NASH), and liver damage associated with certain bioactivated chemicals.

Human Cryopreserved Kupffer cells are isolated and enriched from dissociated human liver tissue and then cryopreserved as passage 0. Characterization of isolated Kupffer cells includes assessing morphology in plated format and evaluating functional responses to LPS stimulation.

Benefits

  • LPS Inducible
  • High activation potential
  • Naive primary cells – never has contacted plastic dish

Applications

  • ADMETox
  • Disease Research

Content & Storage

Content

  • Cryo Vial
  • >0.5 million cells/vial
Instructions, SDS, CoA

Human Kupffer Cells, 0.5 Million

Safety Data Sheets (SDS)

View SDS

SDS – German
SDS – English
SDS – French
SDS – Italian
SDS – Spanish
SDS – Dutch

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Human Kupffer Cells, 0.5 Million

The importance of cell-subtype selection in creation of a 3D liver fibrosis model

Data sheet detailing 3D liver fibrosis model in collaboration with Viskol