标签：CC-5034

lonza,lonza CC-5012,BE02-034E,CC-5034,胰蛋白酶/EDTA 溶液

Catalog #: CC-5012
Trypsin/EDTA 0.025% solution for use in cell culture

Product Overview

Trypsin is a clear, colorless-yellow, highly active bovine enzyme preparation, used to remove cells from the culture vessel surface into suspension.  It is generally used at a working concentration of 0.025%, prepared in a balanced salt solution, without calcium or magnesium at a pH of 7.6-7.8.  After treatment, trypsin activity can be halted by adding a serum media mixture or a trypsin inhibitor (from soybean, for example) in serum-free systems.

Trypsin/EDTA is also a component of Lonza’s ReagentPackTM Subculture Reagents kit (CC-5034) which includes Trypsin Neutralizing reagent and HEPES buffer.

胰蛋白酶是一种透明、无色黄色、高活性的牛酶制剂，用于将细胞从培养容器表面去除成悬浮液。 它通常在 0.025% 的工作浓度下使用，在平衡盐溶液中制备，不含钙或镁，pH 为 7.6-7.8。 处理后，可以通过在无血清系统中添加血清培养基混合物或胰蛋白酶抑制剂（例如来自大豆）来停止胰蛋白酶活性。

胰蛋白酶/EDTA 也是 Lonza 的 ReagentPackTM Subculture Reagents 试剂盒 (CC-5034) 的一个组成部分，其中包括胰蛋白酶中和试剂和 HEPES 缓冲液。

Content & Storage

Content

1 x Bottle Trypsin/EDTA 100 mL

Related Products

Trypsin/EDTA Solution, 100 mL
TrypZeanTM  recombinant Trypsin EDTA (1x) BE02-034E

TrypZeanTM 重组胰蛋白酶 EDTA (1x) BE02-034E

Trypsin/EDTA Solution, 100 mL
ReagentPack™ Subculture Reagents, 100 mL CC-5034
ReagentPack™ 继代培养试剂，100 mL CC-5034

lonza,CC-5002,CC-5034,胰蛋白酶中和溶液100 mL

Catalog #: CC-5002

Trypsin Neutralizing Solution for use in cell culture, 100 mL

Product Overview

Trypsin Neutralizing Solution (TNS) is used to effectively neutralize Trypsin/EDTA. Lonza’s TNS is available  individually or as a component in our ReagentPackTM Subculture Reagents (CC-5034) which includes Trypsin and HEPES buffer.

胰蛋白酶中和溶液 (TNS) 用于有效中和胰蛋白酶/EDTA。 Lonza 的 TNS 可以单独使用，也可以作为我们的 ReagentPackTM Subculture Reagents (CC-5034) 的一个组件使用，其中包括胰蛋白酶和 HEPES 缓冲液。

Content & Storage

Content

1 x Bottle Trypsin Neutralizing Solution, 100 mL

Instructions, SDS, CoA
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Trypsin Neutralizing Solution, 100 mL
ReagentPack™ Subculture Reagents, 100 mL CC-5034
ReagentPack™ 继代培养试剂，100 mL CC-5034

lonza CC-5024,CC-5034,原代细胞和干细胞,HEPES 缓冲盐溶液500 mL

Catalog #: CC-5024
HEPES Buffered Saline Solution for use in cell culture, 500 mL

Product Overview

HEPES 缓冲盐水溶液 (BSS) 用于中和生长培养基中的复杂蛋白质，这些蛋白质可能在胰蛋白酶消化过程中使胰蛋白酶失活。

填充体积为 100 mL 的 HEPES BSS 是 ReagentPackTM Subculture 试剂盒（目录号 CC-5024）的一个组件。 100 mL 容量也单独出售（目录号 CC-5022）。

Content & Storage

Content

1 x Bottle HEPES Buffered Solution, 500 mL

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HEPES Buffered Saline Solution, 500 mL
ReagentPack™ Subculture Reagents, 100 mL CC-5034

ReagentPack™ 继代培养试剂，100 mL CC-5034

HEPES Buffered Saline Solution, 500 mL
HEPES Buffered Saline Solution – 100 ml CC-5022
 HEPES 缓冲盐溶液 – 100 ml CC-5022

lonza CC-5036,CC-5034,原代细胞和干细胞,EBM™- Plus 内皮细胞生长基础培养基-Plus

Catalog #: CC-5036
EBMTM Plus Endothelial Basal Medium

Product Overview

EBMTM Plus is an endothelial basal medium sold separately. This can be used with 1 kit of CC-4542 (endothelial cell growth medium SingleQuotsTM Supplements and Growth Factors for use with HUVEC cells). The full BulletKitTM consisting of basal medium and supplements can be purchased together using the catalog number CC-5035. Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used to grow CloneticsTM Primary Cells.

EBMTM Plus 是一种内皮基础培养基，单独出售。 这可以与 1 套 CC-4542（内皮细胞生长培养基 SingleQuotsTM 补充剂和生长因子用于 HUVEC 细胞）一起使用。 由基础培养基和补充剂组成的完整 BulletKitTM 可以使用目录号 CC-5035 一起购买。 从多种内皮细胞生长培养基中进行选择，以轻松培养原代内皮细胞。 每种内皮细胞生长培养基都有其特殊的配方，并针对某些细胞类型进行了优化。 每种类型的内皮细胞生长培养基在用于培养 CloneticsTM 原代细胞时都能保证发挥作用。


The following Endothelial Cell Growth Medium BulletKitsTM are available:

  • EGMTM-2 BulletKitTM – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Contains 2% FBS and VEGF for rapid proliferation. Does not contain BBE.
  • EGMTMTM BulletKitTM – Optimized for HUVECs cat. no. CC-2517 and CC-2519, contains 2% FBS and BBE. Does not contain exogenous VEGF.
  • EGMTM-Plus BulletKitTM – Enhanced version of EGMTM Medium. Optimized for HUVECs cat. no. CC-2935. Contains 2% FBS and BBE. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGMTM Medium.
  • EGMTM-2 MV BulletKitTM – Coronary and Iliac artery, human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. Does not contain BBE.
  • EGMTM-MV BulletKitTM – Bovine Microvascular Cells, contains 5% FBS and BBE. Does not contain exogenous VEGF.

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

Content & Storage

Content

1 x Bottle Basal Medium 475 mL

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EBM™- Plus Endothelial Cell Growth Basal Medium-Plus
ReagentPack™ Subculture Reagents, 100 mL CC-5034

ReagentPack™ 继代培养试剂，100 mL CC-5034

EBM™- Plus Endothelial Cell Growth Basal Medium-Plus
HUVEC – Human Umbilical Vein Endothelial Cells without VEGF, Single Donor, in EGM™-Plus CC-2935
 HUVEC – EGM™-Plus CC-2935 中不含 VEGF 的人脐静脉内皮细胞，单一供体